Use of an indirect enzyme-linked immunosorbent assay (elisa) in the detection of channel catfish, Ictalurus punctatus (Rafinesque), antibodies to Edwardsiella ictaluri     

P. WATERSTRAT  J. AINSWORTH  G. CAPLEY 《Journal of fish diseases》1989,12(2):87-94

Abstract. The use of an indirect enzyme-linked immunosorbent assay ( elisa ) for the detection of channel catfish antibody to Edwardsiella ictaluri is described. Changes in agglutination titre in fish immunized with Edwardsiella ictaluri heat killed whole bacterins or lipopolysaccharides were reflected by corresponding changes in elisa readings. Relatively high correlations were observed among elisa OD readings, computed elisa titres and corresponding agglutination titres.  相似文献   

Serum amyloid A protein (SAA) in horses: objective measurement of the acute phase response   总被引：1，自引：0，他引：1  

M B Pepys  M L Baltz  G A Tennent  J Kent  J Ousey  P D Rossdale 《Equine veterinary journal》1989,21(2):106-109

A sensitive and precise immunoassay for equine serum amyloid A protein (SAA) was established and used to determine, for the first time, the circulating concentration of this protein in health and disease. As in other species, equine SAA was present only at trace levels in healthy animals but behaved as an extremely sensitive and rapidly responding acute phase reactant following most forms of tissue injury, infection and inflammation, objectively reflecting the extent and activity of disease. Measurements of SAA should make a significant contribution to diagnosis and management of viral and bacterial infection in horses, and routine serial assays could provide an objective criterion for monitoring prospectively the general health of horses in training and racing.  相似文献   

Incidence of microscopically detectable degenerative characteristics in skeletal muscle of turkey     

A Sosnicki  R G Cassens  D R McIntyre  R J Vimini  M L Greaser 《British poultry science》1989,30(1):69-80

1. The incidence of microscopically detectable degenerative characteristics in 5 skeletal muscles (m. pectoralis thoracicus, m. supracoracoideus, m. biceps femoris, m. semitendinosus, m. femorotibialis medius) of turkeys was investigated. 2. Samples were obtained from 30 Large White turkey males 14, 16 and 18 weeks old. Hyaline degeneration, infiltration of mononuclear cells and necrotic fibres were observed. 3. Individual fibres varied greatly in size and muscle fibre nuclei were often shrunken and pyknotic. 4. Weak and/or uniform reaction for Ca++-ATPase and SDH in all types of muscle fibres and loss of alkaline phosphatase activity in cell membranes were noted. A positive reaction for acid phosphatase occurred in regions of perivascular infiltration and in necrotic muscle fibres. The majority of muscle fibres possessed high activity for phosphorylase a and b. 5. Based on the use of fluorescein alpha-bungarotoxin conjugate, motor end-plates appeared to be morphologically intact. Direct immunofluorescence with anti-chicken IgG showed positive reaction in muscle fibres undergoing necrosis and in the involved connective tissue. 6. Degenerative changes varied with age and were most marked in the oldest birds. 7. Because gross degenerative symptoms were absent from both the birds and the meat from them, the condition appears to be either different from or a precursor to the degenerative myopathy characterised by other authors.  相似文献   

Filtration of Green Algae and Cyanobacteria by Freshwater Mussels in the Partitioned Aquaculture System     

Kevin R.  Stuart Arnold G.  Eversole David E.  Brune 《Journal of the World Aquaculture Society》2001,32(1):105-111

Abstract.— The freshwater mussel Elliptio complanata was provided water containing green algae and cyanobacteria delivered from the Partitioned Aquaculture System (PAS) at eight flow rates to determine algal filtration rates as mg of particulate organic carbon (POC)/kg wet tissue weight per h. The dominant taxon in cyanobacterial waters was Microcystis while the dominant taxa in green algal waters were Scenedesmus and Ankistrodesmus . The cell counts of Scenedesmus and Ankistrodesmus were the only algal taxa that were significantly different between the incoming water and water filtered by mussels. Filtration rates of POC obtained from green algal water were significantly greater than from cyanobacteria-dominated waters at all flow rates. A significant increase in mean filtration rate was observed as flow rates increased. The filtration rate of green algae increased as POC concentration increased, peaking at 28 mg C/L. A maximum filtration rate was not observed with cyanobacterial waters.  相似文献   

Neurotrophins and the Thymus: Morphological Analysis of Mice Carrying a Non-functional Mutation on the Trka and Trkb Genes     

German&#;  A.  Laur&#;  R.  Levanti  M.B.  Montalbano  G.  German&#;  G.P.  Abbate  F.  Ciriaco  E. 《Veterinary research communications》2004,28(1):189-192

Veterinary Research Communications -  相似文献   

Hyperadrenocorticism associated with excessive sex hormone production by an adrenocortical tumor in two dogs.     

H M Syme  J C Scott-Moncrieff  N G Treadwell  M F Thompson  P W Snyder  M R White  J W Oliver 《Journal of the American Veterinary Medical Association》2001,219(12):1725-8, 1707-8

An 11-year-old spayed female Labrador Retriever and a 9-year-old castrated male miniature Poodle were evaluated because of clinical signs of hyperadrenocorticism. Cortisol testing did not support a diagnosis of hypercortisolemia in either dog; however, imaging studies revealed unilateral adrenal tumors in both dogs. Serum concentrations of 17-hydroxyprogesterone, progesterone, and estradiol were high in both dogs, and androstenedione concentrations were also high in 1 dog. It is suspected that sex hormone secretion by the adrenal tumors in these dogs resulted in clinical signs of hyperadrenocorticism. Clinical signs and hormonal abnormalities resolved in the male dog after surgical resection of the tumor. There was no improvement in clinical signs after treatment with mitotane in the female dog, which died 2 months after diagnosis. Histologic evaluation confirmed the presence of adrenocortical carcinoma in both dogs.  相似文献   

Hypercalcaemia associated with chronic lymphocytic leukaemia in a Giant Schnauzer.     

M Kleiter  R Hirt  G Kirtz  M J Day 《Australian veterinary journal》2001,79(5):335-338

A 7-year-old male Giant Schnauzer was referred with a history of severe vomiting, lethargy, weight loss, polydipsia and polyuria. Detailed investigations revealed leucocytosis with a marked lymphocytosis, mild non-regenerative anaemia, thrombocytopenia, hypercalcaemia and azotaemia. Circulating lymphocytes were small and well-differentiated, and the same lymphoid population was present in bone marrow. Chronic lymphocyctic leukaemia with associated paraneoplastic hypercalcaemia was diagnosed. Immunohistochemical staining of a bone marrow biopsy revealed a neoplastic B-cell line expressing CD79. The dog responded to therapy with prednisolone and chlorambucil for a period of 8 months.  相似文献   

Effects of Benzyl Adenine and Abscisic Acid on Grain Yield and Yield Components in Triticale and Wheat     

T. Sivakumar  Virendranath  & G. C. Srivastava 《Journal of Agronomy and Crop Science》2001,186(1):43-46

Grain yield and yield components (grains per ear, grain weight, 1000-grain weight, ear weight, ear seed ratio and dry matter partitioning between ear and seed) were examined in a wheat genotype (PBW-343) with well-filled grains and a Triticale genotype (DT-46) with poorly filled grains (showing grain shrivelling) grown in pots. Six days after anthesis (DAA), benzyladenine (BA) @2 µg ear⁻¹ and abscisic acid (ABA) @4 µg ear⁻¹ were injected at the base of the mother shoot ear in both species. It was observed that, in both wheat and Triticale , BA increased the grain weight, grain number and partitioning of dry matter between ear and seed, whereas ABA decreased the grain weight, grain number and dry matter partitioning between ear and seed. However, these decreases were slower in Triticale than in wheat. BA treatment increased the grain dry matter accumulation, which in turn resulted in better filling of grains and increased the grain weight in both wheat and Triticale . The average grain weight of Triticale was lower than that of wheat. Thus, it appears that variation in grain weight between wheat and Triticale might be due to different availabilities of growth-promoting phytohormones such as cytokinins and assimilates.  相似文献   

有摩擦的高速气体管流     

朱文重 《湖南农业大学学报(自然科学版)》1990,17(2)

本文以多变热力过程为基础建立了有摩擦管路的计算公式,并对计算方法和有关问题作了分析说明.  相似文献   

Survey of tick infestation and tick-borne ehrlichial infection of dogs in Ishigaki Island, Japan.     

H Inokuma  G Nane  T Uechi  Y Yonahara  P Brouqui  M Okuda  T Onishi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(11):1225-1227

Twelve (54.5%) of 22 free-roaming dogs in Ishigaki Island had tick infestation identified as Rhipicephalus sanguineus. There were 121 ticks recovered and consisted of 28 females, 58 males, 22 nymphs and 3 larvae. Infection of dogs possibly with canine ehrlichial pathogens was examined by both indirect immunofluorescence assay and polymerase chain reaction (PCR). Two dogs of the 13 examined were sero-positive for the human granulocytic ehrlichia agent, and one of two dogs was PCR positive for Ehrlichia platys. This dog had platelet numbers slightly lower than normal value, however, no morulae were found within platelet on peripheral blood smear stained with Giemsa.  相似文献