An improved vitrification protocol for equine immature oocytes,resulting in a first live foal          下载免费PDF全文

N. Ortiz‐Escribano  O. Bogado Pascottini  H. Woelders  L. Vandenberghe  C. De Schauwer  J. Govaere  E. Van den Abbeel  T. Vullers  C. Ververs  K. Roels  M. Van De Velde  A. Van Soom  K. Smits 《Equine veterinary journal》2018,50(3):391-397

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Comparative analysis of ovarian transcriptomes between prolific and non‐prolific goat breeds via high‐throughput sequencing          下载免费PDF全文

X‐D Zi  J‐Y Lu  H Zhou  L Ma  W Xia  X‐R Xiong  D‐L Lan  X‐H Wu 《Reproduction in domestic animals》2018,53(2):344-351

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Equine foetal gender determination in mid‐ to late gestational mares: A practical inquiry          下载免费PDF全文

Margot Van de Velde  Kim Roels  Cyrillus Ververs  Ilse Gerits  Jan Govaere 《Reproduction in domestic animals》2018,53(5):1027-1032

In recent years, the interest in equine foetal gender determination (FGD) during gestation increased remarkably. Ultrasonographic FGD can be performed in two different periods during gestation. The earliest examination can take place at a gestational age of 60–70 days, whereby the genital tubercle is used to differentiate between male and female foeti. The time window of the second approach is wider (120–210 days), and there are more characteristics to take into consideration. In this article, the feasibility and accuracy of ultrasonographic FGD in mid‐ to late gestation are evaluated. One hundred twenty‐one mares from different breeds with a pregnancy stage between 120 and 270 days were examined once, using B‐scale ultrasonography (Esaote MyLab™ClassC). None of the mares were sedated nor shaved, and the procedure was completed within 15 min. Diagnosis was firstly based on the gonads. The final judgement was made based on all visible foetal reproductive organs. In three cases with a pregnancy stage beyond 257 days, FGD was not possible. All of the examined mares in which a FGD could be performed gave birth to a healthy foal. In 98% of the examinations (116/118), the diagnosis was correctly made. In both cases of misdiagnosis, only one characteristic was seen during the procedure and wrongly interpreted. Beyond 210 days of pregnancy, the extremities can preclude a good visualization of the inguinal region. In conclusion, equine FGD in mid‐ to late gestation is an accessible and accurate technique, although a good ultrasound device is a prerequisite and experience and expertise is necessary.  相似文献   

2018年山东省诸城市小反刍兽疫免疫效果横断面调查     

李焕明  郑召芹  谢海鹏  党安坤  徐淑华  兰邹然 《中国动物检疫》2018,35(10):25-27

为掌握山东省诸城市小反刍兽疫的免疫效果,2018年6月采用横断面研究方法,对该市规模场和自然村（散养户）饲养羊只进行小反刍兽疫免疫效果评估。采用两阶段随机抽样方法,结合部分便利抽样,采集580份羊血清样品进行ELISA 抗体检测。结果显示：合格样品560份,合格率达96.5%;14个规模场均免疫合格,群体合格率达到100%;在抽检的16个自然村中,15个村免疫合格。结果表明,诸城市小反刍兽疫总体免疫效果良好。  相似文献   

2017年全球低致病性禽流感疫情分析     

陈泽金  兰邹然 《中国动物检疫》2018,35(5):12-15

基于世界动物卫生组织（OIE）全球动物卫生信息系统的疫病报告数据统计,2017年全球共有10个国家或地区报告发生57起低致病性禽流感疫情,除大洋洲和南极洲外,其他4大洲均有疫情报告。报告发现的低致病性禽流感亚型包括H5N1、H5N2、H5N3、H5N6、H5N9、H7N3、H7N6和H7N9等多个亚型,其中H5N2亚型疫情数最多,报告20起。从时间分布上看,气温偏低的1—3月份报告的疫情数最多,共报告40起。从疫情发生场点类型看,绝大多数疫情发生在规模场。综合来看,2017年全球低致病性禽流感疫情呈散发态势,但流行亚型众多,应持续保持关注。  相似文献   

Effect of weaning age and the use of different sized Artemia nauplii as first feed for jade perch Scortum barcoo     

Stijn Van Hoestenberghe  Mathieu Wille  Evelien De Swaef  Bruno M. Goddeeris  Nancy Nevejan 《Aquaculture International》2015,23(6):1539-1552

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BPA不影响卵母细胞减数分裂相关基因Dazl的甲基化   总被引：1，自引：0，他引：1  

章寒琼  张西锋  潘博  沈伟  李兰 《青岛农业大学学报(自然科学版)》2011,(2):83-87

为了探讨环境雌激素BPA对小鼠卵母细胞减数分裂相关基因Dazl甲基化的影响,本研究通过给孕鼠饮用含有BPA的水方式使胎鼠在发育过程中接触BPA,利用重亚硫酸盐测序法,分析了胎鼠生殖嵴卵母细胞不同发育时期Dazl甲基化水平的变化。结果显示:Dazl在减数分裂期间处于低甲基化水平,无论对照组或处理组均低于10%,说明Dazl的低甲基化对维持减数分裂的正常进行有重要作用;对照组与处理组的甲基化水平相当,差异不显著,说明本研究的BPA浓度不影响卵母细胞Dazl的甲基化水平。  相似文献   

The Effect of Glycyl-Glutamine Dipeptide Concentration on Enzyme Activity,Cell Proliferation and Apoptosis of Jejunal Tissues from Weaned Piglets     

Wang Hui  Jia Gang  Chen Zheng-li  Huang Lan  Wu Cai-mei  Wang Kang-ning 《中国农业科学(英文版)》2011,(7):1088-1095

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碳纳米管的酸化处理对碳纳米管/聚氨酯复合材料微观结构及性能的影响     

张岚  顾继友  姜宪凯 《东北林业大学学报》2011,39(2):75-78

对多壁碳纳米管进行酸化处理,并采用原位聚合法制备了碳纳米管/聚氨酯复合材料。利用X射线光电子能谱分析(XPS)、电子扫描显微镜(SEM)、动态力学分析(DMA)研究了碳纳米管酸化与否对复合材料性能的影响。结果表明,碳纳米管经酸化处理后产生了羧基,碳纳米管的原位加入使得复合材料的储存模量和玻璃化转变温度都有所提高,而且经过酸化的碳纳米管对聚氨酯材料的改性要比未酸化碳纳米管对聚氨酯材料的改性效果更为显著。  相似文献   

13个广西喜树种源的喜树碱含量分析     

刘毅  苏付保  粟本超  冯立新  兰健花 《广西林业科学》2017,46(1)

以13个广西喜树(Camptotheca acuminata)种源的嫩叶为试材,研究不同种源对喜树碱含量的影响。结果表明,不同种源的喜树碱含量有极显著差异,不同土壤类型和海拔种源间的喜树碱含量有显著或极显著影响。13个广西喜树种源的喜树碱含量从高到低排序为德保田林金秀隆林德峨兴安西林天峨永福凌云东兰隆林新州南丹灵川。德保、田林、金秀喜树种源是优良种源。  相似文献