花叶良姜组培无菌体系的建立     

刘玉军  刘芳  孙灿岳  俞建妹  沈遐 《北方园艺》2017,(5):89-92

以花叶良姜袋苗为试材,根茎腋芽为外植体,研究外植体预处理、灭菌消毒和外植体长度等因素对其无菌体系建立的影响。结果表明:实验室水培植株3d,根茎腋芽长3~5cm作外植体,用1.25‰HgCl_2灭菌消毒16min,花叶良姜外植体污染率24.2%,萌芽率72.7%,较有利于建立花叶良姜无菌体系。  相似文献   

新生牛血清促细胞生长活性检测方法的建立与应用     

庄金秋  梅建国  管宇  苗立中  沈志强 《中国奶牛》2011,(6):31-37

牛血清是医药生物技术产品中重要的原辅材料之一,做好牛血清的质量控制是提高生物制品质量的重要环节。本研究通过应用PK15、BHK21和ST三种兽用生物制品研制中常用的细胞作为载体,选用国内同等价位5个不同厂家生产的新生牛血清作为比较对象,采用细胞倍增时间测定法、细胞克隆形成率测定法、细胞三次连续传代培养法、MTT比色法和微量终点稀释法五种血清质量鉴定的方法进行比较,检测新生牛血清的促细胞生长活性,最终确定了MTT比色法作为便捷高效快速筛选新生牛血清的检测方法。  相似文献   

双黄连制剂的药理作用及在兽医临床中的应用   总被引：1，自引：0，他引：1  

张志美  沈志强  郭时金  张颖  刁鹏飞  王艳萍 《动物医学进展》2010,31(4):95-98

双黄连制剂为金银花、黄芩、连翘三味中药精制而成的纯中药制剂,具有辛凉解表、清热解毒之功效。根据现代药理研究和动物试验证实,双黄连制剂的药理作用主要有抗菌、抗病毒、解热抗炎及增强机体免疫力等。兽医临床上对感冒发热、细菌、病毒及气候变化引起的家禽、家畜及宠物等的各种呼吸道疾病及细菌感染引起的胃肠道疾病等均有明显疗效。随着研究的不断深入,双黄连制剂在兽医临床上将会得到广泛的应用。目前,兽医临床上现有剂型主要为口服液、可溶性粉及注射液等。论文对双黄连制剂的药理、毒理及在兽医临床上的应用进行了综述,为双黄连制剂的药理作用的研究及临床应用提供依据。  相似文献   

Effect of 3,5-hydroxy-6,7,3’,4’-tetramethoxyflavone isolated from Laggera pterodonta on apoptosis of Hep-2 cells in vitro     

CAO Chang-shu  SHEN Wei-zhai  LI Yao-lan  WANG Hui 《园艺学报》2010,26(7):1362-1365

AIM: To investigate the effect of 3,5-hydroxy-6,7,3’,4’- tetramethoxyflavone (HTMF) isolated from Laggera pterodonta on Hep-2 cell apoptosis and the underlying mechanism. METHODS: The MTT assay was used to observe the cytotoxicity of HTMF to the normal cells and the inhibitory effect of HTMF on the proliferation of tumor cells. The apoptosis was determined by flow cytometry. Western blotting was used to detect the expression of caspase-3 and caspase-9. RESULTS: HTMF significantly inhibited the growth of Hep-2 cells in dose and time dependent manners. HTMF exhibited weak cytotoxicity to the two normal cell lines Vero and EVC304, while showed low effect of anti-proliferation on HepG2 cells and A549 cells. The increase in apoptosis of Hep-2 cells by HTMF was observed with dose and time dependent manners. Western blotting showed that HTMF time dependently increased the expression of caspase-3 and caspase-9 in Hep-2 cells. CONCLUSION: HTMF has high inhibitory effect on the proliferation of Hep-2 cells by induction of apoptosis in the tumor cells through caspase-9 and caspase-3 activation. However, the cytotoxicity of HTMF to the normal cells is low.  相似文献   

茭白配方专用肥肥效比较试验   总被引：1，自引：0，他引：1  

朱徐燕  沈建国  庞英华  王忠  朱建杰  黄锡志 《中国园艺文摘》2013,(5):18-20

通过与常规施肥比较,研究茭白配方专用肥对茭白生物学性状、产量和品质的影响。试验结果表明:茭白商品性有明显改善,粗脂肪、维生素C、总糖、氨基酸等品质指标增幅达到极显著水平,并可实现增产增效21.96%,节约肥料成本6.53%,利润提高25.98%。在配方专用肥的基础上增施氮肥,不能提高茭白产量,且肥料成本增加,种植效益明显下降。  相似文献   

一种改进的基因表达数据分类方法     

蔡立军  沈小乔  林亚平  蒋林波 《湖南农业大学学报(自然科学版)》2007,34(3)

从分类算法和特征基因选择两个方面研究基因表达数据的分类,将传统的Support Vector Machines(SVM)算法和K-nearest neighbor(KNN)算法两者结合成为一种应用于基因表达数据分类的算法,并针对基因表达数据分类数据集“样本少,维数高”的特点,提出了一种改进的基于相关性的递归特征消除算法(简称为C-RFE),消除了数据冗余．实验结果表明,新方法可有效提高分类准确率和特征选取的效率．  相似文献   

Effects of radix pseudostellariae on cardiac function and matrix metalloproteinases in rat chronic heart failure induced by coronary artery ligation     

SHEN Xiang-chun  TAO Ling  PENG Jiao  FANG Tai-hui 《园艺学报》2008,24(9):1694-1698

AIM:To investigate the effects of radix pseudostellariae on cardiac function and matrix metalloproteinases (MMPs) in rat chronic heart failure induced by coronary artery ligation. METHODS:Rat model of chronic heart failure was produced by ligating the coronary artery for 6 weeks. The cardiac functions were determined by observing the hemodynamics parameters. mRNA expressions of MMP-2 and MMP-9 in left ventricular tissue were detected by RT-PCR and activities of MMPs were assayed by zemography.RESULTS:Cardiac function was remarkable turbulence after coronary artery ligation 6 weeks, according to the results of the hemodynamics. The mRNA contents and activities of MMP-2 and MMP-9 in left ventricular tissue were significantly elevated. Radix pseudostellariae remarkable ameliorated the cardiac functions, inhibited the mRNA contents and activities of MMP-2 and MMP-9 in left ventricular tissue of rat with chronic heart failure after continued 5 weeks treated with decoction of radix pseudostellariae. CONCLUSION:The decoction of radix pseudostellariae ameliorates cardiac functions of rat with chronic heart failure. The mechanism maybe involves in the inhibition of the MMP-2 and MMP-9 mRNA expression and activities in cardiac ventricles.  相似文献   

The expressive levels of CD14⁺ CD16⁺ monocytes change in kwasaki disease     

SUN Fei  GU Zai-yan  DING Ren-ye  SHEN Wen-juan 《园艺学报》2008,24(10):2042-2043

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盐度与滤料量对生物膜降氨氮作用的影响          下载免费PDF全文

朱正国  臧维玲  戴习林  彭自然  罗春芳  沈俊  马蓉  丁福江 《上海海洋大学学报》2007,(6):537-541

淡水中培养的生物膜,去氨氮能力随盐度上升及升盐速率的增加而逐渐下降,当盐度由0.6升至13.3、23.9和31.3并稳定48 h后,氨氮去除率分别为100%、93.0%和86.9%;当按不同的速度降盐至淡水环境时,生物膜去氨氮的能力逐步得以恢复,特别是降到淡水时,去氨氮率均达90%以上,基本达到淡水中生物膜去氨氮的能力。经24~36 h的吸附作用,沸石对氨氮的吸附能力强于瓷质生化环,生化环48 h去氨氮的能力(95.1%)接近沸石(100%)。以沸石为滤料的滤器去氨氮能力与速率随沸石用量增加而增强。24 h内天然沸石吸附作用去氨氮能力强于生物沸石,生物沸石48 h对氨氮的去除率(99.2%)略超过天然沸石(95.2%)。  相似文献   

外出打工对中部农村留守家庭社会适应的影响分析——农村社区关系资源网络代际继替裂痕对家庭的影响     

周献德  沈新坤 《河北农业科学》2009,13(11):138-140,163

在缺乏外部社会保障有效介入的农村,依靠地缘和血缘关系建立的社区关系资源网络为农村家庭提供着有效的社区保障。随着农村大规模、长期性的外出进城打工,引发家庭主导群体的家庭撤离和社区撤离,使得具有独特个人性和代际继替特征的社区关系资源网出现代际继替裂痕,这对农村家庭的社区支持将产生重要影响。从社区关系资源网代际继替的角度浅析外出打工对农村家庭生活的影响。  相似文献