Analysis of gene expression profile of multidrug resistant MCF/DOX cell line after benflumetol derivative LY980503 treatment     

HUANG Feng  WAN Yong-ling  WU Da-long  LV Huan-zhang  GUO Jun-hua 《园艺学报》2003,19(8):1045-1048

AIM:To investigate the effect of LY980503(a benflumetol derivative)on multidrug resistance of tumor cell line using DNA microarray.METHODS:Total RNA was extracted from multidrug resistant MCF/DOX cell line. cDNA microarray containing 320 cDNAs was used to detect the gene expression profile.RESULTS:9 down-regulated genes and 1 up-regulated gene were identified after multidrug resistant MCF/DOX cells were treated with LY980503.CONCLUSION:LY980503 can effectively reverse the resistance of MCF/DOX to DOX in vitro by adjusting the expression of multi-genes.  相似文献   

Association of polymorphism of Fc receptor γ chain gene at position-29 in promoter with the susceptibility to systemic lupus erythematosus in southern Chinese     

ZHOU Shu-lu  YE Ren-gao  LIU Xiao-bo  ZHANG Hong  XU Han-shi  DU Yong  LI You-ji  YANG Nian-sheng  YANG Xiao  YU Xue-qing 《园艺学报》2003,19(6):782-785

AIM:To detect the association between the polymorphism of Fc receptor γ chain gene at position-29 in promoter and systemic lupus erythematosus(SLE).METHODS:The genotypes at position -29 in promoter of Fc receptor γ chain gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 180 patients with SLE and 140 ethnically matched controls in southern China.RESULTS:The frequencies of TT genotype(33.3%) and T allele (54.4%) at position -29 in patients with SLE were significantly higher than those in controls (17.2% and 42.9%, respectively), whereas, the frequencies of GG genotype (24.4%) and G allele (45.6%) in patients with SLE were remarkably lower than those in controls (31.4% and 57.1%, respectively) (P＜0.05). The TT genotype and T allele at position -29 were not associated with lupus nephritis in SLE patients (P＞0.05).CONCLUSION:Our results indicate that the T allele at position -29 in promoter of Fc receptor gene probably contributes to the susceptibility to SLE, but does not play a role in the occurrence of lupus nephritis.  相似文献   

Cloning of p15^INK4b related gene CCRG and expression analysis in the renal carcinoma     

JIN Gui-hua  YE Xiong-jun  CHANG Zhi-jie 《园艺学报》2003,19(10):1300-1304

AIM and METHODS:To analysis the factor that involved in renal carcinogenesis, we used the bait gene AK001518 to screen GenBank. To understand the relationship between cell cycle related gene(CCRG) and p15, we did RT-PCR and Northern Blot experiments. Then we examined CCRG expression level in renal carcinogenesis. RESULTS:Gained a function unknown gene CCRG that was 67% a mino acid identical with the gene AK001518 that was regulated by p15. It was shown that the CCRG mRNA was dramatically decreased when p15 gene was over-expressed. CCRG expression level was much higher in tumor tissues and cells than normal tissues and cells. CONCLUSION:The novel gene CCRG expressed highly in the renal carcinoma, which might play a significant role in the renal carcinogenesis.  相似文献   

Expression and role of CD134 and NF-κB in renal tissue of lupus nephritis     

ZHOU Yan-bin  YE Ren-gao  XIE Can-mao  XU Han-shi  GUAN Wei-ming  YANG Xiao  YANG Nian-sheng 《园艺学报》2003,19(9):1221-1223

AIM:To investigate the role of CD134 (OX40) and NF-κB in the pathogenesis of lupus nephritis (LN).METHODS:Renal in situ CD134 and NF-κB expression were examined in 40 biopsy specimens from LN patients by immunohistochemistry and microwave-based immunohistochemistry, respectively. The relationship between expression of CD134 and NF-κB was analyzed.RESULTS:The expression of glomerular and tubular CD134 and NF-κB in LN were higher than that in normal control, especially in class Ⅳ LN, where there was intense staining of endothelial cell, distal tubules, and interstitial mononuclear cell. The CD134 expression of glomerular and tubular was closely related to NF-κB expression, respectively (r=0.5542, P＜0.05;r=0.6279, P＜0.05). CONCLUSIONS:The abnormal expression of costimulatory molecule CD134 was well evidenced in LN. Strong expression of renal in situ NF-κB was likely mediated by CD134 signal pathway, which may play an important role in the pathogenesis of LN.  相似文献   

Effects of L-arginine on the production of extracellular matrix of human mesangial cells     

MA Kun-ling  LIU Bi-cheng  YE Yin-ying  LIU Nai-feng  RUAN Xiong-zhong 《园艺学报》2002,18(5):469-472

  相似文献   

Expression of undulin in experimental rat liver fibrosis     

YAO Ding-kang  LI Shi  KONG Xian-tao  YE Ting-jun 《园艺学报》2002,18(1):62-63

AIM: To observe the expression of undulin (Un) in liver tissue and to clarify the diagnostic significance of serum Un in experimental rat liver fibrosis. METHODS: Rat liver fibrosis was induced by carbon tetrachloride. The expression of Un in liver tissue was detected by immunohistochemical method. Serum Un levels was measured by enzyme immunoassay. RESULTS: Expression of Un increased in fibrotic liver than normal liver, and it was mainly distributed in portal tract stroma, central veins and fibrotic septa in fibrotic liver. Also, the level of serum Un was significantly higher in fibrotic liver than normal liver. CONCLUSION: These data suggest that Un should be a component of the hepatic extracellar matrix, and its expression could be increased greatly in fibrotic rat liver. Serum Un levels may be used as an indicator in liver fibrosis diagnosis.  相似文献   

优质牦牛肉肉质的综合评价   总被引：8，自引：0，他引：8  

冶成君 《青海畜牧兽医杂志》2004,34(4):18-19

2000-2002年对大通牛场40头6月龄不同性别的全哺乳牦牛的肉质进行了综合评价。对供试牦牛肌肉pH、嫩度、肌纤维细度、系水率、肉的贮存损失、肉的煮熟时间、熟肉率和烹饪损失率及肉色等进行了评价，分析了肌肉的化学成分，氨基酸及微量元素含量。结果表明该牦牛肉系水率强，肉的煮熟时间短，肉的贮存损失及烹饪损失较小，肌纤维较细，肉色深红。并具有蛋白质含量高，脂肪含量低及富含各种氨基酸的特点。  相似文献   

牛奶子锈病及其重寄生菌   总被引：3，自引：0，他引：3  

黄云  叶华智  董宝成  刘紫英  秦芸 《植物保护学报》2004,31(2):134-138

2002年在四川省宝兴县蜂桶寨国家自然保护区和天全县喇叭河自然保护区牛奶子上发现了牛奶子锈病,经鉴定,该锈病为牛奶子春孢锈菌Aecidiumelaeagni-umbellatae Diet.其病株率100%、病叶率26%,叶片枯斑、脱落和削弱树势.在牛奶子锈病菌上采集到自然重寄生菌,经鉴定为Tuberculina sp.,该重寄生菌为国内外首次发现.重寄生菌寄生于牛奶子锈菌春孢子器出口处,阻碍春孢子的释放,具有潜在的生防应用价值.笔者对牛奶子锈病的症状特点、病原形态特征及牛奶子锈病菌的重寄生现象与重寄生菌的形态特征等进行了研究.  相似文献   

甘肃河东春季透雨日期与海温的相关分析   总被引：9，自引：1，他引：9  

郭江勇  叶燕华  林纾  马力文 《干旱地区农业研究》2004,22(1):18-22

利用甘肃河东和宁夏回族自治区中南部1971～2000年67个站的春季第一场透雨日期资料,用EOF和REOF方法对其做了分解,进行了透雨日期的气候分区,分析了春季第一场透雨日期的时空分布特征,并计算了其与北太平洋海温的相关关系。结果表明,北部透雨平均日期与南部透雨平均日期具有相反的趋势,北部最迟在5月下旬,南部最早在4月中旬;20世纪70年代透雨日期偏迟,80年代透雨日期偏早,90年代偏迟。透雨早的年份,春季降水偏多,透雨迟的年份,春季降水偏少;透雨日期与北太平洋海温呈显著的负相关,当北太平洋地区的海温偏高时,甘肃河东春季透雨日期偏早。当北太平洋地区的海温偏低时,甘肃河东春季透雨日期偏迟。  相似文献   

天水小陇山锐齿栎群落土壤全N分布特征及与生物多样性关系研究   总被引：2，自引：0，他引：2  

张俊华  王胜利  巨天珍  南忠仁 《干旱区资源与环境》2004,(Z2)

对天水小陇山锐齿栎群落不同演替阶段土壤全N含量进行了分析 ,探讨了土壤全N与群落生物多样性之间的关系 ,结果表明 :随发育阶段土壤全N含量呈稳定的变化趋势。发育中期 ,随多样性指数、均匀度指数、优势度指数的增加 ,全N含量呈“V”型变化趋势 ,随丰富度指数增加 ,全N含量呈逐渐减小趋势 ;发育后期 ,随着多样性指数、丰富度指数、优势度指数的增加 ,全N呈逐渐增加趋势 ,随着均匀度的增加 ,全N变化不明显 ;发育末期 ,多样性指数、均匀度指数、优势度指数趋于稳定 ,土壤全N含量不稳定 ,随丰富度指数的升高 ,全N有先升高后降低的趋势。  相似文献