Infertility in a Dog due to Proximal Cytoplasmic Droplets in the Ejaculate: Investigation of the Significance for Sperm Functionality In Vitro     

AI Peña  M Barrio  JJ Becerra  LA Quintela  PG Herradón 《Reproduction in domestic animals》2007,42(5):471-478

A 4-year-old Basque Shepherd male dog was presented for breeding soundness evaluation after the dog failed to impregnate the three bitches he had mated. Clinical examination showed no anomaly of the reproductive system. Semen evaluation showed normal sperm count (640 x 10(6)), 80% had progressively motile spermatozoa, and 96% had morphologically abnormal sperm of which 84% had proximal cytoplasmic droplet and 12% had proximal droplet plus other anomaly. A zona pellucida-binding assay, using canine oocytes derived from frozen-thawed ovaries, was performed in order to investigate the zona-binding ability of dog spermatozoa with proximal cytoplasmic droplets. For the zona pellucida-binding assay, ovaries were thawed and minced in phosphate-buffered saline + 0.4% bovine serum albumin, the oocytes recovered were divided into two groups of 35-40 oocytes to be, respectively, used with the infertile dog and with a control fertile dog. Spermatozoa were capacitated in Canine Capacitating Medium (CCM) at 38.5 degrees C and 5% CO(2) in air for 2 h before oocyte insemination. Groups of five to six oocytes placed in 45 microl droplets of CCM were incubated for 1 h. Afterwards, 5 microl of CCM containing 25,000 spermatozoa were added to each droplet and co-incubated for 2 h before fixation and evaluation of the complexes. After oocyte insemination, sperm motility and viability were evaluated: the sample from the infertile dog had 85% sperm motility with fast and linear progressive movement, and sperm viability of 92%. The sample from the control dog showed 40% sperm motility with fast and highly curvilinear and erratic movement, high degree of sperm agglutination and sperm viability of 32%. For the infertile dog the mean number of bound spermatozoa/oocyte was 0.33 whereas for the control dog it was 1.80. It was concluded that dog sperm with proximal cytoplasmic droplets seem to lack normal capacitating ability in vitro, and consequently, they may have reduced capacity to bind to the zona pellucida of canine oocytes.  相似文献   

Development of a technique for standing hand‐assisted laparoscopic splenectomy in five horses          下载免费PDF全文

LA Gracia‐Calvo  M Martín‐Cuervo  J Jiménez  V Vieítez  D Argüelles  ME Durán  J Ezquerra 《Australian veterinary journal》2015,93(6):183-188

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In Vitro Mechanical Testing of Braided Polyurethane Elastic Fiber and Braided Polyester for Equine Laryngoplasty          下载免费PDF全文

Hadley Willsallen Diplomate ACVS‐LA  Jane Heller PhD  Lauren Kark PhD  Bryan J. Hilbert Diplomate ACVS 《Veterinary surgery : VS》2015,44(2):223-230

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不同蛋白质水平补饲料对早期断奶犊牦牛生产性能和胃肠道发育的影响     

周爱民  王威  王之盛  邹华围  彭全辉  冯宇诚  何晓林  尹满才  拉环 《动物营养学报》2015,(3):918-925

本试验旨在探究不同蛋白质水平补饲料对3月龄早期断奶犊牦牛生产性能、血液指标和胃肠道发育的影响。选取3月龄(90±5)日龄]健康大通母犊牦牛48头,随机分为4组,每组4个重复,每个重复3头。对照组犊牛全放牧,试验组犊牛在放牧基础上分别补饲不同蛋白质水平(粗蛋白质水平分别17%、18%、19%)的3种等能量(增重净能5.6 MJ/kg)饲粮。预试期7 d,正试期60 d。结果表明:1)与对照组相比,补饲能够显著提高犊牦牛的平均日增重,17%、18%和19%补饲组分别提高了28.87%、47.47%和28.61%(P0.05)。2)与对照组相比,补饲显著提高了血清尿素氮浓度(P0.05),17%、18%和19%补饲组分别增加了10.57%、16.64%、21.84%(P0.05)。3)与对照组组相比,补饲料粗蛋白质水平为18%时,显著提高了瘤胃和大肠的绝对重量和相对重量(P0.05),显著提高了十二指肠、盲肠、结肠的绒毛高度以及十二指肠和盲肠的绒毛高度/隐窝深度(P0.05)。因此,补饲能够提高早期断奶母犊牦牛生产性能和促进胃肠道发育,早期断奶母犊牦牛的补饲料的适宜粗蛋白质水为18%。  相似文献   

中国美利奴羊超细型羊毛理化性能研究及灰评估     

李勇  狄江  王琼  拉扎提  陈为江 《中国畜牧兽医》2009,36(1):129-131

测定中国美利奴羊超细型羊毛理化性能,并与主要经济性状进行相关性分析,运用灰色理论评估不同品系羊毛品质。研究结果表明,超细型羊羊毛纤维直径极显著低于其它品系（P<0.01）;弯曲数显著提高（P<0.05）;纤维直径与其它各性状间均不相关（P>0.05）;毛丛长度增加,净毛量随之增加（P<0.05）;弯曲数增加,抗压缩弹性增强（P<0.01）;净毛光泽度、白度、黄度间呈显著正相关（P<0.05）。超细型羊毛综合品质优良,在本品种内品质较高。  相似文献   

Optic neuropathy and retinopathy in closantel toxicosis of sheep and goats     

PA GILL  RW COOK  JG BOULTON  WR KELLY  B VANSELOW  LA REDDACLIFF 《Australian veterinary journal》1999,77(4):259-261

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增产菌对高寒地区燕麦产草量的增产效应     

邹华  贾德志 《草业科学》1999,16(4):14-16

采用增产菌拌种,喷施２种方法的二因素随机区组设计,对燕麦草的增产效应进行了研究,研究结果,增产菌对燕麦的增产效果显著（Ｐ〈０．０１）,最佳拌种量３００ｇ／ｈｍ＾２,最佳喷施量为３００ｋｇ／ｈｍ＾２拌种和喷施增产菌对燕麦的增产效果是相互独立的,拌种与喷施的交互作用不显著（Ｐ〉０．０５）,两者的增产效应是可加效应,最佳处理组合为３００ｇ／ｈｍ＾２拌种量＋３００ｇ／ｈｍ＾２喷施量。  相似文献   

人工模拟飞播牧草对治理“黑土滩”型退化草地的效果调查   总被引：4，自引：1，他引：3  

拉元林  梁珠英 《青海草业》2000,(4)

采用路线定点抽样方法 ,对人工模拟飞播治理“黑土滩”进行了调查 ,结果表明 ,飞播后平均每公顷鲜草产量 991 2 .6kg,比播前提高了 1 1 .5 5倍 ;植被盖度达 70 %～ 80 % ;草群结构改变 ,一些已消失了的优良牧草相继出现 ,不食毒杂草大幅度下降 ,治理效果良好。  相似文献   

基于地统计学和GIS的喀斯特烟田土壤肥力综合评价——以贵州省毕节地区为例   总被引：1，自引：1，他引：1  

腊贵晓  刘国顺  曹杰  李祖良  翟欣 《河南农业科学》2012,41(4):63-68

以贵州省典型的喀斯特地区———毕节地区为研究区域,利用地统计学和GIS相结合的方法,对烟田土壤肥力进行综合评价。在确定评价因子隶属函数和权重的基础上,建立土壤肥力综合评价指标(IFI)。通过对土壤肥力综合评价指标进行半方差函数分析和普通克里格法(Kriging)最优内插,绘制了毕节地区烟田土壤肥力综合评价指标的空间分布图。描述性统计结果表明,毕节地区烟田土壤肥力综合评价指标值为0.35～0.92,变异系数为14.25%。半方差函数分析表明,毕节地区烟田土壤肥力综合评价指数的最适模型是指数模型;块基比为30%,表明具有中度空间相关性;烟田土壤肥力综合评价指数在空间分布上总体呈从内到外递减的趋势,其中以0.63～0.72和0.72～0.92范围内的面积较大,分别占到总面积的68.20%和20.63%。由此表明,该研究区域大部分土壤肥力状况良好。  相似文献   

Effect of feeding frequency and feeding time on growth of Penaeus vannamei (Boone)   总被引：2，自引：0，他引：2  

L. ROBERTSON  A. L. LA WRENCE  F. L. CASTILLE 《Aquaculture Research》1993,24(1):1-6

Abstract. An intensive growth trial conducted In pens in an earthen pond indicated that growth of Penaeus vannamei (Boone) increased with feeding frequency and suggested that day feedings produce greater growth than night feedings. The differences in growth due to feeding frequency were significant, instantaneous growth rates (IGR) increasing from 1·62% to 1·66% to 1·71%/day as the number of feedings increased from one to two to four per 24 h. Comparison of the IGR of shrimp fed at night (1·64%/day) to that of shrimp fed during the day (1·68%/day) suggested ( P = 0·0633) that growth was greater for shrimp given day feedings than for shrimp given night feedings. Survival of shrimp was not significantly different due to feeding frequency or due to time of feeding and averaged 74·7 ± 6·1%. Results indicate that P. vannamei cultured under conditions similar to those tested should be fed a minimum of four times daily and suggest that feeding during the day is at least as good as and may be preferable to feeding at night.  相似文献