梨新鲜度与其挥发性成分的关系研究   总被引：1，自引：0，他引：1  

刘焕军  罗安伟  牛远洋  姚婕  李琛  李志成 《北方园艺》2016,(8):121-126

以砀山酥梨为试材,采用顶空固相微萃取-气相色谱-质谱联用(HS-SPME-GC-MS)技术,分析了砀山酥梨在(4±1)℃条件下挥发性成分的变化,检测了砀山酥梨可溶性固形物、硬度、酸度等新鲜度指标,探讨了砀山酥梨新鲜度与其挥发性成分之间的关系。结果表明:新鲜砀山酥梨在(4±1)℃条件下的保鲜时间为42d,其特征性气味成分主要为正己醇、1-辛醇;不新鲜砀山酥梨的特征性气味成分为2-甲基丁基乙酸酯、硅酸四乙酯、异戊醇、正己醇、1-辛醇、香叶基丙酮,且砀山酥梨由新鲜变为不新鲜时的阈值分别为1.57、2.74、1.39、15.15、211.13、2.36μg/L。试验结果为通过气味判断砀山酥梨的新鲜度提供了理论参考。  相似文献   

贵州魔芋软腐病菌多基因分子鉴定及其致病力分析     

黄露  周兵正  安星宇  王莉爽  姚令  陈小均  何海永  吴石平 《植物保护》2023,49(4):115-124

为明确贵州魔芋软腐病菌种类?致病力及分布特点, 采用组织分离法对贵州主要魔芋种植区软腐病样进行病原菌分离, 对icdA, mdh, mtlD, proA, rpoS 等5个管家基因进行了扩增?序列测定, 分别用单基因和多基因联合系统发育树对病菌进行鉴定, 同时采用组织块接种方法测定了不同菌株的致病力?通过组织分离法共分离魔芋软腐病菌株47株; 采用5个管家基因进行分子鉴定, 将病菌分别鉴定为海芋果胶杆菌Pectobacterium aroidearum?胡萝卜果胶杆菌Pectobacterium carotovorum和方中达迪基氏菌Dickeya fangzhongdai 3个种, 其中海芋果胶杆菌P.aroidearum为贵州魔芋软腐病主要致病菌, 占分离菌株的70%, 广泛分布在多个地区; 其次为方中达迪基氏菌D. fangzhongdai, 占分离菌株的28%, 也普遍存在于贵州各魔芋种植区; 胡萝卜果胶杆菌P. carotovorum最少, 占分离菌株的2%?致病力测定结果表明, 菌株间致病力存在一定的差异, 其中海芋果胶杆菌不同菌株之间致病力差异较大, 低?中?高致病力菌株都有, 方中达迪基氏菌差异较小, 仅有中?高致病力菌株?本研究确定了贵州魔芋软腐病菌种类?致病力及在贵州的分布特点, 首次报道了海芋果胶杆菌?方中达迪基氏菌是贵州魔芋软腐病的主要病原菌, 进一步加深了对魔芋软腐病及其发生流行的认识, 为软腐病的科学防控提供了科学依据?  相似文献   

不同基因型大麦苗期对干旱胁迫的响应及HvPIPs基因表达特征分析     

郝晶  姚立蓉  汪军成  司二静  杨轲  乔岩  孟亚雄  马小乐  李葆春  尚勋武  王化俊 《干旱地区农业研究》2023,(4):41-50

为研究不同基因型大麦苗期对干旱胁迫的响应,挖掘优质抗旱的大麦种质资源,以4份不同基因型大麦(2个高抗品种ZDM5430和ZDM5458,2个干旱敏感型品种7DCADA和IL-12)为材料,利用聚乙二醇(PEG-6000)模拟干旱条件,综合分析不同品种大麦的苗期抗旱性差异;并通过对不同品种根系进行PIPs实时定量分析,研究水通道蛋白AQPs基因与干旱胁迫之间的应答关系。结果表明,持续干旱胁迫会使大麦生长变缓,各处理苗长、根长、叶含水量和根含水量都呈下降趋势,其中,4个品种的大麦叶含水量在干旱胁迫14 d时下降最为显著,与对照相比,其降幅分别为29.14%、52.64%、21.67%和72.15%。干旱胁迫对大麦苗期干物质积累量和根冠比的影响较小,各处理间差异不显著;但持续干旱后,抗旱品种ZDM5430的根冠比呈上升趋势,在胁迫7、14、21 d时较CK分别增加了4.92%、42.86%和21.05%。随着胁迫时间的延长,大麦旗叶叶绿素含量和根系活力也呈下降趋势,其中ZDM5430和ZDM5458较对照组降幅较小,说明抗旱品种的形态指标、叶绿素含量及根系活力受干旱胁迫的影响较小。通过PIP...  相似文献   

低磷胁迫对红豆草光合、叶绿素荧光及内源激素的影响     

陈洁  南丽丽  汪堃  夏静  何海鹏  马彪  姚宇恒 《草地学报》2023,31(1):112-119

为探究低磷胁迫对红豆草(Onobrychis viciaefolia)光合作用、叶绿素荧光以及内源激素的影响,本试验采用营养液沙培法,以耐低磷5号和磷敏感1号红豆草为试验材料,设置常磷(1.00 mmol·L^-1 KH₂PO₄)和低磷(0.01 mmol·L^-1 KH₂PO₄)胁迫处理,于不同胁迫天数后测定形态、光合以及内源激素含量变化。结果表明：低磷胁迫抑制红豆草生长和光合作用,在低磷胁迫下,除非光化学淬灭系数外,供试红豆草叶绿素荧光参数均下降。随着胁迫时间的延长,2份红豆草材料地上和地下部玉米素(ZT)、脱落酸(ABA)、生长素(IAA)和赤霉素(GA₃)及其之间的比值变化趋势不一致,耐低磷红豆草地上和地下部ZT,GA₃,IAA,ABA耐低磷系数大于磷敏感材料。相关性分析表明,IAA与光合作用和叶绿素荧光显著相关,ABA与IAA,ZT,GA₃呈负相关。研究结果可为红豆草耐低磷特性研究提供...  相似文献   

陆地棉一个丝氨酸/苏氨酸激酶蛋白基因的克隆与表达分析   总被引：3，自引：1，他引：3  

杨郁文  倪万潮  张保龙  沈新莲  张香桂  徐英俊  姚姝 《棉花学报》2006,18(3):140-144

通过RT-PCR获得一个编码棉花丝氨酸/苏氨酸激酶类似蛋白全长的cDNA片段,其编码的氨基酸序列与拟南芥ATPK3的相似度达到82%,这个基因暂被命名为GhPK1。基因全编码区包括1038个核苷酸,编码346个氨基酸的蛋白。GhPK1的编码产物在265~270个氨基酸处有一个跨膜区并可能结合在内质网膜上。GhPK1在种子和纤维中的表达水平较其它组织高。另外,GhPK1的表达量在受到盐胁迫后上升,说明GhPK1可能参与了盐胁迫反应。  相似文献   

VISAR Data Reducing Method and Its Application to Impact Experiment     

YAO Guo-wen  LIU Zhan-fang 《保鲜与加工》2006,(8):25-28

Identification and adjustment on the disfigurements of original data of VISAR are probed by developing software for VISAR data reducing based on measure theory. Then the software is applied to plate impact experiment of ceramics. The disfigurements of light intensity data are identified and adjusted through Lissajou~ figures. The results indicate that VISAR data are reduced quickly and effectively by the software, and the exact free surface velocity history is obtained.  相似文献   

Research and Development of a Quality Control System in Construction     

GUO Ping  WU yuan hong  YAO Gang 《保鲜与加工》2002,(12):74

In the civil industry, quality is a key factor, so quality management and quality control play decisive roles in construction especially. At present, the main method of construction is quality controlling that engineers and technicians consult the standard and norm concerned, then check them one by one, and supervise the construction. It is not only inefficient but also easy to make mistake. Using computer technology in the quality control of construction will promote quality control level and improve management measures. Through the research of the quality control of construction,the system of quality control in construction is studied and developed, the structure of the software and its developed technology are expounded.  相似文献   

Electro-circuit Design About the Data Acquisition and Wireless Transmission System Based on High-speed SCM     

YAO Peng  XIE Zhi-jiang  YU Zhong-yun 《保鲜与加工》2006,(4):9-11

Considering the difficult of acquiring and transmission the signal from the moving equipment, a new fault diagnosis monitoring system is presented. The system acquires the state signal from the moving equipment by data acquisition module that based in W77E58 high-speed SCM, and transmits the data fast by wireless mode. The signal data can be use for fault diagnosis and precise analysis. The system has the ability for acquiring abundance of data with a high speed, the ability for high accuracy data conversion, and has a function of programme controlling gain and programme controlling low-pass filtering. The successful application of this system on the vibration monitor about the material-transporting bogie in an iron works of a domestic steel enterprise proves the stability and credibility about the design project, also testifies its practical value and versatility.  相似文献   

Effect of H2O2 in the Bioremediation of Petroleum Hydrocarbon Pollutant     

HUO Dan-qun  YAO Wei-jin 《保鲜与加工》2007,(10):130-133

The high efficient strain was separated and filtrated from the soil contaminated by petroleum hydrocarbon. The authors, we focus on the effect of H2 O2 in the bioremediation of petroleum hydrocarbon pollutant. The results show that the optimal concentration of H2O2was 200 mg/L at one time. At the lag phase of the strain, H2O2 was added in per eight hour, and at logarithmic phase, H2O2 was added in per two hour. Biodegradation rate of petroleum hydrocarbon was increased from 38. 1% to 83. 1%. The bioremediation of petroleum hydrocarbon pollutant is remarkably by double effects of deep oxidation and oxygen supply of H2O2.  相似文献   

The Reformation of the Construction Employment System and Building a Harmonious Society     

LIAO Yu - ping  YAO Bing  LIU Yi - sheng 《保鲜与加工》2006,(2):120-123

Construction industry is not only the pillar industry in national economy,but also the typical industry with intensive labors.As a major part of the construction industry,the Construction Employment System(CES) is an important factor of the market order,as well as the quality,the safety and the development of the industry.It is also an important part for building a harmonious society.It concerns the social harmony,political harmony,societal harmony,cultural harmony,urban-rural harmony,etc.However,at present,there are still many problems in CES that might influence the harmonious society building.This paper advises to speed up the reformation of CES,to establish and consummate the system of construction labor subcontract,to develop vigorously labor subcontract corporations,to improve the quality of the labors,to ensure the rights and interests of the labor.  相似文献