A survey of pesticide usage against leatherjackets (Tipula species) in south-west scotland in 1975     

Graham G. Tucker  James R. Cutler 《Pest management science》1979,10(3):247-253

A survey of 2000 farms in south-west Scotland revealed that 3.5% of the cereal area had been treated with pesticides for the control of leatherjackets (Tipula spp.) in 1975. A small area of recently established grass and some turnip fields were also treated. DDT and gamma-HCH were the most commonly used compounds with an estimated use respectively of 1863 and 376 kg of active ingredient. Pesticide usage in 1975 appeared to have been consistent with the generally low leatherjacket population in that year.  相似文献   

The production of equine monoclonal immunoglobulins by horse-mouse heterohybridomas.     

C M Richards  H A Aucken  E M Tucker  D Hannant  J A Mumford  J R Powell 《Veterinary immunology and immunopathology》1992,33(1-2):129-143

Studies were carried out to determine the optimum conditions for the production of equine monoclonal antibodies (MAbs). Lymphocytes from ponies immunised with influenza A equine 2 virus, isolate A/Equine/Newmarket/79 (H3N8) were fused with mouse myeloma (NSO) cells and with horse-mouse heterohybridomas made aminopterin-sensitive by selective growth in 8-azaguanine. Although all fusions initially resulted in heterohybridoma colonies that secreted equine immunoglobulin, many of these were unable to maintain secretion for longer than a few weeks. Increasing the time between immunisation and the booster injection of Newmarket/79 virus, the inclusion of Freund's incomplete adjuvant and the use of an aminopterin-sensitive primary heterohybridoma as the fusion partner, improved the production of HIg-secreting heterohybridomas. After two clonings eight cell lines were established which maintained anti-Newmarket/79 antibody secretion for over a year. FACS analysis of the cell lines provided a useful means of predicting breakdown of MAb secretion by the cell lines, thus enabling re-cloning to be carried out in time.  相似文献   

CEREBRAL SINUS VENOGRAPHY IN THE DOG: A NEW TECHNIQUE     

G. Elizabeth  Pluhar  DVM  MS  Russell L.  Tucker  DVM  Patrick R.  Gavin  DVM  PhD  Rodney S.  Bagley  DVM  Mitsuhiko  Takeuchi  DVM 《Veterinary radiology & ultrasound》1997,38(2):112-115

A new of performing cerebral sinus venography was developed that opacivies both the ventral and most of the dorsal venous sinus systems. A pediatric angiographic catheter was introduced into the external jugular vein and advanced to the level of the temporal sinus. Iodinated contrast medium was injected manually and radiographs were made. Subtraction radiography was used to visualize vessels field wit contast medium. Venography was simple and relatively non-invasive and was considered safe. The technique was used to confirm occlusion of the transverse venous sinus in healthy dogs that had undergone radical craniectomies.  相似文献   

The evaluation of isoxsuprine hydrochloride for the treatment of navicular disease: a double blind study   总被引：1，自引：0，他引：1  

A S Turner  C M Tucker 《Equine veterinary journal》1989,21(5):338-341

A randomised double-blind clinical trial of 28 horses was undertaken to evaluate the efficacy of isoxsuprine hydrochloride at four different doses:- 0.0 mg/kg bodyweight (bwt) (placebo), 0.6 mg/kg bwt, 1.2 mg/kg bwt and 1.8 mg/kg bwt for treatment of navicular disease. The results showed that horses treated with isoxsuprine hydrochloride (N = 22) responded significantly with respect to clinical assessment score (P less than 0.01) when compared with the control group (N = 6). Furthermore, there were no dose-related differences in the responses of the horses treated with increasing levels of isoxsuprine. No correlation was found between radiological evidence of the extent of navicular disease and severity of lameness or response to treatment.  相似文献   

Histamine, tyramine, tryptamine and electrolytes during glucose induced lactic acidosis.   总被引：1，自引：0，他引：1  

L N Irwin  G E Mitchell  R E Tucker  G T Schelling 《Journal of animal science》1979,48(2):367-374

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Blood concentration studies in cattle of a sustained-release form of sulfamethazine   总被引：1，自引：0，他引：1  

T Ellison  S F Scheidy  S Tucker  G C Scott  C B Bucy 《Journal of the American Veterinary Medical Association》1967,150(6):629-633

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Anovulation in postpartum suckled beef cows. II. Associations among binding of 125I-labeled gonadotropins to granulosa and thecal cells, and concentrations of steroids in serum and various sized ovarian follicles     

L J Spicer  E M Convey  K Leung  R E Short  H A Tucker 《Journal of animal science》1986,62(3):742-750

To determine if specific binding of 125I-labeled gonadotropins to granulosa and thecal cells, or concentrations of steroids in ovarian follicles change during the postpartum anovulatory period, 21 suckled beef cows were slaughtered on d 7, 14, 28, 42 or 56 after parturition (n = 4 to 6 per d). After slaughter, 10 to 15 follicles were dissected from each pair of ovaries and categorized by diameter: small (1.0 to 3.9 mm), medium (4.0 to 7.9 mm) or large (greater than or equal to 8 mm). Progesterone (221 to 612 ng/ml), androstenedione (48 to 94 ng/ml) and estradiol (2.7 to 23.9 ng/ml) did not change (P greater than .10) in fluid of small or medium follicles from d 7 to 42 to 56 after parturition. Similarly, specific binding of human chorionic gonadotropin (125I-hCG) or follicle stimulating hormone (125I-oFSH) to homogenates of small, medium or large follicles did not change (P greater than .05). In contrast, progesterone in fluid of large follicles increased (P less than .05) 3.4-fold between d 7 and 14, but decreased (P less than .05) 55% between d 14 and 28. Concentrations of androstenedione in fluid of large follicles did not change (P greater than .10) from d 7 to 42 to 56. Concentrations of estradiol in fluid of large follicles remained constant between d 7 and 14, but increased (P less than .05) 4.2-fold between d 14 and 28. We conclude that during the postpartum anovulatory period, there is no change in steroidogenic capabilities of small or medium follicles, both of which predominantly produce progesterone.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Oxygen consumption of a flying bird     

Tucker VA 《Science (New York, N.Y.)》1966,154(3745):150-151

Budgerygahs (Melopsittacus undulatus) flew with a minimum oxygen consumption of 38 milliliters per gramhour at each of three speeds between 19 and 33 kilometers per hour in a recirculating wind tunnel. An oxygen debt accumulated in the first minute of flight and was repaid in the minute following cessation of flight. Frequency of wingbeat was independent of flight speed. The data suggest that flying budgerygahs have a higher cardiac output per kilogram of body weight than mammals and that flight muscle of the budgerygah is among the most metabolically active tissues known.  相似文献   

Assessment of smooth muscle function in Sesbania drummondii toxicosis in Gallus domesticus   总被引：1，自引：0，他引：1  

C S Venugopalan  W Flory  T A Tucker  C D Hebert  G M Strain 《American journal of veterinary research》1984,45(4):764-768

An in vitro assessment based on tissue responsiveness to 2 agonists-histamine and carbachol-was made on smooth muscle activity in chickens experimentally poisoned with sesbania. Crude extracts of Sesbania drummondii were prepared and 2 dosage levels, 0.25% and 0.5% of body weight, were used. The birds were dosed orally with the extract for 3 consecutive days, and on the 4th day segments of ileum and lung were collected from each bird. The isometric contractions of each tissue, produced by the addition of histamine or carbachol in graded concentrations, were recorded. The cumulative concentration-effect curves for the tissues to the agonists were constructed and compared with respective control curves. The results indicated the responsiveness of the tissues in the treated groups was significantly decreased, compared with that of tissues in the controls. Responses of both intestinal and parenchymal strips in the chickens given the higher dosage (0.5%) were decreased significantly, whereas in those given the smaller dosage (0.25%), only parenchyma had a significant response. This indicates that the activity of smooth muscles in general was depressed by sesbania. The effect was more evident in the lung than in the intestine. Therefore, an active principle in the extract which affects smooth muscle, rather than causing direct irritation, may exist. This assessment of smooth muscle activity is sensitive and was effective in detecting changes in tissues from sesbania-treated birds which had not shown any clinical signs. The results also support the possibility that smooth muscle involvement could be a primary cause of toxicity in sesbania poisoning.  相似文献   

Influence of ambient temperature on prolactin concentrations in serum of Holstein and Brahman x Hereford heifers     

R P Wettermann  H A Tucker  T W Beck  D C Meyerhoeffer 《Journal of animal science》1982,55(2):391-394

Four Holstein and four Brahman x Hereford heifers about 8 mo of age were used in a study to determine whether breed influences the effects of ambient temperature on concentrations' of prolactin in serum. Two heifers of each breed were stanchioned in each of two environmental chambers at 21 C for 7 d, after which chamber temperatures were changed to 7 or 31 C during 6 h. After 5 d at 7, 21 or 31 C, heifers were injected with 60 micrograms thyrotropin-releasing hormone (TRH). A switch-back design was used and each heifer was exposed to all treatments. Concentrations of prolactin in serum of heifers during exposure to 7, 21 or 31 C for 5 d were related to ambient temperature (9.0, 20.9 and 29.5 ng/ml, respectively; P less than .001), but the response was not influenced by breed. Heifers of both breeds responded similarly to treatment with TRH, and prolactin in serum increased (P less than .001) within 5 min from 7.0 +/- 3.2 to 45.7 +/- 8.2 ng/ml in heifers at 7 C, from 13.1 +/- 1.6 to 97.2 +/- 9.6 ng/ml in heifers at 21 C and from 18.2 +/- 3.5 to 96.2 +/- 11.3 ng/ml in heifers at 31 C. We conclude that concentrations of prolactin in serum of heifers are positively associated with ambient temperature and that the effects of temperature on basal and TRH-stimulated concentrations of prolactin do not differ significantly between Holstein and Brahman x Hereford heifers. Thus, differences in tolerance to heat were not related to differences in prolactin secretion.  相似文献