Influence of paricalcitol on renal tubulointerstitial fibrosis in diabetic nephropathy     

WANG Lai-liang  LUO Qun  CAI Ke-dan  ZHOU Fang-fang  GAO Yan-hong 《园艺学报》2015,31(4):719-724

AIM: To investigate the effect of paricalcitol (P) on renal tubulointerstitial fibrosis and the underlying mechanisms in diabetic nephropathy (DN).METHODS: DN rat model was induced by a single intraperitoneal injection of streptozotocin after fasting. The animals were randomly divided into 2 groups:the DN rats in paricalcitol-intervened group (group P) were injected intraperitoneally with paricalcitol dissolved in propylene glycol after the day when the model was induced successfully at a dose of 0.4 μg/kg (3 times a week); the DN rats in DN group (group D) were given isopyknic propylene glycol. Normal control group (group C) was also set up. The samples of blood, urine and renal tissue were collected after intervention of paricalcitol for 12 weeks. The biochemical indexes were measured. The renal tissues were used for pathologic observation and determining the expression of transforming growth factor-β1 (TGF-β1), Wnt-4, β-catenin and Klotho by immunohistochemistry and Western blotting. In addition, the correlation among the above indexes was analyzed.RESULTS: (1) Scr, BUN and 24 h urine protein increased significantly in group D compared with group C, while decreased in group P compared with group D (P<0.05). (2) The area of renal tubulointerstitial fibrosis increased in group D compared with group C, while decreased in group P compared with group D (P<0.05). (3) The expression of Klotho decreased, while the expression of TGF-β1, Wnt-4 and β-catenin increased in group D compared with group C (P<0.05). Compared with group D, the expression of Klotho increased, while the expression of TGF-β1, Wnt-4 and β-catenin decreased in group P (P<0.05). (4) The expression of Klotho was negatively correlated with the fibrosis area, TGF-β1, Wnt-4 and β-catenin (P<0.05).CONCLUSION: Paricalcitol inhibits renal tubulointerstitial fibrosis in DN by promoting the expression of renal Klotho, and inhibiting Wnt/β-catenin signaling pathway activation and TGF-β1 synthesis.  相似文献   

贞丰县银洞湾小流域的石漠化治理模式     

周仕林 《林业勘查设计》2015,(1):72-74

对贵州省贞丰县银洞湾小流域石漠化的治理对策及模式进行了介绍。  相似文献   

Comparative Study on Carcass Traits and Meat Quality of Different Month Old of Xinjiang Brown Cattle Steers     

YAN Xiang-min  ZHANG Jin-shan  LI Hong-bo  LI Na  DU Wei  ZHOU Zhen-yong  ZHANG Yang 《中国畜牧兽医》2015,42(11):2954-2960

In this research,12 heads of 28 to 30 months (low months age group) and 8 heads of 32 to 34 months (high months age group) Xinjiang Brown cattle steers which fed in the same mode of nutrition were chose for the slaughter test,in which the carcass traits,nutrient composition and some edible quality index were measured and comparative analyzed,a research which through comparative analysis the carcass traits and meat quality of nutritional components of different months of Xinjiang Brown cattle steers was carried out.The results indicated that the marbling,carcass grade,fat,meat color of high months age group were better than that of low months age group,the traits of the low months age group on fat color,moisture,protein,cooking loss and shear force value were better than that of high months age group,and there were significant difference on the traits of fat and meat color L^* between the two groups (P<0.05),while the differences of other traits between them were not significant (P>0.05);As high-grade beef,in addition to the traits of meat color and pH value of the chunk roll,tenderloin and striploin,each site of the meat traits on the fat,protein,moisture,cooking loss,shear force value indicators were all better than other parts,and showed the best of nutritional value and edible quality.28 to 34 months of Xinjiang Brown cattle steers could produce high-grade beef,the meat could be used as the raw materials of steak and boiled beef.  相似文献   

海口地区平原型热带高尔夫园林造景技法探析     

王影  雷金睿  何荣晓  周鹏 《热带生物学报》2015,6(1):78-86

采用实地调查与文献研究相结合、定量分析与定性分析相结合的方法,对海口地区3个平原型热带高尔夫园林的空间布局、植物造景方法、景观元素搭配、人工植物群落进行了调查分析。结果表明:3个球场的空间布局和植物造景都略显单调,隔景和列植分别为其最主要的造景方式;景观元素的搭配形式则较丰富,热带植物及色叶植物的运用使其别具风格和魅力;人工植物群落方面则多以常见园林植物和外来入侵物种为主,缺乏乡土植物的运用和搭配,人工痕迹明显。  相似文献   

点杂交分析小鼠CRBN基因组织表达     

唐文惠  周蓉  陈佩林 《湖北农业科学》2011,50(4):857-859

  相似文献   

卷烟感官评价三点检验Excel插件的设计和应用     

赵维一  温若愚  曾建  周学政 《湖北农业科学》2011,(17):3616-3618

为提高卷烟感官评价三点检验的易用性和实用性,采用Excel VBA设计三点检验Excel插件程序,随机生成样品编码,快速识别评价样品,自动统计分析结果,实现了卷烟感官的高效评价。  相似文献   

单胃动物脂肪营养研究进展     

张婷  钟伟  周宁 《中国畜牧兽医》2014,41(3):142-146

Fat is an essential element for animal.The paper reviewed recent research progress on fat nutrition in monogastric animal at home and abroad, combining with the fat digestion and absorbing form in animal body, and summarized biological function and application of fat in production, so as to provide theoretical basis for better utilization of fat in animal production and exploration its mechanism of action.  相似文献   

三种羊蹄甲属植物物候特性研究     

周玉洁  闫湘  何妙坤  王智源  黄久香 《湖南林业科技》2017,44(3)

物候特性研究能够深入了解植物的生长特性,为植物栽培养护、分类、杂交育种与园林应用提供参考依据。通过定期定点观测记录的方法,研究了南方地区常用的三种羊蹄甲属观花乔木宫粉紫荆、羊蹄甲与红花羊蹄甲的物候特性,结果表明:(1)三种羊蹄甲属植物的新梢生长均集中于夏季,但持续时间以及生长高峰期稍有差异,红花羊蹄甲与羊蹄甲生长趋势较为一致,而宫粉紫荆受高温影响较为明显,高温期间生长减缓。(2)三种羊蹄甲的花期不同,宫粉紫荆的花期为12-翌年4月,羊蹄甲花期为10-12月,红花羊蹄甲花期最长,为11-翌年5月,三种羊蹄甲属植物的花色、花序、单花花期等稍有不同。(3)综合三种羊蹄甲属植物的新梢生长与开花物候特性,表明红花羊蹄甲与羊蹄甲的亲缘关系更为接近。  相似文献   

西藏地区不同作物秸秆体外发酵特性研究     

巴桑珠扎  陈亮  奥斯曼  于翠翠  赵丽  周传社  李斌 《动物营养学报》2017,29(2)

本试验旨在利用体外产气法评定西藏地区不同作物秸秆的营养价值。选取西藏地区5种常见作物秸秆(豌豆秸秆、玉米秸秆、青稞秸秆、小麦秸秆、油菜秸秆),通过单因素试验设计,利用体外产气法评定5种作物秸秆体外发酵48 h的产气量、理论最大产气量(Vf)、甲烷(CH4)产量、逻辑斯谛-指数(LE)模型产气参数、发酵液p H和氨态氮(NH3-N)浓度、主要挥发性脂肪酸(VFA)产量、体外干物质降解率(IVDMD)和体外中性洗涤纤维降解率(IVNDFD)等指标。结果显示:5种作物秸秆体外发酵48 h的产气量按玉米秸秆、青稞秸秆、豌豆秸秆、小麦秸秆、油菜秸秆的顺序依次降低。体外发酵48 h后,玉米秸秆的Vf、IVDMD、IVNDFD、CH4产量(豌豆秸秆除外)、丙酸和总VFA产量均显著高于其他4种作物秸秆(P0.05),其体外发酵液p H则显著低于其他秸秆(P0.05)。结果表明,玉米秸秆体外发酵效果最佳,与其他试验作物秸秆相比更容易被瘤胃微生物降解利用。  相似文献   

不同规格穴盘育苗对茄苗及其栽培效益的影响     

周洪  朱鹏飞  徐佳 《蔬菜》2017,(10):11-13

为完善茄子穴盘育苗技术,对比研究了不同规格穴盘育苗的茄苗素质及栽培效益。结果表明:32孔规格穴盘育苗比50孔茄苗素质明显提升;移栽单栋钢架大棚后,32孔穴盘育苗的茄子早熟性比50孔穴盘育苗平均提前5 d,总产量增加15.3%;667 m~2投入成本32孔穴盘育苗比50孔穴盘育苗增加75.3%(35.6元),而收益增加了2 427.9元。因此建议大棚茄子种植采用32孔规格穴盘进行育苗。  相似文献