樱桃透光和郁闭树冠相对光照强度及其果实品质和产量的差异   总被引：6，自引：2，他引：4  

王小伟  尚志华  张强  魏钦平  刘军 《园艺学报》2009,36(2):157-162

 以13年生‘红灯’甜樱桃（Prunus avium L.）为试材,应用树冠分格方法,研究了树冠透光和郁闭两种冠型内相对光照强度及其果实品质和产量的差异,以及相对光照强度与果实产量品质的关系。结果表明,两种树冠内相对光照强度均自下而上逐渐增高,透光和郁闭树冠小于30%的相对光照区域分别占树冠总体积的25.23%和52.78%。透光树冠果实分布均匀,主要集中在树冠1.5～2.5 m的高度;而郁闭树冠果实分布差异较大,主要在树冠的外围和上部;产量分别是9.02t.hm-2和3.53t.hm-2。果实品质因素与相对光照强度的回归分析表明樱桃单果质量、果实硬度、果实可溶性固形物含量、可滴定酸含量、固酸比最佳的相对光照强度值分别为76.52%、46.84%、100.00%、41.63%和75.77%。  相似文献   

阔叶猕猴桃果实GalDH cDNA 克隆及其在大肠杆菌中的表达   总被引：2，自引：0，他引：2  

尚增振  王小华  马锋旺  梁东 《园艺学报》2009,36(12):1741-1748

 以猕猴桃属植物中果实维生素C含量最高的阔叶猕猴桃(Actinidia latifolia Merr. ) 果实为试 材, 经RT2PCR扩增获得约110 kb的L - 半乳糖脱氢酶cDNA片段。生物信息学分析表明, 该cDNA片段长为997 bp, 最大开放阅读框为960 bp, 可编码319 个氨基酸残基, 命名为A lGalDH, GenBank登录号为EU525846, 核苷酸序列及其推导的氨基酸序列与已知其它植物GalDH核苷酸、氨基酸序列间的同源性分别在76%和70%以上, 且具有醛酮还原酶的保守结构域。构建了其原核表达载体pET-A lGalDH并转化大肠杆菌BL21, 经0.1 mmol·L ^{- 1} IPTG诱导, 获得具有较高活性的表达融合蛋白6 ×His-AlGalDH。经Ni-His亲和磁珠分离纯化, 获得单一的融合目的蛋白条带, 测定其酶活为120 pmol·min^{- 1} ·mg^{- 1}。  相似文献   

Effect of atorvastatin on neointimal hyperplasia of venous autografts in rats     

SONG Zi-fang  LI Wei  ZHENG Qi-chang  SHANG Dan  XIONG Jun  GUAN Si-ming 《园艺学报》2009,25(4):631-635

AIM: To investigate the effect of atorvastatin on neointimal hyperplasia of autogenous vein graft in rats. METHODS: The model of autogenous vein graft was prepared by transplanting the external jugular vein into aorta in Wistar rats. The rats were divided into three groups: sham operation group, graft control group and graft experimental group. From three days after transplantation, the rats of autograft experimental group were treated by atorvastatin at a dosage of 5 mg·kg-1·d-1. Four weeks after treatment, venous autografts were removed at autopsy and cut into 4 μm sections. Histopathological examination was carried out to analysis the neointimal hyperplasia of grafted veins. Immunohistochemical staining was conducted to evaluate SMα-actin and PCNA expression of neointimal cells in venous autografts. RESULTS: In venous autograft control and experimental groups, SMα-actin-positive smooth muscle cells were proliferated and accumulated excessively in venous autografts, which resulted in significant neointimal formation and vascular lumen narrowing. Neointima quantitative assay revealed that the neointimal hyperplasia of venous autografts was suppressed obviously in graft experimental group, and its neointimal area and NIA/MA ratio of venous autografts were significantly lower than those in graft control group (P<0.01). Immunohistochemical assay indicated that the PCNA labeling index of neointimal cells was significantly lower in graft experimental group than that in graft control group (P<0.01). CONCLUSION: Atorvastatin significantly inhibits the proliferation of neointimal smooth muscle cells and the development of neointimal hyperplasia of venous autografts in rats. Atorvastatin is a powerful inhibitor of restenosis after vascular reconstructive operation with a potential for therapeutic use.  相似文献   

板叶红瓤萝卜新品种‘潍萝卜2号’   总被引：2，自引：1，他引：1  

韩太利  杨晓东  徐立功  谭金霞  宋银行 《园艺学报》2009,36(1):151-151

 ‘潍萝卜2号’萝卜新品种是由‘VL02-21’בVL02-23’杂交育成的萝卜一代杂种。该品种优质、丰产、适应性强。从播种到商品成熟80d,生长势中等,抗病毒病、霜霉病和软腐病等病害,板叶,叶色深绿有12-16片,小顶小根,肉质根近圆球形,肉质鲜艳呈紫红色,单根重1㎏,产量36.72～41.68 t·hm^-2,适合山东及华北地区和东北三省秋季种植。  相似文献   

In vitro differentiation of exocrine pancreatic cells from mouse embryonic stem cells     

REN Meng  MIN Jun  YAN Li  SHANG Chang-zhen  CAO Jun  LI Jing-yi  CHENG Hua 《园艺学报》2009,25(8):1585-1590

AIM: To explore the effects of sodium butyrate, activin A and dexamthasone on inducing mouse embryonic stem (ES) cells to differentiate into exocrine pancreatic cells in vitro. METHODS: E14 mouse ES cells were cultured in suspension to form embryonic bodies (EBs). The EBs were cultured with differentiating medium containing different concentrations of sodium butyrate, and the spontaneously differentiated ES cells were used as control. Exocrine pancreatic genes such as amylase, chymotrypsinogen, elastase 1, elastase 2 and carboxypeptidase were detected by RT-PCR at different time points to determine the optimal concentration and exposure time of sodium butyrate. Furthermore, activin A or dexamthasone was also used to explore the effects on exocrine differentiation. After that, the combination of sodium butyrate, activin A and dexamthasone was used to promote the differentiation of exocrine pancreatic cells from ES cells. During the differentiation course, the gene expressions of amylase, chymotrypsinogen, elastase 1, elastase 2 and carboxypeptidase were detected by RT-PCR. Morphological changes were investigated by phase contrast microscopy. Amylase expression was examined by immunofluorescence staining. RESULTS: Exocrine pancreatic gene expressions such as amylase, chymotrypsinogen, elastase 1, elastase 2 and carboxypeptidase were detected in spontaneously differentiated EBs. A relatively lower concentration of sodium butyrate with a shorter exposure time significantly promoted those above gene expressions as compared to that of spontaneously differentiated EBs. Activin A and dexamethasone induced upregulation of exocrine gene expression. The combination of activin A, sodium butyrate and dexamethasone significantly enhanced the mRNA levels of amylase, chymotrypsinogen, elastase 1, elastase 2 and carboxypeptidase. Under the treatment of activin A, sodium butyrate and dexamethasone, differentiated cells were polygonal in shape with large, round, and center-situated nuclei. According to the observation of immunofluorescence staining, amylase was positive expressed at the final stage. CONCLUSION: These data indicate that exocrine pancreatic differentiation of ES cells is induced by sodium butyrate, activin A and dexamethasone. The combination of pancreatic inducing factors improves the differentiating efficiency.  相似文献   

黄连、黄芩提取液与二氧化氯复配对结球甘蓝种传黑斑病的抑菌效果研究     

易旸  孙成虎  尚庆茂  张志刚 《北方园艺》2009,(5)

采用三元二次回归正交旋转组合设计方法,确立了黄连、黄芩提取液与二氧化氯对甘蓝链格孢菌抑制效果最佳的复配组合:黄连提取液13.48%、黄芩提取液15.56%、二氧化氯0.14%.用此复配剂浸种,芸苔链格孢菌和甘蓝链格孢菌的侵染率分别降低了97.33%和94.85%,且提高了种子发芽势及萌发相关酶活性.  相似文献   

早熟金针菇新品种G1的杂交选育   总被引：1，自引：0，他引：1  

徐珍  尚晓冬  郭倩  王瑞娟  李玉  谭琦 《食用菌学报》2009,16(4)

以金针菇菌株F3-31和FM-83为亲本,经过杂交选育出优良白色金针菇菌株G1.以菌丝生长速度、生长势、产量、子实体农艺性状及栽培周期等为指标,对金针菇菌株G1与其亲本进行比较.试验结果表明,G1菌丝生长速度快且生长势旺盛;平均单瓶产量达216 g,比亲本分别增产54 g、20 g,生物转化率也比亲本菌株高,达到72%;栽培周期43 d,比亲本分别缩短1 d、6 d;子实体各项农艺性状优良,是适于金针菇工厂化栽培的早熟白色优良菌株.  相似文献   

山东省沙地葡萄茎痘相关病毒的检测   总被引：1，自引：0，他引：1  

尚佑芬  王升吉  赵玖华  张家魁  吕志华  路兴波  孙红炜  杨崇良 《果树学报》2009,26(2)

为明确沙地葡萄茎痘相关病毒(GRSPaV)在山东省发生的情况,2006-2007年在葡萄主栽区和葡萄品种资源圃进行了田间调查、样品采集和病原检测。应用GRSPaV外壳蛋白基因表达获得的多克隆抗体,经间接酶联免疫吸附试验(PTA-ELISA)和斑点酶联免疫吸附试验(Dot-ELISA)检测,在2a采集的298个样品中,125个样品为阳性反应,阳性率41.95%。125个阳性样品涉及43个品种,品种被侵染率74.14%。研究对检测的样品用RT-PCR法验证,有3对引物均扩增出了预期片段,分别为解旋酶基因340bp片段、外壳蛋白(CP)基因842bp片段和RNA聚合酶(RdRp)基因499bp片段。  相似文献   

东方红-YZDZ80C液压自行式移动电站     

尚彩红  邢清奇 《拖拉机与农用运输车》2009,36(3)

介绍东方红-YZDZ80C液压自行式移动电站的主要技术参数和主要部件结构性能特点。  相似文献   

论服饰广告的审美创作     

尚端武 《湖南农机》2009,36(9)

服饰广告的本体功能是促进服饰商品的销售。设计师借助美学手段进行审美创作,能巧妙地融服饰广告的经济价值于审美价值之中,从而达到吸引受众眼球,提升服饰商品的知名度、美誉度,实现销售商品的终极目的。从美学的角度对服饰广告创意的表现手法进行审视,有助于指导具体的广告创作实践,提升服饰广告的审美价值,实现美学与服饰广告的完美结合。  相似文献