Relationship between uPA and NF-κB p65 expression and its clin ical significance in breast cancer     

WANG Hong  WU Jin-min  CAO Hou-jun  ZHOU Wen-peng  FANG Yong  ZHANG Xing  ZHENG Shu 《园艺学报》2006,22(6):1218-1221

AIM:To evaluate the relationship between u PA and NF-κB p65 expression and its clinical significance in breast cancer.METHODS:The uPA mRNA was measured by real-time fluorescent quan t itative PCR in 46 cases of breast cancer tissues and their adjacent counterparts .NF-κB p65 were measured using immunohistochemistry.RESULTS:The expression of uPA gene was elevated in 63% of cases ,and there was a strong correlation between NF-κB p65 and uPA expression (r ＝0.451，P<0.01).In addition,the expression of uPA was associated with num bers of axillary lymph node involvement and tumor size.However,no significance was found in uPA expression in the age,pathological type and distant metastasi s organ classification.CONCLUSION:The uPA gene is highly expressed in most of the brea st cancer,and there is strong correlation to NF-κB p65 expression,number of l ymph nodes involvement and tumor size.  相似文献   

Expression of nerve growth inhibiting factor Nogo-A mRNA and protein in the brain ischemic infarction rats     

WU Gong-xiong  WANG Yu-ping  ZHANG Hai-wei△  ZHANG Chun-xiang  YAO Zhi-bin  XU Jie  LUO Xiao-qing  MA Si-qi 《园艺学报》2006,22(5):972-976

AIM: To investigate the changes of expression of Nogo-A at different time points in brain ischemic infarct rats. METHODS: The model of 80 cases of middle cerebral artery occlusion (MCAO) in rats was established. The expression of Nogo-A mRNA and protein were determined by Western blotting and hybridization, and the relationship between functional scoring and Nogo-A was also evaluated. RESULTS: In the brain of MCAO rats, Nogo-A mRNA expression was decreased on day 3 and increased significantly on day 7. The highest level was observed at the 21th d, keeping the same level at the 28th d. Nogo-A protein expression showed the same results. These results were correlated with the brain function scoring. CONCLUSION: Expression of Nogo-A does not increase in the early stage, but increases significantly in the late stage of MCAO, suggesting that Nogo-A expression may play an important role in the nerve regeneration of brain ischemic injury.  相似文献   

Hepatocyte growth factor protects hepatocytes from apoptosis induced by actinomycin D     

LI Wen-jun  LI Xiao-kun  CAI Shao-hui  QI Ren-bin  SU Zhi-jian  XIAO Jian  WU Zhi-ling 《园艺学报》2006,22(5):992-996

AIM: To investigate the anti-apoptotic effect of hepatocyte growth factor (HGF) on actinomycin D (ActD)-induced apoptosis in hepatocytes and the possible pathway. METHODS: Hepatocytes were exposed to ActD and HGF. The cytotoxic effects of ActD were tested by MTT. Apoptotic cells were identified by Hoechst 33342 staining, flow cytometry and detection of DNA fragmentation with agarose gel. Akt and phospho-Akt were detected by Western blotting analysis. RESULTS: The results showed that ActD induced apoptosis in hepatocytes 5 h after treatment. Phosphatidylinositol-3 kinase (PI-3K) specific inhibitor wortmannin enhanced the apoptotic effect of ActD. Furthermore, HGF significantly reduced the apoptosis in hepatocytes induced by ActD in a concentration-dependent manner. In the presence of wortmannin, HGF did not overcome apoptosis. CONCLUSION: Wortmannin enhances the apoptotic effect of ActD. HGF protects hepatocytes from apoptosis induced by ActD through a PI3K/Akt pathway.  相似文献   

Proliferative effect of neonatal mouse brain extracts on neural stem cells in vitro     

LIU Wei  XIAO Mei-ling  WU Jia-hua  LUO Huan-min 《园艺学报》2006,22(3):524-527

AIM: To discover specific neural stem cell (NSC) proliferation-promoting factor, which will contribute to study on development of nervous system and treatment of nervous system diseases. METHODS: The extracts of forebrain, midbrain,afterbrain and cerebellum of neonatal mice were prepared, and the NSCs of newborn mice were cultured in vitro. Neurospheres were observed, immunocytochemical staining of characteristic protein, nestin, and MTT assay were performed to identify NSCs and their proliferative properties. RESULTS: A great deal of neurospheres were formed in the presence of the extracts of afterbrain and cerebellum, which were positive for characteristic protein （Nestin） of NSC showed by immunocytochemical staining. CONCLUSION: The afterbrain and cerebellum extracts can increase the total number of NSCs isolated from newborn mice in vitro in a dose-dependent manner.  相似文献   

Peripheral blood mononuclear cells activated by graded zirconia-hydroxyapatite composite in vitro     

QUAN Ren-fu  YANG Di-sheng  MIAO Xu-dong  LI Wei  WU Xiao-chun  WANG Hong-bin 《园艺学报》2006,22(1):160-163

AIM: To evaluate the immunogenicity of a novel orthopedics materials (graded zirconia-hydroxyapatite composite) in vitro by using peripheral blood mononuclear cells (PBMCs) from healthy young people, and simple zirconia-hydroxyapatite composited material was used as control materials. METHODS: Proliferation of PBMCs cultured in different liquid after 5 days was measured by MTT methods. ELISA was used to detect TNF-α and IL-6 concentration in the supernatant of PBMCs cultured in the extracts after 24 hours. Flow cytometery was used to measure CD69 and CD25 in activated PBMCs cultured in the extracts of the two kinds of materials after 24 hours. RESULTS: The proliferation rate of the simple composite group was significantly lower than that in negative group (P<0.05), but there was no difference between the graded composited group and negative group. After 24 hours culture with LPS, the concentrations of TNF-α and IL-6 in the simple composited group were significantly higher than that in graded composited group, respectively (P<0.05). Cultured with PHA for 24 hours, the ratio of CD69 and CD25 positive PBMCs in the simple composited group was all significantly higher than that in graded composited material group (P<0.01). CONCLUSION: The number of PBMCs activated by the graded composited material is less than the simple composited material and the immunogenicity of the graded composited material is lower than the simple composited material.  相似文献   

Biological characters of mesenchymal stem cells of human umbilical cord blood     

ZHOU Dun-hua  WEI Jing  HUANG Shao-liang  FANG Jian-pei  BAO Rong  WU Yan-feng  LI Yang 《园艺学报》2006,22(8):1606-1609

AIM：To study the isolation,expansion and purification of mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB),and investigate some biological identities of MSCs.METHODS：(1) MSCs of UCB,adult bone marrow (BM) and fetus BM were isolated by centrifugation with Ficoll,and the different kinds of MSCs were observed everyday.(2) Surface markers of MSCs were identified by flow cytometry.(3) The level of HGFs (TPO,SCF,FLT-3L,IL-6) secreted by different sources of MSCs was checked by ELISA method.RESULTS：(1) No difference in morphology of the colonies between UCB MSCs and BM MSCs was observed.However,the mononuclear cells needed in culture of UCB MSCs was about 3 times more than that in culture of BM MSCs.The times of UCB MSCs colony formation and confluencing were longer than that of BM in primary culture.(2) After passaged,there was no significant difference in the proliferation rates of 3 kinds of MSCs.Only 4 of 15 UCB samples contained a homogeneous population of MSCs.(3) UCB MSCs shared the same markers with BM MSCs.Neither hematopoietic marker nor immunologic recognition antigens were expressed.(4) The level of hematopoietic growth factors (HGFs) secreted by 3 kinds of MSCs was similar.CONCLUSIONS：(1) MSCs were isolated from UCB,but the amount of MSCs in UCB was smaller than that in BM,and just seldom samples of UCB contained homogeneous MSCs.(2) MSCs from UCB and BM shared the same biological characteristics,such as proliferation ability,surface markers,immunophenotypes and HGFs secretion.  相似文献   

嘧肽霉素抗烟草花叶病毒作用机理初步研究   总被引：12，自引：0，他引：12  

朱春玉  吴元华  赵秀香  王艳红 《植物病理学报》2006,36(4):314-316

 以烟草花叶病毒(TMV)/烟草为测试体系,用³H-尿苷和³H-亮氨酸2种同位素示踪的方法研究了4%嘧肽霉素水剂抗TMV的作用机理。通过³H-尿苷示踪实验发现:无论是叶盘法还是整株法测定,嘧肽霉素均能抑制病毒对³H-尿苷的利用,抑制率分别为72.43%和68.95%;而对寄主植物RNA合成,2种方法的抑制率分别为8.09%和11.02%。通过³H-亮氨酸示踪结果可以看出:嘧肽霉素较强地抑制了病毒对³H-亮氨酸的吸收,但能促进寄主对³H-亮氨酸的吸收。³H-尿苷和³H-亮氨酸分别是RNA和蛋白质合成的前体物,因此,嘧肽霉素通过抑制病毒RNA复制和蛋白质的合成来扰乱病毒的增殖,从而减弱病害的发生。  相似文献   

甘薯地土壤线虫群体分布规律及取样方法研究   总被引：2，自引：0，他引：2  

海棠  武保悦  金凤柱  胡跃高 《中国植保导刊》2006,26(10):7-10

在5年连作甘薯地,于甘薯移栽前和移栽后的不同时间,调查甘薯穴中和穴间、株间和行间以及各样点不同土层线虫的群体数量。分析明确了植物寄生线虫和非植物寄生线虫群体的水平分布和垂直分布规律,提出了研究和了解甘薯地土壤线虫发生动态的调查取样方法。  相似文献   

昆虫的行为节律   总被引：7，自引：1，他引：7  

吴少会  向群  薛芳森 《江西植保》2006,29(4):147-157

本文主要阐述了昆虫中几种常见的行为节律和一些相关研究的进展情况,论述了各行为节律的作用机制及影响因素,有助于掌握昆虫种群的活动规律,为害虫防治提供重要依据。  相似文献   

云南主要烟区烟蚜种群解毒酶活力比较   总被引：2，自引：0，他引：2  

宋春满邓建华  吴兴富雷朝亮 《昆虫天敌》2006,28(2):55-61

通过测定烟蚜Myzus persicae(Sulzer)的α-NA羧酸酯酶、β-NA羧酸酯酶、谷胱甘肽转移酶和乙酰胆碱酯酶的活力,比较了云南5个主要烟区田间烟蚜种群的4种酶的活力频率分布,结果表明,云南5个烟区的烟蚜田间种群的α-NA羧酸酯酶、β-NA羧酸酯酶和乙酰胆碱酯酶的高活力个体均以楚雄和昆明种群较高,昭通种群和丽江种群较低。谷胱甘肽转移酶在5个烟区烟蚜种群间差异不大。  相似文献