Quantitation of adenine nucleotides in equine colonic mucosal tissue using high performance liquid chromatography.     

J Tetens  S A Barker  M Waguespack    G Hosgood 《Canadian journal of veterinary research》2001,65(2):133-135

The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period.  相似文献   

Transmission of porcine reproductive and respiratory syndrome virus from persistently infected sows to contact controls.   总被引：3，自引：0，他引：3       下载免费PDF全文

M D Bierk  S A Dee  K D Rossow  S Otake  J E Collins    T W Molitor 《Canadian journal of veterinary research》2001,65(4):261-266

The objective of this study was to determine if porcine reproductive and respiratory syndrome virus (PRRSV) could persist in non-pregnant sows and if persistently infected sows could transmit virus to naive contact controls. Twelve PRRSV-naive, non-pregnant sows (index sows) were infected with a field isolate of PRRSV and housed in individual isolation rooms for 42 to 56 days postinfection. Following this period, 1 naive contact sow was placed in each room divided by a gate allowing nose-to-nose contact with a single index sow. Index sows were not viremic at the time of contact sow entry. Virus nucleic acid was detected by polymerase chain reaction, and infectious virus was detected by virus isolation in sera from 3 of the 12 contact sows at 49, 56, and 86 days postinfection. All 3 infected contacts developed PRRSV antibodies. Virus nucleic acid was detected in tissues of all of the 12 index sows at 72 or 86 days postinfection. Nucleic acid sequencing indicated that representative samples from index and infected contacts were homologous (> 99%) to the PRRSV used to infect index sows at the onset of the study. This study demonstrates that PRRSV can persist in sows and that persistently infected sows can transmit virus to naive contact animals.  相似文献   

胸神经后皮支及其营养血管的解剖学研究     

梁大宁  罗少军  汤少明  吕端远  张新营 《湛江医学院学报》2002,20(6):415-417

目的：为临床设计背部皮神经营养血管皮瓣提供解剖学基础。方法：在l7例34侧成人躯干标本上，用显微解剖法观测第l—l2胸神经后皮支及其营养血管的来源．走行及分布规律。结果：第l—l2胸神经后皮支的伴行动脉为肋间后动脉的背侧皮支。皮神经及其伴行动脉于脊柱两旁相应肋间穿出深筋膜，呈节段性、重叠性分布于背部皮肤，皮神经营养血管相互间形成丰富的吻合。结论：可设计以第l—12胸神经后皮支及其营养血管为蒂的皮神经营养血管皮瓣。  相似文献   

轻型木结构建筑覆板的集中静载性能   总被引：2，自引：1，他引：1  

王戈  费本华  林利民  唐伟  徐兰英 《木材工业》2005,19(4):9-11

介绍轻型木结构建筑覆板集中静载结构性能试验的装置、条件和方法,并进行国产人工林落叶松胶合板作轻型木结构覆板的集中静载性能研究.结果表明:与国外胶合板覆板具有相同厚度的国产落叶松屋面板和楼面板,其集中载荷性能均优于相关标准的要求.  相似文献   

红曲霉色素的提取     

张文芳  蒋光正  李薇  刘若男 《东北农业大学学报》1990,(1)

本实验确立了一种新的红曲霉色素提取方法:以红曲米为原料,乙醇为提取剂,得到的提取液再以正己烷提取分离。结果表明,此法所得的红曲霉色素是水溶性的,宜于用作食品添加剂。此外,还对影响提取的因素进行了探讨,并提供了最佳提取方案。  相似文献   

Outcome of limb amputations in wapiti: 13 cases (1995-2001).          下载免费PDF全文

T D Butt  A M Cruz  J V Bailey    W H Crawford 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(12):936-939

The object of this investigation was to determine the outcome of limb amputation in wapiti. Medical records of 13 wapiti that underwent limb amputation were reviewed to determine age, weight, sex, injury preceding amputation, limb amputated, amputation location, length of hospital stay, complications, and outcome. Ages ranged from 3 months to 8 years and weights from 70 kg to 280 kg. All animals were female. Eleven animals sustained catastrophic long bone fractures prior to amputation. Five animals had front limb amputations and 8 had hind limb amputations. Of these animals, 1 with a front limb amputaiton and 5 with hind limb amputations survived. Postsurgical inability to stand is associated with hospitalization of > 1 day. Of the 13 wapiti reviewed, 5 with hind limb amputations have produced and reared offspring. Hind limb amputation can be considered a viable alternative to euthanasia when catastrophic orthopedic injuries occur in female wapiti.  相似文献   

离心式压缩机喘振问题分析     

唐善华  薛献忠 《油气储运》2004,23(1):36-38

当压缩机内气体的流量较低时,气体容易出现旋转脱离现象,导致压缩机不能正常工作,采取增加压缩机内部液体的流量,在出口处设置单向阀防止气体倒流等措施,可有效地防止喘振的发生,保证输气管道的正常运行.  相似文献   

Online First publication     

Springer-Verlag  The Editor-in-Chief 《Biology and Fertility of Soils》2001,33(1):1-1

  相似文献   

压力实时监测法在管道泄漏检测中的应用   总被引：2，自引：0，他引：2  

靳春义  宋春花 《油气储运》2001,20(8):48-49

检测输油管道漏油的方法很多，介绍了通过压力实时监测确定输油管道漏油点方法，以及实际应用情况。实践证明，通过压力实时监视确定输油管道漏油点的方法在实际输油运行中是切实可行的。  相似文献   

图书馆业务外包的实施策略   总被引：12，自引：0，他引：12  

唐定海 《农业图书情报学刊》2004,16(12):89-91

业务外包是一种新型的图书馆管理方法。本文介绍了图书馆业务外包的概念、内容、作用，探讨了实施图书馆业务外包的相应策略。  相似文献