Molecular responses differ between sensitive silver carp and tolerant bighead carp and bigmouth buffalo exposed to rotenone     

Amberg JJ  Schreier TM  Gaikowski MP 《Fish physiology and biochemistry》2012,38(5):1379-1391

Some species of fish are more tolerant of rotenone, a commonly used non-specific piscicide, than others. This species-specific tolerance to rotenone has been thought to be associated with the uptake and the efficiency at which the chemical is detoxified. However, rotenone stimulates oxidative stress and superoxides, which are also toxic. Understanding the modes in which fish physiologically respond to rotenone is important in developing improved protocols for its application in controlling aquatic nuisance species. Using a molecular approach, we investigated the physiological and molecular mechanisms of rotenone resistance. Species-specific responses were observed when rotenone-sensitive silver, Hypophthalmichthys molitrix, and both rotenone-resistant bighead carp, Hypophthalmichthys nobilis, and bigmouth buffalo, Ictiobus cyprinellus, were exposed to rotenone. Rotenone levels in plasma were highest 90?min after exposure in both silver carp and bigmouth buffalo, but bigmouth buffalo tolerated over twice the burden (ng?mL(-1)?g(-1)) than silver carp. Expression of genes related with detoxification (cyp1a and gst) increased in silver carp, but either decreased or remained the same in bighead carp. Genes linked with oxidative stress in the cytosol (gpx, cat and sod1) and hsp70 increased only in silver carp after a 6-h exposure. Expression of genes associated with oxidative stress in the mitochondria (sod2 and ucp2) differed between silver carp and bighead carp. Expression of sod2 changed minimally in bighead carp, but expression of ucp2 linearly increased to nearly 85-fold of the level prior to exposure. Expression of sod2 and ucp2 did not change until 6?h in silver carp. Use of sod1 and sod2 to combat oxidative stress results in hydrogen peroxide production, while use of ucp2 produces nitric oxide, a chemical known to inhibit apoptosis. We conclude that the mechanism at which a fish handles oxidative stress plays an important role in the tolerance to rotenone.  相似文献   

Cervicothoracic vertebral subluxation causing ataxia in sheep     

WJ HARTLEY  JMK STEVENSON  PH McCARTHY 《Australian veterinary journal》1994,71(8):267-268

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Zur Tumorbildung bei Mäusen des Inzuchtstammes NMRI nach Injektion von Rinderleukosematerial und Versuche zur Rückübertragung dieses Maustumors auf Kälber     

Chr. Schreier  A. Tolle  F.-W. Schmidt  J. R. Schimmelpfennig 《Zoonoses and public health》1967,14(7):585-597

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Polymorphism of the Melatonin Receptor Genes and its Relationship with Seasonal Reproduction in the Gulin Ma Goat Breed     

P Lai  BY Zhang  PQ Wang  MX Chu  WJ Song  BJ Cai 《Reproduction in domestic animals》2013,48(5):732-737

Melatonin is thought to be the main molecule that transmits the signal of seasonal change to the neuroendocrine system in seasonal breeding species. Melatonin exerts its effects through specific melatonin receptors, MTNR1A and MTNR1B. In the present study, six native goat breeds in China and one introduced goat breed were analysed to investigate the relationship between the genetic polymorphism of receptor genes and seasonal reproduction. Sequencing results showed that there were five polymorphic mutations in the MTNR1A gene and two in the MTNR1B gene. In the MTNR1A gene, genotypes AA, AB and BB for 424C>T and genotypes CC, CD and DD for 589C>A were observed in these goat breeds. In all six native goat breeds, only genotype AA was detected. In the MTNR1B gene, genotypes EE, EF and FF for 1179G>A and genotypes GG, GH and HH for 1529A>G were detected. However, in Gulin Ma goats, the genotypes EE and HH were not found. Moreover, the base of G at position 1179 and A at position 1529 were linked (By Arlequin ver 3.1, Zoological Institute, Berne, Switzerland, http://cmpg.unibe.ch/software/arlequin3 ,D′ = 0.7496, r² = 0.4421, χ² = 489.8679, p = 0.000). Among these mutations, no amino acid change was found in MTNR1A, while both of the mutations in MTNR1B gene caused amino acid changes of R222H and S339G, respectively. The structural analysis showed that the R222H mutation occurred in the first amino acid residue of the third cytoplasmic loop, and the S339G mutation was located in the carboxyl terminus of the protein. In terms of seasonal breeding, all the genotypes we detected showed a similar kidding frequency distribution trend with a higher frequency in May–August than in January–April and in September–December. This suggests that the relationship between the polymorphisms in the MTNR1A and MTNR1B genes and seasonal breeding could not be established.  相似文献   

Understanding imperfect detection in a San Francisco Estuary long‐term larval and juvenile fish monitoring programme          下载免费PDF全文

B. Mahardja  M. J. Young  B. Schreier  T. Sommer 《Fisheries Management and Ecology》2017,24(6):488-503

Imperfect detection can present a significant challenge when monitoring for a rare and imperilled species. Here, a long‐term larval and early‐juvenile fish monitoring programme in the upper San Francisco Estuary was examined to evaluate its overall reliability in detecting various fish species, including the imperilled delta smelt, Hypomesus transpacificus McAllister, for which the programme was designed. Using occupancy modelling, detection probability of species with pelagic larval or juvenile life stages was found to be generally high (≥.95) based on the current sampling effort of three larval net tows per site. However, detection probability can vary considerably from year to year depending on the species’ level of larval production. Water temperature and turbidity were identified as important predictors of occurrence for young‐of‐year delta smelt, longfin smelt Spirinchus thaleichthys (Ayres) and striped bass Morone saxatilis (Walbaum), and there was evidence for fish size selectivity by the sampling gear in all three species. These results highlight the need to consider adaptively managing detection probability by increasing sampling effort in years when young‐of‐year delta smelt abundance is expected to be low, especially when information on the species’ occurrence at a particular region is critical.  相似文献