Use of Porcine Meal in Plant‐based Practical Diets for Pacific White Shrimp,Litopenaeus vannamei          下载免费PDF全文

Xuan Qiu  D. Allen Davis 《Journal of the World Aquaculture Society》2018,49(3):582-589

Two growth trials were conducted to evaluate and confirm the efficacy of a porcine meal (PM) with high protein content (>90%) as an alternative feed ingredient in commercial‐type feed formulation for Pacific white shrimp, Litopenaeus vannamei. Six experimental diets were formulated for the two growth trials. The first five diets contained increasing levels (0, 1, 2, 4, and 6%) of PM as a replacement for soybean meal in a plant‐based diet with low inclusion level (6%) of fish meal (FM). The last experimental diet was produced utilizing 4.2% PM to completely replace FM. In Trial 1, shrimp (1.5 g initial mean weight, 20 shrimp/tank, n = 4) were offered test diets for 6 wk in a semirecirculation system. At the end of Trial 1, shrimp fed with the diet containing 6% PM exhibited significantly enhanced weight gain (WG), feed conversion ratio (FCR), and survival compared to those fed with the diet devoid of FM. As survival was poor across all treatments and different densities could mask growth results the trial was repeated. In Trial 2, shrimp (0.85 g, 15 shrimp/tank, n = 4) were offered diets for 6 wk. Dietary supplementation of PM at 6% significantly improved WG, FCR, and apparent net protein retention in contrast with the treatment devoid of FM, confirming the same trends in Trial 1. No significant difference was detected in protein, lipid, moisture, and mineral profiles of whole‐body shrimp as well as survival across all the treatments. Results of this study indicate that PM is a good high protein source in shrimp feeds, which can be included up to 6% in the low FM‐based diet without compromising the growth of shrimp.  相似文献   

PEG模拟干旱胁迫下3种鄂尔多斯常见植物萌发期抗旱性评价     

董旋  王明玖  罗冬 《内蒙古草业》2015,(1):34-39

以聚乙二醇溶液(PEG-6000)为渗透介质模拟干旱条件,在12个水势梯度下,研究了干旱胁迫对木地肤、狭叶锦鸡儿和白沙蒿3种植物种子萌发的影响。结果表明,随着PEG-6000浓度的增大,3种植物的发芽率均呈现出明显的下降趋势;而较低浓度的PEG溶液可以对种子萌发产生促进和"刺激"的效果,加快种子萌发的速度,刺激初生根的生长,从而对发芽指数和萌发抗旱指数产生一定的影响。利用隶属函数综合评价3种植物在种子萌发期间的抗旱性,结果为木地肤>白沙蒿>狭叶锦鸡儿。  相似文献   

Delivery of CatSper2 siRNA into Rat Sperms by Electroporation Repressed Ca2＋ Influx During Sperm Hyperactivation     

ZHANG Zhen  ZHOU Xuan  LI  Hui-xia CUI  Qun-wei YU  Jing WANG  Gen-lin 《中国农业科学(英文版)》2011,(12):1958-1967

  相似文献   

施肥量对泰兴香荷芋生长及产量效益的影响     

刘燕敏  焦庆清  徐波  孙玉  秦晓平  谢吉先 《金陵科技学院学报》2014,(3)

通过对泰兴香荷芋施肥量研究,考查了各处理主要生育期的苗情与长势、成熟期子孙芋产量及商品芋产值效益。结果显示:基施腐熟脱水猪粪15t·hm-2,苗期追施(3.1叶)脱水猪粪15t·hm-2+13-10-18高钾复合肥675kg·hm-2的处理子孙芋产量最高为23 421.90kg·hm-2,商品芋效益为88 506.60元·hm-2;基施有机肥7 500kg·hm-2+苗期追施(3.1叶)有机肥7 500kg·hm-2+13-10-18高钾复合肥450kg·hm-2+膨大肥(8.1叶)13-10-18高钾复合肥900kg·hm-2的处理产量相对较高。  相似文献   

Two Complete Mitochondrial Genomes From Leuctridae (Plecoptera: Nemouroidea): Implications for the Phylogenetic Relationships Among Stoneflies     

Jinjun Cao  Ying Wang  Xuan Guo  Guoquan Wang  Weihai Li  Dvid Murnyi 《Journal of insect science (Online)》2021,21(1)

The family-level relationships within Plecoptera have been a focused area of research for a long time. Its higher classification remains unstable, and the phylogenetic relationships within Plecoptera should be re-examined. Here, we sequenced and analyzed two complete mitochondrial genomes (mitogenomes) of Paraleuctra cercia and Perlomyia isobeae of the family Leuctridae. We reconstructed the phylogeny of Plecoptera based on 13 protein-coding genes (PCGs) from published stoneflies. Our results showed that the Bayesian inference and maximum-likelihood tree had similar topological structures except for the positions of two families, Peltoperlidae and Scopuridae. The Plecoptera is divided into two clades, the suborder Antarctoperlaria and the suborder Arctoperlaria. The two suborders subsequently formed two groups, Eusthenioidea and Gripopterygoidea, and Euholognatha and Systellognatha, which is consistent with the results of morphological studies. In addition, the Leuctridae is the earliest branch within the superfamily Nemouroidea. But the monophyly of Perloidea and Pteronarcyoidea are still not well supported.  相似文献   

黔育1号菊苣与紫花苜蓿、鸭茅间作栽培效果研究     

覃涛英  付薇  苏生  陈伟  韩永芬  李德芳  马宁  马培杰 《畜牧与饲料科学》2019,40(7):55-59

依据贵州省松桃县的气候特点和牧草种植需要,用黔育1号菊苣与北林202紫花苜蓿、黔草4号鸭茅分别进行间作试验,从产量、营养品质、生产效益等方面对间作复合群体与3种牧草单播进行比较分析,探讨黔育1号菊苣与不同牧草间作种植模式的增产效益。结果表明,在贵州松桃地区,黔育1号菊苣+北林202紫花苜蓿间作,年均鲜草产量可达87.27 t/hm²,比紫花苜蓿单播产量增加36.17%,生产效益提高20.77%,粗蛋白含量达26.83%。该研究结果可为松桃县种草养畜产业的发展提供技术支撑。  相似文献   

不同厂家阿胶及动物皮胶中四种阴离子含量的测定     

刘雯  李峰  覃洁萍  冯旭 《湖北农业科学》2020,59(8):134-137

通过离子交换色谱法,建立不同厂家阿胶及动物皮胶中Cl~-、SO_4~(2-)、NO_3~-、PO_4~(3-)4种阴离子的测定方法。结果表明,4种阴离子分离效果良好,Cl~-、SO_4~(2-)、NO_3~-、PO_4~(3-)分别在0.000 922～0.023 1、0.001 09～0.027 3、0.000 167～0.004 18、0.000 314～0.007 86 mg/mL浓度范围内线性关系良好,平均加样回收率在98.7%～101.0%。该测定方法结果准确、灵敏、可靠,重现性较好,可用于阿胶及动物皮胶中4种阴离子含量的测定。  相似文献   

Infections Investigation of ALV and REV in Guangxi Local Poultry Breeds and Sequence Analysis of gp85 Gene of ALV Isolates     

ZHONG Xing-fu  WANG Pei-kun  YANG Yong-li  QIN Li-li  BI Yu-yu  ZOU Guang-zhen  PENG Hao  WEI Ping 《中国畜牧兽医》2015,42(7):1647-1653

In order to investigate the infection status of avian leukosis virus (ALV) and avian reticuloendotheliosis virus (REV) in the major local breeds of Qinzhou,Guangxi,totally 953 samples of egg white,cloaca swab and serum of Ma duck,Shitou goose,Tiejiao-Ma chicken,turkey and pigeon were collected from the representing flocks and detected by the commercial ELISA kits.ALV was isolated for the ALV p27 positive samples by culturing on DF-1 cells,and gp85 gene was sequenced.The results showed that the detections of ALV were negative in the samples except those of Tiejiao-Ma chicken,while REV antibody was found positive in Ma duck,Tiejiao-Ma chicken and turkey.The nucleotide sequences of gp85 gene of two isolates shared 94.5% identity with each other,and shared 86.9% to 94.9% with reference strains.The amino acid sequences of gp85 gene of two isolates shared 91.5% identity with each other,and shared 84.0% to 91.6% with reference strains.There were many variable sites in the hyper variable region hr1 and hr2,and the vr2 and vr3 variable regions were relatively conservative.Phylogenetic tree analysis showed that the two isolates shared the highest homology with SCAU11-XG strain.  相似文献   

Cloning and Sequence Analysis of Buffalo Keap1 Gene and Investigation of Its Expression Pattern in Different Tissues     

ZHAO Xin  RUAN Zi-yun  GUO Zhen-wei  ZHOU Wen-ting  QIN Xi-ling  NIU Xiang-li  LU Feng-hua  SHI De-shun 《中国畜牧兽医》2016,43(8):1975-1982

In this study,the CDS sequence of buffalo Keap1 gene was cloned and analyzed,then its expression pattern in different tissues was also investigated.A pair of primers of buffalo Keap1 gene was designed based on the nucleotide sequence of Bos taurus Keap1 gene from GenBank,and then the buffalo Keap1 gene was amplified.Using the bioinformation techniques,the gene sequence and the protein structure were analyzed.The expression level of Keap1 gene in different tissues were detected with Real-time quantitative PCR.The results showed that the length of buffalo Keap1 gene coding sequence was 1 875 bp and encoded 624 amino acids.The multiple sequence alignment results showed that buffalo Keap1 gene shared 99%,96%,92% and 90% of similar nucleotide sequence with that of Bos taurus,Ovis aries,Sus scrofa and Homo sapiens,respectively.And the phyogenetic tree also showed the conservatism between several different species.The second structure of buffalo Keap1 protein was predicted as 24 alpha regions,40 beta regions,38 turn regions and 27 coil regions.In addition,the results of Real-time quantitative PCR showed that Keap1 mRNA exists in all seven tissues,but the most abundant expression was in heart and the minimal expression was in liver and spleen.The results provided an foundation for further study of Keap1-Nrf2-ARE signal pathway,for enhancing the ability of antioxidant of buffalo embryo in vitro culture.  相似文献   

汉中市耐寒桉树引种繁殖试验   总被引：2，自引：0，他引：2  

刘彦伟  马玲  衡保清  秦珊 《陕西林业科技》2014,(4)

桉树是目前世界上发展最快的短轮伐期工业原料林树种,但多数不耐寒,适宜在热带和亚热带地区发展。为了扩大其北移引种,从四川省林科院引种耐寒品种开展抗寒、抗逆性研究,初步能在汉中安全越冬。本文以邓恩桉全光喷雾嫩枝扦插试验为例,介绍了耐寒桉树繁殖技术。试验采用4种基质、吲哚丁酸3个处理、1个对照、3个重复,结果表明:扦插不同基质处理以珍珠岩∶腐质土(4∶6)最好,吲哚丁酸不同浓度处理间差异不显著,以浓度1 000mg·L-1最好,平均生根率最高达70.83%。  相似文献