Effect of hypercapnia on lysyl oxidase-dependent collagen cross-linking and hypoxia-induced pulmonary hypertension in rat     

CHEN Wei-qian  PENG Yan-ping  ZHANG Wei-xi  YE Le-ping  DONG Liang  XIA Xiao-dong 《园艺学报》2017,33(8):1481-1486

AIM: To investigate the effect of hypercapnia on hypoxia-induced pulmonary hypertension and the changes of lysyl oxidase (LOX) and extracellular matrix collagen cross-links in the rat. METHODS: Sprague-Dawley rats were randomly divided into 4 groups:normoxia group, hypoxia group, hypercapnia group and hypoxia+hypercapnia group. LOX activity was detected by fluorescence spectrophotometry. LOX protein expression was detected by immunohistochemistry and Western blot. The mRNA expression of LOX in the pulmonary artery was detected by real-time PCR. RESULTS: The levels of mean pulmonary artery pressure (mPAP), RV/(LV+S) and WA/TA in hypoxia group were significantly higher than those in normoxia group (P<0.01). Moreover, the levels of mPAP and RV/(LV+S) in hypoxia+hypercapnia group were significantly lower than those in hypoxia group (P<0.01). However, no significant difference of mPAP and RV/(LV+S) between hypercapnia group and normoxia group was observed. In hypoxia group, the collagen cross-links in the lung tissue was significantly higher than that in normoxia group and hypercapnia group (P<0.01). Importantly, collagen cross-links in the lung tissue of hypoxia+hypercapnia group was significantly lower than that in hypoxia group (P<0.01). There was no significant difference in collagen cross-links between hypercapnia group and normoxia group. The expression of LOX at mRNA and protein levels and its activity in the pulmonary arteries of hypoxia group were significantly increased as compared with normoxia group (P<0.01). Furthermore, the expression of LOX at mRNA and protein levels and its activity in the pulmonary arteries in hypoxia+hypercapnia group were lower than those in hypoxia group (P<0.01). CONCLUSION: Hypoxia not only up-regulates LOX but also promotes collagen cross-linking in the rat lung, which contributes to the development of pulmonary hypertension. Hypercapnia inhibits hypoxia-induced LOX expression and collagen cross-linking, therefore impairing the progress in hypoxia-induced pulmonary hypertension.  相似文献   

Dexmedetomidine attenuates acute alcoholic hepatic injury in mice     

HUANG Qiang  XIA Ming-zhu  YANG Wen-jie  DAI Zhong-liang  JIANG Yuan-xu 《园艺学报》2017,33(10):1891-1895

AIM: To investigate the effects of dexmedetomidine (DEX) on acute alcoholic hepatic injury in mice and to explore the possible mechanisms. METHODS: Kunming mice (n=50) were randomly divided into 5 groups (n=10): normal saline control (NS) group, acute alcoholic hepatic injury model (E) group, low-dose (10 μg/kg) DEX (E+L) group, medium-dose (50 μg/kg) DEX (E+M) group and high-dose (100 μg/kg) DEX (E+H) group. The animals were sacrificed at 6 h after gavage of alcohol or normal saline. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) were measured. The livers were removed for evaluation of histological characteristics and determining the content of tumor necrosis factor-α (TNF-α) amd interleukin-1β (IL-1β) in the liver tissues by ELISA. The expression levels of cytochrome P450 2E1 (CYP2E1) and nuclear factor-κB (NF-κB) in the liver tissues were evaluated by Western blot. RESULTS: Compared with NS group, the levels of ALT, AST and TG were obviously increased in E group, which were obviously decreased in E+M and E+H groups. Compared with NS group, the levels of TNF-α, IL-1β and MDA were obviously increase in E group, which were obviously decreased in E+M and E+H groups. Compared with NS group, the activity of SOD and the content of GSH were obviously decreased in E group, which were obviously increased in E+M and E+H groups. Compared with NS group, the expression of CYP2E1 and NF-κB was obviously increase in E group, which was obviously decreased in E+M and E+H groups. Compared with NS group, ethanol induced marked liver histological injury, which was less pronounced in E+M and E+H groups. CONCLUSION: DEX has a protective effect on mouse liver with acute alcoholic injury by the involvement in the processes of antioxidation and antiinflammation, and its mechanism may be associated with the inhibition of CYP2E1 and NF-κB expression.  相似文献   

Screening of lentiviral vectors carrying effective siRNA targeting S1P2 gene     

YAN Xiao  XIA Ji-yi  CHENG Bo  ZHU Yong-sheng  JIANG Rui 《园艺学报》2017,33(7):1338-1344

AIM:To screen the lentiviral vector carrying siRNA with higher efficiency of suppressing the sphingosine-1-phosphate receptor 2(S1P2) gene expression in the primarily cultured corpus cavernosum smooth muscle cells of spontaneously hypertensive rats (SHR).METHODS:SHR and SD rats (n=5 each) were used for primarily culturing corpus cavernosum smooth muscle cells.The cells were randomly divided into 6 groups:SHR siRNA-1,SHR siRNA-2,SHR siRNA-3,SHR GFP,SHR control (SHR non-transfection group),and SD control (SD rat control group).Each group had 5 samples with 1.0×10⁵ cells of each sample.At 72 h after transfection (MOI=60) with lentiviral vectors carrying S1P2 siRNA into the SHR corpus cavernosum smooth muscle cells,the expression of GFP was observed under fluorescence microscope.The protein expression of S1P2,ROCK1,ROCK2 and eNOS in the corpus cavernosum smooth muscle cells,and the mRNA expression of S1P2,ROCK1 and ROCK2 were determined by by Western blot and RT-PCR.RESULTS:The transfection efficiency of the corpus cavernosum smooth muscle cells in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were>80%.Compared with SHR control group,the mRNA levels and the protein expression of S1P2,ROCK1 and ROCK2 in SHR GFP group showed no remarkable changes,while those in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SD control groups were significantly lower than those in SHR control group (P<0.05).The protein expression of eNOS in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were not significantly changed as compared with SHR control group,but that in SD control group was significantly higher than that in SHR control group.CONCLUSION:Three groups of siRNA lentiviral vectors targeting S1P2 inhibit the expression of S1P2 in the corpus cavernosum smooth muscle cells of SHR,and by silencing the S1P2 expression,the expression of ROCK1 and ROCK2 is inhibited.Among them,siRNA-1 has the highest inhibitory efficiency.  相似文献   

基于生物催化法的食品熏蒸剂环氧乙烷制备     

徐宁  辛嘉英  王艳  窦博鑫  夏春谷 《农业机械学报》2017,48(2):322-326,321

为了探索一种反应条件温和、方法简单的食品熏蒸剂环氧乙烷制备方法,采用甲基弯菌IMV 3011(Methylosinus trichosporium IMV 3011)细胞作为生物催化剂,催化乙烯的环氧化反应生成环氧乙烷,确定了反应条件。结果表明:反应器中气相的组成为氧气50%、乙烯20%、氮气30%(体积分数),30℃、150 r/min振荡反应8 h,采用固定化形式催化剂,环氧乙烷生成量为34μmol/mg,利用甲烷培养对其循环再生,再生8次催化剂中甲烷单加氧酶(MMO)活力仍保留89%,环氧乙烷物质的量为3.4 nmol。  相似文献   

氮肥运筹对钵苗机插优质食味水稻产量及品质的影响   总被引：11，自引：0，他引：11  

胡群  夏敏  张洪程  曹利强  郭保卫  魏海燕  陈厚存  韩宝富 《作物学报》2017,43(3):420-431

以江苏优质食味水稻代表性品种南粳9108和南粳5055为材料,采用新型栽培技术钵苗机插移栽,在大田常用量总施氮270 kg hm~(–2)条件下,设置10∶0、9∶1、8∶2、7∶3、6∶4、5∶5、4∶6七种基蘖肥与穗肥比例的处理。比较研究不同处理对钵苗机插优质食味水稻产量及稻米品质的影响,以期为氮肥的合理施用提供理论依据和技术指导。结果表明,当基蘖肥与穗肥比例为6∶4时,产量最高,与其他处理差异显著。稻米的糙米率、精米率和整精米率均随着基蘖肥比例的降低而逐渐增加;直链淀粉和胶稠度含量随着基蘖肥比例的降低而逐渐减小,但蛋白质的含量变化趋势相反,后期施氮量大时蛋白质含量增大。两品种的垩白率和垩白度均随着基蘖肥比例的降低呈先增加后减小的趋势,以6∶4处理显著大于其他处理,而垩白大小逐渐增加。食味品质中除了完整性随着基蘖肥所占比例的降低而增加外,香气、光泽、味道、口感以及食味值均呈现递减趋势。各处理的稻米峰值黏度、热浆黏度、崩解值和最终黏度,除对照不施氮肥处理最大外,均随基蘖肥比例的降低而逐渐减小,而消减值的变化呈现相反的趋势。基蘖肥与穗肥比例为6∶4的氮肥运筹方式能显著提高钵苗机插优质食味水稻的产量;适当增加穗肥比例能有效改善稻米的加工品质和营养品质,增大稻米粒长和粒宽,但降低了稻米的外观品质和食味品质。  相似文献   

光照对沙葱种子萌发及抗氧化代谢的影响     

郭霏  鄂圆圆  杨忠仁  郝丽珍  张凤兰  朱文峰  夏峰 《种子》2017,(8):28-31

研究了光照对沙葱种子萌发及抗氧化代谢的影响,结果表明,12h光照和24 h光照下的沙葱种子发芽率仅为对照的20.8％和8.8％,表明沙葱存在光休眠现象,为需暗种子;在12 h和24 h光照处理下,催芽前期SOD、POD活性及Vc含量水平较高,MDA和CAT含量低于0h;而在种子萌发后期,SOD、POD活性降低,MDA和CAT含量升高,说明在光胁迫下,沙葱种子萌发后期的膜脂过氧化作用增强.  相似文献   

楠木枝枯病病原菌鉴定     

岳阳  吴利平  夏俊勇  马良进 《植物病理学报》2019,49(5):699-704

<正>楠木为我国传统名贵树种,其木质紧密、耐腐蚀性强,能够驱虫美化环境,是建筑、家具、雕刻的首选木料;同时具有净化空气、吸烟滞尘、涵养水源、固土防沙等功能,有着巨大的经济及生态价值。楠木主要包括润楠属(Machilus Nees)以及楠属(Phoebe Nees)植株,均属于濒危树种,在我国境内  相似文献   

"沃得天"微肥叶面喷施对猕猴桃产量和品质的影响     

杨畅  夏婕  李秀双  陈艳龙  田霄鸿 《干旱地区农业研究》2019,37(6):193-198

验证"沃得天"微肥(一种新型微量元素水溶肥)对猕猴桃果树的喷施肥效,为陕西省猕猴桃生产提质增效提供科学依据。试验设置了喷施新型微量元素水溶肥("沃得天"微肥1(W1)、"沃得天"微肥2(W2))、普通微量元素水溶肥(G1)、清水(当地地下水,CK1)4个处理,供试材料为"秦美"猕猴桃,田间采用完全随机区组设计,测定了叶片生长、果实产量和品质方面的指标,并综合评价了"沃得天"微肥的田间应用效果。结果表明,与对照相比,喷施W1和W2均显著提高了猕猴桃叶片的百叶重和百叶厚,百叶重分别增加22.6%和33.3%,百叶厚分别增加20%和27%;其中W2还能同时增加果实横、纵径,增幅为11.0%和12.9%,并能够显著提高叶片中Cu、Zn、Fe、Mn以及Ca的含量,其中与普通微肥相比增加Fe元素60～78 mg·kg~(-1)。喷施W1和W2分别增加猕猴桃单果重为11.3%和8.8%,增加了果实的固酸比,其中W2果实中Vc的含量显著增加了8.6%;W1和W2还不同程度增加了果实中多种微量元素和钙的含量,优化了猕猴桃果实外观和营养品质。另外,W1和W2处理猕猴桃分别增产8.11%和5.17%,增益8.83%和5.43%,而且2种"沃得天"新型微量元素肥料对猕猴桃增产提质效果均优于市售普通微肥。因此,叶面喷施"沃得天"新型微肥可以促进陕西"秦美"猕猴桃的生长发育,并且能在一定程度上调控叶片和果实对必需微量元素的累积,在提高果实品质和增产增收及优化猕猴桃肥料管理方面效果显著。  相似文献   

中国大陆地区基于太阳辐射经验值计算ET₀的适用性研究     

夏兴生  朱秀芳  潘耀忠  张锦水 《干旱地区农业研究》2019,37(6):221-230

太阳辐射是利用FAO推荐的Penman-Monteith(PM)公式计算参考作物需水量(ET_0)的必要参数。为了探究PM公式在辐射数据缺失的条件下,利用FAO推荐的公式及参数获得太阳辐射值(R_(s_c))替代观测值(R_(s_o))在中国大陆地区的适用性,本研究选用了中国大陆112个站点至少15 a的多年月平均观测数据,通过逐点计算分析了R_(s_c)和R_(s_o)的时空差异及二者分别输入PM公式获得的参考作物需水量ET_(0_c)和ET_(0_o)的时空差异。结果表明,R_(s_c)与R_(s_o)存在显著的时空差异性,二者相对差值范围为-2.86～4.41 MJ·m~(-2)·d~(-1),且在4—8月份差异较大;大致以"胡焕庸线"为界,线西北区域R_(s_c)与R_(s_o)的时空差异相对较小,且稳定,线东南区域的时空差异较大,且不稳定。但是,基于二者计算的ET_(0_c)和ET_(0_o)时空差异却不显著,平均只有0.06～0.26 mm·d~(-1)的误差;"胡焕庸线"西北地区的ET_(0_c)和ET_(0_o)绝对差值常年稳定在0.00～0.25 mm·d~(-1),"胡焕庸线"线东南地区则随季节而变化,夏季差异相对较大。在实际的应用中,西北地区全年和北方地区春、秋、冬三季以及长江、珠江流域所覆盖的南方地区在1、2、10、11、12月使用R_(s_c)替代R_(s_o)获得ET_0具有较好的适用性,北方地区的夏季、南方地区的3—9月份使用R_(s_c)计算ET_0则必须研究相应的方法对结果进行矫正,否则会有误差,且偏大。  相似文献   

红颈常室茧蜂雌性生殖系统及卵巢管形态学观察          下载免费PDF全文

夏亚运  田苗苗  李明明  毛建军  蔡笃程  罗淑萍 《中国生物防治学报》2019,35(4):517-526

利用光学显微镜和透射电镜观察红颈常室茧蜂Peristenus spretus Chen et van Achterberg雌性生殖系统结构、卵巢发育和卵子发生、卵子超微结构,并对15℃和23℃饲养条件下不同日龄的雌蜂卵巢管长度、卵巢管内卵室大小与成熟度进行了比较,揭示了该茧蜂雌性生殖系统结构与特征。结果表明：1）红颈常室茧蜂生殖系统结构主要包括一对卵巢、2条输卵管、中输卵管等;2）雌成虫在羽化前期（1～3 d）卵巢内的成熟卵粒较少,卵巢管内含有大量的卵原细胞,卵子内充满了大量的脂滴,中后期（5～11 d）随着脂肪体的降解吸收,卵子内逐渐新生一些线粒体、高尔基体等细胞器,其内存在滋养细胞、卵母细胞,并且滋养细胞无细胞膜存在。3）同一温度不同日龄红颈常室茧蜂卵粒数、成熟卵子数均差异显著,23℃下红颈常室茧蜂每条卵巢管内的日均卵子数20.70粒,显著高于15℃的日均卵子数18.65粒。  相似文献