Distribution of Ground Water Suitable for Use in Saline-Water Aquaculture in Central and West-Central Alabama     

Christopher A. Boyd  Phillip L. Chaney  Claude E. Boyd  David B. Rouse 《Journal Of Applied Aquaculture》2013,25(4):228-240

Production of shrimp in inland ponds supplied by water from saline aquifers is a potential new aquaculture industry in Alabama. Examination of 2,527 well records of the Geological Survey of Alabama and the United States Geological Survey and samples from 35 wells revealed 238 wells with chloride concentration of 125 mg/L chloride or more in 11 counties of central and west-central Alabama. Chloride concentrations varied from 136 mg/L to 94,000 mg/L. The highest chloride concentrations were from wells in Washington, Choctaw, and Clarke Counties. However, 83% of the saline-water wells in Dallas, Hale, Greene, Marengo, Wilcox, and Sumter Counties were supplied by aquifers of the Eutaw, Gordo, McShan, and undifferentiated Eutaw-McShan formations. Water from these wells had an average and standard deviation of 1,238 ± 615 mg/L chloride. Based on the frequency of saline-water wells, the number of wells with chloride concentrations above 700 mg/L (about 2 ppt salinity), and well depths, it was concluded that Hale, Greene, Marengo, and Sumter Counties have the greatest potential for saline-water aquaculture. Contour maps for chloride concentrations and depths of wells in these four counties were prepared to better delineate this potential water source for aquaculture. There were, however, areas in Lowndes, Tuscaloosa, and Wilcox Counties with saline ground water suitable for inland aquaculture.  相似文献   

Comparison of a competitive inhibition ELISA and the card agglutination test for detection of antibodies to Anaplasma marginale and Anaplasma centrale in cattle     

JB MOLLOY  PM BOWLES  DP KNOWLES  TF McELWAIN  RE BOCK  TG KINGSTON  GW BLIGHT  RJ DALGLIESH 《Australian veterinary journal》1999,77(4):245-249

OBJECTIVE: To compare a recently developed recombinant MSP-5 competitive inhibition ELISA with a card agglutination test for detection of antibodies to Anaplasma marginale and Anaplasma centrale in Australian cattle. MATERIALS AND METHODS: The ELISA was compared with the card agglutination test using 208 sera from cattle in Anaplasma-free herds, 86 sera from cattle experimentally infected with A marginale or A centrale and 757 sera from cattle in areas endemic for A marginale. RESULTS: The specificity of the ELISA, based on testing 208 sera from cattle in Anaplasma-free areas, was 99.5%, and the sensitivities for detection of antibodies to A marginale and A centrale in sera from the experimentally infected cattle were 98.0% and 100%, respectively. For the same sets of sera, the specificity of the card agglutination test was 98.6% and the sensitivities for detection of antibodies to A marginale and A centrale were 98.0% and 100%, respectively. For the 757 sera collected from cattle in areas endemic for A marginale, the agreement between the ELISA and the card agglutination test depended on the positive threshold selected for the ELISA. The maximum achievable agreement was 91.5% (kappa = 0.73; 95% confidence interval 0.66, 0.79). CONCLUSION: We conclude that the competitive inhibition ELISA is a useful alternative to the card agglutination test for detection of A marginale or A centrale infection in cattle. The assay should be particularly useful for epidemiological applications such as prevalence studies and control programs.  相似文献   

鸭传染性浆膜炎的防治措施   总被引：1，自引：0，他引：1  

王铁梅  孙桂琴  纪国文  张德顺 《中国兽药杂志》2007,41(7):59-60

鸭传染性浆膜炎是侵害雏鸭的一种慢性或急性败血性传染病，病原体为鸭疫里默氏杆菌，已经为危害养鸭业的主要传染病。采取免疫接种、鸭舍消毒、治疗等，控制鸭传染性浆膜炎的发生。  相似文献   

Fumigation and Fertilizer Nitrogen Source Effects on Potato Yield,Quality, and Early Dying     

Keith A. Kelling  Douglas I. Rouse  Phillip E. Speth 《American Journal of Potato Research》2017,94(5):481-489

Research has shown that while fumigation and use of ammonium N can both reduce the severity of verticillium wilt (Verticillium dahliae Kleb.) of potatoes (Solanum tuberosum L.), the use of the two practices together raises concerns over feeding the crop only ammonium N under reduced nitrification conditions. To assess the validity of this concern, we conducted two 3-year field split-plot experiments with both using metam sodium fumigant (none, fall or spring applied) as the main plot. For the first experiment, N source (134 kg N ha^?1 as ammonium sulfate, urea, or ammonium nitrate) was the split, whereas for the second trial in-season N rate (0, 67, 134, or 202 kg N ha^?1 all as ammonium sulfate) was the split. For both trials, in 2 of the 3 years, fumigation significantly increased tuber yield by an average of 9.9 Mg ha^?1 and decreased late-season verticillium severity ratings from 77 to 45%. In some years, fumigation also increased the proportion of U.S. No. 1 tubers and tubers >170 g. No differences in crop yield or quality were observed between the various N sources applied. This was true even on spring-fumigated areas with the highest rate of ammonium N applied. These experiments confirm that the choice between in-season potato N fertilizer should be based on factors such as potential for benefits or N losses, cost, and convenience of use rather than concern over an interaction between fumigation and ammonical N. While both fumigation and N rate reduced verticillium severity ratings in some years, the lack of interaction suggests these factors are functioning independently.  相似文献   

Partial and total replacement of fish meal with soybean meal and brewer's grains with yeast in practical diets for Australian red claw crayfish Cherax quadricarinatus     

Laura A. Muzinic  Kenneth R. Thompson  Aaron Morris  Carl D. Webster  David B. Rouse  Lukas Manomaitis 《Aquaculture (Amsterdam, Netherlands)》2004,230(1-4):359-376

Three feeding studies were conducted to evaluate the effects of total replacement of fish meal (FM) with a combination of soybean meal (SBM) and brewer's grains with yeast (BGY) in diets for two separate strains of juvenile Australian red claw crayfish. In Experiment 1, three practical diets were formulated to be isonitrogenous (40% protein) and isocaloric (4.0 kcal available energy/g diet) and contained either 25%, 10%, or 0% fish meal. Variable percentages of SBM (35%, 46.8%, and 79.8%, respectively) and BGY-35 (0%, 30%, and 5%, respectively) replaced the fish meal. In Experiments 2 and 3, four practical diets were formulated to be isonitrogenous (40% protein) and isocaloric (4 kcal available energy/g diet) containing 24% or 0% fish meal. Diet 1 contained 24% fish meal, 23% SBM, and 0% BGY-35. A variable percentage of SBM (56.75%, 47.75%, and 40.75%, respectively) and BGY-35 (10%, 20%, and 30%, respectively) replaced the fish meal in the remaining three diets.

In Experiment 1, after 8 weeks, juvenile red claw fed all three diets had no significant difference (P>0.05) in final weight, percentage weight gain, or survival, which averaged 7.90 g, 3848%, and 83%, respectively. In Experiment 2, after 8 weeks, juveniles fed all four diets had no significant difference in final weight, percentage weight gain, or specific growth rate which averaged 11.46 g, 977%, and 3.08%/day, respectively. Percentage survival was not significantly different among treatments and averaged 79%. In Experiment 3, after 8 weeks, juvenile red claw fed all four diets had no significant difference in final weight, percentage weight gain, or specific growth rate which averaged 16.22 g, 457%, and 2.34%/day, respectively. Percentage survival was not significantly different among treatments and averaged 98%.

These results indicate that fish meal and shrimp meal can be totally replaced with soybean meal and BGY in diets for juvenile red claw crayfish. This may allow for less expensive diets by red claw producers, which may increase profitability.  相似文献   

Methyl isothiocyanate volatilization from fields treated with metam‐sodium     

Ibrahim A M Saeed  Douglas I Rouse  John M Harkin 《Pest management science》2000,56(9):813-817

Emission of methyl isothiocyanate (MITC) from fields treated with metam‐sodium (sodium N‐methyldithiocarbamate) is a potential environmental and human safety hazard. Concentrations of MITC at three heights above four arable fields were measured following the application of metam‐sodium at a rate of 480 litre ha⁻¹ (166 kg AI ha⁻¹). Two of these fields were treated by injection into a center‐pivot irrigation system (chemigation), while in the other two fields the fumigant was applied through injection directly into the soil. Generally, higher MITC air concentrations were observed above chemigated than above injected fields. Maximum MITC air concentrations were 11.2 and 7.4 µg m⁻³ recorded 10 cm above ground 6–8 h following application and the minimum concentrations were 0.7 and 0.2 µg m⁻³ observed at 200 cm 30–35 h after application above chemigated and injected fields, respectively. The estimated MITC respiratory exposure a worker might encounter during the re‐entry period ranged between 1.37 and 0.03 mg day⁻¹ in chemigated fields and between 0.35 and 0.02 mg day⁻¹ in the injected fields. These results suggest that application of the fumigant through injection reduced MITC volatilization losses in comparison with the chemigation method, thus posing a relatively lower risk of exposure to MITC emissions. © 2000 Society of Chemical Industry  相似文献   

Temperature-sensitive wheat stem rust resistance gene Sr15 is effective against Puccinia graminis f. sp. tritici race TTKSK     

L. Gao  E. M. Babiker  I. C. Nava  J. Nirmala  Z. Bedo  L. Lang  S. Chao  S. Gale  Y. Jin  J. A. Anderson  U. Bansal  R. F. Park  M. N. Rouse  J. M. Bonman  H. Bariana 《Plant pathology》2019,68(1):143-151

The wheat stem rust fungus, Puccinia graminis f. sp. tritici (Pgt), race TTKSK and related races pose a serious threat to world wheat production. Knowing the effectiveness of wheat stem rust resistance (Sr) genes against Pgt race TTKSK is fundamental in mitigating this threat through resistance breeding. Sr15 was previously identified as being ineffective against Pgt race TTKSK. Here, multirace disease phenotyping data, linkage analyses, allelism testing and haplotype analyses are presented to support the conclusion that Sr15 is effective against Pgt race TTKSK. Resistance to race TTKSK was mapped to Sr15 in a biparental population. Thirty-two accessions with Sr15 displayed seedling resistance phenotypes against race TTKSK. However, these accessions were susceptible as seedlings at high temperatures (22–25 °C), consistent with previous reports that the interaction between avirulent Pgt isolates and Sr15 is temperature-sensitive. Markers STS638, wri4 and KASP_IWB30995 were found to predict the presence of Sr15, suggesting the utility of these assays for marker-assisted selection in breeding programmes. The effectiveness of Sr15 to specific Pgt races and temperatures makes it a less-desirable TTKSK-effective gene. Wheat lines assayed as resistant to race TTKSK at the seedling stage may possess Sr15 and breeders should be aware of the limitations of Sr15 for conferring stem rust resistance.  相似文献   

Leaf and stem seedling rust resistance in wheat cultivars grown in Croatia     

Valentina Spanic  Matthew N. Rouse  James A. Kolmer  James A. Anderson 《Euphytica》2015,203(2):437-448

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Detection of Mycobacterium avium subsp. paratuberculosis in skeletal muscle and blood of ewes from a sheep farm in New Zealand     

SL Smith  DM West  PR Wilson  GW de Lisle  MG Collett  C Heuer 《New Zealand veterinary journal》2013,61(5):240-243

Abstract

AIM: To determine whether viable Mycobacterium avium subsp. paratuberculosis (Map) is present in skeletal muscle and blood in ewes with and without Johne's disease confirmed histologically.

METHODS: A total of 51 mixed-aged ewes in poor body condition from a farm with a history of clinical Johne's disease were culled and examined at necropsy. BACTEC radiometric culture was performed on samples of skeletal muscle from the biceps femoris, mononuclear cells in peripheral blood (hereafter referred to as blood), and ileum. Histological sections and Ziehl-Neelsen (ZN)-stained impression smears of terminal ileum and mesenteric lymph nodes were examined. Ewes were defined as having confirmed Johne's disease if there was histopathological evidence typical of the disease within the ileum and adjacent lymph nodes.

RESULTS: Eighteen of 21 (86%) ewes with confirmed clinical Johne's disease were culture-positive for Map from sites peripheral to the alimentary tract, comprising 15 from skeletal muscle and 13 from blood. Five of 30 (17%) ewes that did not have Johne's disease were culture-positive, with four from skeletal muscle and one from blood. The likelihood that ewes with confirmed Johne's disease had systemic Map infection compared with ewes without was determined as OR=30 (95% CI=6.3–142.0; p<0.001).

CONCLUSION: The prevalence of Map infection of skeletal muscle and blood in ewes with confirmed Johne's disease was 71% and 62% respectively, and in unaffected ewes was 13% for muscle and 3% for blood.

CLINICAL RELEVANCE: Skeletal muscle and blood are potential sources of exposure of humans to Map, and the risk appears higher from sheep with Johne's disease.  相似文献   

Tuberculosis in a New Zealand (Hooker's) sea lion     

WD Roe  PJ Duignan  L Meynier  GW de Lisle  DV Cousins 《New Zealand veterinary journal》2013,61(1)

Extract

Tuberculosis has been described in seven species of pinnipeds. Based on host preference, phenotype and genetic and antigenic testing, the causal bacterium has been classified as a distinct species, Mycobacterium pinnipedii, within the M. tuberculosis complex. An adult male New Zealand sea lion (Phocarctos hookeri) was found dead on the Otago coastline in mid 2005. Gross necropsy revealed multiple caseous foci throughout the lungs, subcutaneous and thoracic lymph nodes, and mesenteric lymph nodes. Histopathological examination showed granulomatous lesions containing numerous intra-lesional acid fast organisms. The organism was confirmed as being a member of the M. tuberculosis complex, based on restriction enzyme analysis. Spoligotyping is pending. This is the first confirmed case of mycobacterial disease in a New Zealand sea lion.  相似文献