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Construction of Genetic Linkage Map Based on SSR Markers in Peanut(Arachis hypogaea L.) 总被引:1,自引:0,他引:1
HONG Yan-bin LIANG Xuan-qiang CHEN Xiao-ping LIU Hai-yan ZHOU Gui-yuan LI Shao-xiong WEN Shi-jie 《中国农业科学(英文版)》2008,7(8)
Molecular genetic maps of crop species can be used in a variety of ways in breeding and genomic research such as identification and mapping of genes and quantitative trait loci (QTLs) for morphological, physiological and economic traits of crop species. However, a comprehensive genetic linkage map for cultivated peanut has not yet been developed due to the extremely low frequency of DNA polymorphism in cultivated peanut. In this study, 142 recombinant inbred lines (RILs) derived from a cross between Yueyou 13 and Zhenzhuhei were used as mapping population in peanut (Arachis hypogaea L.). A total 652 pairs of genomic-SSR primer and 392 pairs of EST-SSR primer were used to detect the polymorphisms between the two parents. 141 SSR primer pairs, 127 genomic-SSR and 14 EST-SSR ones, which can be used to detect polymorphisms between the two parents, were selected to analyze the RILs population. Thus, a linkage genetic map which consists of 131 SSR loci in 20 linkage groups, with a coverage of 679 cM and an average of 6.12 cM of inter-maker distance was constructed. The putative functions of 12 EST-SSR markers located on the map were analyzed. Eleven showed homology to gene sequences deposited in GenBank. This is the first report of construction of a comprehensive genetic map with SSR markers in peanut (Arachis hypogaea L.). The map presented here will provide a genetic framework for mapping the qualitative and quantitative trait in peanut. 相似文献
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花生细胞工程与遗传转化研究进展 总被引:2,自引:0,他引:2
花生细胞工程与遗传转化的研究对于花生品种改良、新品种繁育以及种质资源保存等具有重要意兰。对二十世纪90年代以来花生高效再生技术和遗传转化研究的现状以及发展趋势进行概述和讨论。 相似文献
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广东花生产区土壤黄曲霉产毒菌落分布初步研究 总被引:2,自引:0,他引:2
采用稀释倒平板法对广东10个花生主产地土壤中黄曲霉的数量以及分离菌株的产毒能力进行了分析。结果表明:全省各地花生种植土壤中黄曲霉的数量存在较大的差异,其中清远市土壤中的黄曲霉数量最多,达160 cfu/g干土壤,惠州和广州地区次之,均为100 cfu/g干土壤,汕头地区最少,在该地区抽取的土壤样品中未分离出黄曲霉菌株。在分离出来的黄曲霉菌株中,广州地区有10株产毒菌株、清远地区17株、惠州地区16株、云浮地区8株、河源地区8株。 相似文献