柔性机械式大豆精量排种器设计与试验     

侯守印  邹震  魏志鹏  祝祎璠  陈海涛 《农业机械学报》2020,51(10):77-86,108

针对免耕播种作业时沙土和碎秸等飘浮物被气吸式排种器吸入充种室内,导致排种工作部件损坏、排种质量降低等问题,设计了一种柔性清护种机械式大豆精量排种器。对排种器工作过程进行理论分析,确定了影响工作性能的主要结构与工作参数;通过单因素对比试验确定了对排种性能影响最小的护种毛刷材料;采用三因素五水平二次正交旋转中心组合试验方法,以作业速度、充种倾角、护种距离为试验因素,以合格指数、漏播指数、重播指数和破损指数为评价指标,应用Design-Expert 8.0.6.1软件对试验结果进行方差分析。结果表明：各因素对合格指数的影响显著性由大到小依次为作业速度、护种距离、充种倾角,各因素对漏播指数的影响显著性由大到小依次为护种距离、作业速度、充种倾角,各因素对重播指数的影响显著性由大到小依次为作业速度、充种倾角,护种距离影响不显著,各因素对破损指数的影响显著性由大到小依次为护种距离、作业速度、充种倾角;当作业速度为8~12km/h、充种倾角70°、护种距离为-1.5mm时,排种合格指数大于94%、漏播指数小于3%、重播指数小于3%、破损指数小于0.2%,满足免耕大豆高速精量播种作业要求。  相似文献   

Overexpression of SSTR2 inhibits growth of SSTR2-positive tumors via multiple signaling pathways     

XIAO Xiao-ping  LI Yue-qin  XU Bin  LI Hong-jian  ZHANG Xin  ZHOU Tian-hong  ZOU Yi 《园艺学报》2009,25(7):1254-1261

AIM: To study the anti-proliferation effect of overexpressed SSTR2 in experimental cancer with different profiles of endogenous SSTRs expressions and the possible signaling pathways involved. METHODS: In the first experiment, the growth of the tumor xenografts of the inoculated capan-2 cells and A549 cells overexpressing SSTR2 or LacZ was investigated in nude mice. In the second experiment, the adenoviral vector expressing SSTR2 were introduced into experimental capan-2 xenografts by intratumoral injection. The growth inhibition of these experiment tumors was observed and the potential influences on different signaling pathways were analyzed by immunoassays. RESULTS: Overexpression of SSTR2 inhibited the growth of tumors with different profiles of endogenous SSTRs, including experimental capan-2 xenografts that had endogenous SSTR2 expression. Overexpression significantly affected a number of components in apoptotic pathway, MAPK pathway and angiogenesis. CONCLUSION: SSTR2 is a promising candidate for gene therapy in a wide spectrum of cancers.  相似文献   

Jagged1 knocking down in endothelial cells accelerates PDGF induced proliferation and migration of vascular smooth muscle cells in rats     

WU Xiao-jing  HUANG Lan  ZHOU Qi  CHEN Jian-fei  ZHOU Yin-pin  NIU Qin  ZOU Yun-zeng  GE Jun-bo 《园艺学报》2009,25(10):1873-1877

AIM: To investigate the effect of Jagged1 expression in endothelial cells (EC) on platelet derived growth factor (PDGF) induced proliferation and migration of vascular smooth muscle cells (VSMC) in rat.METHODS: Rat aorta EC was inoculated in the lower chamber and VSMC were in the upper chamber of the cell coculture system. Three groups were divided: control, sicontrol and siJagged1. The EC Jagged1 protein expression was assayed by Western blotting to evaluate small RNA interfering (RNAi) efficiency. After the cells were cocultured with PDGF for 24 h, the proliferation and migration of VSMC were respectively evaluated by ［3H］-TdR incorporation and migrating cells counting. Protein expression of α-SM-actin in VSMC was assayed by Western blotting. RESULTS: The Jagged1 protein expression in EC was significantly lower in siJagged1 group than that in control group (0.26±0.02 vs 0.67±0.02, P<0.05), and no statistic significance was observed between control and sicontrol groups. The VSMC ［3H］-TdR incorporation and migration were higher in PDGF +siJagged1 group than those in PDGF group {［3H］-TdR incorporation (23 074±2 702) counts·min-1·well-1 vs (16 442±1 803)counts·min-1·well-1, n=5, P<0.05; migration (27±4) cells/field vs (15±3)cells/field, n=5, P<0.05}. The α-SM-actin protein in VSMC was lower in PDGF + siJagged1 group than that in PDGF group (0.25±0.06 vs 0.49±0.04, n=3, P<0.05).CONCLUSION: Jagged1 knock down in rat EC accelerates PDGF induced proliferation and migration of VSMC. These results suggest that Jagged1 expression in EC plays an important role in maintaining VSMC contract phenotype and inhibiting VSMC overgrowth after arterial injury.  相似文献   

番茄晚疫病抗病育种及分子生物学研究进展   总被引：2，自引：1，他引：1  

邱夷鹏  张子君  李海涛  邹庆道 《中国蔬菜》2009,1(10):1-6

对国内外近年来在番茄晚疫病菌的病原学、抗病资源的筛选鉴定、抗病机制的研究及分子生物学方面取得的进展进行了综述,并展望了今后的研究重点和方向。  相似文献   

不同基质栽培对番茄叶片光合作用和根系ATP酶的影响   总被引：1，自引：0，他引：1  

陈双臣  程伟霞  刘爱荣  贺超兴  邹志荣 《中国蔬菜》2009,1(14):23-27

以腐熟玉米秸、麦秸、菇渣、锯末等农产废弃物添加土壤后配成有机基质进行番茄栽培试验,探讨不同基质栽培对番茄叶片光合特性和根系ATP酶活性的影响。结果表明,有机基质栽培较土壤栽培植株同化产物积累增加,番茄光合速率、细胞间隙CO₂浓度、呼吸速率均显著提高。有机基质栽培Fv/Fm、ΦPSⅡ、qP显著升高,P-H⁺+ATPase、P-Ca²⁺-ATPase、V-H⁺-ATPase、V-Ca²⁺-ATPase活性提高。有机基质栽培番茄通过提高根系质膜和液泡膜ATPase活性、增加番茄的光合速率、细胞间隙CO₂浓度,提高Fv/Fm、ΦPSⅡ、qP,提高光化学效率,增强营养吸收,从而促进番茄生长和同化物形成。  相似文献   

西瓜EST-SSR分子标记的筛选   总被引：1，自引：0，他引：1  

邹明学  陈艳红  徐勇  郭绍贵  张海英  宫国义 《长江蔬菜》2009,(18):13-14

利用国际共享获得的820条EST序列,自主设计出79对EST-SSR引物,其中有32对引物经过PCR扩增出稳定清晰的电泳条带,可以作为西瓜和相关物种的新的EST-SSR分子标记。  相似文献   

Effect of membrane environment at high temperature on delayed rectifier K⁺ channel in hypothalamus neurons     

CAI Chun-qing  ZOU Fei 《园艺学报》2000,16(2):105-108

AIM: To observe the effect of high temperature on the delayed rectifier K⁺ channel(I_K) in hypothalamus neurons METHODS: The kinetic properties of I K were measured by cell-attached mode of patche-clamp technique RESULTS: The conductance, long-term open constant(τ _o2) and open probability(Po) of I_K increased with increase of temperature (P <0.05), while more burst open and multi-order open were recorded Many types of currents occurred after heat stress at 43℃,and voltage-dependent property reduced and even depressed CONCLUSIONS: The activating action of I_K might be affected obviously by high temperature, the coupling between I K and body fever or heatstroke remained to be established.  相似文献   

甘蔗叶不同还田方式对土壤养分的影响   总被引：1，自引：0，他引：1  

文少白  葛畅  李明  邹雨坤  侯宪文 《热带作物学报》2017,(8):1423-1426

为减轻秸秆焚烧所带来的环境问题和充分利用甘蔗叶资源,在大田环境下动态监测甘蔗叶粉碎、焚烧和深埋3种还田方式对土壤养分的影响。结果表明:甘蔗叶粉碎还田和深埋还田有利于甘蔗叶中有机碳及氮、磷、钾养分的缓慢释放;甘蔗叶焚烧还田提高了土壤碱解氮含量5.6~20.9 mg/kg和速效磷含量0.8~4.0 mg/kg,促进土壤中氮磷向有效态转化,焚烧还田初期显著增加了土壤速效钾的含量12.3~18.3 mg/kg,但焚烧会损失部分甘蔗叶中碳氮元素,也造成环境污染。综合分析认为甘蔗叶粉碎还田是比较省工、方便的处理方式。  相似文献   

不同氮肥水平下蚯蚓粪施用对水稻生长特性及产量的影响     

赵春容  单双吕  王玉梅  罗凯  周雪峰  黄敏  张恒栋  范龙  曹放波  陈佳娜  方升亮  邹应斌 《中国稻米》2017,(4):67-70

以两优培九为材料,采用大田微区试验,研究不同氮肥水平(N1,9 g/m~2;N2,12 g/m~2;N3,15 g/m~2)下不同蚯蚓粪施用量(EC0,0;EC_1,16.88 kg/m~2;EC_2,33.76 kg/m~2)对水稻生长及产量形成的影响。结果表明,移栽40 d后EC_2和EC_1处理的茎蘖数均显著高于EC0(P0.05);幼穗分化期和乳熟期EC0处理的SPAD值均显著低于EC_1和EC_2处理(P0.05);齐穗期EC0处理的叶面积指数分别比EC_1和EC_2处理低32.7%和80.7%,地上部总干物质量分别比EC_1和EC_2处理低30.6%和59.8%;成熟期有效穗数、地上部干物质量和水稻产量均表现为EC_2EC_1EC0,EC_2和EC_1处理的有效穗数分别比EC0处理多41.5%和21.3%、地上部干物质量分别比EC0处理高47.7%和25.8%、产量分别比EC0处理高35.4%和34.3%。由此可见,施用蚯蚓粪便可促进水稻分蘖,提高水稻叶面积、SPAD值和地上部干物质量,进而提高水稻籽粒产量。  相似文献   

Correlation between Mnk2 protein expression and prognosis of patients with esophageal squamous cell carcinoma     

ZENG Bo  FU Min-yi  FENG Yan-fen  HAN Xiang-qian  ZOU Da-wei  LUO Hong-he  LEI Yi-yan 《园艺学报》2017,33(10):1864-1868

AIM: To investigate the protein expression of mitogen-activated protein kinase-interacting kinase-2 (Mnk2) and its prognostic effect in the patients with resected esophageal squamous cell carcinoma (ESCC). METHODS: A total of 86 informative patients with surgically resected ESCC and 54 normal esophageal tissues were enrolled. Western blot and immunohistochemistry (IHC) were utilized to assess the protein expression of Mnk2, and its correlation with prognosis was statistically analyzed by the methods of Kaplan-Meier curve and Cox proportional hazard mode. RESULTS: The protein expression of Mnk2 was elevated in most of tumor tissues compared with the adjacent tissues. Clinicopathologic analysis showed that Mnk2 expression was significantly correlated with the TNM stage (P<0.05). Both disease-free survival (DFS) and overall survival (OS) of Mnk2 over-expression patients were shorter than those in Mnk2 negative expression group. Multivariate analysis confirmed that Mnk2 expression, as an independent and significant factor for both DFS and OS, predicted a poor prognosis of the patients with resected ESCC (P<0.05). CONCLUSION: The expression of Mnk2 was significantly related to the TNM stages, and might be a novel predictor for prognosis in ESCC.  相似文献