Effects of estrone infusion on secretion of F series prostaglandins and on function of the corpus luteum during late gestation in cattle     

C A Pimentel  R Vijayakumar  P G Weston  S M Pimentel  W C Wagner  J E Hixon 《American journal of veterinary research》1986,47(9):1972-1977

Catheters were surgically implanted in the carotid artery, jugular vein, and middle uterine vein of 8 cows at 245 days of gestation. Four cows were given 4 mg of estrone/hour via continuous jugular infusion from 0800 hours on day 246 of gestation through 0800 hours on day 250 of gestation; the remaining 4 cows (controls) were given the vehicle for estrone at 10 ml/hour for the same period. Blood samples were collected from the carotid artery every 3 hours during the infusion. Samples were collected hourly from the middle uterine vein from 0 through 8, 54 through 66, and 112 through 120 hours of the infusion periods. After completion of the infusion, corpora lutea were enucleated and blood samples were collected from the carotid artery and uterine vein at hourly intervals for an additional 8 hours. Dispersed cell preparations of the corpora lutea were incubated with and without luteinizing hormone (LH) or dibutyryl cyclic adenosine monophosphate (dbcAMP). Circulating concentrations of unconjugated and conjugated estrone and estradiol-17 beta were higher (P less than 0.05) in the group infused with estrone than in the vehicle-infused group. Mean and base-line concentrations of F series prostaglandins (PGF) for each blood collection period tended to increase (P less than 0.10) during infusion with estrone, but not during infusion with vehicle. After luteectomy, mean and base-line concentrations of PGF also tended (P less than 0.10) to be greater in the estrone-infused cows than in the control cows, but a surge in PGF concentrations due to removal of the ovarian source of progesterone did not develop.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Association of parainfluenza-3 virus with bovine macrophages and blood cells: an in vitro study   总被引：1，自引：0，他引：1  

E H Stauber  K J Weston 《American journal of veterinary research》1984,45(3):583-585

Bovine blood cells and peritoneal and lung macrophages were exposed in vitro to parainfluenza-3 (PI-3) virus. Residual nonadsorbed PI-3 virus (expressed in percentage of input virus) in the supernate of the various cell fractions 1 hour after incubation at 37 C was as follows: lung macrophages, 11%; peritoneal macrophages, 59%; monocytes, 26%; RBC, 14%; lymphocytes, 28%; and polymorphonuclear cells (PMN), 63%. Lung macrophages, monocytes, lymphocytes, and PMN were monitored over a 72-hour period for hemadsorption of chicken RBC. Hemadsorption increased for lung macrophages and monocytes, whereas it decreased for lymphocytes and PMN. Infective virus could not be recovered from PMN, RBC, lymphocytes, or monocytes for more than 24 hours after PI-3 infection. Recovery of infective PI-3 virus from infected peritoneal and lung macrophages extended over 4 to 8 days, respectively.  相似文献   

Identification of toxigenic Pasteurella multocida in atrophic rhinitis of pigs by in vitro characterisation     

GJ EAMENS  PD KIRKLAND  MJ TURNER  JA GARDNER†  MP WHITE‡  CL HORNITZKY 《Australian veterinary journal》1988,65(4):120-123

Toxigenic strains of Pasteurella multocida were readily differentiated from non-toxigenic strains by an agarose overlay method using bovine turbinate cells or bovine lung cells. Cells which were young and densely confluent were best suited to this assay. The incubation period required to distinguish toxigenic strains was dependent on the confluence of the monolayers, which was affected by the seeding rate, cell passage level and growth time prior to overlay. The agarose overlay method correctly identified 11 of 11 reference strains of Pasteurella multocida, and visible cytotoxic changes were present in the monolayers after 48 to 65 h. Outbreaks of the enzootic form of atrophic rhinitis in 2 New South Wales piggeries were associated with the isolation of toxigenic type D strains of P. multocida.  相似文献   

Concentration of Arachidonic Acid Metabolites in Arterial and Uterine Venous Blood During the Last Month of Pregnancy in Cows*     

M. Hoedemaker  P.G. Weston  W.C. Wagner 《Reproduction in domestic animals》1991,26(6):315-317

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Detection of Tritrichomonas foetus antigens in formalin-fixed, paraffin embedded sections by the peroxidase antiperoxidase technique     

CM CAMPERO  PW LADDS  RG HIRST  JA VAUGHAN  DE EMERY 《Australian veterinary journal》1989,66(8):264-266

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Use of hair root as a source of DNA for the detection of heterozygotes for recessive defects in cattle     

PJ HEALY  JA DENNIS  JF MOULE 《Australian veterinary journal》1995,72(10):392-392

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Idiopathic intranuclear inclusion bodies in the renal epithelium of macropods     

R. SPEARE  JA DONOVAN  LF SKERRATT  L. BERGER  PW LADDS  S.de  BEER 《Australian veterinary journal》1997,75(6):446-447

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Furazolidone toxicity in dairy calves     

JD TAYLOR  JA GIBSON  CEF YEATES 《Australian veterinary journal》1991,68(5):182-183

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Cardiomyopathy and woolly haircoat syndrome of Hereford cattle     

GJ STORIE  JA GIBSON  JD TAYLOR 《Australian veterinary journal》1991,68(3):119-119

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Progress in identifying and maintaining salmonella-free commercial chicken breeding flocks. 1. 1967-1969   总被引：1，自引：0，他引：1  

G H Snoeyenbos  B A McKie  C F Smyser  C R Weston 《Avian diseases》1970,14(4):683-696

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