Deletion in chromosome 11p associated with a hepatitis B integration site in hepatocellular carcinoma   总被引：25，自引：0，他引：25  

C E Rogler  M Sherman  C Y Su  D A Shafritz  J Summers  T B Shows  A Henderson  M Kew 《Science (New York, N.Y.)》1985,230(4723):319-322

Hepatitis B virus (HBV), a virus with known carcinogenic potential, integrates into cellular DNA during long-term persistent infection in man. Hepatocellular carcinomas isolated from viral carriers often contain clonally propagated viral DNA integrations. As small chromosomal deletions are associated with several types of carcinomas, the occurrence of chromosomal deletions in association with HBV integration in hepatocellular carcinoma was studied. HBV integration was accompanied by a deletion of at least 13.5 kilobases of cellular sequences in a human hepatocellular carcinoma. The viral DNA integration and deletion of cellular sequences occurred on the short arm of chromosome 11 at location 11p13-11p14. The cellular sequences that were deleted at the site of HBV integration were lost from the tumor cells, leaving only a single copy of the remaining cellular allele.  相似文献   

Safety evaluation and treatment affect of LY2190416, a CB-1 antagonist/inverse agonist in growing beagle dogs     

Nunamaker E  Newhall K  Thompson C  Lucas A  Owens J  Sherman JG 《Journal of veterinary pharmacology and therapeutics》2011,34(6):577-582

The objective of this study was to assess the safe use of LY2190416, a cannabinoid receptor 1 receptor antagonist/inverse agonist, for obesity management in dogs. Twenty-four clinically normal young beagle dogs were administered LY2190416 at doses of 3, 9, or 18 mg/kg or placebo, orally, once daily for 13 weeks. Food consumption and body weight were determined, and dogs were evaluated for changes in hematology, clinical chemistry, urinalysis, and serum cortisol. LY2190416 had no significant effect on hematology, clinical chemistry, urinalysis, and serum cortisol. All dogs consumed 100% of their entire daily allowance throughout the study. All dogs gained weight during the study, but treated dogs gained less than control dogs by the end of the study. During the first month, dogs exhibited a dose-dependent decrease in rate of weight gain (19.7 g/day for control dogs vs. 10.6 g/day for the 18 mg/kg dose group). LY2190416 was found to be safe at doses up to 18 mg/kg administered daily for 3 months. Results suggest that LY2190416 decreases rate of weight gain without affecting appetite or causing significant adverse health effects in normal growing dogs. Possible mechanisms for a proposed metabolic effect are discussed.  相似文献   

Understanding the impact of P-glycoprotein mutation on canine health     

Sherman JG 《Veterinary journal (London, England : 1997)》2011,190(1):13-14

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Paired-end mapping reveals extensive structural variation in the human genome     

Korbel JO  Urban AE  Affourtit JP  Godwin B  Grubert F  Simons JF  Kim PM  Palejev D  Carriero NJ  Du L  Taillon BE  Chen Z  Tanzer A  Saunders AC  Chi J  Yang F  Carter NP  Hurles ME  Weissman SM  Harkins TT  Gerstein MB  Egholm M  Snyder M 《Science (New York, N.Y.)》2007,318(5849):420-426

Structural variation of the genome involves kilobase- to megabase-sized deletions, duplications, insertions, inversions, and complex combinations of rearrangements. We introduce high-throughput and massive paired-end mapping (PEM), a large-scale genome-sequencing method to identify structural variants (SVs) approximately 3 kilobases (kb) or larger that combines the rescue and capture of paired ends of 3-kb fragments, massive 454 sequencing, and a computational approach to map DNA reads onto a reference genome. PEM was used to map SVs in an African and in a putatively European individual and identified shared and divergent SVs relative to the reference genome. Overall, we fine-mapped more than 1000 SVs and documented that the number of SVs among humans is much larger than initially hypothesized; many of the SVs potentially affect gene function. The breakpoint junction sequences of more than 200 SVs were determined with a novel pooling strategy and computational analysis. Our analysis provided insights into the mechanisms of SV formation in humans.  相似文献   

Present issues in behavioral ecology: behavioural ecology     

Sherman PW 《Science (New York, N.Y.)》1984,226(4671):161-162

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Hold your horses: impulsivity, deep brain stimulation, and medication in parkinsonism     

Frank MJ  Samanta J  Moustafa AA  Sherman SJ 《Science (New York, N.Y.)》2007,318(5854):1309-1312

Deep brain stimulation (DBS) of the subthalamic nucleus markedly improves the motor symptoms of Parkinson's disease, but causes cognitive side effects such as impulsivity. We showed that DBS selectively interferes with the normal ability to slow down when faced with decision conflict. While on DBS, patients actually sped up their decisions under high-conflict conditions. This form of impulsivity was not affected by dopaminergic medication status. Instead, medication impaired patients' ability to learn from negative decision outcomes. These findings implicate independent mechanisms leading to impulsivity in treated Parkinson's patients and were predicted by a single neurocomputational model of the basal ganglia.  相似文献   

Molecular biology of the H-2 histocompatibility complex   总被引：25，自引：0，他引：25  

R A Flavell  H Allen  L C Burkly  D H Sherman  G L Waneck  G Widera 《Science (New York, N.Y.)》1986,233(4762):437-443

The H-2 histocompatibility complex of the mouse is a multigene family, some members of which are essential for the immune response to foreign antigens. The structure and organization of these genes have been established by molecular cloning, and their regulation and function is being defined by expression of the cloned genes.  相似文献   

Monitoring the time course of cerebral deoxyglucose metabolism by 31P nuclear magnetic resonance spectroscopy     

R K Deuel  G M Yue  W R Sherman  D J Schickner  J J Ackerman 《Science (New York, N.Y.)》1985,228(4705):1329-1331

The phosphorylation of 2-deoxyglucose by the mammalian brain is used as an index of the brain's energy metabolism. The results of phosphorus-31 nuclear magnetic resonance (31P NMR) monitoring of conscious animals in vivo showed rapid phosphorylation of 2-deoxyglucose by brain tissue. The rate of phosphorylation as determined by 31P NMR was consistent with results achieved by tracer methods using carbon-14-labeled 2-deoxyglucose. However, the disappearance of 2-deoxyglucose-6-phosphate was shown to be faster than that reported by tracer studies and occurred without alterations of intracellular pH and energy homeostasis. These results were confirmed by gas chromatography and mass spectroscopy. It is postulated that 2-deoxyglucose may be metabolized in several ways, including dephosphorylation by a hexose phosphatase.  相似文献   

Robotic observations of enhanced carbon biomass and export at 55 degrees during SOFeX     

Bishop JK  Wood TJ  Davis RE  Sherman JT 《Science (New York, N.Y.)》2004,304(5669):417-420

Autonomous floats profiling in high-nitrate low-silicate waters of the Southern Ocean observed carbon biomass variability and carbon exported to depths of 100 m during the 2002 Southern Ocean Iron Experiment (SOFeX) to detect the effects of iron fertilization of surface water there. Control and "in-patch" measurements documented a greater than fourfold enhancement of carbon biomass in the iron-amended waters. Carbon export through 100 m increased two- to sixfold as the patch subducted below a front. The molar ratio of iron added to carbon exported ranged between 10(4) and 10(5). The biomass buildup and export were much higher than expected for iron-amended low-silicate waters.  相似文献   

Molecular and Cellular Characterization of Buffalo Bone Marrow‐Derived Mesenchymal Stem Cells     

NE Gade  MD Pratheesh  A Nath  PK Dubey  Amarpal  B Sharma  G Saikumar  G Taru Sharma 《Reproduction in domestic animals》2013,48(3):358-367

Immune privileged mesenchymal stem cells (MSCs) can differentiate into multiple cell types and possess great potential for human and veterinary regenerative therapies. This study was designed with an objective to isolate, expand and characterize buffalo bone marrow‐derived MSCs (BM‐MSCs) at molecular and cellular level. Buffalo BM‐MSCs were isolated by Ficoll density gradient method and cultured in Dulbecco’s modified Eagle’s medium supplemented with fetal bovine serum (FBS). These cells were characterized through alkaline phosphatase (AP) staining, colony‐forming unit (CFU) assay, mRNA expression analysis (CD 73, CD 90, CD 105, Oct4 and Nanog), immunolocalization along with flow cytometry (Stro 1, CD 73, CD 105, Oct4, Sox2 and Nanog) and in situ hybridization (Oct4 and Sox2). Multilineage differentiation (osteogenic, adipogenic and chondrogenic) was induced in vitro, which was further assessed by specific staining. Buffalo BM‐MSCs have the capacity to form plastic adherent clusters of fibroblast‐like cells and were successfully maintained up to 16^th passage. These cells were AP positive, and further CFU assay confirmed their clonogenic property. RT‐PCR analysis and protein localization study showed that buffalo BM‐MSCs are positive for various cell surface markers and pluripotency markers. Cytoplasmic distribution of mRNA for pluripotency markers in buffalo BM‐MSCs and multilineage differentiation were induced in vitro, which was further assessed by specific staining. To the best of our knowledge, this is the first report of buffalo BM‐MSCs, which suggests that MSCs can be derived and expanded from buffalo bone marrow and can be used after characterization as a novel agent for regenerative therapy.  相似文献