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21.
Gyandeep Gupta Munish Kumar Parimal Sardar Tincy Varghese Prem Prakash Srivastava Subodh Gupta 《Aquaculture Research》2021,52(1):260-272
An experiment was carried to determine the plasma fenbendazole (FBZ) concentration and physio‐metabolic responses in juveniles of Labeo rohita (90 ± 4 g) after oral administration of single doses at 10, 20 and 50 mg, 20 mg FBZ/kg b.wt. in multiple times on 1st, 3rd and 7th day. The blood samples were collected at 0.5, 1, 2, 4, 8, 12, 24, 30, 48, 72, 96 and 120 hr, after single‐dose administration, and regularly (upto 15 day) in multiple dose. Plasma FBZ concentration was determined up to the limit of detection (LoD) of 0.09 µg/ml by HPLC. There was no parent drug detected in plasma for administration of 10 mg FBZ/kg b.wt. The drug attained the peak concentration (Cmax) 1.85 and 3.09 µg/ml in plasma at 4 hr (Tmax) after administration of 20 and 50mg FBZ/kg b.wt. respectively. Plasma FBZ was detectable up to 96 and 120 hr with concentration 0.09 ± 0.007 and 0.098 ± 0.006 µg/ml, respectively, after single‐dose administration of 20 and 50mg/kg b.wt. In case of multiple‐dose administration, the maximum concentration of FBZ was 1.01 ± 0.03 µg/ml on 7th day that was less than to the single dose at 50 mg/kg b.wt. However, FBZ was detected up to 11 day after multiple doses. The study revealed that the hepatic antioxidant enzymes activities like superoxide dismutase, catalase and glutathione‐S‐transferase were significantly affected by increasing FBZ in single and multiple doses. The results of the present study could reveal that single‐ or multiple‐oral administration of FBZ at 20 mg/kg b.wt. in feed as antihelminthic drug in L. rohita could be considered as the safe dose. 相似文献
22.
Rapid screening of selected European winter wheat varieties and segregating populations for the Glu-D1d allele using PCR 总被引：2，自引：0，他引：2
Forty-two winter wheat varieties and 193 F2 and BC1F2 seeds were screened for Glu-D1d allele encoding the HMW glutenin subunits 5 + 10 using polymerase chain reaction (PCR). The segregating populations originated from crosses involving wheat parents with good and poor bread-making quality. A clear PCR product of 450 bp, representing 1Dx5 of the Glu-D1d allele was identified in 24 varieties and 111 hybrid seeds. Four different Glu-D1 alleles: a (2 + 12), b (3 + 12), c (4 + 12) and d (5 + 10) were detected using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Only genotypes possessing Glu-D1d gave a positive PCR signal, hexaploid triticale and 4 × wheat lacking Glu-D1 locus gave a negative signal. The efficiency of PCR selection for bread-making quality in early generations using half seed is discussed. 相似文献
23.
Jiby Mary Varghese Alangar Ishwara Bhat 《Journal of Crop Science and Biotechnology》2011,14(4):247-254
A protocol was developed for an efficient Agrobactertium-mediated transformation of black pepper plants through somatic embryogenesis. Embryogenic mass derived from primary somatic
embryos that were obtained from the micropylar region of mature germinating seeds of black pepper was found to be the ideal
target tissue for transformation. Genetic fidelity test of embryogenic mass-derived plantlets by RAPD using 23 random primers
revealed no genetic variation among the progenies and the parent plant. Among the antibiotics used for selection of transformants,
cefotaxime at 100 μg mL−1 was found to be optimum to control Agrobacterium besides its ability to promote somatic embryo proliferation. In the case of kanamycin, a step-wise increase in concentration
from 25 to 50 and then to 100 μg mL−1 were found to be optimum. Embryogenic mass co-cultivated with Agrobacterium carrying the β-glucuronidase (GUS) reporter gene were cultured on plant growth regulator-free Schenk and Hildebrandt (SH)
medium and transformants were selected in selection medium containing cefotaxime and step-wise increase in kanamycin concentration.
The transient GUS gene expression was determined histochemically. Transformants that survived in the selection medium were
hardened in the greenhouse. An average of nine hardened putative plantlets was obtained per gram of embryogenic mass. The
presence of transgene in these plantlets was assayed by PCR, dot blot, and Southern blot hybridization. Results presented
demonstrated for the first time an efficient transformation and regeneration of black pepper without the use of growth regulators.
This simple efficient procedure would allow transformation of black pepper with genes of desirable characters. 相似文献
24.
Prabu Dhanasekaran Linga Vijayagopal Pananghat Ebeneezar Sanal Kalidas Chellappa Rameshkumar Palsamy Varghese Eldho Muniswaran Bose Ramar 《Fish physiology and biochemistry》2022,48(3):785-804
Fish Physiology and Biochemistry - In a feeding experiment, cottonseed meal (CSM) was used to replace fishmeal (FM) in the diet of snubnose pompano, Trachinotus blochii, supplemented with lysine... 相似文献
25.
A field experiment was conducted to assess the effect of microbial inoculants and inorganic fertilizers for sustaining the yield of soybean. Application of 100% recommended dose of fertilizer (RDF) gave significantly highest yield (2433 kg ha?1) over 75% RDF (2317 kg ha?1) and without RDF (2205 kg ha?1). Seeds inoculated with Rhizobium (Bradyrhizobium japonicum) and phosphate-solubilizing bacteria (2480 kg ha?1) gave significantly highest soybean yield over without inoculation (2191 kg ha?1). Rhizobium and phosphate-solubilizing bacteria with 100% RDF (2674 kg ha?1) gave significantly highest seed yield than rest of the treatment combinations. Root nodules and their dry weight were remained un-influenced due to fertilizer levels, whereas in bio-fertilizers, it was significantly higher with Rhizobium inoculation (24.3 and 408 mg, respectively) followed by dual inoculation of Rhizobium and PSB. 100% RDF and dual inoculation with Rhizobium and PSB earned Rs. 47916/- and Rs. 51182/- net returns per ha, respectively. 相似文献
26.
The effect of feeding Spirulina platensis on the growth, carcass composition, organoleptic quality, digestive enzyme activity and digestibility of common carp, Cyprinus carpio L., was studied through a culture trial lasting 120 days. Four experimental diets were employed by replacing fish meal protein from the standard diet at 25%, 50%, 75% and 100% through the incorporation of Spirulina. Another diet with Spirulina as the sole source of protein was also used. The final weight gain, specific growth rate, food conversion ratio and protein efficiency ratio of common carp were not affected by Spirulina supplementation. However, the diet with Spirulina as the sole source of protein resulted in better net protein retention. The muscle RNA:DNA ratio of fish fed Spirulina diets was higher than that of fish fed control diet. There was no significant difference in carcass moisture and protein content in the fish fed Spirulina diets as compared to fish-meal-based control diet. The carcass ash and fat contents were positively and negatively correlated with dietary Spirulina level, respectively. Organoleptic evaluation revealed no effect of Spirulina feeding on the quality of both raw and cooked fish. The gut digestive enzyme activity did not show any definite trend with respect to Spirulina supplementation. Spirulina improved the protein digestibility of the diets. 相似文献
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Shabanamol S Edna Mary Varghese Meenu Thampi Karthika S Sreekumar J 《Communications in Soil Science and Plant Analysis》2020,51(9):1268-1282
ABSTRACT Nitrogen (N) is the “prime” among the three major nutrients required for plant growth. The large potential demand of nitrogen for plant growth is mainly achieved through biological nitrogen fixation. We have attempted to study the growth enhancement of rice crop under greenhouse conditions using the endophytic diazotrophic bacteria Lysinibacillus sphaericus. The effect of inoculation of L. sphaericus on yield and nutrient uptake was studied. All the parameters studied showed significantly higher values than that obtained in uninoculated absolute control. The highest yield was given by treatment T7 (seed and foliar) followed by T9 (soil and foliar) and T5 (seed and soil) and were statistically at par. The highest dry weight of grains was found to be in treatment T2 (13.0 g?1plant) which received soil treatment of L. sphaericus followed by T9 (12.2 g?1plant) which received combined application of L. sphaericus as soil and foliar treatment. It can be deduced from the results, there is a high influence of endophytic bacteria L. sphaericus in plant growth despite the mode of inoculation. This establishes the potential of diazotrophic endophyte L. sphaericus to limit the use of industrial N fertilizers thereby enhancing the fertility of the soil. 相似文献
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The applicability of random amplified polymorphic DNA (RAPD) markers in the cultivated rubber tree, Hevea, was evaluated using 43 decamer oligonucleotide primers in a set of 24 clones selected in different South-East Asian countries. A total of 220 0.35–3.5 kb DNA fragments were amplified, of which 111 were polymorphic. Of these, 80 fragments (RAPD markers) which were repeatable and clearly scorable across all genotypes were used to estimate genetic distances among the clones tested. The estimated genetic distances ranged from 0.05 (RRII 308 and PB 5/51) to 0.75 (RRIC 100 and SCATC 88–13). A mean genetic distance of 0.5 indicates a rather high genetic variability among the tested clones. As expected, because of the breeding history of Hevea, UPGMA cluster analysis and Principal Coordinate Analysis (PCoA) indicated the absence of a distinct geographical grouping. The possible application of RAPD markers for clone identification and also for analysis of genetic relationships among Hevea clones is discussed. 相似文献