用分子生物学方法鉴别检测动物源性饲料中的牛羊源性成分   总被引：10，自引：0，他引：10  

杨宝华  宗卉  林庆燕  俞火 《中国畜牧杂志》2002,38(1):3-5

为了防止海绵状脑病疫区和痒病疫区反刍动物源性饲料进入我国 ,非常有必要对贸易往来中的肉骨粉品种来源进行鉴定检测。我们采用分子生物学方法从动物源性饲料中鉴别检测牛、羊特异性线粒体DNA的片段 ,并用限制性内切酶SspⅠ对PCR产物进行酶切鉴定及DNA序列测定。该方法的灵敏度可达 0 .12 5 % ,具有灵敏度高、快速和特异性强的优点。  相似文献   

Effect of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponense     

Wei-Na  Wang An-Li  Wang Yue  Wang Junxia  Wang Ru-Yong  Sun 《Journal of the World Aquaculture Society》2005,36(1):1-7

This study examined the effects of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponese . The response of the cellular defense was determined by monitoring changes in reactive oxygen intermediates in hemocytes, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione reductase and glutathione transferase activity in muscle. The results showed that prawns fed the basal diet had a significant increase in reactive oxygen intermediates in response to exposure to ammonia-N. When dietary vitamin C was added to the feed, there was a significant decrease in reactive oxygen intermediates in response to exposure to ammonia-N. Total superoxide dismutase and glutathione peroxidase activity of prawns fed the basal diet decreased as ammonia-N increased. This decrease in activity was significantly less pronounced in prawns that were fed the dietary vitamin C supplement. In prawns that were fed the dietary vitamin C supplement there was no significant variation in catalase reductase or gluxathione reductase activity in response to variations in ammonia-N concentration. The glutathione transference activity increased at a low concentration of ammonia-N in prawns that were fed the dietary vitamin C supplement, but there was no significant variation in the prawns fed the basal diet. Overall the results indicate that dietary vitamin C supplements enhance the capacity of M. nipponese to respond to elevations in ammonia-N. This response involved several biochemical reactions known to be involved in cellular defense.  相似文献   

GRC构件在建筑装饰业的应用     

朱文波  崔月 《林业科技情报》2001,33(4):21-21

本文系统地阐述了GRC构件的产品性能,施工工艺及方法、特点,展望未来建筑业外装饰的发展趋势。  相似文献   

鸭大肠杆菌病流行病学与病原特性研究     

鲍国连  韦强  季权安  佟承刚  俞坚群  盛楚江 《浙江农业学报》2001,13(3):147-151

对浙江等地鸭大肠杆菌病流行病学进行调查，并研究其发病特点，分离鉴定出致病性大肠杆菌26株；已对其中20株菌株进行血清型鉴定，主要为078，占70%（14/20），其次是O1，占15%（3/20）；人工感染试验表明，80%（8/10）菌株毒力强，皮下、肌肉、足蹼3种途径均易复制该病；病理组织学检查肝、肺、脾等，表现急性炎症，出血等病变；药敏试验表明，不同菌株对药物敏感性差异较大，除对鸭菌消、卡那霉素等高敏外，对多数常用抗菌药已耐药，最后就该病的防治提出了对策。  相似文献   

浅谈林地资源保护管理与生态林业建设   总被引：4，自引：0，他引：4  

陈日红  余峰 《浙江林业科技》2001,21(4):68-70

论述了建德市林地资源保护和生态林业建设现状以及他们间的相互关系，并提出要搞好生态林业建设必须建立林地资源保护管理体系，对林地资源要实行总体规划，合理使用。  相似文献   

蓝芩注射液体外抑菌试验   总被引：1，自引：0，他引：1  

李蓉  严宏凤  于生兰 《中兽医医药杂志》2006,25(6):42-42

大肠杆菌、猪霍乱沙门氏菌、葡萄球菌、猪链球菌是常见病原菌，在不同诱因存在下引起发病，对这类疾病多数采用抗菌药物治疗，细菌易产生耐药性。耐药性的产生又需要加大药量或变换药物品种，这样既增加了治疗的成本，又使得畜产品中的药物残留问题更加突出。许多中药具有抗菌消炎和调节机体免疫的功能，并具有副作用小、残留低、不易产生耐药性等优点。笔者等观察了蓝芩注射液对4种病原微生物的体外抑菌作用，现报道如下。  相似文献   

实施天保工程 促进生态良性循环     

于杰  罗继生  崔久霞 《防护林科技》2006,(3):86-86

鹤岗市林业局红旗林场建于1956年，位于小兴安岭的边陲，施业面积18952hm^2，由于近几年来可采资源减小，经济发展缓慢，企业职工收入低，生活困难，森林资源过度消耗，企业出现经济危机状态，当务之急，我们按照黑龙江省林业厅统一部署，在2001年实施了“天保工程”，森林覆盖率由原来的78．0％增加到现在的81．4％，蓄积量由原来的62万m^3增加到现在的74万m^3，特别是近3a对天然林全面实行封采，取得了显著效果。  相似文献   

通过农杆菌介导法将口蹄疫结构蛋白全长基因P1导入大豆的研究     

王萍  郭永来  王罡  季静  余云舟  李昌  金宁一 《西南农业学报》2005,18(5):643-646

本研究以大豆体细胞胚为受体。用农杆菌介导法将口蹄疫病毒结构蛋白全长基因P1导入大豆基因组中．获得了抗性植株。经GUS染色、PCR及PCR-southern杂交等分子检测。证明目的基因已导入并整合到大豆基因组中。为利用大豆作为生物反应器生产口蹄疫新型口服疫苗奠定了基础。  相似文献   

Identification of Heat Tolerance Linked Molecular Markers of Chinese Cabbage (Brassica campestris L.ssp. pekinensis)   总被引：1，自引：0，他引：1  

ZHENG Xiao-ying  WANG Yong-jian  SONG Shun-hua  LI Li  YU Shuan-cang 《中国农业科学(英文版)》2002,1(7):786-791

Genetically stable population of recombination inbred line (RIL) was derived from a cross between a heat tolerant line 177 and a heat sensitive line 276 of Chinese cabbage (Brassica campestris L. ssp.pekinensis ) by single seed descent. The RILs were analyzed using isozyme, RAPD and AFLP techniques in order to find molecular markers that are linked to heat tolerance quantitative trait loci (QTL). The results of variance analysis of single factor indicated that there were 9 molecular markers closely linked with heat tolerance QTL, including 5 AFLP markers, 3 RAPD markers and 1 PGM isozyme marker. Total genetic contribution of these makers to heat tolerance was 46.7%. Five of the nine markers distributed in one linkage group,the remaining 4 markers were located in separate groups. Thus the 9 heat tolerance linked markers distributed in 5 independent locations in the genome of Chinese cabbage.  相似文献   

通用开放式绘图仪接口软件设计与实现     

王跃  王东 《湖南农业大学学报(自然科学版)》1993,20(3)

本文针对TANGO软件所支持的图形输出设备中不包括CALCOMP系列绘图仪的问题,设计了TANGO图形文件与CALCOMP系列绘图的接口软件.由此阐述了一种通用图形描述语言及开放式绘图仪接口软件的设计思想与技术实现.  相似文献