检测砂梨潜隐病毒的IC-RT-PCR和TC-RT-PCR的研究   总被引：1，自引：0，他引：1  

邓晓云  洪霓  胡红菊  王国平 《果树学报》2004,21(6):569-572

以砂梨为试材,在生物学和血清学检测的基础上,采用免疫捕作RT-PCR穴IC-RT-PCR雪和试管捕作RT-PCR穴TC-RT-PCR雪技术,对苹果褪绿叶斑病毒(Applechloroticleafspotvirus,ACLSV)和苹果茎沟病毒(Applestemgroovingvirus,ASGV)进行了检测分析。结果表明,TC/IC-RT-PCR均能有效检测梨粗提液中的ACLSV和ASGV,分别获得了大小约358bp和499bp的目标扩增片段;但IC-RT-PCR检测ACLSV的效果受到病毒分离株间血清学关系影响。与传统的RT-PCR相比,IC/TC-RT-PCR不仅灵敏度更高,而且不需提取总RNA,可以简化操作程序和减少苯酚、氯仿类有机试剂对人体的伤害和对环境的污染,适合于大量梨样品的病毒快速灵敏检测。  相似文献   

限水灌溉对转基因抗虫棉产量形成的影响     

刘生荣  刘党培  易永华  贾涛 《干旱地区农业研究》2004,22(4):65-67,74

采取限水灌溉的方法,研究了灌水对棉花产量形成的影响。研究结果表明,合理灌水能促进棉花开花成铃,延长最佳成铃时段,增加棉株优质节位的成铃数,单株籽棉干物重增加23.4%～61.9%,产量提高16.8%～45.3%。灌水量以675m3/hm2效果最好,灌水效益高达0.57kg/m3,灌水量达1350m3/hm2增产幅度下降,灌水效益仅0.21kg/m3。  相似文献   

塔里木河干流中游灌区退耕封育效益分析     

钟扬明  王红坤 《干旱区研究》2004,21(1):81-84

塔里木河干流中游灌区退耕封育的规模为 9× 10 3 hm2 ,其中 ,库灌区退耕 1.4 2× 10 3 hm2 ,河灌区退耕 3.5 7× 10 3 hm2 ,泵灌区退耕 4 .0 1× 10 3 hm2 。退耕封育后 ,农田灌溉用水改为生态灌溉用水 ,用水量减少。人工种植的农作物被天然野生植被所替代 ,同时可提高对保留耕地的利用率 ,扩大耕地的播种面积 ,增加地表植被的覆盖度。退耕封育后 ,可节约水量 0 .97× 10 8m3 ,除满足封育生态用水外 ,还可增加塔里木河下泄水量 0 .31× 10 8m3 。  相似文献   

利用人工增雨为农田需水服务的信息系统框架   总被引：1，自引：0，他引：1  

李应林  高素华 《干旱区研究》2004,21(3):246-249

针对我国北方地区水资源严重短缺、农田水分供需矛盾日益尖锐的现状 ,提出了首选人工增雨为农田补水的思路 ,并初步设计了一个利用人工增雨为农田需水服务的农田需水信息系统框架。  相似文献   

毛乌素沙地典型地形断面土壤水分动态     

杨佩国  李保国  吕贻忠 《干旱区研究》2004,21(4):333-337

基于毛乌素沙地的野外观测资料,对毛乌素沙地典型地形断面土壤水分动态进行分析。结果表明,土壤水分季节变化可划分为土壤水积聚期、消耗期和稳定期;根据土壤水分垂直变化可把土壤剖面划分为土壤水分易变层、利用层和调节层;沿着坡度减小的方向,各地形断面对应层次的土壤水分含量逐渐升高,丘间地土壤水分含量明显高于该断面上其余各点。  相似文献   

西狭文化旅游形象三重结构整合   总被引：2，自引：0，他引：2  

李文兵 《干旱区资源与环境》2004,18(3):116-120

西狭文化旅游资源是甘肃成县旅游开发的优势资源。由于对西狭文化理解上的不全面 ,导致西狭文化旅游区的建设存在一系列偏差 ,旅游形象不突出。文章提出了西狭文化旅游形象的三重结构整合的思路 ,即文化结构整合、空间结构整合与景观结构整合 ;并且认为 :文化结构整合是西狭文化旅游形象三重结构整合的前提、基础 ;空间结构整合是由文化结构整合向景观结构整合的过渡 ,而景观结构整合是西狭文化旅游形象三重结构整合的关键。  相似文献   

210国道榆林至陕蒙交界段建设的生态环境影响评价   总被引：3，自引：0，他引：3  

李团胜  刘引鸽 《干旱区资源与环境》2004,18(5):64-68

在简要分析榆林至陕蒙交界段生态环境对公路及公路建设的影响的基础上 ,重点论述了公路建设在建设期和营运期对当地生态环境的影响 ,并对公路建设前后水土流失与土地沙化状况进行了预测 ,最后提出水土保持与防沙护路的方案。结论是 :只要把公路建设与生态环境建设相结合 ,重视生态环境的保护与建设 ,该公路建设和营运对生态环境的不利影响是很小的 ,而且 ,由于公路建设本身的需要所采取的防沙护路措施 ,会使公路沿线的生态环境更好  相似文献   

山杏幼苗水分生理生态特性及凋萎湿度的研究   总被引：7，自引：0，他引：7  

李雪华  蒋德明  刘志民 《干旱区资源与环境》2004,18(5):168-171

对山杏幼苗进行 3种施水量的水分培养和干旱处理。结果表明 :随施水量的减少 ,叶片的净光合速率、蒸腾速率、气孔导度和水分利用效率等指标都随之下降 ,叶片温度提高。 40 9.2和 1 89.2 mm施水量对山杏幼苗各项指标的影响呈显著差异 ,而 40 9.2和 32 1 .2 mm之间与 32 1 .2和 1 89.2 mm之间均无差异。随土壤干旱的加剧 ,山杏幼苗叶片的蒸腾速率和水势与土壤含水量呈规律性变化 ;当干旱持续 39天时 ,1 .44%的土壤含水量可视为山杏幼苗的凋萎湿度  相似文献   

Model establishment and biological behaviour observation of mouse blastocyst co-cultured with hepatocarcinoma cell lines with differently invasive and metastatic potential in vitro     

LI Da-qiang  WANG Zhi-biao  BAI Jin  ZHAO Jie  HU Kai  WANG Yuan  DU Yong-hong 《园艺学报》2004,20(1):32-37

AIM: To explore interaction and biological behaviour changes of two kinds of cells-blastocysts and hepatocarcinoma cells in the same microenvironment. METHODS:The models of mouse blastocysts co-cultured with human hepatocarcinoma cell lines were established, then biological behaviours and mutual effects of the two kinds of cells in co-culture system were observed. RESULTS: Compared with control group, hepatocarcinoma cells with differently invasive and metastatic potential significantly enhanced the rates of blastocyst hatchment , attachment and outgrowth(P＜0.05). There was no significant difference in those among hepatocarcinoma cells co-cultured groups (P＞0.05). The blastocyst hatched and attached to hepatocarcinoma cells with differently invasive and metastatic potential. Then, differential trophoblasts invaded hepatocarcinoma cells. The clear-cut interfaces were gradually formed between both sides. Hepatocarcinoma cells on interface showed changes of growth direction and cell shapes and did not invade blastocysts. CONCLUSIONS: Hepatocarcinoma cells promoted blastocyst development. Blastocysts implanted and invaded hepatocarcinoma cells with differently invasive and metastatic potential in vitro, which indicate that blastocyst implantation in vitro does not relate with the kinds and differential level of interactional cells and the low selectivity maybe relate with high adaptability of early life.  相似文献   

Rat mesenchymal stem cells differentiate into neuron-like cells induced by berberine in vitro     

XIANG Ping  LI Hai-biao 《园艺学报》2004,20(1):51-53

AIM: To investigate whether berberine can induce rat mesenchymal stem cells (MSCs) to differentiate into neuron -like cells in vitro. METHODS: MSCs were separated from young rat femurs marrow and expanded in culture medium. MSCs were induced to differentiate by berberine. The morphological changes of MSCs were evaluated by light microscope.Neuron-spcific enolase (NSE), neurofilament (NF), glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. RESULTS: Induced by berberine for 8 hours,MSCs exhibited neurotype . The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express. CONCLUSION: Berberine may induce adult rat MSCs to differentiate into neuron-like cells in vitro.  相似文献