全文获取类型
收费全文 | 14999篇 |
免费 | 1172篇 |
国内免费 | 1210篇 |
专业分类
林业 | 1352篇 |
农学 | 1651篇 |
基础科学 | 1086篇 |
2224篇 | |
综合类 | 4504篇 |
农作物 | 916篇 |
水产渔业 | 846篇 |
畜牧兽医 | 3031篇 |
园艺 | 636篇 |
植物保护 | 1135篇 |
出版年
2024年 | 38篇 |
2023年 | 221篇 |
2022年 | 387篇 |
2021年 | 462篇 |
2020年 | 501篇 |
2019年 | 548篇 |
2018年 | 485篇 |
2017年 | 599篇 |
2016年 | 553篇 |
2015年 | 688篇 |
2014年 | 683篇 |
2013年 | 816篇 |
2012年 | 1021篇 |
2011年 | 1021篇 |
2010年 | 954篇 |
2009年 | 875篇 |
2008年 | 929篇 |
2007年 | 862篇 |
2006年 | 713篇 |
2005年 | 666篇 |
2004年 | 386篇 |
2003年 | 435篇 |
2002年 | 447篇 |
2001年 | 554篇 |
2000年 | 460篇 |
1999年 | 343篇 |
1998年 | 215篇 |
1997年 | 181篇 |
1996年 | 183篇 |
1995年 | 160篇 |
1994年 | 149篇 |
1993年 | 112篇 |
1992年 | 125篇 |
1991年 | 105篇 |
1990年 | 103篇 |
1989年 | 76篇 |
1988年 | 63篇 |
1987年 | 41篇 |
1986年 | 28篇 |
1985年 | 23篇 |
1984年 | 20篇 |
1983年 | 16篇 |
1982年 | 11篇 |
1981年 | 8篇 |
1979年 | 9篇 |
1969年 | 5篇 |
1966年 | 6篇 |
1956年 | 6篇 |
1893年 | 4篇 |
1892年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
51.
52.
53.
54.
55.
Y. J. Huang C. Toscano-Underwood B. D. L. Fitt † X. J. Hu A. M. Hall 《Plant pathology》2003,52(2):245-255
Ascospores of both A-group and B-group Leptosphaeria maculans germinated at temperatures from 5 to 20°C on leaves of oilseed rape. Germination of ascospores of both groups started 2 h after inoculation and percentage germination reached its maximum about 14 h after inoculation at all temperatures. Both the percentage of A-/B-group ascospores that had germinated after 24 h incubation and germ tube length increased with increasing temperature from 5 to 20°C. Germ tubes from B-group ascospores were longer than those from A-group ascospores at all temperatures, with the greatest difference at 20°C. Hyphae from ascospores of both groups penetrated the leaves predominantly through stomata, at temperatures from 5 to 20°C. A-group ascospores produced highly branched hyphae that grew tortuously, whereas B-group ascospores produced long, straight hyphae. The percentage of germinated ascospores that penetrated stomata increased with increasing temperature from 5 to 20°C and was greater for A-group than for B-group L. maculans after 40 h incubation. 相似文献
56.
A. van Maanen X.-M. Xu 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(7):669-682
An epidemic is the progress of disease in time and space. Each epidemic has a structure whose temporal dynamics and spatial patterns are jointly determined by the pathosystem characteristics and environmental conditions. One of the important objectives in epidemiology is to understand such spatio-temporal dynamics via mathematical and statistical modelling. In this paper, we outline common methodologies that are used to quantify and model spatio-temporal dynamics of plant diseases, with emphasis on developing temporal forecast models and on quantifying spatial patterns. Several examples of epidemiological models in cereal crops are described, including one for Fusarium head blight. 相似文献
57.
Rapid detection of Phytophthora cinnamomi using PCR with primers derived from the Lpv putative storage protein genes 总被引:1,自引:1,他引:1
Phytophthora cinnamomi is an ecologically and economically important pathogen. In this study, PCR assays were developed with primer pair LPV2 or LPV3 for rapid detection and identification of this organism. Both primer pairs were selected from putative storage protein genes. The specificity of these primer pairs was evaluated against 49 isolates of P. cinnamomi , 102 isolates from 30 other Phytophthora spp., 17 isolates from nine Pythium spp. and 43 isolates of other water moulds, bacteria and true fungi. PCR with both primer pairs amplified the DNA from all isolates of P. cinnamomi regardless of origin. The LPV3 primers showed adequate specificity among all other species tested. The LPV2 primers cross-reacted with some species of Pythium and true fungi, but not with any other Phytophthora species. PCR with the LPV3 primers detected the pathogen at levels of a single chlamydospore or 10 zoospores in repeated tests. The PCR assay was at least 10 times more sensitive than the plating method for detection of the pathogen from artificially infested soilless medium, and, to a lesser extent, from naturally infected plants. PCR with LPV3 primers can be a useful tool for detecting P. cinnamomi from soilless media and plant tissues at ornamental nurseries, whereas the LPV2 primers can be an effective alternative for identification of this species from pure culture. Applications of these assays for detection of P. cinnamomi in other environments were also discussed. 相似文献
58.
大豆疫病的种子处理技术研究 总被引:2,自引:0,他引:2
本试验用不同浓度的瑞毒锰锌、杀毒矾和克露进行大豆种子处理试验,结果表明,500ppm瑞毒锰锌对大豆疫病具有很好的治疗作用,而用杀毒矾以种子重量0.4—0.5%的剂量闷种,能显著地抑制大豆疫霉菌的侵入 相似文献
59.
分析植物酸性转化酶基因的保守区序列,设计一对PCR引物,以君子兰基因组DNA为模板,采用PCR方法扩增出长约500 bp的DNA片段,克隆入pGEM-TEasy载体,测序结果表明获得君子兰酸性转化酶基因家族的一个成员CMCW1,该基因片段长518 bp,不含内含子,编码172个氨基酸。其序列已在GenBank中登记(登记号为AY151269)。在GenBank中进行同源性检索的结果表明,该成员编码的氨基酸与其它植物细胞壁酸性转化酶编码的氨基酸同源性较高。 相似文献
60.
AIM: To explore interaction and biological behaviour changes of two kinds of cells-blastocysts and hepatocarcinoma cells in the same microenvironment. METHODS:The models of mouse blastocysts co-cultured with human hepatocarcinoma cell lines were established, then biological behaviours and mutual effects of the two kinds of cells in co-culture system were observed. RESULTS: Compared with control group, hepatocarcinoma cells with differently invasive and metastatic potential significantly enhanced the rates of blastocyst hatchment , attachment and outgrowth(P<0.05). There was no significant difference in those among hepatocarcinoma cells co-cultured groups (P>0.05). The blastocyst hatched and attached to hepatocarcinoma cells with differently invasive and metastatic potential. Then, differential trophoblasts invaded hepatocarcinoma cells. The clear-cut interfaces were gradually formed between both sides. Hepatocarcinoma cells on interface showed changes of growth direction and cell shapes and did not invade blastocysts. CONCLUSIONS: Hepatocarcinoma cells promoted blastocyst development. Blastocysts implanted and invaded hepatocarcinoma cells with differently invasive and metastatic potential in vitro, which indicate that blastocyst implantation in vitro does not relate with the kinds and differential level of interactional cells and the low selectivity maybe relate with high adaptability of early life. 相似文献