全文获取类型
收费全文 | 46748篇 |
免费 | 2792篇 |
国内免费 | 4588篇 |
专业分类
林业 | 3577篇 |
农学 | 3085篇 |
基础科学 | 2502篇 |
4877篇 | |
综合类 | 22068篇 |
农作物 | 3376篇 |
水产渔业 | 2107篇 |
畜牧兽医 | 7143篇 |
园艺 | 3342篇 |
植物保护 | 2051篇 |
出版年
2024年 | 288篇 |
2023年 | 879篇 |
2022年 | 2121篇 |
2021年 | 2131篇 |
2020年 | 2027篇 |
2019年 | 1893篇 |
2018年 | 1368篇 |
2017年 | 2170篇 |
2016年 | 1525篇 |
2015年 | 2350篇 |
2014年 | 2417篇 |
2013年 | 2910篇 |
2012年 | 3963篇 |
2011年 | 4063篇 |
2010年 | 3713篇 |
2009年 | 3412篇 |
2008年 | 3421篇 |
2007年 | 3199篇 |
2006年 | 2562篇 |
2005年 | 1960篇 |
2004年 | 1339篇 |
2003年 | 855篇 |
2002年 | 897篇 |
2001年 | 858篇 |
2000年 | 716篇 |
1999年 | 329篇 |
1998年 | 112篇 |
1997年 | 106篇 |
1996年 | 81篇 |
1995年 | 73篇 |
1994年 | 63篇 |
1993年 | 65篇 |
1992年 | 63篇 |
1991年 | 34篇 |
1990年 | 31篇 |
1989年 | 26篇 |
1988年 | 13篇 |
1987年 | 21篇 |
1986年 | 12篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1982年 | 3篇 |
1981年 | 6篇 |
1970年 | 1篇 |
1966年 | 2篇 |
1964年 | 1篇 |
1962年 | 9篇 |
1958年 | 1篇 |
1956年 | 23篇 |
1955年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
82.
83.
哺乳犊牛的消化特点与蛋白质需要 总被引:7,自引:0,他引:7
本文从犊牛的消化生理特点出发,综述了犊牛出生后的生理特征及蛋白质、必需氨基酸的需要量,并对代乳品中蛋白质原料进行了论述。 相似文献
84.
试验将槐花蜂花粉进行振动磨超微粉碎破壁,分别按1 g/kg、5 g/kg、10 g/kg三个不同剂量混到蛋鸡饲料中,从7日龄开始饲喂至50日龄,每个剂量分两个试验,试验一用新城疫Ⅳ系疫苗免疫雏鸡,利用β-微量法监测血清中的血凝抑制抗体效价的动态变化;试验二用大肠杆菌油乳苗免疫雏鸡,利用间接血凝法监测血清中大肠杆菌抗体效价的动态变化。分别定期称重捕杀取脾、胸腺、法氏囊,计算免疫器官指数,取肠道内容物计数大肠杆菌和乳酸菌,测定肠道内pH值。结果表明,不同剂量的破壁蜂花粉均能不同程度地提高病毒抗原性抗体效价和菌体抗原性抗体效价,对雏鸡免疫器官的发育以及肠道内环境、菌群的平衡也有一定影响。 相似文献
85.
86.
XU Rui ZHANG Yun ZHANG Mei GE Zhi-ming CHEN Wen-qiang LI Xiu-chang CAI Heng ZHU Hui 《园艺学报》2003,19(5):642-644
AIM:To elucidate the relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats (SHR).METHODS:Intracellular free calcium concentrations were measured by Fura 2 methodology and left ventricular function quantitated by cardiac catheterization in 20 SHR aged 10, 22, and 34 weeks and 20 age-matched Wistar-kyoto (WKY) rats.RESULTS:(1) The systolic blood pressure(SBP), intracellular calcium concentrations and left ventricular mass / body weight index (LVM/BW) were significantly higher in all three age groups of SHR than the corresponding groups of WKY; (2) Compared with age-matched WKY groups, the peak left ventricular pressure descending rate(-dp/dtmax) decreased while left ventricular relaxation time constant (τ)increased significantly in SHR aged 22 and 34 weeks. The peak left ventricular pressure ascending rate(dp/dtmax) and the left ventricular contractility index were significantly increased only in the 34 weeks SHR; (3) Intracellular calcium concentrations showed a positive correlation with LVM/BW,SBP,-dp/dtmax and τ(r=0.47-0.83,P<0.01)and a negative correlation with dp/dtmax and the left ventricular contractility index (r=-0.46,P<0.05 and r=-0.81, P<0.01).CONCLUSION:Intracellular calcium overload is one of the potential mechanisms in the induction of left ventricular hypertrophy as well as of systolic and diastolic dysfunction. 相似文献
87.
AIM:To explore the effect of brain ischemia injury on cell proliferation and nestin expression in cortex and subependymal zone (SEZ).METHODS:Using a local brain ischemia model(MCAO), BrdU positive cells of cortex and subependymal zone (SEZ), also nestin positive cells, were observed by immunohistochemistry.RESULTS:BrdU and nestin positive cells in SEZ of MCAO rats were obviously increased. In cortex, only nestin positive cells were observed.CONCLUSION:Neural stem cells in SEZ and cortex were activated after brain ischemia, it may be related with neural recovery after brain ischemia injury. 相似文献
88.
AIM: To investigate the protective effect of onychin on the endothelial cells injured by oxidative stress. METHODS: The injured model was established by endothelial cells treated with menadione. The growth inhibitory rate of endothelial cell was determined by MTT assay; NO2-/NO3- concentration in the medium was determined by nitrate reductase assay; eNOS and caveolin-1 protein levels were determined by Western blot. RESULTS: Onychin significantly decreased the growth inhibitory rate of endothelial cells injured by menadione, increased NO2-/NO3- concentration in the medium and eNOS activity and up-regulated caveolin-1 expression. CONCLUSION: Onychin possesses a protective effect against endothelial cell injury induced by menadione via caveolin-1/eNOS pathway. 相似文献
89.
AIM:To manufacture recombinant protein of the highly conserved domain in human bone morphogenetic protein-1(BMP-1) using gene engineering methods as antigen for making wide spectrum antibody to BMP-1.METHODS:We analyzed the gene sequences and protein structures of BMP-1 and its related proteins, and chose a highly conserved fragment as target gene. Total RNA was prepared from human osteosarcoma cell line Saos-2, then the target gene was amplified with RT-PCR. The PCR product was cloned into prokaryotic expression vector pMAL c2 to get recombinant vector BMP-1(322-588aa)-pMAL c2. After transforming the recombinant plasmid into DH5-alpha and screening, several prositive clones were got for sequencing. Finally the transformed cells was induced with IPTG to get fusion protein.RESULTS:The BMP-1 gene fragment was successfully cloned into vector pMAL c2, and was able to express efficiently with IPTG inducement. The amount of expressed fusion protein is about 66%-72% in total volume of bacterial proteins.CONCLUSIONS:The recombinant protein contains several key domains(2 CUB domains and 1 EGF domain), which are shared by BMP-1 and its related proteins. Specific wide spectrum antibody to human BMP-1 and its related proteins may be generated with this recombinant protein antigen. 相似文献
90.
LEI Jun-xia LI Hao-wei HUANG Chun-nong ZHU Mei-ling WEN Guan-mei ZHANG Xiu-ming LI Yan LI Shu-nong 《园艺学报》2003,19(10):1325-1330
AIM:To investigate multi-potential of rat bone marrow mesenchymal stem cells (rBMMSC) and mutation inclination, the rBMMSC were long passaged in vitro. METHODS:Cellular cycles of different passages were assayed by FACSan flow cytometry and karyotypes of passage 6, passage 25 and passage 45 were compared by G-binding analysis. RESULTS:The early passages and long-term passages all showed strong proliferation; passage 6, passage 25 and passage 45 all showed normal karyotype. CONCLUSION:Long-term culture and passage of rBMMSC still remains strong proliferation. With this capability, the mutation inclination is not enhanced. 相似文献