植物间种强化根际修复PCB NO.14污染土壤的研究     

刘龙元贺鸿志  申时立黎华寿 《中国农学通报》2012,28(36):128-134

为了更好地了解间作系统对受持久性有机污染物污染的农业土壤的生物修复效率及其机制,运用室内盆栽试验,研究了南瓜(Cucurbita moschata)、大豆[Glycine max (L.) Merr.]和玉米(Zea mays L.)3种植物的单作和两两间作强化多氯联苯污染赤红壤的根际修复效应。结果表明：植物不同种植方式均对根际土壤中PCB的减量有促进作用,单作的土壤PCB单位残留量是对照的70.25%~92.11%,间作的土壤PCB单位残留量是对照的61.44%~80.53%。其中,大豆、玉米间作效果最好,根际修复土壤PCB单位残留量是对照的61.44%,显著低于对照。各种植方式中植物地上部吸收积累的多氯联苯符合中国食品安全标准的限值。各处理根际土壤的荧光素二乙酸酯酶、脱氢酶、过氧化氢酶活性和Eh、C/N均与对照差异显著。相关分析结果表明,根际土壤PCB残留量与FDA酶、过氧化氢酶活性、C/N呈极显著负相关,与多酚氧化酶、Eh呈显著负相关。  相似文献   

畜炎宁治疗奶牛子宫内膜炎疗效试验   总被引：1，自引：1，他引：0  

夏长军  赫明雷  宫大庆  高敬华  邢桂珍  廖建维  倪宏波 《黑龙江八一农垦大学学报》2012,24(2):52-57

奶牛子宫内膜炎是奶牛产后主要由于细菌感染而引起的常见产科慢性、常发性疾病,给奶牛养殖业造成很大的经济损失。采集患子宫内膜炎奶牛产后阴道分泌物进行细菌分离鉴定和药敏试验,了解子宫内膜炎病例中病原菌的种类及耐药情况。在临床试验中,选用海正畜炎宁与野菊花、金银花、蒲公英等为主要成分的中草药进行对比疗效试验,通过治疗前后子宫及阴道恢复情况来确定治疗效果。试验结果证实：该牛场引起子宫内膜炎的细菌主要是以大肠杆菌、绿脓杆菌、蜡样芽孢菌、链球菌和枯草芽孢杆菌为主,单一感染所占比例为24．4％,混合感染所占比例为75．6％;药敏试验结果表明海正畜炎宁最为敏感,临床试验也证实：肌肉注射畜炎宁组有效率最高,达到78．75％;子宫灌注畜炎宁组达到75％;而对照组子宫灌注组仅为50％,因此,在治疗牛子宫内膜炎时,海正畜炎宁可作为治疗药物的首选。  相似文献   

猪蓝耳病和传染性胸膜肺炎混合感染的病例报告     

靳明武  张洋龙  孙晓雨  邵光喜  倪宏波 《黑龙江八一农垦大学学报》2012,24(5):32-35

猪繁殖与呼吸障碍综合征是由繁殖与呼吸综合征病毒(PRRSV)引起的一种猪接触性传染病,又称"蓝耳病";而猪传染性胸膜肺炎是由胸膜肺炎放线杆菌引起的一种接触性传染病,他们都是猪的重要呼吸道疾病,在许多养猪国家流行,且经常混合感染,给养殖业造成重大的经济损失。本试验通过对山东某猪场送检病料进行细菌的分离纯化、PCR鉴定等实验室诊断,结果发现其为蓝耳病和传染性胸膜肺炎混合感染。  相似文献   

Identifying and characterizing yield limiting factors in paddy rice using remote sensing yield maps     

Yi-Ping Wang  Shou-Hung Chen  Kuo-Wei Chang  Yuan Shen 《Precision Agriculture》2012,13(5):553-567

Identification and characterization of yield limiting factors based on multi-year yield maps is important for delineating field management zones. Multi-year yield maps were derived from satellite images of a paddy-rice (Oryza sativa L.) study site with a conventional two-cropping system in central Taiwan. Spatiotemporal yield-trend maps with consistently high, average and low yields, and inconsistent yield areas were delineated based on temporal variation and the means of the normalized yields on a per pixel basis. Soil and plant samples were collected and grouped for statistical analysis based on the derived yield-trend maps. Comparison of soil properties and rice yield components among yield classes indicated that differences in leaching loss of basal and top-dressed N fertilizers were the likely limiting factor affecting the spatial variation of yield within the study site.  相似文献   

2010-2012年SCI收录我国肿瘤学论文的文献计量分析     

郑津津  张惠荣  井深  姜珊 《农业环境科学学报》2012,31(12)

对2010-2012年SCIE收录我国肿瘤学论文进行文献计量分析,以了解我国肿瘤学论文存在的问题及今后努力的方向。  相似文献   

RNAi沉默转基因油菜fad2基因表达的种子特异性分析     

周晓婴  申爱娟  张洁夫  浦惠明  龙卫华  胡茂龙  陈锋  付三雄  顾慧  陈松  戚存扣 《分子植物育种》2012,(3):305-310

为研究RNAi沉默转基因油菜fad2基因表达的效果及其器官特异性,实时荧光定量PCR法分析比较了转基因油菜W-4与非转基因对照Westar开花后第7天、第14天、第21天、第28天的种子以及越冬期油菜根、茎、叶器官中的fad2基因的相对表达量。结果显示:对照Westsr种子中fad2基因的相对表达量随开花后的天数呈逐渐增加的趋势,但W-4种子中fad2基因的相对表达量在开花后第21天、第28天较对照Westar显著下降,降幅达到60%。说明转基因油菜W-4在种子发育过程中表达的fad2基因的dsRNA干扰了fad2基因表达。W-4与Westar在越冬期根、茎、叶器官中fad2基因的相对表达量基本一致,无显著性差异。脂肪酸组成分析结果显示:W-4种子中油酸平均含量达到81.5%较Westar增加了近15%,而多不饱和脂肪酸平均含量为5.25%,较Westar下降了近70%;但根、茎、叶中的脂肪酸含量二者无显著差异;结果表明转基因油菜中RNAi干扰fad2基因表达具有种子特异性,不干扰其它器官的fad2基因表达。研究还发现转基因油菜fad2基因表达下降的时期与napin基因表达时期同步,均始于开花后21d左右,表明目标基因的表达受napin启动子的准确控制。  相似文献   

黄瓜ζ-胡萝卜素脱氢酶基因克隆及表达分析     

王柬人  李锡香  王海平  邱杨  宋江萍  张晓辉  沈镝 《分子植物育种》2012,(5):520-527

以西双版纳黄瓜为试材,从黄瓜果肉中克隆ζ-胡萝卜素脱氢酶(ZDS)基因的cDNA序列,命名为CsZds。获得目的片段长度为1805bp,该序列具有完整的开放阅读框,位于31~1761bp,编码576个氨基酸。序列比对分析结果显示,该蛋白在氨基端变化十分明显,中部较为保守,存在一个特征序列。系统进化分析表明,推测的CsZDS蛋白与南瓜中ZDS蛋白同源性最高,达到91.84%。利用实时荧光定量PCR技术分析结果显示,随着果实成熟期的延长,西双版纳黄瓜CsZds基因的表达量呈明显上升的趋势,在转色期达到最大。在果实发育过程中,西双版纳黄瓜CsZds基因的表达量高于普通黄瓜。  相似文献   

Effects of increasing tryptophan intake on growth and physiological changes in nursery pigs     

Shen YB  Voilqué G  Kim JD  Odle J  Kim SW 《Journal of animal science》2012,90(7):2264-2275

Tryptophan (Trp) as a precursor of serotonin (5-hydroxytryptamine, 5-HT) has long been used to extenuate aggressive behavior and control stress of humans as well as several farm animals. This study was conducted to determine the effect of supplemental L-Tryptophan (L-Trp) on growth, cerebral 5-HT concentration, stress hormone concentration, oxidative stress status, and behavior response of pigs under social stress, and also to determine an optimal daily total Trp intake that would benefit nursery pigs under social stress. Seventy two individually housed barrows at 6 wk of age were randomly allotted to 6 treatments with supplementation of 0, 2, 4, 6, 8, or 10 g L-Trp/kg to corn and soybean meal-based feedstuffs. Pigs were fed assigned feedstuffs for 15 d. Body weight was measured on d 0, 5, 10, and 15. Saliva and blood were collected on d 5, 10, and 15. On d 5 and 10, all 12 pigs in each treatment were paired in 6 new pens to record behavior for a 2-d period and returned to original individual pens. On d 15, pigs were euthanized to obtain hypothalamus. During the first 5 d, ADG and G:F increased (linear, P < 0.01) with increasing supplemental L-Trp. During the entire 15 d, ADG and G:F increased (linear, P = 0.01 and P < 0.01, respectively) with increasing supplemental L-Trp. Estimates of the daily total Trp intake based on ADG on d 15 were 10.8 g/d (P < 0.01; R(2) = 0.16) using a 1-slope broken-line analysis. Hypothalamic 5-HT and 5-hydroxyindoleacetic acid increased (linear, P < 0.01 and P = 0.03, respectively) with increasing supplemental L-Trp. Malonedialdehyde in plasma and hypothalamus, as well as salivary cortisol, on d 15 decreased (linear, P = 0.01, P < 0.01, and P < 0.01, respectively) with increasing supplemental L-Trp. Plasma urea nitrogen decreased (linear, P < 0.01) with increasing supplemental L-Trp. Increasing supplemental L-Trp affected pig behaviors during the first 2-d observation period by decreasing (quadratic, P = 0.04) lying, decreasing (linear, P = 0.04) sitting, and increasing (linear, P = 0.02) drinking. Overall, supplementation of L-Trp improved growth performance of 6 wk-old nursery pigs under social stress in association with increasing hypothalamic 5-HT production, reducing stress hormone concentrations, decreasing lipid peroxidation, increasing drinking, and reducing sitting and lying. Increase in BW gain of nursery pigs under social stress was maximal when daily total Trp intake was 10.8 g.  相似文献   

Effect of the diclazuril on Hsp90 in the second-generation merozoites of Eimeria tenella     

Shen X  Wang C  Zhu Q  Li T  Yu L  Zheng W  Fei C  Qiu M  Xue F 《Veterinary parasitology》2012,185(2-4):290-295

  相似文献   

纳米ZnO处理对毛竹材防霉和阻燃性能的影响     

杨优优  鲍滨福  沈哲红 《竹子研究汇刊》2012,(1):10-14

在室温条件下,分别采用0.2%,1%,2%,4%浓度的纳米ZnO对毛竹试件进行处理,比较了纳米ZnO处理前后毛竹防霉性能和阻燃性能的差异。结果表明:当药剂浓度从0%提高到2%时,毛竹试件的防霉效果随纳米ZnO吸药量的增加而提高,当药剂浓度从2%提高到4%时,纳米ZnO吸药量的增加并未提高毛竹试件防霉效果;纳米ZnO处理使毛竹的霉变时间推迟2～3周,并提高了毛竹的阻燃性能。  相似文献