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不同基质栽培对番茄叶片光合作用和根系ATP酶的影响 总被引:1,自引:0,他引:1
以腐熟玉米秸、麦秸、菇渣、锯末等农产废弃物添加土壤后配成有机基质进行番茄栽培试验,探讨不同基质栽培对番茄叶片光合特性和根系ATP酶活性的影响。结果表明,有机基质栽培较土壤栽培植株同化产物积累增加,番茄光合速率、细胞间隙CO2浓度、呼吸速率均显著提高。有机基质栽培Fv/Fm、ΦPSⅡ、qP显著升高,P-H++ATPase、P-Ca2+-ATPase、V-H+-ATPase、V-Ca2+-ATPase活性提高。有机基质栽培番茄通过提高根系质膜和液泡膜ATPase活性、增加番茄的光合速率、细胞间隙CO2浓度,提高Fv/Fm、ΦPSⅡ、qP,提高光化学效率,增强营养吸收,从而促进番茄生长和同化物形成。 相似文献
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甘蓝型油菜显性核雄性不育基因的AFLP标记鉴定 总被引:2,自引:0,他引:2
甘蓝型油菜双基因显性细胞核雄性不育系统受一对显性不育基因和一对显性上位抑制基因共同控制,不育单株的基因型为MsMsrfrf和Msmsrfrf。为了在育种过程中高效筛选后代单株基因型,本研究利用一个显性细胞核雄性不育杂合两型系70056AB(Msmsrfrf×msmsrfrf)构建的兄妹交分离群体,采用AFLP技术,在480对AFLP引物中筛选到一个与不育基因紧密连锁的分子标记P9M14-130。利用P9M14-130引物对70056AB的88个单株进行检测,发现该标记与雄性不育基因间的交换率为1.1%。 相似文献
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利用大豆主要过敏原Gly m Bd 30K蛋白抗原表位蛋白为免疫原免疫BALB/c小鼠,取免疫小鼠脾细胞与小鼠骨髓瘤NS-1细胞融合.采用半固体培养基法和有限稀释法相结合的方法快速筛选获得稳定分泌的特异性杂交瘤细胞,用杂交瘤细胞株诱生小鼠腹水,应用蛋白A亲和层析法进行抗体纯化.采用Ig类与亚类鉴定试剂盒鉴定该单克隆抗体... 相似文献
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JIA Yan # YANG Liang # ZOU Detang QU Zhaojun WANG Jingguo LIU Hualong WANG Jin ZHAO Hongwei 《中国水稻科学》1986,34(5):443-456
【Objective】Our goal is to develop disaster-reducing, production-preserving and chemical-controlling cultivation techniques to effectively resist cold damage at the booting stage of japonica rice in cold region.【Method】The split plot design and the central combination test design were used to screen out the optimal application concentrations of CaCl2, glutamic acid(Glu), and γ-aminobutyric acid(GABA) through the membership function values of yield-related indicators under cold-water stress at booting stage.【Result】1) Under cold water stress at the booting stage, the optimal concentrations of exogenous CaCl2, Glu and GABA (CGG) were 1.87 mmol/L, 2.76 mg/L, 4.40 mmol/L, respectively and the average membership function value is the largest (0.932). 2) Compared with normal irrigation, the nitrogen photosynthetic efficiency of cold-region japonica rice decreased under cold stress at the booting stage. Compared with cold water stress at the booting stage, CGG application at the optimal concentration significantly increased the leaf area index (5.37%) and nitrogen photosynthetic efficiency (4.53%) of Dongnong 428 at the full heading stage; significantly increased leaf biomass per leaf area (2.56%) and leaf area index (3.39%) of Songjing 10 at the full heading stage. 3) Compared with cold water stress at the booting stage, the application of the optimal concentration of CGG increased the dry matter accumulation from heading stage to maturity in the cold-region japonica rice, but it was still lower than that of the normal irrigation, and there were differences between varieties and treatments. 4) Compared with cold water stress at booting stage, the yield and yield components of cold-region japonica rice increased with the application of the optimal concentration CGG, and the impact on cold-sensitive varieties was more significant. 5) Compared with the cold water stress at the booting stage, the average increase in temperature production efficiency of Dongnong 428 and Songjing 10 was 5.55% and 23.72% under the optimal concentrations of CGG, respectively. The contribution rate of exogenous CGG to the gap of temperature production efficiency is 8.99%.【Conclusion】The application of the optimal concentration of CGG under cold water stress at the booting stage promoted leaf growth of cold-region japonica rice at the full heading stage and increased the population nitrogen photosynthetic efficiency, which was beneficial to dry matter accumulation and yield formation after heading stage, thereby reducing the loss of cold-region japonica rice under cold water stress at booting stage and improving the temperature production efficiency of cold-region japonica rice. 相似文献