基于小波分析的玉米籽粒图像正形研究   总被引：9，自引：3，他引：9  

权龙哲  马小愚 《农机化研究》2006,(2):154-156

近年来,通过对玉米籽粒的尺寸、外形、色泽等方面的研究,已获得了一些必要的单籽粒的视觉信息。但是,对于粮食作物而言,只有实现了作物籽粒的大规模、快速识别,才有可能在实际生产中得到广泛应用。为此,采用图像标记法,对含有众多的、散放的玉米籽粒图像进行处理,对标记图像中的像素进行逻辑判断,采用多尺度小波分析算法,实现籽粒分割、定位及正形。  相似文献   

客车车身结构强度及刚度分析     

蔺瑞兰  陈玉杰 《农业装备与车辆工程》2007,5(1):27-29

用有限元法对半承载式客车车身强度、刚度进行了分析，用电测量技术对有限元模型进行了验证。为改进设计提供了理论依据，分析结果可作为车身骨架结构优化的参考。  相似文献   

干旱地区家庭雨水集储装置的设计     

刘兰桂  全腊珍  汤兴初 《农机化研究》2004,(3):115-117

宁夏甘肃两省是我国严重缺水地区。缺水严重影响了该地区人民正常生活和工农业的发展。为此，针对家庭用水设计了屋顶雨水集储装置；在尽量减少建筑费用的情况下，选用了地下建池、拦污栅等污水处理装置，可为干旱地区建造家庭雨水集储装置提供一些参考依据。  相似文献   

地被菊'映洁红菲'再生及遗传转化体系的建立     

全英杰  任镘蓉  杨雯婷  张佳凝  何子涵  高日 《北方园艺》2021,(21):72-77

以地被菊'映洁红菲'试管苗叶片为试材,采用组织培养法,研究了6-苄基腺嘌呤、萘乙酸激素浓度、蔗糖浓度、MS浓度、NO3-和NH+的浓度比、羧苄青霉素浓度及潮霉素浓度对不定芽分化的影响,以期建立'映洁红菲'高效的再生体系,为地被菊转基因体系奠定基础.结果 表明:在6-BA和NAA浓度分别为2.0、0.6 mg·L-1,蔗糖浓度为30 g·L-1,NO3-:NH4+为30∶30,基本培养基3/4MS中,'映洁红菲'叶片中愈伤组织诱导和不定芽分化率达到最高,平均每个叶盘分化3.22个不定芽,诱导率75.67％.在生根培养基中加入浓度为0.75 mg·L-1 IBA时,根系生长最好.'映洁红菲'遗传转化时,羧苄青霉素浓度为250mg· L-1,潮霉素浓度为2.0 mg·L-1,适合作为选择压.  相似文献   

Single nucleotide polymorphisms of TLR4 locus in Chinese Cantonese population     

XIAO Wen-juan  LI Nan  GONG Ying-xue  WANG Jun-mei  QUAN Yan-cai  LIU Ze-huan△ 《园艺学报》2010,26(6):1193-1197

AIM: Toll-like receptor 4 (TLR4) was an important pathogen recognition receptor in the innate immune system. The aim of this study was to investigate the distribution of TLR4 polymorphisms in the general population of China. METHODS: Peripheral blood samples were collected from 191 unrelated healthy Chinese Cantonese individuals. The functional regions of TLR4 locus, including promoter region and all three exons with their surrounding intronic regions were amplified using polymerase chain reaction. After purified, the amplified products were directly sequenced on both strands. RESULTS: A total of eight single nucleotides polymorphisms (SNPs) were detected, five of which were novel. The most common SNP were -1607 C/T with the minor allele frequency of 0.283. Two nonsynonymous substitutions Asp299Gly and Thr399Ile, which were common in Caucasus, were not detected in Cantonese. Neutrality test revealed that TLR4 in Chinese Cantonese was not significantly deviated from the neutral model. CONCLUSION: This is the new finding on the distribution of TLR4 SNPs in the general population of China. It provides several ethnic specific SNPs for further disease association studies of TLR4 polymorphisms in Chinese populations.  相似文献   

牛乳头状瘤的病理学观察及其病毒基因型分析   总被引：1，自引：0，他引：1  

梁辰  姜晓文  刘旭  权鑫  于文会 《中国预防兽医学报》2013,35(5)

为研究牛乳头状瘤病毒(BPV)在我国对牛的致病性及基因型,本研究选取某奶牛场发病的犊牛,通过对皮肤肿瘤样物进行临床观察、组织病理学及免疫组织化学检查,并应用DNA提取及PCR等技术对其进行鉴定.组织病理学结果显示牛乳头状瘤呈现角化过度和细胞空泡化现象；免疫组织化学结果显示胞浆棕染呈BPV阳性；应用PCR对其DNA进行扩增,检测出阳性样品,测序结果表明与BPV-1和BPV-2同源性达98％～99％.表明该奶牛场犊牛发病由BPV-1和BPV-2引起的.  相似文献   

植物病害生物防治与微生态学   总被引：11，自引：0，他引：11  

王万能  全学军  谢应宇 《中国植保导刊》2005,25(3):7-9

在概述植物病害生物防治生态学意义的基础上,从植物微生态学的角度阐述了植物根围菌及内生菌作为生防菌的依据和意义。特别是内生菌是植物体内的正常菌群,对植物病害生物防治具有重要的生态学实践意义。  相似文献   

Peripheral blood mononuclear cells activated by graded zirconia-hydroxyapatite composite in vitro     

QUAN Ren-fu  YANG Di-sheng  MIAO Xu-dong  LI Wei  WU Xiao-chun  WANG Hong-bin 《园艺学报》2006,22(1):160-163

AIM: To evaluate the immunogenicity of a novel orthopedics materials (graded zirconia-hydroxyapatite composite) in vitro by using peripheral blood mononuclear cells (PBMCs) from healthy young people, and simple zirconia-hydroxyapatite composited material was used as control materials. METHODS: Proliferation of PBMCs cultured in different liquid after 5 days was measured by MTT methods. ELISA was used to detect TNF-α and IL-6 concentration in the supernatant of PBMCs cultured in the extracts after 24 hours. Flow cytometery was used to measure CD69 and CD25 in activated PBMCs cultured in the extracts of the two kinds of materials after 24 hours. RESULTS: The proliferation rate of the simple composite group was significantly lower than that in negative group (P<0.05), but there was no difference between the graded composited group and negative group. After 24 hours culture with LPS, the concentrations of TNF-α and IL-6 in the simple composited group were significantly higher than that in graded composited group, respectively (P<0.05). Cultured with PHA for 24 hours, the ratio of CD69 and CD25 positive PBMCs in the simple composited group was all significantly higher than that in graded composited material group (P<0.01). CONCLUSION: The number of PBMCs activated by the graded composited material is less than the simple composited material and the immunogenicity of the graded composited material is lower than the simple composited material.  相似文献   

转EGFP基因山羊克隆胚的发育     

郑月茂  刘凤军  安志兴  李向臣  赵晓娥  权富生  张涌 《畜牧兽医学报》2007,38(5):458-463

利用脂质体包裹含EGFP基因的质粒,并将之导入山羊乳腺上皮细胞,经G418筛选获得阳性细胞,以阳性细胞作为核供体,利用核移植技术构建转基因克隆胚。结果表明：用转基因山羊乳腺上皮细胞作为核供体,电融合法更适合构建转基因克隆胚。转基因山羊克隆胚体外培养最佳方案是用SOFaa培养液,在培养72 h后加入10%的正常山羊血清（Normal goat serum,NGS）。荧光显微镜下观察到转基因克隆胚中EGFP的表达,大部分克隆胚发育到8-16细胞以后的时期,绿色荧光蛋白才开始逐渐表达,随着发育时间的延长,绿色荧光蛋白的表达也逐渐增强。说明外源基因在胚胎早期发育阶段可以表达,EGFP可以作为报告基因来实现对外源基因整合及表达的监测。  相似文献   

广西柳江县糖料蔗农业生产情况调查     

王宏卫  张仲宪  贤振华  杨幼  全志强 《中国糖料》2009,(3):44-47

主要通过调查问卷和实地考察的形式对广西柳江县7个乡镇65个村的糖料蔗农业生产多方面情况进行了调查,分析了该县糖料蔗农业生产中存在的问题和限制因子,为该县糖料蔗健康稳定的发展提出合理的建议和对策.  相似文献