全文获取类型
收费全文 | 25646篇 |
免费 | 1785篇 |
国内免费 | 1426篇 |
专业分类
林业 | 1887篇 |
农学 | 2411篇 |
基础科学 | 831篇 |
3514篇 | |
综合类 | 5873篇 |
农作物 | 1419篇 |
水产渔业 | 1621篇 |
畜牧兽医 | 8796篇 |
园艺 | 664篇 |
植物保护 | 1841篇 |
出版年
2023年 | 268篇 |
2022年 | 438篇 |
2021年 | 559篇 |
2020年 | 633篇 |
2019年 | 687篇 |
2018年 | 662篇 |
2017年 | 780篇 |
2016年 | 741篇 |
2015年 | 838篇 |
2014年 | 929篇 |
2013年 | 1196篇 |
2012年 | 1224篇 |
2011年 | 1391篇 |
2010年 | 1230篇 |
2009年 | 1079篇 |
2008年 | 1132篇 |
2007年 | 1054篇 |
2006年 | 935篇 |
2005年 | 991篇 |
2004年 | 707篇 |
2003年 | 879篇 |
2002年 | 1067篇 |
2001年 | 1327篇 |
2000年 | 1118篇 |
1999年 | 839篇 |
1998年 | 346篇 |
1997年 | 300篇 |
1996年 | 249篇 |
1995年 | 255篇 |
1994年 | 229篇 |
1993年 | 203篇 |
1992年 | 418篇 |
1991年 | 424篇 |
1990年 | 354篇 |
1989年 | 314篇 |
1988年 | 302篇 |
1987年 | 341篇 |
1986年 | 255篇 |
1985年 | 197篇 |
1984年 | 177篇 |
1983年 | 150篇 |
1982年 | 98篇 |
1981年 | 88篇 |
1980年 | 81篇 |
1979年 | 142篇 |
1978年 | 94篇 |
1977年 | 73篇 |
1975年 | 77篇 |
1974年 | 79篇 |
1969年 | 70篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
51.
青藏高原西部狮泉河宽谷区的荒漠化过程 总被引:2,自引:0,他引:2
狮泉河宽谷区的荒漠化过程以整个青藏高原的环境演化为背景,受人为的资源利用不合理而强化。今天的宽谷景观一方面隐含了高原宽谷的变化,一方面显示出干旱,退化的主导特征,较之西北干旱区,生态系统更具脆弱性,荒漠化的治理应在发挥区内水资源优质的同时强调生态系统自身的稳定。 相似文献
52.
53.
S Katsuda H Hosomi M Shiomi Y Watanabe 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(4):669-673
Effects of atherosclerosis on the mean value and daily variation of arterial pressure were studied in 12 Watanabe-heritable hyperlipidemic (WHHL) rabbits aged 12 to 35 months and 25 normal Japanese white rabbits aged 6 to 30 months. A pressure catheter was inserted through the left subclavian artery under pentobarbital anesthesia. A few days after the catheterization, the mean arterial pressure (MAP) of the rabbits, which were active and in a good state of appetite, was recorded by an analogue-to-digital converter every second for about 6 hrs and stored in a computer. The mean (M) and standard deviation (SD) in the WHHL rabbit, calculated from each successive MAP record, ranged widely from 85.8 to 131.4 mmHg and 5.6 to 12.6 mmHg, respectively. There was no significant correlation between M and SD in the WHHL rabbit. M and variance (V) of MAP in the WHHL rabbit were significantly higher than those in the normal rabbit. M did not show any significant change with increasing ages, whereas SD increased significantly with aging in the WHHL rabbit. Concentrations of serum total cholesterol and triglyceride in the WHHL rabbit were 475 and 328 mg/dl, which were about nine and seven times as high as those in the normal rabbit, respectively. Macroscopic and histopathological examinations of the aorta revealed development and spread of sclerotic lesions with aging in the WHHL rabbit. We can conclude that development of atherosclerosis with aging in the WHHL rabbit causes malfunction of the baroreceptors, which contributes to hypertension and lability of arterial pressure. 相似文献
54.
55.
T Tsuboi Y Kanazawa T Syoji S Tokuhisa 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(6):1179-1181
Bovine follicular oocytes collected from bovine ovaries were exposed to bovid herpesvirus 1 (BHV-1). After washings, these oocytes were cultured to mature. As a result BHV-1 could not be removed from the oocytes and could replicate in the oocytes with cumulus cells, but not in the oocytes without the cells. Moreover, the specific fluorescence for BHV-1 was detected in the cumulus cells by a indirect immunofluorescent technique. Therefore these findings suggested BHV-1 could be absorbed in the oocytes but the replication of BHV-1 was done in the cumulus cells. 相似文献
56.
K Adachi A Yoshimoto T Hasegawa T Shimizu Y Goto S Makimura 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(6):1081-1084
Due to the potential for anti-erythrocyte membrane antibodies as possible enhancers of erythrocyte destruction, the presence of serum anti-erythrocyte membrane antibodies in 31 dogs with Babesia gibsoni infection admitted to a veterinary hospital was investigated by an enzyme linked immunosorbent assay (ELISA) and immunoblotting analyses. This infection resulted in an increase of anti-erythrocyte membrane antibodies in 84% (IgG) and 74% (IgM) of 31 infected dogs, respectively. This was confirmed by the similarity in the protein profiles of the erythrocyte membrane antigens immunoblotted with rabbit antiserum to dog erythrocyte membrane antigens and infected dog serum. These results suggest the production of anti-erythrocyte membrane antibodies was induced by B. gibsoni infection. 相似文献
57.
民本思想是中国古代治国的基本指导思想,在民和天、民和国、民和君的关系问题上,明确提出的“民为神主”、“民为邦本”、“民贵君轻”等朴素唯物主义观点,对于我党坚持群众路线,加强自身建设,加快社会主义民主政治建设具有重要的现实意义。 相似文献
58.
Kano R. Kubota A. Nakamura Y. Watanabe S. Hasegawa A. 《Veterinary research communications》2001,25(8):615-622
Using cDNA from a CRFK cell line as a template, PCR amplification was performed with the Ub1S and poly(dT) primers to isolate feline ubiquitin genes. Sequencing of the 495 bp PCR fragment revealed that the putative amino acids induced by this fragment gave a fusion protein consisting of a ubiquitin polypeptide (76 amino acids) and an extension protein of ribosomal proteins L40 (52 amino acids). The putative amino acid sequence of ubiquitin was identical to those of humans, rats and pigs.The recombinant glutathione S-transferase (GST)–feline ubiquitin fusion proteins were produced in Escherichia coli and purified. The fusion proteins had a molecular weight of about 42 kDa and were detected by immunoblot assay with rabbit anti-ubiquitin antiserum.The mRNAs from heat-shocked and non-heat-shocked cells were subjected to RT-PCR (Ub1S and poly(dT) primers) analysis. The molecular weights of the ubiquitinated proteins in heat-shocked CFRK cells were between 18 kDa and 24 kDa by immunoblot assay.These results suggested that there were more ubiquinated proteins in the heat-shocked CRFK cells than in the pre-heat-shocked cells. 相似文献
59.
60.
Characterisation of cross-reactivity of virus neutralising antibodies induced by feline panleukopenia virus and canine parvoviruses 总被引:1,自引:0,他引:1
K. Nakamura Y. Ikeda T. Miyazawa Y. Tohya E. Takahashi M. Mochizuki 《Research in veterinary science》2001,71(3):219-222
It was recently reported that canine parvoviruses (CPV) had entered cat populations and induced disease in infected cats, while they had affected only dogs in the past. It is important to determine whether conventional feline panleukopenia virus (FPLV) vaccines protect against recent CPV infections. In this study, the cross-reactivity of virus-neutralising (VN) and haemagglutinin-inhibition (HI) antibodies in cats induced by FPLV and CPV s were examined. Lower cross-reactivities of VN and HI antibodies against each CPV strain were observed in cats experimentally inoculated with FPLV or vaccinated with an inactivated FPLV vaccine. In addition, we revealed the existence of a novel type of FPLV, which reacted weakly with antibodies induced by the conventional FPLV vaccine. 相似文献