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91.
1. To detect polymorphism in various quail lines, Randomly Amplified Polymorphic DNA (RAPD) markers were tested and were found to be effective. 2. Twenty decamer primers were selected at random and tested, and 6 of these generated distinct polymorphic patterns between the quail lines. 3. Out of a total of 60 bands amplified using 6 selected primers, 19 (31.7%) were found to be polymorphic. 4. Genetic similarity within the lines ranged from 0.726 in HBW to 0.836 in KLQ. 5. Genetic similarity estimates between the populations ranged from 0.709 to 0.808. 相似文献
92.
Bridges PJ Wright DJ Buford WI Ahmad N Hernandez-Fonseca H McCormick ML Schrick FN Dailey RA Lewis PE Inskeep EK 《Journal of animal science》2000,78(11):2942-2949
Experiments were conducted in beef cows without a primary CL, in which pregnancy had been maintained with exogenous progestogen. In preliminary trials, replacement CL induced ipsilateral to the embryo and after, rather than before, d 36 of pregnancy, maintained more pregnancies after withdrawal of exogenous progestogen (13/13 vs 2/6; P < 0.05). In Exp. 1, in cows with replacement CL induced by treatment with hCG on d 28 of pregnancy, treatment with flunixin meglumine on d 31 through 37 did not increase maintenance of pregnancy. Experiment 2 was conducted to evaluate directly the effects of concentrations of PGF2alpha and estradiol-17beta during d 31 through 35 of pregnancy on maintenance of pregnancy by replacement CL induced between d 28 and 31. In cows that maintained pregnancy while progestogen was provided, maintenance of pregnancy after withdrawal of exogenous progestogen tended to be greater with high (5/5) than with low (2/6; P < 0.10) concentrations of PGF2alpha and greater with low (6/7) than with high (2/6; P = 0.10) concentrations of estradiol-17beta. Secretion of progesterone by replacement CL was greater (P < 0.05) in cows with high than in those with low concentrations of PGF2, during d 31 through 35. Prostaglandin F2alpha may facilitate attachment of the bovine embryo (d 30 to 40) in a manner similar to that reported for implantation in other species. Cows that did not form CL in response to hCG on d 28 to 31 responded well when retreated after d 36. Again, maintenance of pregnancy was greater when replacement CL were induced after (9/9) rather than before d 36 (8/16; P < 0.05). 相似文献
93.
发酵血粉代替豆粕对育肥猪的影响 总被引:1,自引:0,他引:1
试验采用单因子试验设计,选用40kg左右的肥育猪40头,随机分成4组,每组5个重复,每个重复2头,分别饲喂基础日粮(对照组)和用发酵血粉分别替代基础日粮中的豆粕50%(试验Ⅰ组)、75%(试验Ⅱ组)和100%(试验Ⅲ组)等4种试粮,进行60d试验。结果表明:与对照组相比,试验Ⅱ组和试验Ⅲ组的末重与日增重均有显著提高(P<0.05),其中日增重分别提高11.33%和14.34%;与对照组相比,试验Ⅱ组料重比显著降低(P<0.05),其它各组差异不显著(P>0.05)。代谢试验表明:与对照组相比,蛋白质的消化率,氮沉积量(g/d)、氮沉积率(g/kgBW0.75)都显著提高(P<0.05)。随着替代的比例增加,经济效益改善呈上升趋势,试验Ⅱ组和试验Ⅲ组分别比对照组多赢利23.11%和28.29%。 相似文献
94.
95.
The roots of 20 days old seedlings of tomato (Lycopersicon esculentum Mill.) at the time of their transplantation, were dipped in 10−8, 10−7, 10−6 or 10−5 M of 28-homobrassinolide (HBR) for 15, 30 or 45 min and were allowed to grow in earthen pots, in a net house. The leaves of the plants, at days 30 and 60, possessed elevated quantities of nitrate reductase (NR), carbonic anhydrase (CA) and the contents of chlorophyll. The values for all the above characteristics were significantly higher than that of the water-fed control. The fruits borned at the treated plants were more in number and possessed a lower quantity of ascorbic acid than the control. Moreover, the fruits at ripening, had higher levels of lycopene and β-carotene. Among the treatments, 15 min feeding of 10−8 M HBR proved best. 相似文献
96.
97.
调查表明,东乡野生稻原产保护地蜘蛛群落优势科为狼蛛科、跳蛛科、管巢蛛科、球蛛科、肖蛸科,相对丰度分别为0.5010、0.1529、0.0785、0.0657、0.0559、0.0399、0.0392,其丰盛度总和达0.8540,构成了蜘蛛群落的主要成分;巨蟹蛛科为稀有科。优势种为拟水狼蛛、沟渠豹蛛、中华狼蛛、横窝希蛛、黄斑纯蛛、白纹舞蛛、微菱头蛛、柔弱锯螯蛛,其相对丰盛度分别为0.1802、0.1209、0.0833、0.0589、0.0484、0.0445、0.0417、0.0413,丰盛度总和达0.6192,埃比熊蛛等为稀有种。调查还表明,不同时期的物种丰富度变化不明显,但在6~7月间,群落中物种丰富度相对较高,各物种种群发生数量大,分布相对均匀,优势种不突出,群落结构较合理,均匀度和多样性指数较高。 相似文献
98.
ABSTRACT Taxonomically distinct tobacco mosaic tobamovirus (TMV), red clover necrotic mosaic dianthovirus (RCNMV), cucumber mosaic cucumovirus (CMV), brome mosaic bromovirus (BMV), and cowpea chlorotic mottle bromovirus (CCMV) exhibit differences in their host range. Each of these viruses encodes a functionally similar nonstructural movement protein (MP) that is essential for cell-to-cell movement of a progeny virus. Despite the lack of significant amino acid identity among the MPs of CMV, TMV, and RCNMV, movement-defective CMV (CMVFnyDeltaMP-DeltaKPN) was able to move locally and systemically in transgenic Nicotiana benthamiana expressing either TMV MP (NB-TMV-MP(+)) or RCNMV MP (NB-RCNMV-MP(+)). These observations contrast with those of previous studies in which transgenic N. tabacum cv. Xanthi plants expressing TMV MP supported only the cell-to-cell movement of CMVFnyDeltaMP-DeltaKPN. To verify whether similar complementation could be observed for movement-defective bromoviruses, NB-TMV-MP(+) and NB-RCNMV-MP(+) plants were inoculated independently with movement-defective variants of BMV (B3DeltaMP) and CCMV (CC3DeltaMP). Neither NB-TMV-MP(+) nor NB-RCNMV-MP(+) was able to rescue the defective cell-to-cell and long-distance movement of B3DeltaMP. In contrast, NB-RCNMV-MP(+) complemented the cell-to-cell, but not the long-distance, movement of CC3DeltaMP. Taken together, these studies suggest that virus movement is a complex process and that, in some cases, the host species plays a major role in determining the long-distance movement function of a virus. 相似文献
99.
The isolation and NMR spectra of otosenine (1) and seneciphylline (2) from Senecio lorenthii are reported. 相似文献
100.
Four male buffaloes of 3 to years were subjected to heavy loads during hot dry conditions for 3 h continuously. Rectal temperature, respiration rate and heart rate increased significantly. The blood pH, PO2, increased significantly and PCO2 declined. During work, buffaloes were not able to eliminate heat produced and exhibited such distress symptoms as profuse salivation, frothing, lack of leg co-ordination, excitement and tongue protrusion. The muzzle secretion of buffaloes increased greatly. Even 3 h after discontinuation of work, the physiological reactions had not fully disappeared. 相似文献