Transmission of Verticillium wilt resistance to tetraploid potato via unilateral sexual polyploidization     

K. E. Frost  S. H. Jansky  D. I. Rouse 《Euphytica》2006,149(3):281-287

Summary Verticillium wilt is a serious disease of potato and is caused by the soil-borne fungi Verticillium dahliae and V. albo-atrum. No major cultivar is resistant to this disease. Two diploid interspecific potato clones, C287 and C545, were previously identified with consistently high levels of Verticillium wilt resistance and are thought to have the same genotype for the loci that confer resistance to V. dahliae stem colonization. The purpose of this study was to determine whether resistance to V. dahliae stem colonization could be transferred to the tetraploid level in potato via unilateral sexual polyploidization (USP). Progenies in eight families obtained by crossing C287 and C545 to two tetraploid breeding clones, S438 and S440, and the cultivar ‘Atlantic’ were planted in a V. dahliae-infested field and a field without a significant V. dahliae infestation. Resistance was evaluated relative to C545 and C287. There were differences among families for stem colonization and yield. No differences between the mean stem colonization of C545 and C287 progeny were detected. Family differences due to the tetraploid parents indicate that they contributed resistance to the progeny. Stem colonization data from this experiment were consistent with the proposed complementary two-gene model for Verticillium wilt resistance in the diploid parents. Unilateral sexual polyploidization is an effective method for transferring V. dahliae stem colonization resistance to the tetraploid level.  相似文献   

Novel method for detecting bovine immunoglobulin G in dried porcine plasma as an indicator of bovine plasma contamination   总被引：2，自引：0，他引：2  

Newgard JR  Rouse GC  McVicker JK 《Journal of agricultural and food chemistry》2002,50(11):3094-3097

Current U.S. Food and Drug Administration regulations prohibit feeding of protein derived from mammalian tissue, excluding blood and blood products and any product that consists entirely of porcine or equine protein. A novel lateral flow immunoassay device has been developed that can quickly and qualitatively determine the presence of bovine immunoglobulin G (IgG), a major component in blood products, at very low levels (0.01% v/v). The device can be used to test for bovine IgG commingling in spray-dried porcine plasma used in the feed industry. Producers and consumers alike could use this device to verify product content at threshold levels.  相似文献   

In vitro and in vivo studies on the effects of flystrike dressings on larvae of Lucilia cuprina     

GW LEVOT  PB HUGHES 《Australian veterinary journal》1990,67(9):337-338

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A unique race of the wheat stem rust pathogen with virulence on Sr31 identified in Spain and reaction of wheat and durum cultivars to this race     

Pablo D. Olivera  Dolors Villegas  Carlos Cantero-Martínez  Les J. Szabo  Matthew N. Rouse  Douglas G. Luster  Radhika Bartaula  Marta S. Lopes  Yue Jin 《Plant pathology》2022,71(4):873-889

Wheat stem rust, caused by Puccinia graminis f. sp. tritici, is a re-emerging disease, posing a threat to wheat production. In Spain, stem rust has been rarely detected since the 1970s, but infection was observed in wheat fields in 2018. We analysed six stem rust samples collected in Rota, Cádiz province and one from Monteagudo del Castillo, Teruel province. All the samples from Rota were typed as race TKTTF, whereas the sample from Monteagudo del Castillo, collected in a wheat field adjacent to barberry bushes, was typed as race TKHBK. This race has a unique and significant virulence combination that includes virulence to Sr31, Sr33, Sr53 and Sr59, and is avirulent to Rusty, a durum line developed for universal susceptibility to the wheat stem rust pathogen. TKHBK is the first race outside the Ug99 race group with virulence to Sr31 and the first known race with virulence to Sr59. Genotyping studies indicate that race TKHBK does not belong to the Ug99 or TKTTF race groups and constitutes a previously unknown lineage. Two hundred bread and durum wheat cultivars and breeding lines from Spain were evaluated against TKHBK, TKTTF, and six additional races. Resistance was observed to all the races evaluated. Molecular markers confirmed the presence of Sr7a, Sr24, Sr31, Sr38 and Sr57 in bread wheat, and Sr13 in durum wheat. The re-emergence of wheat stem rust in Spain and the occurrence of unique virulences underscore the need to continue surveying and monitoring this disease.  相似文献   

Endogenous regulation of macrophage proliferative expansion by colony-stimulating factor-induced interferon   总被引：19，自引：0，他引：19  

R N Moore  H S Larsen  D W Horohov  B T Rouse 《Science (New York, N.Y.)》1984,223(4632):178-181

Stimulation of cultures of murine bone-marrow cells with specific macrophage growth factor (colony-stimulating factor I) resulted in the production of type I interferon. Neutralization of this endogenous interferon by antiserum directed against interferons alpha and beta resulted in a significant enhancement of mononuclear phagocyte proliferation from committed marrow precursors. The effect of the antiserum was lost in cultures depleted of adherent cells, an indication that an adherent regulatory cell (or cells) in the marrow limits mononuclear phagocyte proliferation by producing antiproliferative interferon in response to high levels of specific growth factor.  相似文献   

Osedax: bone-eating marine worms with dwarf males     

Rouse GW  Goffredi SK  Vrijenhoek RC 《Science (New York, N.Y.)》2004,305(5684):668-671

We describe a new genus, Osedax, and two new species of annelids with females that consume the bones of dead whales via ramifying roots. Molecular and morphological evidence revealed that Osedax belongs to the Siboglinidae, which includes pogonophoran and vestimentiferan worms from deep-sea vents, seeps, and anoxic basins. Osedax has skewed sex ratios with numerous dwarf (paedomorphic) males that live in the tubes of females. DNA sequences reveal that the two Osedax species diverged about 42 million years ago and currently maintain large populations ranging from 10(5) to 10(6) adult females.  相似文献   

Salt Discharge from an Inland Farm for Marine Shrimp in Alabama     

Christopher A.  Boyd Claude E.  Boyd Aaron A.  McNevin D. B. Rouse 《Journal of the World Aquaculture Society》2006,37(4):345-355

The total salt input in saline well water, mineral amendments, feed, and rainfall and runoff to ponds of an inland shrimp farm in Alabama was 1980.8 tonnes over a 5‐yr period. A residual of 270.4 tonnes of salt remained in pond water and 38.3 tonnes in bottom soil. Only 8.0 tonnes of salt were removed in harvested shrimp. A total of 1588.0 tonnes of salt or 80.2% of the input was lost to the environment with about equal amounts exiting the ponds in seepage and in overflow and harvest effluent. About 4.2% of the salt input (84.1 tonnes) could not be accounted for because of errors in assumptions and measurements. Salt concentration was elevated in a small stream passing through the farm and in the shallow aquifer beneath it. Needham Creek, the receiving water body for runoff and base flow from the farm watershed, had elevated salt concentrations when ponds were partially drained for harvest in the fall. At this time, chloride concentration exceeded 230 mg/L, the maximum concentration allowed by Alabama Department of Environmental Management regulations. Greater water reuse or more gradual release of pond effluent during harvest would reduce the peak in‐stream chloride concentration and avoid noncompliance with the in‐stream chloride criterion.  相似文献   

Growout of Pacific White Shrimp, Litopenaeus vannamei, Stocked into Production Ponds at Three Different Ages   总被引：2，自引：0，他引：2  

Oscar  Zelaya  David B.  Rouse  D. Allen  Davis 《Journal of the World Aquaculture Society》2007,38(1):92-101

This study was designed to determine the production characteristics of the Pacific white shrimp, Litopenaeus vannamei, stocked into grow‐out ponds at three different sizes and ages. To meet this goal, three groups of postlarvae (PL) were obtained. The first group was placed in a nursery system for 21 d (N21), the second for 14 d (N14), and the third was stocked directly into ponds (DS). Shrimp from each nursery treatment (three tanks per treatment) were pooled and then subdivided for stocking into four replicate 0.1 ha ponds per treatment, another four ponds were stocked directly (DS) with PL₈. All 12 ponds were stocked on the same day at a density of approximately 35 PL/m², and cultured over a 16‐wk period and then drain harvested. After harvest, mean average weights (15.4, 16.9, and 14.9 g), survivals (63, 62, and 64%), FCRs (2.7, 2.5, and 2.7), and average yields (3592, 4005, and 3374 kg/ha) were determined for N21, N14, and DS, respectively. No significant (P > 0.05) differences were observed among treatments. Regardless of nursing time, nursed juveniles did not differ significantly in production characteristics from shrimp stocked directly from the hatchery.  相似文献   

Influence of Nursery Period on the Growth and Survival of Litopenaeus vannamei Under Pond Production Conditions   总被引：2，自引：0，他引：2  

Antonio  Garzade Yta  David B.  Rouse D. Allen  Davis 《Journal of the World Aquaculture Society》2004,35(3):357-365

Techniques for head starting or nursing postlarvae (PL) has received considerable attention with regards to nursery protocols, yet there is little data pertaining to the effects of nursery period on the final growout of shrimp to marketable size. This study was performed to investigate the influence of nursery duration on survival and growth of Litopenaeus vannamei during subsequent pond culture. For this research, a single population of high health PL were received from a commercial hatchery and held in a tank for acclimation, quantification, and distribution to nursery tanks or ponds. Treatments included direct stocking of 10-d-old postlarvae (PL₁₀) into production ponds as well as the nursing of PL in a covered greenhouse nursery system for an additional 10 or 20 d. After nursing, the PL were harvested, quantified, and transferred to growout ponds. All ponds were stocked at a density of 35 PL/m² and maintained under standardized conditions. Shrimp were fed with a 35% protein shrimp feed, twice daily during the 112-d growth trial. Ponds were aerated as needed using a maximum of 19 hp/ha to maintain adequate dissolved oxygen (DO > 3.0). No statistical differences (P >0.05) were found in survival, yield, or growth between treatments. At harvest, survivals during growout were generally higher in ponds with nursed shrimp (77% for PL₂₀ and 79% for PL₃₀) than in ponds receiving PL₁₀ shrimp (67%). Yields were similar between treatments, ranging from 3,525 for direct stocked shrimp to 3,747 kg/ha for those that were nursed for 10 d. Although growth rates of PL under pond conditions will be faster than that of a nursery system, results suggest that a nursery period of at least 10 d helps improve survival during pond production and promotes better size uniformity. Shrimp nursed for 20 d showed little improvement in survival over shrimp nursed for 10 d but did result in a more uniform size of shrimp at harvest.  相似文献   

Effects of Potassium, Magnesium and Age on Growth and Survival of Litopenaeus vannamei Post-Larvae Reared in Inland Low Salinity Well Waters in West Alabama   总被引：2，自引：0，他引：2  

D. Allen  Davis  Claude E.  Boyd David B.  Rouse I. Patrick  Saoud 《Journal of the World Aquaculture Society》2005,36(3):416-419

Abstract— The production of Litopenaeus vannamei in inland low‐salinity well water is a growing industry in several regions of the world. The state of Alabama in the southeastern USA is one such region with a large saline aquifer that could be utilized for shrimp culture. However, some farmers are experiencing problems rearing marine shrimp while others are having considerable success. Previous work has correlated low levels of potassium andor magnesium to poor shrimp survival. The problem is further complicated by the fact that the age at acclimation may also influence survival. In our present study, we evaluated the effects of potassium, magnesium, and the age of acclimation on growth and survival of PL at two farms. The first experiment was run in a static system utilizing four replicate tanks per treatment. Fifty PL₁₇ (0.0066 g) that had been acclimated to 4 ppt seawater were stocked into each tank and the following treatments evaluated: low salinity well water (LSWW) without mineral supplements, LSWW with KCl, LSWW with MgCl₂, and LSWW with KCl and MgCl, added to the water. Shrimp were harvested, counted and weighed after 4 wk. Survival was significantly higher in treatments receiving mineral supplements whereas biomass was only higher in the two treatments with potassium supplements. The second experiment was set up initially as a static system filled with 8.5‐ppt reconstituted sea water that was then converted to a flow‐through system using LSWW. This experiment evaluated the effect of PL age at acclimation on survival and growth at four different ages (PL₁₅, PL₁₉, PL₂₃, and PL₂₇). All tanks were stocked with 50 PL₁₃L. vannarnei. Two days after stocking, and then at 4‐d intervals, a series of four tanks were converted to flow through (rate of 40 Lhr) using LSWW. After acclimation, water flow was maintained in all tanks until 28 d after stocking when tanks were harvested and surviving shrimp were counted and weighed. Survival and growth increased with PL age when shrimp were acclimated to inland low salinity well water.  相似文献