Naturally occurring Eperythrozoon ovis infection in sheep reduces wool production     

MJ KABAY  FM SUNDERMAN  RB RICHARDS  TM ELLIS 《Australian veterinary journal》1992,69(9):232-232

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Assessment of Sperm Apoptosis in Cryopreserved Bull Semen After Swim-up Treatment: A Flow Cytometric Study     

A Chaveiro  P. Santos  FM da Silva 《Reproduction in domestic animals》2007,42(1):17-21

The techniques used to prepare bovine spermatozoa for in vitro fertilization, to enhance the percentage of motile sperm cells include the swim-up (SU) method, among others. The objective of the present study was to evaluate the phosphatidylserine (PS) translocation and plasma membrane integrity as the indicator of apoptosis and necrosis in post-thaw bull sperm after SU treatment using annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) assay. A flow cytometric method was employed to measure apoptosis levels on frozen-thawed bull spermatozoa. The assay detects PS translocation across the plasma membrane using a fluorescein-labelled annexin-V and PI. By using the annexin V/PI assay four different subpopulations of sperm were observed: (i) a population of apoptotic sperm, labelled with annexin V-FITC but not with PI; (ii) a population of early necrotic spermatozoa, sperm labelled with annexin-FITC and PI; (iii) a population of necrotic sperm, labelled with PI but not with annexin-FITC; and (iv) a population of fully viable sperm cells, sperm not labelled with annexin V-FITC and without PI. Results clearly indicated that SU technique itself could have an adverse effect on the spermatozoa membrane stability. It has also been found, significant differences between bulls in the levels of apoptotic sperm, after SU treatment.  相似文献   

用牦牛睾丸细胞生产羊传染性脓疱皮炎疫苗效果观察     

孔繁梅  史全军  宗晓龙  姜晓红  李毅恒 《中国兽药杂志》2006,40(4):58-58,59

为满足生产批量化要求，用牦牛睾丸细胞代替犊牛睾丸细胞，接种G0-BT弱毒株，收获，加适当保护剂，经冷冻真空干燥制备羊传染性脓疱皮炎疫苗。结果半成品及成品检验均符合《规程》标准，取得了良好的效果。  相似文献   

THE ISOLATION OF MYCOPLASMAS FROM PORCINE PNEUMONIAS     

Judy  Marley  B.Sc.  P. B. Spradbrow  B.V.Sc.  Ph.D. D. A. Watt  B.V.Sc. 《Australian veterinary journal》1971,47(8):375-378

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The recovery of immunoglobulin G from horses by combination of selective plasmapheresis and forced flow electrophoresis     

E F Logan  A Stenhouse  J G Watt  A E Clark 《Research in veterinary science》1970,11(5):431-437

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PIONEERS AND THEIR PROBLEMS     

O. M. Watt 《Veterinary anaesthesia and analgesia》1974,5(1):50-54

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Tibial plateau levelling     

Watt P 《Australian veterinary journal》2000,78(6):385-386

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Sedimentary 12-n-Propylcholestanes, Molecular Fossils Diagnostic of Marine Algae     

Moldowan JM  Fago FJ  Lee CY  Jacobson SR  Watt DS  Slougui NE  Jeganathan A  Young DC 《Science (New York, N.Y.)》1990,247(4940):309-312

Certain C(30)-steranes have been used for identifying sedimentary rocks and crude oils derived from organic matter deposited in marine environments. Analysis of a C(30)-sterane from Prudhoe Bay oil indicates that these C(30)-steranes are 24-n-propylcholestanes that apparently are derived from precursor sterols 24-n-propylidene-cholesterols and 24-n-propylcholesterol. These widely occurring sterols are biochemically synthesized in modern oceans by members of an order (Sarcinochrysidales) of chrysophyte algae. These data thus imply that C(30)-sterane biomarkers in sedimentary rocks and crude oils have a marine origin. Screening of a few organic-rich sedimentary rocks and oils from throughout the Phanerozoic suggests that these C(30)-steranes first appeared and, therefore, their source algae evolved between Early Ordovician and Devonian.  相似文献   

Neural activity triggers neuronal oxidative metabolism followed by astrocytic glycolysis     

Kasischke KA  Vishwasrao HD  Fisher PJ  Zipfel WR  Webb WW 《Science (New York, N.Y.)》2004,305(5680):99-103

We have found that two-photon fluorescence imaging of nicotinamide adenine dinucleotide (NADH) provides the sensitivity and spatial three-dimensional resolution to resolve metabolic signatures in processes of astrocytes and neurons deep in highly scattering brain tissue slices. This functional imaging reveals spatiotemporal partitioning of glycolytic and oxidative metabolism between astrocytes and neurons during focal neural activity that establishes a unifying hypothesis for neurometabolic coupling in which early oxidative metabolism in neurons is eventually sustained by late activation of the astrocyte-neuron lactate shuttle. Our model integrates existing views of brain energy metabolism and is in accord with known macroscopic physiological changes in vivo.  相似文献   

Microinjected c-myc as a competence factor   总被引：54，自引：0，他引：54  

L Kaczmarek  J K Hyland  R Watt  M Rosenberg  R Baserga 《Science (New York, N.Y.)》1985,228(4705):1313-1315

While a number of oncogenes are expressed in a cell cycle-dependent manner, their role in the control of cell proliferation can only be established by a direct functional assay. The c-myc protein, upon microinjection into nuclei of quiescent Swiss 3T3 cells, cooperated with platelet-poor plasma in the stimulation of cellular DNA synthesis. This suggests that c-myc protein, like platelet-derived growth factor (PDGF), may act as a competence factor in the cell cycle to promote the progression of cells to S phase. The presence in the medium of an antibody against PDGF abolished DNA synthesis induced by microinjected PDGF; however, the microinjected c-myc protein stimulated DNA synthesis even when its own antibody was present in the medium. The c-myc protein may act as an intracellular competence factor, while PDGF expresses its biological activity only from outside the cells.  相似文献