The effect of sound on animals     

E Stephan 《DTW. Deutsche tier?rztliche Wochenschrift》1988,95(2):83-85

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Bruchpilot promotes active zone assembly, Ca2+ channel clustering, and vesicle release   总被引：1，自引：0，他引：1  

Kittel RJ  Wichmann C  Rasse TM  Fouquet W  Schmidt M  Schmid A  Wagh DA  Pawlu C  Kellner RR  Willig KI  Hell SW  Buchner E  Heckmann M  Sigrist SJ 《Science (New York, N.Y.)》2006,312(5776):1051-1054

The molecular organization of presynaptic active zones during calcium influx-triggered neurotransmitter release is the focus of intense investigation. The Drosophila coiled-coil domain protein Bruchpilot (BRP) was observed in donut-shaped structures centered at active zones of neuromuscular synapses by using subdiffraction resolution STED (stimulated emission depletion) fluorescence microscopy. At brp mutant active zones, electron-dense projections (T-bars) were entirely lost, Ca2+ channels were reduced in density, evoked vesicle release was depressed, and short-term plasticity was altered. BRP-like proteins seem to establish proximity between Ca2+ channels and vesicles to allow efficient transmitter release and patterned synaptic plasticity.  相似文献   

Toll-like receptor triggering of a vitamin D-mediated human antimicrobial response     

Liu PT  Stenger S  Li H  Wenzel L  Tan BH  Krutzik SR  Ochoa MT  Schauber J  Wu K  Meinken C  Kamen DL  Wagner M  Bals R  Steinmeyer A  Zügel U  Gallo RL  Eisenberg D  Hewison M  Hollis BW  Adams JS  Bloom BR  Modlin RL 《Science (New York, N.Y.)》2006,311(5768):1770-1773

In innate immune responses, activation of Toll-like receptors (TLRs) triggers direct antimicrobial activity against intracellular bacteria, which in murine, but not human, monocytes and macrophages is mediated principally by nitric oxide. We report here that TLR activation of human macrophages up-regulated expression of the vitamin D receptor and the vitamin D-1-hydroxylase genes, leading to induction of the antimicrobial peptide cathelicidin and killing of intracellular Mycobacterium tuberculosis. We also observed that sera from African-American individuals, known to have increased susceptibility to tuberculosis, had low 25-hydroxyvitamin D and were inefficient in supporting cathelicidin messenger RNA induction. These data support a link between TLRs and vitamin D-mediated innate immunity and suggest that differences in ability of human populations to produce vitamin D may contribute to susceptibility to microbial infection.  相似文献   

Retrospective evaluation of the results of the microbiological meat examination (MFU) from calves and cows     

Dahl P  Zweifel C  Müller R  Stephan R 《Schweizer Archiv für Tierheilkunde》2006,148(7):349-355

The microbiological meat examination (MFU), consisting of a bacteriological analysis and a testing for antibiotic residues, is one of several additional analyses used for an edibility rating of carcasses made during meat inspection. Reasons for performing a microbiological meat examination and procedures in the laboratory are defined in the Swiss ordinance for meat examination (FUV). The aim of this study was to analyze the data of 313 microbiological meat examinations from calves and 2882 microbiological meat examinations from cows carried out at the Institute for Food Safety and Hygiene during a period of 8 years. Reasons for microbiological meat examinations as reported by the meat inspectors were mainly classified to the category of "inflammation and necroses" (FUV, Annex 4, Pt 1.2; calves: 73%, cows: 48%). As declarations of the age of the pathological-anatomical changes (that influences directly the probability of detection of pathogens) were generally missing, it is not surprising that the compliance between a particular pathological-anatomical change and a specific detection of pathogens is poor (calves: 19%, cows: 18% of all MFU). About 18% (calves) and 45% (cows) of the reasons for microbiological meat examinations did not correspond to one of the reasons mentioned in the ordinance for meat examination. However, according to the data set, some reasons require a microbiological meat examination due to an often-found specific detection of pathogens. Otherwise, a remarkable number of reasons mentioned were missing the link to bacteriological etiology. Moreover, 14% (calf) and 7% (cow) of microbiological meat examinations with the declaration "no pretreatment" as well as 15% (calf) and 11% (cow) of microbiological meat examinations without declaration showed a positive result in the testing for antibiotic residues.  相似文献   

Impact features on Stardust: implications for comet 81P/Wild 2 dust     

Hörz F  Bastien R  Borg J  Bradley JP  Bridges JC  Brownlee DE  Burchell MJ  Chi M  Cintala MJ  Dai ZR  Djouadi Z  Dominguez G  Economou TE  Fairey SA  Floss C  Franchi IA  Graham GA  Green SF  Heck P  Hoppe P  Huth J  Ishii H  Kearsley AT  Kissel J  Leitner J  Leroux H  Marhas K  Messenger K  Schwandt CS  See TH  Snead C  Stadermann FJ  Stephan T  Stroud R  Teslich N  Trigo-Rodríguez JM  Tuzzolino AJ  Troadec D  Tsou P  Warren J  Westphal A  Wozniakiewicz P  Wright I  Zinner E 《Science (New York, N.Y.)》2006,314(5806):1716-1719

Particles emanating from comet 81P/Wild 2 collided with the Stardust spacecraft at 6.1 kilometers per second, producing hypervelocity impact features on the collector surfaces that were returned to Earth. The morphologies of these surprisingly diverse features were created by particles varying from dense mineral grains to loosely bound, polymineralic aggregates ranging from tens of nanometers to hundreds of micrometers in size. The cumulative size distribution of Wild 2 dust is shallower than that of comet Halley, yet steeper than that of comet Grigg-Skjellerup.  相似文献   

Hydrogen isotopes in Eocene river gravels and paleoelevation of the Sierra Nevada     

Mulch A  Graham SA  Chamberlain CP 《Science (New York, N.Y.)》2006,313(5783):87-89

We determine paleoelevation of the Sierra Nevada, California, by tracking the effect of topography on precipitation, as recorded in hydrogen isotopes of kaolinite exposed in gold-bearing river deposits from the Eocene Yuba River. The data, compared with the modern isotopic composition of precipitation, show that about 40 to 50 million years ago the Sierra Nevada stood tall (>/=2200 meters), a result in conflict with proposed young surface uplift by tectonic and climatic forcing but consistent with the Sierra Nevada representing the edge of a pre-Eocene continental plateau.  相似文献   

Crystal structure of a lipid G protein-coupled receptor     

Hanson MA  Roth CB  Jo E  Griffith MT  Scott FL  Reinhart G  Desale H  Clemons B  Cahalan SM  Schuerer SC  Sanna MG  Han GW  Kuhn P  Rosen H  Stevens RC 《Science (New York, N.Y.)》2012,335(6070):851-855

The lyso-phospholipid sphingosine 1-phosphate modulates lymphocyte trafficking, endothelial development and integrity, heart rate, and vascular tone and maturation by activating G protein-coupled sphingosine 1-phosphate receptors. Here, we present the crystal structure of the sphingosine 1-phosphate receptor 1 fused to T4-lysozyme (S1P(1)-T4L) in complex with an antagonist sphingolipid mimic. Extracellular access to the binding pocket is occluded by the amino terminus and extracellular loops of the receptor. Access is gained by ligands entering laterally between helices I and VII within the transmembrane region of the receptor. This structure, along with mutagenesis, agonist structure-activity relationship data, and modeling, provides a detailed view of the molecular recognition and requirement for hydrophobic volume that activates S1P(1), resulting in the modulation of immune and stromal cell responses.  相似文献   

Comparison of multi-detector row computed tomography with echocardiography for assessment of left ventricular function in healthy dogs     

Henjes CR  Hungerbühler S  Bojarski IB  Nolte I  Wefstaedt P 《American journal of veterinary research》2012,73(3):393-403

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Bactericidal properties of pradofloxacin against veterinary pathogens     

Silley P  Stephan B  Greife HA  Pridmore A 《Veterinary microbiology》2012,157(1-2):106-111

Pradofloxacin is a new veterinary 8-cyano-fluoroquinolone developed for use against bacterial infections in dogs and cats involving both aerobic and anaerobic bacteria. The minimal bactericidal concentrations have been determined against clinical isolates of Staphylococcus pseudintermedius, Staphylococcus aureus, Escherichia coli, Pasteurella multocida, Streptococcus canis, Proteus spp., Fusobacterium spp., Porphyromonas gingivalis and Prevotella species. A subset of these species was selected, and the in vitro rate of kill by pradofloxacin was determined. For 27 of the 30 tested aerobic strains the pradofloxacin MBC was within two doubling dilutions of the MIC. For the remaining strains, the MIC and MBC were within three to four doubling dilutions. Pradofloxacin also demonstrated bactericidal activity against all anaerobic strains, and the MBC was equal to the MIC for four of the strains, within 1 doubling dilution for three strains, within 2 dilutions for a further 3 strains and within 3 dilutions for the remaining five strains. As pradofloxacin concentration was increased, a faster rate of killing was observed; bactericidal effects were seen in all cases at concentrations ≤ 0.25 μg/mL. The bactericidal activity against the anaerobic strains was marked, of particular relevance was the complete absence of regrowth even at 48 h at concentrations as low as 0.125 μg/mL. In conclusion, pradofloxacin exhibits clear bactericidal activity in terms of MBC and kill kinetics against aerobic and anaerobic clinical isolates from dogs and cats at concentrations that are greatly exceeded within the systemic circulation after administration of the recommended therapeutic doses to the target animals. It is expected that such a rapid rate of kill will play a significant role in clinical efficacy. These data demonstrate the complete and rapid killing of anaerobic bacteria by a veterinary 8-cyano-fluoroquinolone.  相似文献   

A microsatellite and morphological assessment of the Russian National cultivated potato collection     

Tatjana Gavrilenko  Olga Antonova  Anna Ovchinnikova  Lubov Novikova  Ekaterina Krylova  Nina Mironenko  Galina Pendinen  Anna Islamshina  Natalia Shvachko  Stephan Kiru  Ludmila Kostina  Olga Afanasenko  David Spooner 《Genetic Resources and Crop Evolution》2010,57(8):1151-1164

The germplasm collections of the Vavilov Institute of Plant Industry, Russia represent the first germplasm collection made for potatoes, now numbering 8,680 accessions. It has tremendous historical and practical importance and a rich history, having been used to document a polyploid series in the cultivated species, to formulate initial taxonomic hypotheses in potato, for studies of interspecific hybridization, and serving as the germplasm base for Russian breeding efforts. Despite its importance and size, there has never been a study of its molecular diversity, and there were many gaps in its passport data. The purpose of the present study is to obtain morphological, ploidy, and microsatellite (SSR) data needed to set up a useful subset of the collection of cultivated potatoes and closely related wild species, and to use this collection to study cultivated potato taxonomy and phylogeny. Through assessments of viability, passport data, and chromosome counts, we selected a subset of 238 cultivated and 54 wild accessions. A morphological and nuclear SSR study of these collections distinguished only three cultivated species: Solanum curtilobum, S. juzepczukii and S. tuberosum, not the many more cultivated potato species of prior taxonomic treatments. The SSR study supports the ideas of S. acaule as one of the parental species for S. curtilobum and S. juzepczukii. The morphological and SSR results are very similar to other recent studies of cultivated species, and show the need to reclassify the collection of cultivated potatoes by modern taxonomic criteria.  相似文献