Lung function and airway cytologic profiles in horses with recurrent airway obstruction maintained in low-dust environments     

Miskovic M  Couëtil LL  Thompson CA 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(5):1060-1066

BACKGROUND: The effects of long-term environmental management on airway obstruction and inflammation in horses with recurrent airway obstruction (RAO) are unknown. HYPOTHESIS: Horses with RAO maintained in low-dust environments have persistent airway obstruction and neutrophilic inflammation. ANIMALS: Study horses were treated for RAO and then maintained in low-dust environments with no medical management. Horses were classified into 3 groups by years after diagnosis: 1 year (time 1, n = 9), 2-3 years (time 2, n = 7), and 5-6 years (time 3, n = 8). The comparison groups were age-matched healthy horses. METHODS: In this cross-sectional study, a clinical examination was performed, and the clinical score was calculated. Standard lung function, forced expiratory maneuvers, and the cytology of bronchoalveolar lavage fluid (BALF) were evaluated. RESULTS: The clinical scores of the RAO horses were higher than those of the non-RAO horses at time 2 (P = .018). Standard lung function data were not different between the groups at any time point. The forced expiratory flow between 75-95% of exhaled vital capacity was lower in RAO horses than in non-RAO horses at all time points (P < .02), indicating persistent peripheral airway obstruction. Cytologic evaluation of BALF revealed no difference in total nucleated cell numbers or differential cell counts between RAO and non-RAO horses at any time point. CONCLUSIONS AND CLINICAL IMPORTANCE: The peripheral airway obstruction detected in horses with RAO maintained in low-dust environments likely is due to irreversible airway remodeling but is not associated with cytologic evidence of airway inflammation.  相似文献   

Environment and the developing world: principles,policies and management,A. Gupta and M. G. Asher. Wiley,Chichester, 1998. ISBN 0 471 96604 5, £50.00 (hardback), 0 471 983387 1 £18.99 (paperback), x+360 pp     

D. A. Thompson 《Land Degradation \u0026amp; Development》2000,11(5):499-500

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Fractionation of High-Amylose Maize Starches by Differential Alcohol Precipitation and Chromatography of the Fractions     

Jeffrey D. Klucinec  Donald B. Thompson 《Cereal Chemistry》1998,75(6):887-896

Three high-amylose maize starches (HAS) and a common corn starch (CCS) were subjected to differential alcohol precipitation using isoamyl alcohol and 1-butanol to obtain fractions designated as amylose (AM), amylopectin (AP), and intermediate material (IM). For each starch, IM had a blue value and an iodine binding wavelength maximum (λ_max) between the λ_max of the respective AM and AP. Size-exclusion chromatography (SEC) showed similarities in the AM from CCS and HAS. HAS AP had higher blue values and iodine binding λ_max values than CCS AP. SEC of the intact HAS AP and IM both showed large proportions of material eluting after the void volume (45–85%) when compared to CCS AP and IM. Chain length (CL) distributions of debranched AP and IM indicated that these fractions from each starch were highly branched, and that AP had a shorter average chain length than IM. Consequently, the differential precipitation behavior of the HAS AP and IM appears dependent on general branching structure rather than size. We conclude that in both CCS and HAS, AP and IM are subsets of the branched molecules with AP as the predominant fraction. For HAS, AP and IM include molecules of a size typical for AM and contain a higher proportion of chains that are longer than those of CCS AP. Differential alcohol precipitation is a useful method of separating amylose, amylopectin, and intermediate material from HAS.  相似文献   

Retrogradation of du wx and su2 wx Maize Starches After Different Gelatinization Heat Treatments     

Qiang Liu  Donald B. Thompson 《Cereal Chemistry》1998,75(6):868-874

Retrogradation of du wx and su2 wx starches after different gelatinization heat treatments was studied by differential scanning calorimetry. Suspensions of 30% (w/w) starch were initially heated to final temperatures of 55–180°C. Gelatinized starch was cooled and stored at 4°C. Starch retrogradation in the storage period was influenced by initial heat treatments. Retrogradation of du wx starch was rapid: when initially heated to 80–105°C, retrogradation enthalpy was ≈10 J/g after one day at 4°C. The retrogradation enthalpy was ≈15 J/g after 22 days of storage, and reached a maximum of 16.2 J/g after 40 days of storage. For du wx starch, application of the Avrami equation to increases in retrogradation enthalpy suggests retrogradation kinetics vary with initial heating temperature. Furthermore, starch retrogradation may not fit simple Avrami theory for initial heating ≤140°C. Retrogradation of su2 wx starch was slow. After 30 days of storage at 4°C, the maximum retrogradation enthalpy for all initial heating temperatures tested was 7.0 J/g, for the initial heating to 80°C. This work indicates that gelatinization heat treatment in these starches is an important factor in amylopectin retrogradation, and that the effect of initial heat treatment varies according to the genotype.  相似文献   

Sheep grazing and defoliation of contrasting varieties of organically grown winter wheat with and without undersowing   总被引：1，自引：0，他引：1  

M. J. Gooding  N. D. Cosser  A. J. Thompson  & W. P. Davies 《Grass and Forage Science》1998,53(1):76-87

Field experiments in Gloucestershire, UK, in the 1990–91, 1991–92, 1993–94 and 1994–95 growing seasons explored the merits of grazing in spring a traditional tall wheat (Triticum aestivum) variety, Maris Widgeon, with more modern shorter varieties. In the first 2 years, defoliation was achieved by mowing at 7 cm in March and/or April. In the second 2 years, varieties sown at two sowing dates were grazed by sheep at a stocking rate of 42 × 50 kg sheep ha^?1 for 3 or 4 days in March. Defoliation reduced crop height and interception of photosynthetically active radiation (PAR). In 1991–92, mowing significantly reduced grain yield of some of the shorter varieties but not of Maris Widgeon. This interaction was related to the amount of PAR intercepted. In this year, mowing improved the establishment of undersown white clover (Trifolium repens) and perennial ryegrass (Lolium perenne), but in subsequent years the conditions were much drier and undersowing failed. In the last two experiments, grazing in March did not significantly reduce grain yield of any variety. The quality of the forage eaten by the sheep had a modified acid-detergent fibre (MADF) content of less than 300 g kg^?1 dry matter (DM) and a crude protein (CP) content of more than 200 g kg^?1 DM in both seasons. Yield of DM and calculated metabolizable energy (ME) of different varieties removed by the sheep interacted strongly with sowing date. September-sown Maris Widgeon provided ≈ 0·7 and 0·3 t DM ha^?1 (or 7·8 and 3·4 GJ ME ha^?1) in March 1994 and March 1995 respectively. However, the shorter wheat varieties, Hereward and Genesis, only provided 0·3 and 0·1 t DM ha^?1 when sown at the same time in the 2 years. At later sowing dates all of the varieties only provided about 0·1 t DM ha^?1 when sown in October 1993, or 0·01 t DM ha^?1 when sown in November 1994. Sheep grazing reduced total weed biomass in June, and reduced the emergence of weed seedlings from soil samples collected after the wheat harvest. Effects of defoliation on foliar infection by Septoria tritici were inconsistent.  相似文献   

The morphology and seasonal changes in cold hardiness, dormancy intensity and root growth potential of rooted cuttings of Sitka spruce   总被引：1，自引：0，他引：1  

Fennessy  J.; O'Reilly  C.; Harper  C.P.; Thompson  D. 《Forestry》2000,73(5):489-497

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Characterization of <Emphasis Type="Italic">Phytophthora infestans</Emphasis> Isolates from Jersey,Channel Islands     

Kenneth L. Deahl  Frances M. Perez  Jonathan M. Thompson  Caoimhe Fleming-Archibald  Stephen Thompson  Rosemary Collier  Steven Kildea  Louise R. Cooke 《Potato Research》2009,52(4):341-354

Potato production on the island of Jersey, in the English Channel, is dominated by Jersey Royal, a selection of the early cultivar International Kidney. Jersey Royal is very susceptible to Phytophthora infestans, the cause of potato late blight, and Jersey’s climate is frequently conducive to infection. During 2004–2006, isolates of P. infestans were obtained from Jersey Royal plant samples from 41 different sites (24 crops, one infected tuber, 16 volunteers) and from five outdoor tomato crops and characterised by mating type, mtDNA haplotype, Gpi and Pep allozyme genotype, metalaxyl resistance and RG57 fingerprint. A subset of 17 isolates was additionally characterised by simple sequence repeat (SSR). All but one isolate from potato belonged to a single A1 multilocus genotype or a variant. The five isolates from tomato represented three distinct genotypes, none of which was found on potato and included one which was A2 mating type. The populations of P. infestans on potato and tomato in Jersey appear distinct, with that on potato being highly clonal.  相似文献   

Detection and identification of aquatic mycobacteria in formalin-fixed, paraffin-embedded fish tissues     

F Pourahmad  K D Thompson  A Adams  R H Richards 《Journal of fish diseases》2009,32(5):409-419

The isolation of mycobacteria from field samples is problematic, and isolation of the bacterium is sometimes not even attempted. The detection of mycobacteria through traditional histology using formalin‐fixed, paraffin‐embedded (FFPE) tissues is neither sensitive nor specific. However, detection of mycobacterial DNA from FFPE specimens, suspected of being infected with mammalian mycobacteriosis, is a routine clinical procedure. In the present study, a polymerase chain reaction (PCR)‐based method was used to detect and identify mycobacteria in FFPE specimens sampled from fish suspected of being infected with fish mycobacteriosis. A total of 45 fish tissue samples, comprising of 12 tissue samples obtained from experimentally infected fish and the remainder from fish naturally infected with mycobacteria, were analysed using a PCR protocol which amplifies a fragment of the mycobacterial 65 kDa heat‐shock protein (hsp65) gene. PCR‐restriction enzyme analysis and/or sequencing were employed to further analyse the PCR amplicons. The PCR results were compared with those obtained by histology and culture. Mycobacterial DNA was detected in 34 of the 45 samples examined, of which 16 samples (47%) showed granulomatous reactions on histological examination. Using histology as the gold standard, no false‐negative PCR results were obtained. Also, considering the presence or absence of granulomas as a diagnostic criterion, the sensitivity and specificity of PCR in 42 of the FFPE tissues were 16/16 (100%) and 8/26 (～30.8%), respectively. Corresponding microbiological cultures were available for 15 cases, of which 13 were pure Mycobacterium cultures. Of these, 13 were PCR positive (100% sensitivity and 50% specificity). The PCR‐based methods used here proved sensitive, specific and rapid for the detection of mycobacteria in routinely processed paraffin wax‐embedded and formalin‐fixed histological samples, and the results of the study suggest that this method has potential use in retrospective epidemiological studies.  相似文献   

Wind speed and crown class influence the height–diameter relationship of lodgepole pine: Nonlinear mixed effects modeling     

Shawn X. Meng  Shongming Huang  Victor J. Lieffers  Thompson Nunifu  Yuqing Yang 《Forest Ecology and Management》2008,256(4):570-577

Wind-induced mechanical perturbation is one of the mechanisms determining the height–diameter relationship of trees, but its effects have largely been neglected in past height–diameter models. In this study, we examined the effects of including wind speed as a regressor in the height–diameter model for lodgepole pine (Pinus contorta var. latifolia Engelm.). We also tested the hypothesis that the height–diameter relationship differs between trees of different crown classes (dominant and subordinate trees), and that a mixed effects height–diameter model developed at the crown class level should achieve a better prediction than that developed at the plot level. The results showed that including wind speed significantly improved the fit of the height–diameter model. The results also showed that the height–diameter relationships differ significantly between the two crown classes. The mixed effects model developed based on crown classes of individual trees had better fit with reduced residual variance and decreased Akaike's information criterion and the Schwarz's Bayesian information criterion than the plot-based mixed model. Evaluation of the developed model suggests the crown class-based height–diameter model has better prediction in terms of prediction error and precision. The developed crown class-based mixed effects model can be used to provide more accurate prediction of tree heights for lodgepole pine from their diameters.  相似文献   

Impacts of rising atmospheric carbon dioxide on model terrestrial ecosystems   总被引：2，自引：0，他引：2  

TH Jones  LJ Thompson  JH Lawton  TM Bezemer  RD Bardgett  TM Blackburn  KD Bruce  PF Cannon  GS Hall  SE Hartley  G Howson  CG Jones  C Kampichler  E Kandeler  DA Ritchie 《Science (New York, N.Y.)》1998,280(5362):441-443

In model terrestrial ecosystems maintained for three plant generations at elevated concentrations of atmospheric carbon dioxide, increases in photosynthetically fixed carbon were allocated below ground, raising concentrations of dissolved organic carbon in soil. These effects were then transmitted up the decomposer food chain. Soil microbial biomass was unaffected, but the composition of soil fungal species changed, with increases in rates of cellulose decomposition. There were also changes in the abundance and species composition of Collembola, fungal-feeding arthropods. These results have implications for long-term feedback processes in soil ecosystems that are subject to rising global atmospheric carbon dioxide concentrations.  相似文献