The Safety Evaluation of Cefalonium Intramammary Infusion (Dry Cow)     

HUA WeiYi  LIU YiMing  XU Fei  LU YongQiang  KONG Mei  WANG HaiTing  HUANG HuiLi  WANG HongLei  WU LianYong  LI XiuBo 《中国农业科学》2019,52(2):359-366

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Screening and Verification of Low Nitrogen Tolerant and Nitrogen Sensitive Cotton Germplasm     

Wang Zhun  Zhang Hengheng  Dong Qiang  Gui Huiping  Wang Xiangru  Pang Nianchang  Li Yongnian  Niu Jing  Jin Dingsha  Wang Sujie  Zhang Xiling  Song Meizhen 《棉花学报》2021,32(6):538-551

[Objective] The aim of this study is to evaluate nitrogen efficient cotton germplasms and improve nitrogen use efficiency. [Method] Eighty cotton germplasms were selected and evaluated in the hydroponic experiment under low (0.25 mmol·L^－1) and high (5 mmol·L^－1) nitrogen concentration. Different traits for screening were identified and nitrogen use efficiency types were classified. Field experiments were also performed for comparison and confirmation of the identified germplasms. [Result] The results showed that there were significant differences in the total plant dry matter, shoot nitrogen accumulation and nitrogen absorption efficiency in cotton germplasms at the two nitrogen levels. Based on coefficient of variation, principal component analysis and correlation, six traits including total plant dry matter, shoot dry matter, root dry matter, total nitrogen accumulation, shoot nitrogen accumulation and nitrogen absorption efficiency were used as screening indicators. According to the Heatmap clustering analysis and the nitrogen efficiency comprehensive index, two germplasms (Lu05R59 and CCRI 69) were identified as low nitrogen tolerant and nitrogen efficient, and two germplasms (Coker 201 and Xinluzhong 30) as low nitrogen sensitive and nitrogen inefficient. The results of field experiment were consistent with the results of the hydroponic culture at the seedling stage. [Conclusion] It was finally determined that Lu05R59 and CCRI 69 were the low nitrogen tolerant and nitrogen efficient germplasms, and Coker 201 and Xinluzhong 30 were low nitrogen sensitive and nitrogen inefficient germplasms. The results of these studies provide the possibility for screening and rapid identification of nitrogen use efficiency in cotton at the seedling stage, and provide the ideal materials and theoretical basis for further study of cotton nitrogen efficient.  相似文献   

Regulatory role of l-proline in fetal pig growth and intestinal epithelial cell proliferation     

Jing Wang  Bi'e Tan  Jianjun Li  Xiangfeng Kong  Minjie Tan  Guoyao Wu 《动物营养（英文）》2020,6(4):438

l-proline (Pro) is a precursor of ornithine, which is converted into polyamines via ornithine decarboxylase (ODC). Polyamines plays a key role in the proliferation of intestinal epithelial cells. The study investigated the effect of Pro on polyamine metabolism and cell proliferation on porcine enterocytes in vivo and in vitro. Twenty-four Huanjiang mini-pigs were randomly assigned into 1 of 3 groups and fed a basal diet that contained 0.77% alanine (Ala, iso-nitrogenous control), 1% Pro or 1% Pro + 0.0167% α-difluoromethylornithine (DFMO) from d 15 to 70 of gestation. The fetal body weight and number of fetuses per litter were determined, and the small and large intestines were obtained on d 70 ± 1.78 of gestation. The in vitro study was performed in intestinal porcine epithelial (IPEC-J2) cells cultured in Dulbecco''s modified Eagle medium-high glucose (DMEM-H) containing 0 μmol/L Pro, 400 μmol/L Pro, or 400 μmol/L Pro + 10 mmol/L DFMO for 4 d. The results showed that maternal dietary supplementation with 1% Pro increased fetal weight; the protein and DNA concentrations of the fetal small intestine; and mRNA levels for potassium voltage-gated channel, shaker-related subfamily, member 1 (Kv1.1) in the fetal small and large intestines (P < 0.05). Supplementing Pro to either gilts or IPEC-J2 cells increased ODC protein abundances and polyamine concentrations in the fetal intestines and IPEC-J2 cells (P < 0.05). In comparison with the Pro group, the combined administration of Pro and DFMO reduced the expression of ODC protein and spermine concentration in the fetal intestine, as well as the concentrations of putrescine, spermidine and spermine in IPEC-J2 cells (P < 0.05). Meanwhile, the percentage of cells in the S-phase and the mRNA levels of proto-oncogenes c-fos and c-myc were increased in response to Pro supplementation, whereas depletion of cellular polyamines with DFMO increased tumor protein p53 (p53) mRNA levels (P < 0.05). Taken together, dietary supplementation with Pro improved fetal pig growth and intestinal epithelial cell proliferation via enhancing polyamine synthesis.  相似文献   

不同品种和密度对玉米产量的影响     

郭飞  吴正虎  王秀珍 《耕作与栽培》2020,(3):10-12,16

本文分析了新疆塔城市3个玉米品种在不同种植密度下的农艺性状和产量差异。结果表明,常规品种先玉335、郑单958在栽培密度为5 000株/667m~2的条件下有较好的表现,但密度进一步增加,倒折风险很大;和育187在5000株/667m~2的栽培密度条件下产量最高,适宜的高密度有进一步提升产量的潜力。  相似文献   

河北省杂粮生产现状及生产布局展望     

刘猛  刘斐  赵宇  夏雪岩  李顺国 《农学学报》2021,11(3):93-100

河北省是杂粮生产大省,且种类繁多。为稳定河北省杂粮生产发展,进一步调整河北省种植业结构提供参考依据,本文总结了河北省杂粮的历史生产现状,包括面积、单产、总产以及河北省各地市杂粮的分布,试图探索杂粮的生产布局与趋势,同时针对河北省杂粮历史单产水平变化趋势,分析了造成单产变化的成因,并简单提出建议。最后提出河北省杂粮生产布局的展望,将河北省杂粮生产区域布局划分为优势主产区、战略提升区、稳步发展区三个功能区。  相似文献   

工业级棉短绒清理回收工艺的改进     

王如南  张军  霍永峰  袁新英  韩文杰 《中国棉花》2021,48(8):26-28

为降低棉短绒中的杂质含量，提高棉短绒的质量及收率，对棉短绒清理回收的生产工艺进行了改进，创新连续化多级除杂装备和短绒精细化分离工艺。棉籽清理除杂工艺中，增加了圆筛、棒条机，并且调整了风力清籽机组配置；短绒分离工艺中，增加了五联筛和新型棒条机。改进后的工艺能够提升对毛棉籽的除杂效果，彻底清理剥绒得到的棉短绒中的碎棉仁、棉壳及棉秆等杂质。此外，该工艺还能回收棉短绒下脚料，满足大型生产线的生产要求。  相似文献   

EPS364, a Novel Deep-Sea Bacterial Exopolysaccharide,Inhibits Liver Cancer Cell Growth and Adhesion     

Yun Wang  Ge Liu  Rui Liu  Maosheng Wei  Jinxiang Zhang  Chaomin Sun 《Marine drugs》2021,19(3)

The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy.  相似文献   

十里香茶离体培养及再生体系研究     

杨霏  邹瑞  张玥  杨自云  吴田 《种子》2020,(4):46-49,54

为了保护珍贵的十里香茶树资源,掌握利于十里香茶的最优组培条件,本研究对十里香茶组培过程中外植体类型、消毒时间、培养基添加物及培养条件进行了筛选。结果表明,采用轻微木质化的带腋芽茎段经升汞消毒15 min,接种后暗培养5 d,MS+0.2 mg·L^-1 NAA培养基中添加3 mg·L^-1 PVP+2000 mg·L^-1 Vc或者6 mg·L^-1 PVP+3000 mg·L^-1 AC,可在低污染率的前提下将褐化率降为10%左右,一定程度上解决了茶树组培中褐化率高的难题;采用1/8 MS+1 mg·L^-1 IBA培养基有利于外植体生根且增殖迅速。本研究建立了十里香茶的无菌苗离体培养及再生体系,可通过组培手段对其进行大量繁殖。  相似文献   

高产、抗病转基因抗虫棉品种——中棉所94A915     

范术丽  王龙  庞朝友  魏恒玲  王寒涛  王晖  喻树迅 《中国棉花》2017,44(1):35-35

概述了中棉所94A915的选育经过、特征特性，并总结了其关键栽培技术。  相似文献   

体细胞克隆荷斯坦奶牛出生后死亡犊牛内脏器官的组织病理学观察     

孙伟  包向男  乌云高娃  王建国  李永胜  李善铎  张铁柱  王飞飞  李喜和 《中国农学通报》2020,36(20):114-118

为探究体细胞克隆荷斯坦奶牛出生后死亡的原因,对新生后死亡的克隆荷斯坦公牛和自然繁殖的荷斯坦公犊的主要组织器官进行比较。通过解剖和石蜡切片-HE(hematoxylin-eosin staining)染色技术,对主要的组织器官结构进行观察和分析。结果表明,新生后死亡的克隆荷斯坦公牛肺部结构清晰;肝脏的肝细胞明显肿大,出现脂肪轻度变性;肾小管上皮细胞出现变性;心肌的肌纤维间空隙增大;骨骼肌纤维间隙明显,空泡变性,这可能导致该克隆牛犊肌肉无力并功能不全;淋巴结皮髓质界限不分明,淋巴小结细胞较稀疏,生发中心不明显,淋巴窦细胞较少;脾脏内红细胞较少,这说明该克隆牛的造血功能可能不完善;胸腺的皮质部分和髓质部分界限不明显,嗜酸性胸腺小体不易辨认,可能发育不全。该克隆公牛免疫器官出现不同程度的发育不全现象较严重,这有可能是其出生后死亡率高的主要原因。  相似文献