Detection of Coxiella burnetii in placenta and abortion samples from British ruminants using real-time PCR     

Jones RM  Twomey DF  Hannon S  Errington J  Pritchard GC  Sawyer J 《The Veterinary record》2010,167(25):965-967

A real-time PCR was developed to detect Coxiella burnetii (the cause of Q fever) in ruminant placentas and aborted fetuses. Primer and probe sets previously developed for human tissue studies were used to target the insertion sequence IS1111 gene for C burnetii. The assay was highly sensitive, with a limit of detection of 10 copies of template, theoretically equating to a single bacterium, and did not cross-react with a panel of other bacteria. To determine sensitivity on field samples submitted for the diagnosis of abortion, results using the IS1111 PCR assay were compared with a com1 PCR assay. When applied to ruminant abortion material, including placental cotyledons and fetal samples, the IS1111 and com1 assays yielded positive results in 23 (25 per cent) of 93 and 19 (20 per cent) of 93 samples, respectively. One infected goat herd was monitored for 31 months: 57 (92 per cent) of 62 placental cotyledon samples from aborting and non-aborting goats, and 10 (30 per cent) of 33 fetal samples were positive by the IS1111 PCR assay.  相似文献   

High-Tc superconductors in the two-dimensional limit:     

JH Choy  SJ Kwon  GS Park 《Science (New York, N.Y.)》1998,280(5369):1589-1592

The free modulation of interlayer distance in a layered high-transition temperature (high-Tc) superconductor is of crucial importance not only for the study of the superconducting mechanism but also for the practical application of high-Tc superconducting materials. Two-dimensional (2D) superconductors were achieved by intercalating a long-chain organic compound into bismuth-based high-Tc cuprates. Although the intercalation of the organic chain increased the interlayer distance remarkably, to tens of angstroms, the superconducting transition temperature of the intercalate was nearly the same as that of the pristine material, suggesting the 2D nature of the high-Tc superconductivity.  相似文献   

Magnetic Field and Plasma Observations at Mars: Initial Results of the Mars Global Surveyor Mission   总被引：2，自引：0，他引：2  

MH Acu?a  JEP Connerney  P Wasilewski  RP Lin  KA Anderson  CW Carlson  J McFadden  DW Curtis  D Mitchell  H Reme  C Mazelle  JA Sauvaud  C d'Uston  A Cros  JL Medale  SJ Bauer  P Cloutier  M Mayhew  D Winterhalter  NF Ness 《Science (New York, N.Y.)》1998,279(5357):1676-1680

The magnetometer and electron reflectometer investigation (MAG/ER) on the Mars Global Surveyor spacecraft has obtained magnetic field and plasma observations throughout the near-Mars environment, from beyond the influence of Mars to just above the surface (at an altitude of approximately 100 kilometers). The solar wind interaction with Mars is in many ways similar to that at Venus and at an active comet, that is, primarily an ionospheric-atmospheric interaction. No significant planetary magnetic field of global scale has been detected to date (<2 x 10(21) Gauss-cubic centimeter), but here the discovery of multiple magnetic anomalies of small spatial scale in the crust of Mars is reported.  相似文献   

Dictionary of Veterinary Epidemiology     

SJ More 《Australian veterinary journal》1999,77(12):813-813

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Pilot study of Australian veterinarians and their perceptions and experiences related to online pet health information     

LR Kogan  JA Oxley  SJ Hazel 《Australian veterinary journal》2019,97(1-2):10-13

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Isolation of Treponema hyodysenteriae from a wild rat living on a piggery     

DJ HAMPSON  BG COMBS  SJ HARDERS  ID CONNAUGHTON  VA FAHY 《Australian veterinary journal》1991,68(9):308-308

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Intestinal dilation in organic layers     

Twomey DF  Wood AM  Young SC 《The Veterinary record》2005,157(2):63-64

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Streptococcus gallolyticus subsp. pasteurianus septicaemia in goslings     

Barnett J  Ainsworth H  Boon JD  Twomey DF 《Veterinary journal (London, England : 1997)》2008,176(2):251-253

Increased mortality was observed in a group of 2-3-week old goslings. At post mortem examination, gross lesions included congestion and haemorrhages in the viscera. Histopathological lesions included generalised congestion of tissues with multifocal bacterial colonies, an acute septic fibrinoid splenitis, and an acute septic hepatitis, all consistent with acute septicaemia. Gram-stained sections of liver and lung confirmed the presence of Gram-positive bacteria and Streptococcus gallolyticus subsp. pasteurianus (Streptococcus bovis biotype II/2) was isolated in pure growth from two of the goslings, and in mixed growth from a third.  相似文献   

The structure and regulation of the Irish equine industries: Links to considerations of equine welfare     

Collins J  Hanlon A  More S  Duggan V 《Irish veterinary journal》2008,61(11):746-756

The equine industries in Ireland are vibrant and growing. They are broadly classified into two sectors: Thoroughbred racing, and sports and leisure. This paper describes these sectors in terms of governance, education and training in equine welfare, and available data concerning horse numbers, identification, traceability and disposal. Animal welfare, and specifically equine welfare, has received increasing attention internationally. There is general acceptance of concepts such as animal needs and persons' responsibilities toward animals in their care, as expressed in the 'Five Freedoms'. As yet, little has been published on standards of equine welfare pertaining to Ireland, or on measures to address welfare issues here. This paper highlights the central role of horse identification and legal registration of ownership to safeguard the health and welfare of horses.  相似文献   

Trends in cow numbers and culling rate in the Irish cattle population, 2003 to 2006     

Maher P  Good M  More S 《Irish veterinary journal》2008,61(7):455-463

Cows are the main economic production units of Ireland's cattle industry. Therefore, demographic information, including overall numbers and survival rates, are relevant to the Irish agricultural industry. However, few data are available on the demographics of cows within a national population, either in Ireland or elsewhere, despite the recent development of comprehensive national cattle databases in many EU Member States. This study has sought: to determine the rate of cow culling from the national herd; to determine the rate of culling by type (dairy, beef), age, method of exit, date of exit and interval between last calving and exit; to calculate the national cow on-farm mortality rate; and to compare the Irish rates with published data from other countries. This work was conducted using data recorded in the national Cattle Movement Monitoring System (CMMS). Culling refers to the exit of cows from the national herd, as a result of death but regardless of reason, and cow-culling rate was calculated as the number of cow exits (as defined above) each year divided by the number of calf births in the same year. Culling rate was determined by type (dairy or beef), date of birth, method of exit (slaughter or on-farm death), month of exit and interval between last calving and exit. The average cow-culling rate during 2003 to 2006 was 19.6% (21.3% for dairy, 18% for beef). While comparisons must be treated with caution, it concluded that the overall rates of culling in Ireland fell within published internationally accepted norms. The on-farm mortality rate of 3.2-4.1% was similar to that reported in comparable studies.  相似文献