A quarter century of potato variety trials in Rhode Island     

J. E. Sheehan  T. E. Odland 《American Journal of Potato Research》1956,33(5):161-165

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Response of Thawed Epidi dymal Red Deer Spermatozoa to Increasing Concentrations of Hydrogen Peroxide,and Importance of Individual Male Variability     

AE Domínguez‐Rebolledo  F Martínez‐Pastor  AF Bisbal  JL Ros‐Santaella  O García‐Álvarez  A Maroto‐Morales  AJ Soler  JJ Garde  MR Fernández‐Santos 《Reproduction in domestic animals》2011,46(3):393-403

Oxidative stress represents a challenge during sperm manipulation. We have tested the effect of increasing hydrogen peroxide (H₂O₂) levels on red deer spermatozoa after cryopreservation, and the role of male‐to‐male variation in that response. In a first experiment, eight thawed samples were submitted to 0, 25, 50, 100 and 200 μm H₂O₂ for 2 h at 37°C. Intracellular reactive oxygen species (H₂DCFDA‐CM) increased with H₂O₂ concentration, but we only detected a decrease in sperm function (motility by CASA and chromatin damage by sperm chromatin structure assay) with 200 μm . Lipoperoxidation assessed by the thiobarbituric acid reactive substance (TBARS) method increased slightly with 50 μm H₂O₂ and above. In a second experiment, samples from seven males were submitted to 0 and 200 μm H₂O₂ for 2 h, triplicating the experiment within each male. Males differed at thawing and regarding their response to incubation and H₂O₂ presence. We found that the kinematic parameters reflected male‐to‐male variability, whereas the response of the different males was similar for lipid peroxidation and viability. A multiparametric analysis showed that males grouped differently if samples were assessed after thawing, after incubation without H₂O₂ or after incubation with H₂O₂. Red deer spermatozoa are relatively resilient to H₂O₂ after thawing, but it seems to be a great male‐to‐male variability regarding the response to oxidative stress. The acknowledgement of this individual variability might improve the development of optimized sperm work protocols.  相似文献   

Measurement of cereal α-amylase: A new assay procedure     

Barry V. McCleary  Helen Sheehan 《Journal of Cereal Science》1987,6(3)

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Laboratory Handbook on Bovine Mastitis.     

AF Hope 《Australian veterinary journal》2000,78(7):488-488

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The Comet assay for detection of DNA damage in canine sperm          下载免费PDF全文

AF Pereira  P Borges  A Fontbonne  L Cardoso  I Gaivão  A Martins‐Bessa 《Reproduction in domestic animals》2017,52(6):1149-1152

Sperm DNA integrity is a fundamental prerequisite in fertilization and embryo development. Among DNA integrity tests, the Comet assay is an accurate and sensitive test for the detection of sperm oxidative damage. The aim of this work was to evaluate sperm oxidative damage using the Comet assay and to study the correlation between Comet and routine assays for the evaluation of semen quality. Dogs were divided in two groups: group A (n = 6), comprising dogs with abnormal spermiogram, that is astheno‐, terato‐ or oligoasthenoteratozoospermic (OAT); and group B (n = 8), comprising normospermic dogs. The distribution of sperm oxidative damage was significantly different between the two groups (p = .001): group A—median: 31.55%, interquartile range (IQR): 30.18–38.01; group B—median: 0.90%, IQR: 0.65–1.96. The correlation between oxidative damage and abnormal morphology was high (r = .846; p < .001). There was a negative correlation between progressive motility and oxidative damage (r = ?.792; p = .001). Basal and oxidative DNA damage of spermatozoa are increased in dogs with non‐normospermic semen. In conclusion, and considering the elevated correlation with classical tests of sperm quality, the Comet assay has ample potential for clinical and research purposes in dogs.  相似文献   

BOOK REVIEW     

AF Clarke 《Australian veterinary journal》2003,81(8):487-487

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Pneumonia and gastritis in a cat caused by feline herpesvirus-1     

Glenna F. McGregor  Karen Sheehan  Elemir Simko 《The Canadian veterinary journal. La revue veterinaire canadienne》2016,57(2):147-150

We report a case of fatal respiratory and gastric herpesvirus infection in a vaccinated, adult cat with no known immunosuppression or debilitation. The disease was characterized by severe necrotizing bronchopneumonia, fibrinonecrotic laryngotracheitis, and multifocal necrotizing gastritis associated with eosinophilic intranuclear inclusion bodies and a large amount of feline herpesvirus-1 antigen detected with immunohistochemistry.  相似文献   

Biological diversity     

Jablonski D  Sepkoski JJ  Bottjer DJ  Sheehan PM 《Science (New York, N.Y.)》1984,224(4655):1294

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dSarm/Sarm1 is required for activation of an injury-induced axon death pathway     

Osterloh JM  Yang J  Rooney TM  Fox AN  Adalbert R  Powell EH  Sheehan AE  Avery MA  Hackett R  Logan MA  MacDonald JM  Ziegenfuss JS  Milde S  Hou YJ  Nathan C  Ding A  Brown RH  Conforti L  Coleman M  Tessier-Lavigne M  Züchner S  Freeman MR 《Science (New York, N.Y.)》2012,337(6093):481-484

Axonal and synaptic degeneration is a hallmark of peripheral neuropathy, brain injury, and neurodegenerative disease. Axonal degeneration has been proposed to be mediated by an active autodestruction program, akin to apoptotic cell death; however, loss-of-function mutations capable of potently blocking axon self-destruction have not been described. Here, we show that loss of the Drosophila Toll receptor adaptor dSarm (sterile α/Armadillo/Toll-Interleukin receptor homology domain protein) cell-autonomously suppresses Wallerian degeneration for weeks after axotomy. Severed mouse Sarm1 null axons exhibit remarkable long-term survival both in vivo and in vitro, indicating that Sarm1 prodegenerative signaling is conserved in mammals. Our results provide direct evidence that axons actively promote their own destruction after injury and identify dSarm/Sarm1 as a member of an ancient axon death signaling pathway.  相似文献   

AVA at the frontline     

Sheehan D 《Australian veterinary journal》2008,86(3):N6, N8

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