"Dip-Pen" nanolithography   总被引：1，自引：0，他引：1  

RD Piner  J Zhu  F Xu  S Hong  CA Mirkin 《Science (New York, N.Y.)》1999,283(5402):661-663

A direct-write "dip-pen" nanolithography (DPN) has been developed to deliver collections of molecules in a positive printing mode. An atomic force microscope (AFM) tip is used to write alkanethiols with 30-nanometer linewidth resolution on a gold thin film in a manner analogous to that of a dip pen. Molecules are delivered from the AFM tip to a solid substrate of interest via capillary transport, making DPN a potentially useful tool for creating and functionalizing nanoscale devices.  相似文献   

Self-assembling protein microarrays     

Ramachandran N  Hainsworth E  Bhullar B  Eisenstein S  Rosen B  Lau AY  Walter JC  LaBaer J 《Science (New York, N.Y.)》2004,305(5680):86-90

Protein microarrays provide a powerful tool for the study of protein function. However, they are not widely used, in part because of the challenges in producing proteins to spot on the arrays. We generated protein microarrays by printing complementary DNAs onto glass slides and then translating target proteins with mammalian reticulocyte lysate. Epitope tags fused to the proteins allowed them to be immobilized in situ. This obviated the need to purify proteins, avoided protein stability problems during storage, and captured sufficient protein for functional studies. We used the technology to map pairwise interactions among 29 human DNA replication initiation proteins, recapitulate the regulation of Cdt1 binding to select replication proteins, and map its geminin-binding domain.  相似文献   

Response of russet norkotah clonal selections to nitrogen fertilization   总被引：1，自引：0，他引：1  

Francis Zvomuya  Carl J. Rosen  J. Creighton Miller 《American Journal of Potato Research》2002,79(4):231-239

The low vine vigor and high N requirement of Russet Norkotah may lead to N loss and groundwater contamination on coarse-textured soils. Recent clonal selections from Texas have produced strains that have larger and stronger vines, which may alter N requirements. This twoyear study examined the N use efficiency (NUE), yield, and quality of Russet Norkotah strain selections fertilized with different N levels on a Hubbard loamy sand in central Minnesota. The selections, Texas Norkotah Strain (TXNS) 112, TXNS 223, and TXNS 278 were grown with standard Russet Norkotah under irrigated conditions and received total N levels of 28, 112, 224, or 336 kg ha^-1. Total, marketable, and large (>340 g) tuber yields increased linearly (P>0.05) with rate of N application in 1998 but not in 1997. The genotype main effect was not significant for any of the tuber yield parameters measured based on fresh weight. Vine, tuber, and total dry biomass yields were 116%, 5.8%, and 13.2%, respectively, higher with the selections than Russet Norkotah. Harvest index (HI), or the proportion of total dry matter partitioned to tubers, was 7% greater for Russet Norkotah than the TXNS selections, reflecting the larger vine growth of the selections. The selections accumulated significantly higher N in the vines (0.113 kg kg^-1 N) than the standard clone (0.053 kg kg^-1 N) as N rate increased from 28 to 336 kg ha^-1, and the difference between the selections and the standard clone was larger at higher N rates than at lower N rates. Russet Norkotah partitioned 10% more N to tubers than did the TXNS selections, reflecting the difference in HI between the standard cultivar and its clones. Nitrogen recovered from fertilizer N applied in addition to the 28 kg ha^-1 starter N (NUE₂₈) averaged 36% and varied little with genotype, N rate, or cropping year. Biomass accumulation from similar N additions (AUE), however, was significantly higher for the selections than Russet Norkotah at 112 kg N ha^-1 in 1997 only. At low N rate (112 kg ha^-1), the selections had higher physiological use efficiency (PUE₂₈) (mean 45.9 g g¹) than Russet Norkotah (25.9 g g¹). Results from this study demonstrate that, although N recovery was similar for the four genotypes, the Texas Norkotah strains produced greater biomass than Russet Norkotah per kg N applied at low rate in 1997 and per kg of fertilizer N absorbed by the plant in both years. However, under the conditions of this study, higher biomass of the selections did not translate into a marketable yield advantage over the standard cultivar.  相似文献   

Effect of pre-medication on gastroduodenoscopy in isoflurane-anesthetized cats     

AA  Smith  LP Posner  RE Goldstein  JW Ludders  HN Erb  KW Simpson  RD Gleed 《Veterinary anaesthesia and analgesia》2003,30(2):103-103

The pre‐medicant chosen may influence the ease with which gastroduodenoscopy (GD) is performed. The purpose of this study was to evaluate the relative ease of GD in cats under ketamine and isoflurane anesthesia after IM injection of hydromorphone (H, 0.1 mg kg^?1), hydromorphone plus glycopyrrolate (HG, 0.1 mg kg^?1 (H), 0.01 mg kg^?1 (G)), medetomidine (M, 0.03 mg kg^?1), or butorphanol (B, 0.4 mg kg^?1). Eight cats were assigned randomly to receive each treatment in a cross‐over design with at least 7 days between treatments. Twenty minutes after pre‐medication, medetomidine produced greater (p = 0.001) sedation than the other treatments when assessed, using a subjective ordinal scale. The cats were injected with ketamine (10 mg kg^?1 IM), orotracheally intubated, connected to a pediatric circle breathing system, and allowed to spontaneously breathe isoflurane in oxygen. Once end‐tidal isoflurane concentration was stable at 1.4% for 15 minutes, endoscopy was started. A single endoscopist (REG), who was unaware of the treatment used, performed all endoscopies. The endoscopist scored the difficulty of endoscopy subjectively (0–3). The significance of differences between treatments was evaluated using Friedman's test. Time for entering the stomach was 9.4 (4.7–15.9) (median (minimum–maximum)), 6.6 (5.2–11.7), 8.4 (6.3–16.5), and 7.7 (5.1–14.7) seconds and for entering the duodenum from the stomach was 20.5 (13.8–40.9), 18.2 (10.3–39.8), 20.2 (16.2–119.5), and 22.2 (11.8–83.8) seconds for H, HG, M, and B treatments, respectively. There were no significant differences in the time for, or difficulty of, endoscopy. We conclude that any of these drugs can be used satisfactorily at the doses and combinations tested to pre‐medicate cats prior to general anesthesia for GD.  相似文献   

A national programme for mastitis control in Australia: Countdown Downunder     

PB Brightling  RD Dyson  AF Hope  J Penry 《Irish veterinary journal》2009,62(Z4):S52

In 1998, Countdown Downunder, Australia's national mastitis and cell count control programme, was created. With funding from the country's leading dairy organisation, Dairy Australia, this programme was originally intended to run for three years but is now in its tenth year. As it was the first time Australia had attempted a national approach to mastitis control on the farm, the first three years of the programme were largely concerned with the development of resources to be used by farmers and service providers. The second three years were devoted to training with both groups. Since that time, Countdown Downunder has entered into a second resource development phase. The goal of the programme was to achieve a reduction in the bulk milk somatic cell count from the Australian dairy herd. To achieve this, the programme had to develop resources with clear and consistent messages around mastitis and somatic cell count control on farms. It was determined that progress toward the goals would be made more rapidly if service providers were trained in the use of these resources prior to farmers. This paper reviews the Countdown Downunder programme from 1998 to 2007.  相似文献   

Nutritional responses in rainbow trout (Oncorhynchus mykiss) fed diets with different physical qualities at stable or variable environmental conditions     

T.S. AAS  B.F. TERJESEN  T. SIGHOLT  M. HILLESTAD  J. HOLM  S. REFSTIE  G. BAEVERFJORD  K.‐A. RØRVIK  M. SØRENSEN  M. OEHME  T. ÅSGÅRD 《Aquaculture Nutrition》2011,17(6):657-670

Rainbow trout with initial body weight 1144 g were fed two diets with high (feed A) or low (feed B) water stability for 6 weeks. During the last 2 weeks either stable or fluctuating oxygen saturation, salinity and temperature was introduced. High water stability of the feed was associated with harder pellets, less dust formation and less broken pellets compared to feed with low water stability. During the first 4 weeks, feed intake was 23% higher in trout fed diet B than in those fed diet A. Fluctuating environment resulted in a severe drop in feed intake for both dietary groups. At termination of the trial, stomachs of trout fed feed B contained mashed pellets and free water and oil. Stomachs of trout fed feed A contained more intact pellets and little fluid. The apparent digestibility of protein, starch, dry matter and energy was highest in feed A. The apparent digestibility of P and Zn was highest at stable environment, and the absorption of P was highest from feed A. In conclusion, physical quality affected the nutritional value of the feeds, and differences in nutritional value at stable or fluctuating environment appeared to be related to feed intake.  相似文献