In vitro growth inhibition effects of rhHGF/cHGF on SMMC-7721 human HCC cell line     

YU Wei-ping  WEI Kai-bin  GAO Lei  SHEN Cheng-xing  ZHANG Rong  WANG Guo-zhen 《园艺学报》2002,18(3):256-258

AIM:To examine the effects of recombinant human hepatocyte growth factor(rhHGF) and native calf HGF(cHGF) on SMMC-7721 human hepatocellular carcinoma(HCC) cell line. METHODS:Human HCC cell line culture, photometric assay, and flow cytometric assay were used in this study .RESULTS:A similar type of dose-dependent cell growth inhibition effect on SMMC-7721 human HCC cells by rhHGF(5-20 μg/L) as well as by cHGF(25-100 mg/L) had been found, with the maximal effect at the highest concentration used. Approximately over 50% of the cells treated with rhHGF(5 μg/L, 10 μg/L, 20 μg/L) accumulated in the quiescent G₀/G₁ phase of the cell cycle over incubation periods for 3 d. CONCLUSION:The growth of SMMC-7721 human HCC cells was strongly inhibited by both rhHGF and cHGF. This might be because the cells exposed to HGF became arrested in the G₀/G₁ phase.  相似文献   

Effects of Taoren Honghua Jian on the hyperlipidemia without symptom     

CHEN Xiao-yin  ZHANG Qun  YANG Qin-he  XIE Qiu-ling  SHEN Qiang  XU Yun-sheng 《园艺学报》2002,18(12):1529-1531

AIM: To examine the effect of traditional chinese medicine recipe, Taoren Honghua(semen persicae-flos carthami) decoction, on hyperlipidemia without symptom. METHODS: The plasma TC, TG, LDL, HDL, apolipoprotein(Apo) A, Apo Bof the patients with hyperlipidemia without symptom were measured using automatic analyzer (shimadiu CL-7200), the production of nitric oxide(NO) was detected by Greiss reaction, and SODactivity and MDAformation were examined using o-trihydroxy benzene and barbituric methotheds, respectively. RESULTS: After oral administration of Taoren Honghua decoction, the plasma levels of TC, TG, LDL, and MDAof the patients were markedly decreased, however, the plasma levels of ApoA, HDL, SODand NOwere significantly increased and almost no change was detected in the plasma level of Apo B. In control group, it was found that although the plasma level of TC, TG and LDL were decreased ( P<0.05 ) and ApoAas well as HDLwere increased, ApoB, SOD, MDA and NO production were all unchanged. CONCLUSION: The traditional chinese medicine recipe, Taoren Honghua decoction,has a significant therapeutic effect on patients with hyperlipidemia by removing blood stasis, promoting qi circulation and in turn reducing blood lipid level.  相似文献   

施氮量对棉田主要害虫种群动态的影响   总被引：5，自引：0，他引：5  

崔金杰  夏敬源  王春义  马丽华  雒君瑜 《中国植保导刊》2003,23(1):3-5

通过不同施氮肥量对棉田主要害虫种群动态影响的研究结果表明,在低、中、高3种施氮肥水平处理条件下,随着氮肥量的增加,棉田棉铃虫落卵量增加,棉苗蚜、棉叶螨种群数量减少,发生为害减轻,但棉伏蚜种群数量增加。中等施氮肥条件下,第二、三代棉铃虫幼虫数量高于低和高施氮肥处理,对棉铃虫幼虫的生长发育和存活较为有利。  相似文献   

棉疫病菌elicitin基因的克隆与序列分析   总被引：1，自引：0，他引：1  

申贵  王源超  郑小波 《植物病理学报》2003,33(6):559-562

 棉疫病菌是我国棉花和苎麻疫病的主要病原菌,引起棉花烂铃、死苗,苎麻叶斑和茎腐,严重影响棉花和苎麻的产量和品质^[1,2],该菌的主要寄主有棉花、苎麻和构树^[3]。  相似文献   

禽多杀性巴氏杆菌强毒株与弱毒株制备的蜂胶灭活疫苗免疫原性的比较试验     

沈志强  刘吉山  徐可利  李峰  吴信明  林初文  赵蕾 《中国兽药杂志》2004,38(5):14-16

用禽多杀性巴氏杆菌强毒株(C48-1)与弱毒株(G190E40和B26-T1200)制备的蜂胶灭活疫苗的免疫原性对比试验结果表明,强毒株C48-1制备的禽霍乱蜂胶灭活疫苗的免疫效果显著高于弱毒株G190E40和B26-T1200,近期保护率相差20%～40%,3个月后相差40%～80%.强毒株C48-1的免疫原性显著高于弱毒株G190E40和B26-T1200,说明禽多杀性巴氏杆菌荚膜上的毒力蛋白可能具有免疫原性.  相似文献   

应用小白鼠代替鸡对禽霍乱蜂胶灭活疫苗进行效检的研究     

刘吉山  沈志强  王艳  林初文  李峰  赵蕾  刘万成 《中国兽药杂志》2004,38(6):7-9

为寻找其他有效的禽霍乱蜂胶灭活疫苗的效检方法,应用小白鼠代替本动物鸡对禽霍乱蜂胶灭活疫苗进行效检.结果表明,小白鼠免疫时体重15～18 g,疫苗免疫剂量0.3 mL/只,免疫10 d后攻毒,攻毒时体重20～25 g,攻毒剂量5～9 CFU/只时,可代替本动物鸡对禽霍乱蜂胶灭活疫苗进行效检,判定标准为免疫组8/10以上保护,对照组5/5死亡为合格.本效检方法可缩短检验期限7 d,降低检验成本,具有敏感、特异、客观、简便等特点.  相似文献   

姬松茸水溶性多糖ABP-Ⅱ-a的理化性质     

沈爱英  谷文英 《食用菌学报》2004,11(2):7-11

从姬松茸子实体的热水提取物中分离纯化出组分ABP-Ⅱ-a。高压液相层析测得其相对分子量为52500，红外扫描显示有典型的多糖吸收峰。气相色谱分析表明，其单糖组成为葡萄糖、甘露糖、半乳糖和少量阿拉伯糖，摩尔比为35．24：5．19：1．91：1。  相似文献   

稻曲病侵染时期和流行因素研究   总被引：3，自引：0，他引：3  

沈卫新 《中国植保导刊》2004,24(10):9-11

分期接种试验的结果表明,稻曲病对水稻的侵染时期,主要在孕穗末期至破口期,病菌侵入籽粒在水稻开花前。3a的系统调查和对比分析结果说明,水稻穗期最高气温>32℃的天数少,降雨量大,有利病菌的侵入和发展流行,品种间抗(耐)病性差异显著。近年来稻曲病发生严重的主要原因是单季晚稻的大面积种植,穗期气候条件适宜病害发生流行。  相似文献   

Relation between TNF-α,TNFR I expression and apoptosis in oral lichen planus     

SHEN Li-Jia  RUAN Ping  YIN Cao  XIE Si-ming  ZHAO Tong 《园艺学报》2004,20(9):1664-1667

AIM: To examine the expression and distribution of tumor necrosis factor-α (TNF-α), tumor necrosis factor receptor I (TNFR I) and apoptosis in oral lichen planus, and evaluate their roles and relation in the oral lichen. METHODS: Immunohistochemical technique and TUNEL were employed to study the expression of TNF-α, TNFR I and apoptosis in 50 cases of oral lichen planus and 10 normal oral mucosa specimens. RESULTS: Compared with the normal control group, TNF-α expression was upregulated in mononuclear cells in lamina propria and decreased in keratinocytes in oral lichen planus lesion (P<0.05). On the contrary, TNFR I expression was increased in keratinocytes and decreased in lamina propria in oral lichen planus lesion (P<0.05). The increased apoptosis index in keratinocytes and the decreased apoptosis index in lamina propria were found in oral lichen planus (P<0.05). CONCLUSION: The accelerated apoptosis of keratinocytes and the inhibition of lymphocytes apoptosis may contribute to the formation and progression of oral lichen planus.  相似文献   

Ginkgo biloba extract enhances c-jun expression and attenuates motoneuron death induced by root avulsion     

LI Fang-lan  ZHOU Li-hua  YUAN Qun-fang  YAO Zhi-bin  WU Wu-tian 《园艺学报》2004,20(10):1783-1785

AIM: To investigate the influence of Ginkgo biloba extract (EGb761) on c-jun expressions and motoneurons survival following root avulsion. METHODS: One hundred and eighty adult Sprague-Dawley female rats were randomly divided into control and EGb761 groups. Immediately after avulsion of C5-T1 nerve roots, the rats were injected ip with either 1 mL of EGb761 25 mg·kg^-1·d^-1 or the same volume of normal saline, and the treatment repeated everyday. At 4 h to 6 weeks following avulsion, the C7 spinal segments of all rats were collected and prepared for c-jun immunocytochemistry and neutral red stain. The numbers of c-jun positive and survival motoneurons were counted and compared between two groups at each time point. RESULTS: In control rats following avulsion, c-jun positive motoneurons appeared at 4 h, reached its maximum at 1 d and declined to 2 weeks. Avulsion-induced motoneurons death started at 2 weeks, climbed to its maximum at 4 weeks-6 weeks. In EGb761 treated rats, both numbers of c-jun positive and survival motoneurons were more than that in control group at each time point. CONCLUSION: EGb761 attenuates avulsion-induced motoneurons death, and this effect may be related to up-regulation of c-jun gene in avulsed motoneurons.  相似文献