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11.
对采集自我国12个不同分布区的105个野生披碱草属(Elymus)牧草居群的14项穗部形态指标进行研究,结果表明,我国披碱草属牧草种质资源穗部遗传多样性丰富,居群内多样性指数(H′)在0.784~1.652,平均多样性指数为1.396;居群总平均多样性指数为1.936,居群间表型分化系数为27.89%。14项穗部形态性状在居群内多样性、居群总多样性以及变异系数变化不完全一致,居群内H′最高的为旗叶与穗基部长度(1.648),居群总H′最高的为小穗长(2.054),变异系数最大的为第一颖芒长(58.932%),平均变异系数为31.22%,表明穗部形态性状均存在较大的变异,F检验差异极显著。引起居群形态差异的性状与种子产量密切相关,主要包括穗长、穗宽、第一颖芒长、第一外稃长、第一内稃长、第一外稃芒长、每穗轴小花数、第一颖长和第一颖宽。我国野生披碱草属牧草的多样性和分布格局是相关的,我国西北部是野生披碱草属牧草分布中心,也是披碱草属牧草的多样性中心。此外,提出了我国披碱草属牧草保护的策略。 相似文献
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本试验旨在研究噬菌体复合制剂对肉鸡生长性能、免疫功能、肠道形态及肠道菌群的影响。选取14 000只体重(850±45) g的37日龄青脚麻鸡,随机分为2组,分别是对照组(基础饲粮)和试验组(基础饲粮+109PFU/mL的噬菌体复合制剂),每组7个重复,每个重复1 000只,试验期27 d。结果表明:1)与对照组相比,试验组的料重比下降2.81%(P>0.05)。2)与对照组相比,43日龄时,试验组肉鸡胸腺指数和脾脏指数分别增加16.80%(P<0.05)和7.14%(P>0.05);52日龄时,肉鸡法氏囊指数和胸腺指数分别增加8.55%(P>0.05)和7.37%(P>0.05);61日龄时,肉鸡法氏囊指数增加39.66%(P>0.05);43、52及61日龄时,试验组肉鸡血清免疫球蛋白G(IgG)含量分别增加15.85%(P>0.05)、29.54%(P>0.05)和1.45%(P>0.05)。3)与对照组相比,43、52及61日龄时,试验组肉鸡十二指肠、空肠、回肠绒毛高度/隐窝深度显著提高(P<0.05)。4)在门水平上,... 相似文献
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Linnajara DE VASCONCELOS MARTINS FERREIRA Fernanda DE CARVALHO Jlia FONSECA COLOMBO ANDRADE Damiany PDUA OLIVEIRA Flvio Henrique VASCONCELOS DE MEDEIROS Fatima Maria DE SOUZA MOREIRA 《土壤圈》2020,30(1):98-108
Production of common bean(Phaseolus vulgaris)is limited by the occurrence of damping off(rhizoctoniosis),which is caused by the fungus Rhizoctonia solani.However,the co-inoculation of plant growth-promoting rhizobacteria(PGPR)involved in biological control along with diatomic nitrogen(N2)-fixing rhizobia can enhance N nutrition and increase production.In this context,finding microorganisms with synergistic effects that perform these two roles is of fundamental importance to ensure adequate yield levels.The aim of this study was to evaluate the effects of co-inoculation of nodule endophytic strains of the genera Bacillus,Paenibacillus,Burkholderia,and Pseudomonas with Rhizobium tropici CIAT 899,an N2-fixing rhizobial strain,on the biocontrol of damping off and growth promotion in common bean plants.Greenhouse experiments were conducted under axenic conditions using the common bean cultivar Pérola.The first experiment evaluated the potential of the 14 rhizobacterial strains,which were inoculated alone or in combination with CIAT 899,for the control of R.solani.The second experiment evaluated the ability of these 14 rhizobacterial strains to promote plant growth with three manners of N supply:co-inoculation with CIAT 899 at low mineral N supply(5.25 mg N mL^-1),low mineral N supply(5.25 mg N mL^-1),and high mineral N supply(52.5 mg N mL^-1).The use of rhizobacteria combined with rhizobia contributed in a synergistic manner to the promotion of growth and the control of damping off in the common bean.Co-inoculation of the strains UFLA 02-281/03-18(Pseudomonas sp.),UFLA 02-286(Bacillus sp.),and UFLA 04-227(Burkholderia fungorum)together with CIAT 899 effectively controlled damping off.For the common bean,mineral N supply can be replaced by the co-inoculation of CIAT 899 with plant growth-promoting strains UFLA 02-281/02-286/02-290/02-293.Nodule endophytes UFLA02-281/02-286 are promising for co-inoculation with CIAT 899 in the common bean,promoting synergy with rhizobial inoculation and protection against disease. 相似文献
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Abstract. Viral haemorrhagic septicaemia (VHS) is reported for the first time in sea-water cultured rainbow trout. Heavy mortalities with typical signs and lesions A VHS virus (serotype 1) was isolated from the diseased fish. The mortalities were caused only by the VHS virus and 80 days post transfer of trout to sea-water the mortalities reached 85%, of the initial population.
The disease was experimentally transmitted to rainbow trout, both in sea-water 3·104 pfu/ml of virus or by intramuscular injection of various doses of VHS1 (7·101 7·104 or 7·104 pfu per fish). Death occurred in all infected groups and started earlier in sea-water. Typical signs of VHS were observed in moribund fish. Viral multiplication was demonstrated to have occurred in fish organs. 相似文献
The disease was experimentally transmitted to rainbow trout, both in sea-water 3·10
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The effectiveness of the Californian model Bugoff2 for Cydia pomonella was tested in Germany (4 years) and Italy (2 years), the Italian results confirming the German findings. In the first codling moth generation (air temperatures normally below 30 C) good agreement was found between field observations of egg hatching and the model's forecast. However, the model gave notable differences for the second generation, i.e. 3 weeks in Germany and 1 week in Italy, and a significant difference for the third generation in Italy. These divergencies arise from an overestimation of the development rate of the codling moth in Bugoff 2's heat unit table whenever air temperature exceeds the upper limit for development. As a result of modifications to Bugoff 2, an example of a better simulation of the codling moth egg hatch in Germany and Italy is discussed. 相似文献
19.
The standard ELISA method for detection of PVY in dormant potato tubers was compared with recently developed methods: (1) a hybridization technique, using a complementary DNA-probe to PVYN; (2) three modifications of ELISA (standard monoclonal, Flegg & Clark polyclonal or monoclonal). Sap of six Dutch cultivars secondarily infected with PVY was subjected to these methods directly after lifting and after six subsequent one-week intervals during storage at 20°C. During storage the detectability of PVY decreased, irrespective of the diagnostic method applied. However, decrease was less in susceptible than in resistant cultivars. Although the hybridization technique yielded slightly better results than the standard ELISA procedure, it was time-consuming and involved hazardous reagents. The modifications of ELISA investigated were not, in general, more sensitive than standard ELISA. 相似文献
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