Detection of Toxoplasma gondii DNA in Fresh and Frozen Semen from Rams in Brazil     

MJG Bezerra  JALO Cruz  ES Kung  PPF Albuquerque  PCP Kim  EPBX Moraes  JW Pinheiro Júnior  RA Mota 《Reproduction in domestic animals》2014,49(5):753-755

The aim of this study was to estimate the prevalence of genomic DNA of Toxoplasma gondii in semen samples from commercial rams in artificial insemination centres in Brazil, as well as in fresh semen from rams in the northeast of Brazil. In total, 108 semen samples were obtained from artificial insemination centres, and genomic DNA of T. gondii was detected in 24 of 108 (22.2%). The prevalence of antibodies anti‐Toxoplasma gondii among sheep on rural properties was 9.2% (10/109), and 100% of the semen samples of these animals were positive in the PCR for T. gondii DNA. The molecular identity was confirmed through sequencing, which indicated 99.9% similarity with the T. gondii DNA sequences stored in the GenBank. This study reports the first occurrence of T. gondii DNA in the semen of rams, which came from artificial insemination centres in Brazil, as well as the occurrence of T. gondii DNA in the fresh semen of naturally infected rams in the northeast of Brazil.  相似文献   

Traumatic head injury in ruminant livestock     

JW FINNIE 《Australian veterinary journal》1997,75(3):204-208

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Microorganisms three billion years old from the precambrian of South Africa     

Barghoorn ES  Schopf JW 《Science (New York, N.Y.)》1966,152(3723):758-763

A minute, bacterium-like, rod-shaped organism, Eobacterium isolatum, has been found organically and structurally preserved in black chert from the Fig Tree Series (3.1 x 10(9) years old) of South Africa. Filamentous organic structures of probable biological origin, and complex alkanes, which apparently contain small amounts of the isoprenoid hydrocarbons pristane and phytane, are also indigenous to this Early Precambrian sediment. These organic remnants comprise the oldest known evidence of biological organization in the geologic record.  相似文献   

Microfossils     

Schopf TJ 《Science (New York, N.Y.)》1977,196(4297):1433

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Phylum ectoprocta, order cheilostomata: microprobe analysis of calcium, magnesium, strontium, and phosphorus in skeletons   总被引：1，自引：0，他引：1  

T J Schopf  J R Allan 《Science (New York, N.Y.)》1970,169(942):280-282

Calcium, magnesium, and strontium occur in approximately constant ratios in traverses through skeletal walls of a single mineralogy (either calcite or aragonite). Skeletal walls of more than one mineralogy have the magnesium-rich layer (calcite) surrounding the living chamber and the strontium-rich layer (aragonite) on the outside. In contrast, phosphorus may be present in greater or lesser amounts in different parts of the same calcite skeletal wall. Aragonitic crystallites appear oriented roughly perpendicular to skeletal walls, whereas calcitic crystallites are parallel to skeletal walls.  相似文献   

Micropaleontological Studies of Lunar Samples     

Schopf JW 《Science (New York, N.Y.)》1970,167(3918):779-780

Optical and electron microscopic studies of rock chips and dust from the bulk sample box returned by Apollo 11 and of petrographic thin sections and acid-resistant residues of lunar material have yielded no evidence of indigenous biological activity. vestigations.  相似文献   

Microfossils in conophyton from the soviet union and their bearing on precambrian biostratigraphy     

Schopf JW  Sovietov YK 《Science (New York, N.Y.)》1976,193(4248):143-146

Silicified specimens of the Vendian (late Precambrian) "index fossil" Conophyton gaubitza from South Kazakstan contain a diverse assemblage of well-preserved cyanophytic and apparently eukaryotic algae, the first stromatolitic microbiota to be reported from the Soviet Union. Unlike the stromatolites in which they occur, the microorganisms that apparently built this form of Conophyton did not become extinct at the end of the Precambrian.  相似文献   

Search for organic compounds in the lunar dust from the sea of tranquiblity     

Ponnamperuma C  Kvenvolden K  Chang S  Johnson R  Pollock G  Philpott D  Kaplan I  Smith J  Schopf JW  Gehrke C  Hodgson G  Breger IA  Halpern B  Duffield A  Krauskopf K  Barghoorn E  Holland H  Keil K 《Science (New York, N.Y.)》1970,167(3918):760-762

A sample of lunar dust was examined for organic compounds. Carbon detected in concentrations of 157 micrograms per gram had a delta(13)C per mil (PDB) value of + 20. Treatment with hydrochloric acid yielded hydrocarbons of low molecular weight, suggesting the presence of carbides. The gas chromatogram of the acylated and esterified derivatives of the hydrolyzate was similar to that obtained for the Pueblito de Allende meteorite. There were no detectable amounts of extractable high-molecular-weight alkanes, aromatic hydrocarbons, isoprenoid hydrocarbons, normal alkanes, fatty acids, amino acids, sugars, or nucleic acid bases. Traces of porphyrins were found, perhaps arising from rocket exhaust materials.  相似文献   

Use of ultrasound‐guided autologous bone marrow transfer for treatment of suspensory ligament desmitis in 30 race horses (2003–2010)     

MS Hall  JR Vasey  JW Russell  T Russell 《Australian veterinary journal》2013,91(3):102-107

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Preliminary investigation into the prevalence of mucormycosis in the platypus (Ornithorhynchus anatinus) in three catchments in north-west Tasmania     

JW Macgregor  CS Holyoake  SA Munks  ID Robertson  KS Warren 《Australian veterinary journal》2010,88(5):190-196

Objective To investigate the distribution and prevalence of mucormycosis in platypus (Ornithorhynchus anatinus) from the Inglis, Emu and Black-Detention catchment areas in north-west Tasmania. Procedure A field study was performed and resulted in the examination of 44 wild platypuses; in addition, one dead platypus and two live platypuses were examined after they were independently submitted to a local veterinary clinic. Results No cases of mucormycosis were conclusively diagnosed. One platypus with signs consistent with those previously described in cases of mucormycosis was captured in the Emu River catchment. However, laboratory tests did not provide a definitive diagnosis for the lesion. Two platypuses from the Inglis catchment area had signs very similar to those previously described in cases of mucormycosis, but laboratory tests found Corynebacterium ulcerans to be the likely cause of the cutaneous ulcers on one of these platypuses and an unidentified fungal agent to be the cause of a cutaneous nodule in the other. Conclusions These findings do not prove that mucormycosis is absent from the populations studied. However, they may indicate that the prevalence of disease is low. The possibility that Mucor amphibiorum is present in a subclinical form in platypuses, or infecting another reservoir, is not excluded. The findings also suggest that caution should be exercised when diagnosing mucormycosis based on clinical findings alone and raise the possibility that some cases may have been incorrectly diagnosed.  相似文献