Enzootic bovine leukosis virus in Brazil     

Carlos H. Romero  Cheryl A. Rowe 《Tropical animal health and production》1981,13(1):107-111

Summary A sero-epidemiological survey for antibodies to the glycoprotein of enzootic bovine leukosis virus showed that the infection is widely disseminated in the State of Rio de Janeiro, Brazil. Sera from 1,290 females and 154 males from 12 dairy herds were tested by the agar gel precipitin test. Seven hundred and one females (54.3%) and 68 males (44.2%) were found to have specific antibodies. These antibodies were demonstrated in all 7 age groups tested. The older age groups contained the highest percentage of reactors. The results are briefly discussed in relation to management practices and environmental conditions.

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El Virus De La Leucosis Bovina Enzootica En Brasil

Resumen Una encuesta suero-epidemiológica por anticuerpos contra la glicoproteina del virus de la leucosis bovina enzootica mostró que la infección está ampliamente diseminada en el Estado de Rio de Janeiro, Brasil. Sueros de 1290 hembras y 154 machos pertenecientes a 12 rebaños lecheros fueron examinados en la prueba de precipitación en agar. Se encontró que 701 hembras (54.3 por ciento) y 68 machos (44.2 por ciento) poseían anticuerpos específicos. Estos anticuerpos fueron demonstrados en todos los siete grupos de edad examinados. Los grupos de mas edad contenian un porcentaje mayor de reactores. Los resultados son discutidos brevemente en relación a las prácticas de manejo y a las condiciones ambientales locales.

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Virus De La Leucose Bovine Enzootique Au Bresil

Résumé Une enquête séro-épidémiologique pour la détection de la présence de la glycoproteine du virus de la leucose enzootique bovine a montré que cette affection est largement disséminée dans l'Etat de Rio de Janeiro, au Brésil. Des échantillons de sérum provenant de 1290 femelles et 154 mâles appartenant à 12 troupeaux laitiers ont été étudiés par précipitation en gélose—701 femelles (54,3 p. 100) et 68 mâles (44,2 p. 100) ont été reconnus comme possédant des anticorps spécifiques. Ces anticorps ont été mis en évidence dans la totalité des 7 groupes d'âges étudiés. Les groupes d'âges plus élevés contenaient le plus haut pourcentage d'animaux positifs. Les résultats sont brièvement discutés, dans leur relation avec les pratiques de l'élevage et les conditions de l'environnement.

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Use of goose red blood cells for detection of infection with psittacine beak and feather disease virus by haemagglutination and haemagglutination inhibition     

N SEXTON  WJ PENHALE  SL PLANT  DA PASS 《Australian veterinary journal》1994,71(10):345-347

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Reply     

DA SHUTT  LR FELL 《Australian veterinary journal》1988,65(12):404-404

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海西东部及环青海湖地区40多年的气候变化研究   总被引：11，自引：3，他引：8  

时兴合  李凤霞  扎西才让  达成荣  贾红莉  傅生武 《干旱地区农业研究》2005,23(2):215-222

利用1961～2004年地面观测资料,分析了海西东部及环青海湖地区年、季气候变化的特征和规律。结果表明,海西东部及环青海湖40多年来年季平均气温平均增温幅度为0．3～0．6℃,平均最低和最高气温多数季节增温具有非对称性。并且20世纪90年代四季增暖最为明显。夏季和冬季降水略有增加,而过渡季节降水变化基本呈减少趋势。年均和秋、冬、春、夏四季的气温变化倾向率均为正值．分别为0．48、0．57、0．692、0．337、0．373℃／10a。冬、夏季降水变化的倾向率为0．442、4．672mm／10a;而秋、春季了水变化的倾向率为-2．304、-0．022mm／10a。冬季降水量和累计积雪量的缓慢增加容易导致该地区地表积雪的积累,极易形成雪灾或低温冻害。春季气温升高、降水减少。干旱发生的频次将增加;秋、冬季气温上升幅度比较大．将有利于病虫害的越冬、繁殖,加重病虫害对农牧业生产的危害程度。  相似文献   

Investigations of an enteric infection of cockatoos caused by an enterovirus-like agent     

SL. WYLIE  DA. PASS 《Australian veterinary journal》1989,66(10):321-324

An enteric infection in cockatoos associated with a 30 nm diameter enterovirus-like agent seen in faeces and intestinal epithelial cells is described. The disease is characterised by intractable, profuse, mucoid diarrhoea, weight loss, dehydration and death. Lesions in the intestine consist of villous atrophy, villous fusion, enterocyte hyperplasia and, in some cases, chronic inflammation. Affected birds so far examined have concurrent psittacine beak and feather disease.  相似文献   

Experimental transmission of a herpesvirus in Greek tortoises (Testudo graeca)     

Origgi FC  Romero CH  Bloom DC  Klein PA  Gaskin JM  Tucker SJ  Jacobson ER 《Veterinary pathology》2004,41(1):50-61

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Geminivirus C2 protein represses genes involved in sulphur assimilation and this effect can be counteracted by jasmonate treatment     

Rosa Lozano-Durán  Irene García  Stéphanie Huguet  Sandrine Balzergue  Luis C. Romero  Eduardo R. Bejarano 《European journal of plant pathology / European Foundation for Plant Pathology》2012,134(1):49-59

Geminiviruses are plant viruses that infect a broad range of crops and cause extensive losses worldwide, having an important economic impact. C2, a multifunctional pathogenicity factor encoded by geminiviruses, has been recently shown to suppress the responses to jasmonates in the host plant, which might at least partially explain its well-established role in pathogenicity. Sulphur is one of the essential macro-elements for plant life, and is considered to have a role in plant defence, in a phenomenon named sulphur-induced resistance (SIR) or sulphur-enhanced defence (SED). In this work, we show that geminivirus C2 protein represses the expression of genes involved in the sulphur assimilation pathway in Arabidopsis, but, interestingly, this effect can be neutralized by exogenous jasmonate treatment. These preliminary results may raise the idea that geminiviruses might be affecting sulphur metabolism, and maybe counteracting SIR/SED, through the manipulation of the jasmonate signalling pathway, which would define a novel strategy in plant-virus interactions and may unveil SIR/SED as an important player in the plant defence against viruses.  相似文献   

农副产品及废弃物化感抑藻作用的研究与机理   总被引：1，自引：0，他引：1  

边归国  赵卫东  达来 《内蒙古农业大学学报(自然科学版)》2012,33(2):268-274

农副产品废弃物作为可再生性、可降解性的生物质资源,在农村被大量的丢弃和焚烧,给环境造成了极大地危害.通过一系列技术处理,可以从农副产品和废弃物中提取化感物质,应用于海水和淡水中藻类的抑制,以有效利用废物资源.文章对化感作用的概念、20种农副产品及废弃物对19种藻类的化感抑制作用以及化感作用抑藻机理等进行了较系统的论述,对今后植物化感作用抑藻技术的研究方向进行了展望.提出应多学科结合,利用化感物质对不同藻类的影响及其选择性抑制机理做更深入的研究.  相似文献   

Evaluation of two cocktails containing ESAT-6, CFP-10 and Rv-3615c in the intradermal test and the interferon-γ assay for diagnosis of bovine tuberculosis     

Casal C  Bezos J  Díez-Guerrier A  Álvarez J  Romero B  de Juan L  Rodriguez-Campos S  Vordermeier M  Whelan A  Hewinson RG  Mateos A  Domínguez L  Aranaz A 《Preventive veterinary medicine》2012,105(1-2):149-154

The intradermal tuberculin tests and the interferon-gamma (IFN-γ) assay are the principal tests used worldwide for the ante-mortem diagnosis of bovine tuberculosis. The conventional reagent currently in use in these tests is purified protein derivative (PPD) tuberculin obtained from Mycobacterium bovis culture. The components of PPD are poorly characterized and difficult to standardize. To overcome this issue, antigens specific to the Mycobacterium tuberculosis complex are being studied. Here we have assessed the biological potency of ESAT-6, CFP-10 and Rv-3615c presented as peptide or recombinant protein cocktails in comparison with the standard bovine PPD used routinely in Spanish eradication campaigns. The study was performed in cattle (n=23) from a herd with natural M. bovis infection. Animals were simultaneously injected with PPD and the peptide and protein cocktails. The percentages of cattle reacting positively to single intradermal test were 60.9% (bovine PPD), 47.8% (peptide cocktail) and 60.9% (protein cocktail), with no significant difference between the actual skin fold thickness increases (p>0.05). The IFN-γ assay detected 60.9% of animals when stimulation was performed with bovine PPD, but decreased to 52.2% when stimulation was performed with the peptide cocktail and to 47.8% when stimulation was performed with the protein cocktail. However, no significant differences were found between IFN-γ responder frequencies (p>0.05). These results show a potential use of these defined reagents for in vivo tuberculosis diagnosis.  相似文献   

Comparative study of equine bone marrow and adipose tissue-derived mesenchymal stromal cells     

Ranera B  Ordovás L  Lyahyai J  Bernal ML  Fernandes F  Remacha AR  Romero A  Vázquez FJ  Osta R  Cons C  Varona L  Zaragoza P  Martín-Burriel I  Rodellar C 《Equine veterinary journal》2012,44(1):33-42

Reasons for performing study: Mesenchymal stromal cells (MSCs) represent an attractive source for regenerative medicine. However, prior to their application, fundamental questions regarding molecular characterisation, growth and differentiation of MSCs must be resolved. Objectives: To compare and better understand the behaviour of equine MSCs obtained from bone marrow (BM) and adipose tissue (AT) in culture. Methods: Five horses were included in this study. Proliferation rate was measured using MTT assay and cell viability; apoptosis, necrosis and late apoptosis and necrosis were evaluated by flow cytometry. The mRNA expression levels of 7 surface marker genes were quantified using RT‐qPCR and CD90 was also analysed by flow cytometry. Differentiation was evaluated using specific staining, measurement of alkaline phosphatase activity and analysis of the mRNA expression. Results: High interindividual differences were observed in proliferation in both cell types, particularly during the final days. Statistically significant differences in viability and early apoptosis of cultured AT‐ and BM‐MSCs were found. The highest values of early apoptosis were observed during the first days of culture, while the highest percentage of necrosis and late apoptosis and lowest viability was observed in the last days. Surface marker expression pattern observed is in accordance to other studies in horse and other species. Osteogenic differentiation was evident after 7 days, with an increasing of ALP activity and mRNA expression of osteogenic markers. Adipogenic differentiation was achieved in BM‐MSCs from 2 donors with one of the 16 media tested. Chondrogenic differentiation was also observed. Conclusions: Proliferation ability is different in AT‐MSCs and BM‐MSCs. Differences in viability and early apoptosis were observed between both sources and CD34 was only found in AT‐MSCs. Differences in their osteogenic and adipogenic potential were detected by staining and quantification of specific tissue markers. Potential relevance: To provide data to better understand AT‐MSCs and BM‐MSCs behaviour in vitro.  相似文献