Lythrum hyssopifolia (lesser loosestrife) poisoning of sheep in Victoria     

MJ Lancaster  JS Nimmo  C Lenghaus  IJ Gill  RD Crawford  RT Badman  JL Samuel  CJ Werner  C Button  N Kvalheim 《Australian veterinary journal》2009,87(12):476-479

Objective To document an ovine disease attributed to the consumption of Lythrum hyssopifolia (lesser loosestrife). Procedures Historical and histological review of field and experimental cases. Results 1–20% mortality occurred in sheep flocks grazing paddocks where L. hyssopifolia was the predominant green vegetation. Well‐documented disease outbreaks occurred in summer on nine farms across Victoria between 1974 and 2002. Liver damage occurred in all nine outbreaks, with kidney damage in at least eight. Hepatocyte necrosis was usually zonal to midzonal (zone 2) in the liver samples from four farms and periacinar (zone 3) in those from three farms, but some livers showed only single‐cell necrosis. Multinucleate hepatocytes near necrotic areas were a feature in six cases. Proximal tubular epithelium appeared to be the primary renal target and brown granules were often present in renal tubules. Biochemical and histological evidence of liver and kidney damage was obtained from two sheep experimentally pen‐fed harvested L. hyssopifolia. Conclusion Chemicals in L. hyssopifolia are toxic to ovine hepatocytes and renal tubular epithelial cells.  相似文献   

Quantification and comparison of wheat yield variation across space and time     

M.J. Florin  A.B. McBratney  B.M. Whelan 《European Journal of Agronomy》2009,30(3):212-219

Research about spatio-temporal variation of crop yield does not abound. From a precision agriculture (PA) perspective and particularly considering site-specific crop management (SSCM), this is an aberration. There is a serious need to further question how temporal variation of crop yield impacts ones ability to manage spatial variation. The aim of this study is to consider and develop new and existing approaches to this question. Spatio-temporal analysis was undertaken for two wheat fields in South Australia with 3 and 4 years of wheat yield data. Temporal analysis included the calculation of semi-variance across each field between pairs of years for the creation of maps and the calculation of rank correlations between pairs of years. These analyses supported previous notions that the magnitude of temporal variation is large compared with spatial variation. However, some consistence of spatial patterns between years was also observed for each of the fields indicating that considering magnitudes of variation alone is not an exhaustive analysis. A long-term (100 years) temporal analysis using variograms was undertaken for a single point simulated using the Agricultural Production Simulator Model (APSIM). The long-term analysis overcame the fact that 3 or 4 years of yield data are an extremely small sample size for the time dimension. This analysis provided some useful insight into temporal variation such as a large nugget variance accounting for 75% of the temporal variation and the cyclical nature of temporal yield variation. A novel use of pseudo cross semi-variograms was applied to a spatio-temporal analysis of yield variation for the two fields. This analysis provides a preliminary insight into identifying space–time variance equivalents. With greater depth of temporal crop yield data this is a promising perspective from which to identify optimal spatial management strategies.  相似文献   

Maturation of bovine dendritic cells by lipopeptides     

Hope JC  Whelan AO  Hewinson RG  Vordermeier M  Howard CJ 《Veterinary immunology and immunopathology》2003,95(1-2):21-31

The response of DC, and the subsequent stimulation of T cells, is an essential part of the initiation of immune responses following microbial challenge. The response of human DC to bacterial lipopeptides is mediated by toll-like receptor 2, and is characterised by DC maturation and the enhanced capacity to stimulate of T cells. We report here that bovine DC are also induced to mature following lipopeptide stimulation. Exposure of DC to the model lipopeptide Pam3CSK4 was associated with increased expression of MHC, costimulatory molecules, and enhanced secretion of IL-12 and TNFalpha. Lipopeptide-matured DC were superior in their ability to induce T cell activation and IFNgamma secretion. In contrast, exposure of MPhi to lipopeptides induced down-regulation of MHC expression and much lower increases in IL-12 secretion. A lipopeptide derived from the sequence of a relevant mycobacterial lipoprotein, MPB83, also influenced bovine DC by stimulating increases in IL-12 and TNFalpha secretion. These different changes in bovine DC and MPhi may have important implications for immune responses induced following bacterial infection with uptake of microbes by DC resulting in potentiation of their immunostimulatory capacity and uptake by MPhi having a much less marked effect on immune responses.  相似文献   

Characterisation of Pasteurella multocida isolated from fowl cholera outbreaks on turkey farms     

PJ BLACKALL  JL PAHOFF  D MARKS†  N FEGAN  CJ MORROW‡ 《Australian veterinary journal》1995,72(4):135-138

SUMMARY: Biochemical profiles, restriction endonuclease analysis (REA) and ribotyping were used to investigate Pasteurella multocida isolates from outbreaks of fowl cholera on 7 turkey farms in New South Wales. While only a single isolate was available from 5 of the farms, multiple isolates, 4 and 12 respectively, were available from the other 2 farms. The available field evidence suggested that 8 outbreaks had occurred with one farm suffering 2 outbreaks. The isolates obtained were all confirmed as Pasteurella multocida . Biochemical profiles allocated the isolates to 4 groups, 3 being variants of P multocida subsp multocida and the fourth being P multocida subsp septica . REA performed with Hpall established 7 groups. Ribotyping using the Hpall digests probed with the 16S rRNA operon of Haemophilus paragallinarum recognised the same 7 groups as REA. Unlike the biochemical profiles, both REA and ribotyping provided a fine subdivision that identified outbreaks as either related or unrelated. The REA and ribotyping patterns as well as biochemical profiles were stable for all isolates from the outbreaks in which multiple isolates were obtained from either the same bird or from different birds. REA and ribotyping were found to be superior to biotyping methods for the investigation of fowl cholera outbreaks.  相似文献   

Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection     

Chandran K  Sullivan NJ  Felbor U  Whelan SP  Cunningham JM 《Science (New York, N.Y.)》2005,308(5728):1643-1645

Ebola virus (EboV) causes rapidly fatal hemorrhagic fever in humans and there is currently no effective treatment. We found that the infection of African green monkey kidney (Vero) cells by vesicular stomatitis viruses bearing the EboV glycoprotein (GP) requires the activity of endosomal cysteine proteases. Using selective protease inhibitors and protease-deficient cell lines, we identified an essential role for cathepsin B (CatB) and an accessory role for cathepsin L (CatL) in EboV GP-dependent entry. Biochemical studies demonstrate that CatB and CatL mediate entry by carrying out proteolysis of the EboV GP subunit GP1 and support a multistep mechanism that explains the relative contributions of these enzymes to infection. CatB and CatB/CatL inhibitors diminish the multiplication of infectious EboV-Zaire in cultured cells and may merit investigation as anti-EboV drugs.  相似文献   

Survey of vets employed in industry     

Sheridan L  Whelan K 《The Veterinary record》2011,168(20):543-544

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Sleep aid toxicosis in dogs: 317 cases (2004–2010)     

Adam R. Lancaster DVM  Justine A. Lee DVM  DACVECC  Lynn R. Hovda RPH  DVM  MS  DACVIM  Brian T. Hardy DVM  Lee X. Miyahara VMD  Elizabeth P. Martin VMD  Megan F. Whelan DVM  DACVECC 《Journal of Veterinary Emergency and Critical Care》2011,21(6):658-665

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A comparison of the pathogenicity of two strains of hog cholera virus: 2. Virological studies     

S. KAMOLSIRIPRICHAIPORN  CJ MORRISSY  HA WESTBURY 《Australian veterinary journal》1992,69(10):245-248

Quantitative and qualitative differences were demonstrated in the amount of virus in a range of tissues from pigs infected with either the Weybridge or New South Wales (NSW) strains of hog cholera (HC) virus. The titre of the Weybridge strain in samples, as assessed by either virus titration in cell culture or by the density of specific fluorescing cells in tissue sections, was higher than that for the NSW strain. This correlated with the greater severity of the clinico-pathological syndrome induced by the Weybridge strain. The implications of the differences in the virus content of tissues in the diagnosis of HC is discussed as is the use of monoclonal antibodies to differentiate HC and bovine virus diarrhoea viruses.  相似文献   

A critique of the Texas A&M model when used to simulate beef cattle grazing pasture     

M.B. Whelan  E.J.A. Späth  F.H.W. Morley 《Agricultural Systems》1984,14(2):81-84

This paper describes the experiences of the authors with the Texas A&M University model when used to simulate the production of beef cattle in a grazing system. Problems associated with the lack of interaction between pasture and animals, including the monthly time interval, supplementation and an input parameter (weight at maturity), are discussed.  相似文献   

Efficacy of erythromycin compared with penicillin/streptomycin for the treatment of virulent footrot in sheep   总被引：2，自引：0，他引：2  

JK WEBB WARE  CJ SCRIVENER  AL VIZARD 《Australian veterinary journal》1994,71(3):88-89

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