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61.
62.
63.
Long-term persistence of multi-drug-resistant Salmonella enterica serovar Newport in two dairy herds
Cobbold RN Rice DH Davis MA Besser TE Hancock DD 《Journal of the American Veterinary Medical Association》2006,228(4):585-591
OBJECTIVE: To evaluate the association between maintaining joint hospital and maternity pens and persistence of multi-drug-resistant (MDR) Salmonella enterica serovar Newport on 2 dairy farms. DESIGN: Observational study. SAMPLE POPULATION: Feces and environmental samples from 2 dairy herds. PROCEDURE: Herds were monitored for fecal shedding of S enterica Newport after outbreaks of clinical disease. Fecal and environmental samples were collected approximately monthly from pens housing sick cows and calving cows and from pens containing lactating cows. Cattle shedding the organism were tested serially on subsequent visits to determine carrier status. One farm was resampled after initiation of interventional procedures, including separation of hospital and maternity pens. Isolates were characterized via serotyping, determination of antimicrobial resistance phenotype, detection of the CMY-2 gene, and DNA fingerprinting. RESULTS: The prevalence (32.4% and 33.3% on farms A and B, respectively) of isolating Salmonella from samples from joint hospital-maternity pens was significantly higher than the prevalence in samples from pens housing preparturient cows (0.8%, both farms) and postparturient cows on Farm B (8.8%). Multi-drug-resistant Salmonella Newport was isolated in high numbers from bedding material, feed refusals, lagoon slurry, and milk filters. One cow excreted the organism for 190 days. Interventional procedures yielded significant reductions in the prevalences of isolating the organism from fecal and environmental samples. Most isolates were of the C2 serogroup and were resistant to third-generation cephalosporins. CONCLUSIONS AND CLINICAL RELEVANCE: Management practices may be effective at reducing the persistence of MDR Salmonella spp in dairy herds, thus mitigating animal and public health risk. 相似文献
64.
The use of antimicrobial drugs in livestock is suspected to contribute to bacterial antimicrobial resistance (AR) development. Dairy farms experiencing recent outbreaks of salmonellosis involving multi-resistant (MR) Salmonella strains were compared to control farms with respect to AR among bovine commensal E. coli isolates. For most antimicrobials tested, the percentage of AR E. coli isolated from salmonellosis-affected farms was significantly higher than that from control farms. Calf E. coli from both case and control farms had greater levels of AR than cow isolates. Commensal E. coli isolates from case farms and calves tended to more frequently be MR. These data are consistent with the existence of higher antimicrobial selection pressure on farms with recent salmonellosis outbreaks, however, the directionality of the relationship remains to be elucidated. An improved understanding of the epidemiology of AR bacteria in livestock production, both at the herd and molecular level, is essential to mitigate risk to public health and food safety. 相似文献
65.
T.J. Gruffydd-Jones B.R. Jones H. Hodge M. Rice M.A. Gething 《New Zealand veterinary journal》2013,61(5):201-203
Conjunctival swabs collected in 1991-92 from 333 pedigree and non-pedigree cats were tested for the presence of Chlamydia spp. antigen using an ELISA antigen kit. Forty (18.4%) of the 217 samples from cats with conjunctivitis were positive. Seven (6%) of 116 samples from cats which were in contact with cats with conjunctivitis but which showed no clinical signs at the time of sample collection were positive. Positive-testing cats were frequently from multi-cat households. Chlamydia spp. is present and associated with conjunctivitis in cats in New Zealand. Infection may occur concurrently with viral diseases. Feline calicivirus was recovered from 27 (21 with conjunctivitis) of 37 cats tested in five catteries. Four cats (with conjunctivitis) were FIV-positive. 相似文献
66.
Richard W. Todd N. Andy Cole R. Nolan Clark William C. Rice Wen-Xuan Guo 《Biology and Fertility of Soils》2008,44(8):1099-1102
Cattle feedyards can impact local environments through emission of ammonia and dust deposited on nearby land. Impacts range
from beneficial fertilization of cropland to detrimental effects on sensitive ecosystems. Shortgrass prairie downwind from
an adjacent feedyard on the southern High Plains of Texas, USA changed from perennial grasses to annual weeds. It was hypothesized
that N enrichment from the feedyard initiated the cascade of negative ecological change. Objectives were to determine the
distribution of soil nitrogen and estimate N loading to the pasture. Soil samples were collected from 119 locations across
the pasture and soil total N (TN), nitrate-N and ammonium-N (AN) determined in the top 30 cm. Soil TN concentration decreased
with distance downwind from the feedyard from 1.6 ± 0.2 g kg−1 at 75 m to 1.2 ± 0.05 g kg−1 at 582 m. Nitrate-N concentration decreased within 200 m of the feedyard and changed little at greater distances. Ammonium-N
concentration decreased linearly (P < 0.001) with increasing distance from the feedyard from 7.9 ± 1.7 mg kg−1 within 75 m from the feedyard to 5.8 ± 1.5 mg kg−1 at more than 550 m from the feedyard; however, distance only explained 12% of the variability in AN concentration. Maximum
nitrogen loading, from 75 to 106 m from the feedyard, was 49 kg ha−1 year−1 over 34 years and decreased with distance from the feedyard. An estimate of net dry deposition of ammonia indicated that
it contributed negligibly to N loading to the pasture. Nitrogen enrichment that potentially shifted vegetation from perennial
grasses to annual weeds affected soil N up to 500 m from the feedyard; however, measured organic and inorganic N beyond that
returned to typical and expected levels for undisturbed shortgrass prairie.
相似文献
Richard W. ToddEmail: |
67.
Abderrazek Jilal Stefania Grando Robert J. Henry L. Slade Lee Nicole Rice Helen Hill Michael Baum Salvatore Ceccarelli 《Genetic Resources and Crop Evolution》2008,55(8):1221-1230
Twenty genic- and genomic SSR markers were used to study genetic diversity and geographical differentiation of barley from 29 countries through analysis of a worldwide collection of 304 ICARDA’s barley landraces. Of these, 19 loci were highly polymorphic in the material studied. Based on Nei-distance matrix, Principal Component Analysis (PCoA) and cluster analysis using UPGMA associated with AMOVA the data revealed countries’ grouping within regions. Three distinct germplasm pools were identified in the landraces. The first of these was from Eastern Africa (Eritrea and Ethiopia) and South America (Ecuador, Peru and Chile) suggesting that barley introduced to South America might have originated specifically from East Africa or that they share a common genetic basis for adaptation. The second was the Caucasus (Armenia and Georgia) and the third included the remaining regions of Central Asia, Near East, Northern Africa and Eastern Asia. Genetic diversity of barley subspecies (Six-rowed barley, Two-rowed barley, H. spontaneum C. Koch and H. agriocrithon Åberg) also discriminates them into three groups: cultivated barleys (Six-rowed barley and Two-rowed barley), wild barley H. spontaneum and subspecies H. agriocrithon. These results associated with parsimony analysis demonstrate that H. agriocrithon and H. spontaneum might be distinct and do not support a hybrid origin for H. agriocrithon suggesting further investigation of the basis of more intense sampling of the two subspecies H. spontaneum and H. agriocrithon. 相似文献
68.
Faecal samples from 51 cats were examined by negative stain electron microscopy to investigate a possible association between the syndrome of bilateral protrusion of the nictitating membranes and torovirus infection. Although toroviruses were not observed in any sample, other viruses or virus-like particles were observed in faeces from one of 15 cats with the syndrome, six of 21 cats which had experienced prior episodes of the syndrome and four of 15 healthy control cats. The findings of this study do not support the hypothesis that the syndrome is associated with torovirus infection. 相似文献
69.
水稻试验小区边际效应的估算 总被引:8,自引:0,他引:8
本文对水稻试验小区各行实际产量与行的对应关系进行曲线拟合,从而获得可用来评估小区边际效应的模拟曲线方程,根据方程提供的参数δ可进行小区边际效应的评估。如在产量潜力评估的
各级试验中加以采用,将极大地提高试验结果的准确性。 相似文献
70.
水稻大面积高产综合配套技术研究开发与示范1996年度课题攻关报告 总被引:1,自引:0,他引:1
湖南省课题攻关协作组 《中国水稻科学》1998,12(Z1):1-3
总结了1996年水稻大面积高产综合配套技术研究开发与示范的进展。 相似文献