Immune characterization of long pentraxin 3 in pigs infected with influenza virus     

Elisa Crisci  Lorenzo Fraile  Sonia Valentino  Laura Martínez-Guinó  Barbara Bottazzi  Alberto Mantovani  Maria Montoya 《Veterinary microbiology》2014

Long pentraxin 3 (PTX3) is a conserved pattern-recognition secreted protein and a host-defence-related component of the humoral innate immune system. The aim of the present study was to characterize swine PTX3 (SwPTX3) protein expression in influenza virus infected pigs. First, we performed in silico studies to evaluate the cross-reactivity of PTX3 human antibodies against SwPTX3. Secondly, we used in vitro analysis to detect SwPTX3 presence in swine bone marrow dendritic cells (SwBMDC) upon stimulation with different agents by Western blot and immunofluorescence. Finally, the levels of SwPTX3 were assessed in experimental infection of pigs with different strains of influenza virus. This is a novel study where the expression of SwPTX3 was evaluated in the context of a pathogen infection. The initial characterization of SwPTX3 in influenza virus infected pigs contributes to understand the role of PTX proteins in the immune response.  相似文献   

Deadly Outbreak of Iron Storage Disease (ISD) in Italian Birds of the Family Turdidae     

Silvia PAVONE  Sonia SALAMIDA  Ivan PECORELLI  Elisabetta ROSSI  Elisabetta MANUALI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(9):1209-1212

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Bio-amelioration of alkali soils through agroforestry systems in central Indo-Gangetic plains of India     

Y.P. Singh  Gurbachan Singh  D.K. Sharma 《林业研究》2014,25(4):887-896

A long-term field study was initiated during 1995 at Central Soil Salinity Research Institute, Regional Research Station, Lucknow(26°47′58′′ N and 80°46′24′′ E) to analyze the effect of agroforestry systems on amelioration of alkali soils. Three agroforestry systems(pastoral, silvipastoral and silvicultural) were compared with the control where no agroforestry system was introduced. Tree-based silvicultural and silvipastoral systems were characterized by tree species Prosopis juliflora and Acacia nilotica along with grass species Leptochloa fusca, Panicum maximum, Trifolium alexandrium and Chloris gayana. Growth of ten-year-old Prosopis juliflora and Acacia nilotica planted in combination with grasses was significantly higher over the silviculture system with the same species. Tree biomass yields of P. juliflora(77.20 t?ha-1) and A. nilotica(63.20 t?ha-1) planted under silvipastoral system were significantly higher than the sole plantation of(64.50 t?ha-1 and 52.75 t?ha-1). Fodder yield under the pastoral system was significantly higher than the silvipastoral system during initial years but it was at par with that of silvipastoral systems after eight years of plantation. The microbial biomass carbon in the soils of silvipastoral systems was significantly higher than in soils under sole plantation of trees and control systems. The Prosopis-based silvipastoral system proved more effective in reducing soil pH, displacing Na+ from the exchange complex, increasing organic carbon and available N, P and K. Improvement in soil physical properties such as bulk density, porosity, soil moisture and infiltration rate was higher in the Prosopis-based silvipastoral system than in the silviculture system or control. On the basis of biomass production and improvement in soil health due to tree + grass systems, silvipastoral agroforestry system could be adopted for sustainable reclamation of highly alkali soils.  相似文献   

A model of seasonal foliage dynamics of the subtropical mangrove species Rhizophora stylosa Griff.growing at the northern limit of its distribution     

Sahadev Sharma  A T M Rafiqul Hoque  Kangkuso Analuddin  Akio Hagihara 《中国林学(英文版)》2014,1(3):147-157

Background： Progress of forest production in response to the environment requires a quantitative understanding of leaf area development. Therefore, it is necessary to investigate the dynamics of seasonal crown foliage in order to understand the productivity of mangroves, which play an important role in the subtropical and tropical coastlines of the world. Method： Crown foliage dynamics of the mangrove Rhizophora styloso were studies to reveal patterns of leaf recruitment, survival and seasonal leaf area growth. Results： Flushing of leaves occurred throughout the year, but both flushing and leaf area growth pattern of leaves varied with season. Maximum flushing occurred in summer, but leaf areas did not differ significantly with season. The half-expansion period is longer, and the intrinsic rate of increase was lower in winter. Summer flushed leaves grew faster at their initial stage and reached their maximum area over a shorter period of time. The difference in temperature and air vapor pressure deficit （VPD） between summer and winter contributed to the present dynamics of foliage patterns. The mean leaf longevity was estimated to be 13.1 month. The crown foliage area was almost stable throughout the year. Conclusions： Homeostatic control of the crown foliage area may be accompanied by the existence of ecophysiological mechanisms in R. stylosa. Integrating crown foliage dynamics into forest models represents an important step towards incorporating physiological mechanisms into the models for predicting growth responses to environmental changes and for understanding the complex responses of tree growth and litter production.  相似文献   

Water quality affects SDH activity, protein content and RNA:DNA ratios in fish (Catla catla, Labeo rohita and Oreochromis mossambicus) raised in ponds of a sewage-fed fish farm     

Sonia Mukherjee 《Aquaculture (Amsterdam, Netherlands)》2007,262(1):105-119

The spatial and seasonal variability of the respiratory enzyme succinate dehydrogenase and the protein content were examined in different tissues of fish cultured in three ponds along the effluent gradient of a sewage-fed fish farm. Indian major carp Catla catla (150-230 g) and Labeo rohita (60-190 g) cultured in both the middle and last points of the sewage effluent (stocking pond 1) and (stocking pond 4) and Oreochromis mossambicus (50-160 g), a naturally growing fish of the inlet (facultative pond) and the out let of the sewage effluent (stocking pond 4) were procured every month during the period of January-December, 2005 and were subjected to determination of succinate dehydrogenase activity, total protein, DNA and RNA contents from gill, liver and muscle tissue respectively. The SDH activity of all three test fishes (Catla catla, Labeo rohita and Oreochromis mossambicus) was reduced significantly (ANOVA; P < 0.05) when cultured in SP-4 compared to SP-1 in the case of Catla catla and Labeo rohita and in facultative pond in the case of Oreochromis mossambicus.Conspicuous differences in the SDH activity of fish between the last and first stocking pond or the facultative pond were clearly due to the result of the differences in water quality. There was a direct relationship between SDH activity in gill tissue of any of the fish investigated and ammonia-N concentration of water or water pH. This shows that the respiratory activity of these fishes was strongly affected by the ammonia and pH of water. In other words, this suggests that as the distance from the point source increases, there was a substantial improvement of water quality in the ponds located along the sewage effluent gradient. Evidently, there is a progressive pattern of growth, survival and physiological health of fish and abundance of favorable diversity of food organisms with rich biodiversity.  相似文献   

Assessment of phytochemical and genetic diversity analysis of Plumbago zeylanica L. accessions     

Roy  Arpita  Sharma  Neelam  Bharadvaja  Navneeta 《Genetic Resources and Crop Evolution》2022,69(1):209-219

Genetic Resources and Crop Evolution - Selection of elite accessions is important to get the maximum quantity of bioactive compounds in medicinal plants. In this study, thirteen accessions...  相似文献   

Molecular markers based genetic relatedness studies in tissue culture propagated japanese plum cultivars Santa Rosa and Frontier     

Thakur  Manisha  Luharch  Rakshandha  Sharma  Vishal  Sharma  Dharam Paul 《Genetic Resources and Crop Evolution》2022,69(2):567-575

Genetic Resources and Crop Evolution - Santa Rosa and Frontier are the major Japanese plum (Prunus salicina Lindl.) cultivars grown throughout the world. The present investigation was performed to...  相似文献   

Process Optimization for the Development of Nutritionally Enhanced Nuggets using Ficus geniculata: A Nutritional Approach     

Kumari  Madhu  Kumar  Vikas  Kaur  Ramandeep  Kumar  Satish  Sharma  Rakesh 《Plant foods for human nutrition (Dordrecht, Netherlands)》2022,77(2):241-249

Plant Foods for Human Nutrition - Ficus geniculata (FG) is one of the underutilized fig species in India and throughout the world. However, the different parts of the plant have numerous...  相似文献   

Energy input–output modeling and sensitivity analysis for on-farm mechanically paddy straw bales formation     

Sharma  Ankit  Brar  Amandeep Singh 《Paddy and Water Environment》2022,20(2):255-264

Paddy and Water Environment - The paddy straw was collected in the form of bales with three mechanical process CI (stubble shaver + rectangular baler), CII (stubble...  相似文献   

Use of corn height to improve the relationship between active optical sensor readings and yield estimates     

L. K. Sharma  D. W. Franzen 《Precision Agriculture》2014,15(3):331-345

Early in-season loss of N continues to be a problem in corn (Zea mays L.). One method to improve N use efficiency is fertilizing based on in-season crop foliage sensors. The objective of this study was to evaluate two ground-based, active-optical (GBAO) sensors and explore the use of corn height with sensor readings for improving relationships with corn yield. Two GBAO sensors (GreenSeeker^® (GS), Trimble, Sunnydale, CA, USA; and Holland Crop Circle (CC) ACS 470 Sensor^®, Holland Scientific, Lincoln, NE, USA) were used within 30 established corn N-rate trials in North Dakota at the V6 and V12 growth stages in 2011 and 2012. Corn height was recorded manually at the date of sensor data collection. At the V6 growth stage, the GS relationship to yield and the INSEY (in-season estimate of yield) value was improved when the sensor reading was multiplied times corn height. At the V12 stage, using the GS, the INSEY relationship with yield was also generally increased when height was considered. The CC-based red/near-infrared INSEY relationship with yield was similar to the GS INSEY. The CC-based red edge/near infrared INSEY relationship was increased with height only at the first sensor date, but not with the second. The second CC-based sensor–INSEY relationship with yield was maximized using sensor reading only. Segregating the 30 site data set into sites with high clay surface textures and sites with medium texture improved all INSEY relationships compared to pooling all sites. Relationships between INSEY and corn yield at no-till sites were significant at the V12 stage in the wetter 2011 growing season, but not at the V6 stage either year, nor at the V12 stage in the very dry 2012 season. In the high clay and medium textured soils at the V6 stage, corn height improved the relationship between INSEY and yield often enough to suggest that incorporating corn height into an algorithm for yield prediction would strengthen yield prediction, and thus improve N rate decisions.  相似文献