Diagnostic and public health investigation of Mycobacterium tuberculosis infection in a dog in Ontario,Canada     

Luke A. J. Haydock  Anthony C. G. Abrams-Ogg  J. Scott Weese  Michael R. Goldstein  Amy B. Clifford  Adrian Sebastian  Elizabeth H. Rea  Frances B. Jamieson  Carla Duncan  Olga Andrievskaia  Mirjana Savic  Durda Slavic  Robert A. Foster  Christopher J. Greenwood  Tamara L. MacDonald  Jacqueline E. Scott  Andrea Sanchez 《Journal of veterinary diagnostic investigation》2022,34(2):292

A 4-y-old, female mixed-breed dog was presented to the Ontario Veterinary College for further evaluation of multiple pulmonary and hepatic masses, intrathoracic lymphadenitis, and recent development of a pyogranulomatous pleural effusion. Along with other comprehensive tests, a thoracic lymph node biopsy was performed, and Mycobacterium tuberculosis complex infection was confirmed by real-time PCR. The dog’s condition declined post-operatively, and euthanasia was elected. Postmortem examination confirmed severe granulomatous pneumonia, hepatitis, intrathoracic and intraabdominal lymphadenitis, omentitis, and nephritis. Line-probe assays performed on samples collected postmortem confirmed the species as M. tuberculosis. 24-loci MIRU-VNTR genotyping, spoligotyping, and whole-genome sequencing revealed relations to known human isolates, but no epidemiologic link to these cases was investigated. Given the concern for potential human exposure during this animal’s disease course, a public health investigation was initiated; 45 individuals were tested for M. tuberculosis exposure, and no subsequent human infections related to this animal were identified. Our case highlights the need for more readily available, minimally invasive testing for the diagnosis of canine mycobacteriosis, and highlights the ability of canid species to act as potential contributors to the epidemiology of M. tuberculosis infections.  相似文献   

Improving Drought Tolerance of Rice by Designed QTL Pyramiding   总被引：4，自引：0，他引：4  

Drought is the most important factor limiting rice yields in the rainfed areas of Asia. To overcome the problem  we developed a new strategy ''''designed QTL pyramiding'''' to more efficiently develop drought tolerant 《分子植物育种》2007,5(2):205-206

Drought is the most important factor limiting rice yields in the rainfed areas of Asia. To overcome the problem, we developed a new strategy ＇designed QTL pyramiding＇ to more efficiently develop drought tolerant （DT） rice cultivars. In the first phase of this strategy, we developed large numbers of introgression lines （ILs） in elite backgrounds using backcross （BC） breeding, each of which carries multiple genomic segments for improved DT from a known donor. Then, we genotyped all ILs with SSR markers to track the genomewide pattern of introgression in the DT ILs of 16 BC populations derived from crosses between 2 recipients （IR64 and Teqing） and four different donors. X^2 tests and linkage disequilibrium analyses of introgression in the DT ILs revealed significant frequency shifts at many loci across the genome and non-random associations at the multilocus level as a result of selection for DT in the ILs, which led us to the discovery of putative genetic networks underlying DT in the ILs. The networks each containing all DT loci detected in ILs from a BC population showed some interesting features.  相似文献   

TILLING and Ecotilling the Plant Kingdom     

The modern crop scientist has a large amount of available nucleotide sequence information to identify genes of potential agronomic importance.…… 《分子植物育种》2007,5(2):238-239

The modem crop scientist has a large amount of available nucleotide sequence information to identify genes of potential agronomic importance. Using reverse genetic approaches, specific genes can be disrupted, and hypotheses regarding gene function directly tested in vivo. Although a number of reverse genetic methods have been introduced, many are limited in application because they are organism-specific, expensive, transgenic, or only transiently disrupt gene function. However, traditional mutagenesis using chemical mutagens has been widely used as a forward genetics strategy to create many new crop plant varieties at relatively low cost. Mutagens such as ethyl methanesulphonate （EMS） cause stable point mutations and thus produce an allelic series of truncation and missense changes that can provide a range of phenotypes （Greene et al., 2003）. TILLING （targeting induced local lesions IN genomes） is a high-throughput reverse genetic strategy that combines traditional mutagenesis and SNP discovery methods （Colbert et al., 2001; McCallum et al., 2000）. To identify mutations, target regions of-l.5 kb are amplified with fluorescently labeled gene specific primers. Heteroduplexes are then formed between wild-type and mutant strands, mismatches are cleaved by incubation with a single-strand specific nuclease,  相似文献   

Mapping QTLs for Yield Stability in Durum Wheat Grown under Different Water Regimes     

Among the most important Mediterranean annual crops  durum wheat is widely grown in drought-prone areas. Therefore  improving water-use efficiency 《分子植物育种》2007,5(2):223-223

Among the most important Mediterranean annual crops, durum wheat is widely grown in drought-prone areas. Therefore, improving water-use efficiency （WUE） of durum wheat represents a major breeding goal. IDu-WUE （Improving Durum wheat for Water Use Efficiency and yield stability through physiological and molecular approaches） is a collaborative project among public and private research centres in Italy, Spain and WANA （West Asia and North Africa） countries （Morocco, Tunisia, Syria and Lebanon） funded by the European Union aimed at investigating the genetic variation for WUE and yield stability in durum wheat grown in Mediterranean droughtprone areas. During the first year of the project, a number of morpho-physiological traits （e.g. early vigour, flowering time, leaf rolling, number of fertile tillers, etc.）, WUE, WUE-related traits （e.g. carbon isotope discrimination, canopy temperature, chlorophyll fluorescence, etc.）, yield and its components have been investigated in a RIL population （249 lines） and a collection of ca. 190 durum wheat accessions characterized by a high level of linkage disequilibrium （Maccaferri et al., 2005）,  相似文献   

华南地区覆盖旱种水稻节水及其水分利用效率研究   总被引：3，自引：0，他引：3  

汪强  樊小林  D.Klaus  B.Sattemacher 《灌溉排水学报》2007,26(4):89-92

大田试验条件下设置常规水稻(T11)、覆膜旱作水稻(T12)、覆草旱作水稻(T13)和裸地旱作水稻(T14)4个处理,研究旱作水稻的节水效率及水分利用效率。水稻本田期T11用水总量545.40 mm,显著高于各旱作处理水稻,以此为参照,T12、T13和T14节水效率分别为35.1%、22.8%和21.2%。以裸地旱作处理T14为参照,T12节水率17.7%,而T13无明显节水效果。旱作水稻的节水效果主要发生在水稻移栽后前2个月,之后无明显节水效果。各处理以籽粒产量/耗水量为基础计算的水分利用效率(WUE),T12、T13、T14和T11处理分别为1.47、1.31、1.21和1.05 g/kg,以干物质量/耗水量为基础计算的WUE各处理之间的趋势与以籽粒产量/耗水量计算的WUE基本一致。  相似文献   

Investigation of the distance to slaughterhouses and weather parameters in the occurrence of porcine reproductive and respiratory syndrome outbreaks in U.S. swine breeding herds     

Justin Moeller  Jerry Mount  Emily Geary  Magnus R. Campler  Cesar A. Corzo  Robert B. Morrison  Andria G. Arruda 《The Canadian veterinary journal. La revue veterinaire canadienne》2022,63(5):528

Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically important diseases affecting the swine industry. The main objective of this study was to assess whether sow farm distance to slaughterhouses and meteorological variables were associated with PRRS outbreaks. This case-control study paired 104 sow farms with or without a reported PRRS outbreak (N = 208) during the same period. Data on the distance to the closest slaughterhouse, swine density, presence of an air filtration system, or a neighboring farm, and weather conditions were collected, and a multivariable conditional logistic regression model was created to investigate the association between variables of interest and the occurrence of a PRRS outbreak.Swine density, presence of an air filtration system, presence of a neighboring farm, and PRRS herd-level status before the outbreak were associated with the occurrence of PRRS outbreaks. Farms in areas with higher swine density and nearby swine farms had increased odds of reporting an outbreak compared to farms in low swine density areas and farms having no neighbors. Under the conditions of this study, none of the meteorological variables or the distance to the closest slaughterhouse were associated with occurrence of PRRS outbreaks.  相似文献   

植物根系对土壤水力参数影响的定量研究综述     

鲁建荣  李云良  谭志强  姬明飞  杨垒  B. Larry Li 《土壤学报》2023,60(4):939-952

植物根系是土壤结构以及土壤水力参数变化的重要影响要素。目前不仅缺乏定量描述“根-孔隙-土壤水力参数”相互作用的研究方法,在更大尺度上根系作用的客观表达也尚不明确,由此导致降雨入渗、径流和蒸发等流域水文过程的精细刻画与模拟预测具有很大的不确定性。基于文献检索,本文对国内外相关研究进行了回顾与梳理,量化了植物根系对土壤水力参数的改变和影响,并提出其与植被、土壤类型的响应方式,总结了植物根系动态性生长下的土壤水力参数定量表述及其预测模型进展。同时分析了在定量研究根-土复合系统中存在的问题及未来研究的发展方向,指出目前根系影响土壤水力参数的研究主要集中在小尺度控制实验方面,忽略了大尺度下土壤空间异质性及外部环境因素的干扰,强调大尺度根系作用和根系参数纳入土壤结构的重要性和实际意义,进一步与水文模型的深度耦合逐渐成为未来研究的热点。  相似文献   

Soil microbial communities resistant to changes in plant functional group composition     

Carolyn B. Marshall  Roy Turkington 《Soil biology & biochemistry》2011,43(1):78-85

The soil community is an often ignored part of research which links plant biodiversity and ecosystem functioning despite their influence on numerous functions such as decomposition and nutrient cycling. Few consistent patterns have been detected that link plant and soil community composition. We used a removal experiment in a northern Canadian grassland to examine the effects of plant functional group identity on soil microbial community structure and function. Plant functional groups (graminoids, legumes and forbs) were removed independently from plots for five growing seasons (2003-2007) and in the fifth year effects on the soil microbial community were examined using substrate-induced respiration (SIR - a measure of metabolic diversity) and phospholipid fatty acid analysis (PLFA - a measure of microbial community composition). Removal treatments were also crossed with both a fertilizer treatment and a fungicide treatment to determine if effects of functional group identity on the soil community were context dependent. Plant functional group identity had almost no effect on the soil microbial community as measured by either SIR or PLFA. Likewise, soil properties including total carbon, pH, moisture and nutrients showed a limited response to plant removals in the fifth year after removals. We found a direct effect of fertilizer on the soil community, with fertilized plots having decreased metabolic diversity, with a decreased ability to metabolize amino acids and a phenolic acid, but there was no direct soil microbial response to fungicide. We show that in this northern Canadian grassland the soil microbial community is relatively insensitive to changes in plant functional group composition, and suggest that in northern ecosystems, where plant material is only slowly incorporated into the soil, five growing seasons may be insufficient to detect the impact of a changing plant community on the soil microbes.  相似文献   

Land-use history has a stronger impact on soil microbial community composition than aboveground vegetation and soil properties     

Kamlesh Jangid  Mark A. Williams  Thomas M. Schmidt  William B. Whitman 《Soil biology & biochemistry》2011,43(10):2184-2193

The response of soil microbial communities following changes in land-use is governed by multiple factors. The objectives of this study were to investigate (i) whether soil microbial communities track the changes in aboveground vegetation during succession; and (ii) whether microbial communities return to their native state over time. Two successional gradients with different vegetation were studied at the W. K. Kellogg Biological Station, Michigan. The first gradient comprised a conventionally tilled cropland (CT), mid-succession forest (SF) abandoned from cultivation prior to 1951, and native deciduous forest (DF). The second gradient comprised the CT cropland, early-succession grassland (ES) restored in 1989, and long-term mowed grassland (MG). With succession, the total microbial PLFAs and soil microbial biomass C consistently increased in both gradients. While bacterial rRNA gene diversity remained unchanged, the abundance and composition of many bacterial phyla changed significantly. Moreover, microbial communities in the relatively pristine DF and MG soils were very similar despite major differences in soil properties and vegetation. After >50 years of succession, and despite different vegetation, microbial communities in SF were more similar to those in mature DF than in CT. In contrast, even after 17 years of succession, microbial communities in ES were more similar to CT than endpoint MG despite very different vegetation between CT and ES. This result suggested a lasting impact of cultivation history on the soil microbial community. With conversion of deciduous to conifer forest (CF), there was a significant change in multiple soil properties that correlated with changes in microbial biomass, rRNA gene diversity and community composition. In conclusion, history of land-use was a stronger determinant of the composition of microbial communities than vegetation and soil properties. Further, microbial communities in disturbed soils apparently return to their native state with time.  相似文献   

Insular pest control within a metapopulation context     

James C. Russell  Jamie W.B. Mackay  Jawad Abdelkrim 《Biological conservation》2009,142(7):1404-1410

Introduced pests threaten many species and their control is generally beneficial for conservation, particularly on islands where complete eradication is possible. Unfortunately on ‘nearshore’ islands neighbouring source populations exist and unaided reinvasion is likely. Pest control programmes at these sites thus require a metapopulation context to adequately manage movements between source and sink populations. We investigated the ecology of introduced ship rats (Rattus rattus) on a nearshore island, and gene flow with adjacent mainland populations, in order to understand the metapopulation dynamics and relative levels of pest control required within the landscape. We sampled the entire population by trapping (n = 30), achieving eradication, and found a low rat density (3.2 ha⁻¹) indicative of a sink population. Seed and other plant material constituted the major dietary component of rats. Despite its proximity to mainland source populations, the island population was genetically distinct with reduced allelic diversity caused by a recent reinvasion founder effect. Genetic analyses also detected recent migrants between the populations. In contrast, two mainland populations separated by a similar distance displayed complete genetic mixing. The small water gap therefore provides a sufficient barrier to lower the migration rate to the island and delay reinvasion, which nonetheless eventually happens. In order to maintain nearshore islands pest-free, conservation management will require a metapopulation approach simultaneously focusing on both island and source population pest control.  相似文献