澧水常德段水环境质量现状分析与评价   总被引：1，自引：0，他引：1  

唐嶷  戈蕾  高铁军  吴岱镳  葛大兵 《广西农业科学》2010,41(1):52-54

比照《地表水环境质量标准》（GB3838—2002），运用综合评价指数法，对2007年度澧水常德段石门县三江口、津市市窑坡渡、安乡县沙河口3个监测断面的水质现状进行分析与评价。结果表明：澧水常德段水质总体上达到地表水Ⅲ类标准，水质良好，其中石门县三江口和安乡县沙河口所有监测项目均处于标准值范围，津市市窑坡渡存在粪大肠菌群超标现象。今后应加强水环境监测能力建设，重视水资源合理开发利用，统筹安排生产、生活用水，严格控制排污量，为地方经济社会发展提供可持续的水利保障。  相似文献   

高校图书馆核心竞争力研究     

徐大勇  于春莉  葛艳 《农业图书情报学刊》2012,24(5):5-6,11

介绍图书馆核心竞争力的概念,阐述高校图书馆培育核心竞争力的必要性,并从信息资源建设、人才队伍建设、技术创新、管理创新和图书馆文化建设等方面提出构建高校图书馆核心竞争力的策略。  相似文献   

10株牛源非结核分枝杆菌16S rRNA基因序列分析   总被引：1，自引：0，他引：1  

葛淑敏  钱爱东  王铁风 《中国预防兽医学报》2010,32(11)

本研究应用结核分枝杆菌的16S rDNA序列分析方法,鉴定非结核分枝杆菌菌株。应用PCR方法从10株牛源非结核分枝杆菌中扩增16S rDNA基因5'端片段并测序。用DNA分析软件将所获得的序列与GenBank中分枝杆菌序列相比较,计算种间相似性,构建系统发育树,对菌株进行分类与鉴定。结果显示,10株牛源非结核分枝杆菌中浅黄分枝杆菌1株;偶发分枝杆菌1株;母牛分枝杆菌2株;新金色分枝杆菌4株;另有2株N8和N9分别与新金色分枝杆菌和偶发分枝杆菌有较好的亲缘关系。检测结果提示,非结核分枝杆菌在牛群中存在应引起重视,而且它们对人畜的致病性需要进一步研究,以便采取有效的防制措施确保人类及畜群的健康。  相似文献   

Effects of High Fluoride and Low Iodine on Thyroid Function in Offspring Rats     

GE Ya-ming  NING Hong-mei  GU Xin-li  YIN Mei  YANG Xue-feng  QI Yong-hua  WANG Jun-dong 《中国农业科学(英文版)》2013,(3):502-508

Thirty-two Wistar rats were divided randomly into four groups of eight with six females and two males in each group. The rats were exposed to high fluoride drinking water (45 mg F- L-1 from 100 mg NaF L-1), low dietary iodine (0.0855 mg kg-1), or both together in order to assess the effects of these three regimens on the thyroid function of the offspring rats. After the animal model was established, the offspring rats were bred and 10-, 20-, 30-, 60-, and 90-d-old rats were used for the experiment. The treatments for the offspring rats were the same as those of their parents. In comparison with control rats, the relative thyroid glands were changed by three regimens, but the mean values of thyroid weight in the experimental groups saw no marked difference. Serum TT3 levels were increased in all stages in the low iodine (LI) group. In the high fluoride (HiF) group, increase in TT3 levels was observed except in 20-d-old rats. Decrease in TT3 at 20- and 90-d and increase in TT3 at 30- and 60-d were found in HiF+LI group. Serum TT4 levels first saw an increase, and then dropped in the LI and HiF+LI group. However, an increase in TT4 was found in the HiF group. The levels of TSH in serum rocketed at d 20, and then dropped in the next stages in experimental groups. The results suggested that thyroid disorder could be induced by high flroride in drinking water, low iodine diet, or both of them. Exposure time to fluoride or low iodine diet was one of the important factors that fluoride can induce the development of thyroid dyfunction.  相似文献   

Bioprospection of Microalgae and Cyanobacteria as Biocontrol Agents Against Vibrio campbellii and Their Use in White Shrimp Litopenaeus vannamei Culture     

Oscar González‐Davis  Elizabeth Ponce‐Rivas  M. Del Pilar Sánchez‐Saavedra  María‐Enriqueta Muñoz‐Márquez  William H. Gerwick 《Journal of the World Aquaculture Society》2012,43(3):387-399

Vibrio spp. are the most common and harmful shrimp pathogenic bacteria; however, microalgae and cyanobacteria have the ability to produce antimicrobial substances against these species. In this study, the organic and aqueous extracts of 28 species of marine microalgae and cyanobacteria were screened against Vibrio campbellii M1. Two of these phytoplankton species with antibacterial activity in aqueous extracts (Dunaliella tertiolecta and Skeletonema costatum) and nontoxic to brine shrimp Artemia franciscana nauplii were used to evaluate their anti‐Vibrio effect when used as green‐water cultures in Vibrio‐challenged white shrimp Litopenaeus vannamei cultures. No differences in mortality of juvenile L. vannamei were observed between treatments tested, suggesting that the pathogenicity of V. campbellii could be related to the growth stage of shrimp. The proximal composition of D. tertiolecta and S. costatum was in the recommended range for penaeid shrimp nutrition, allowing shrimp supplemented with these microalgae to have significantly greater total length and weight than control shrimp. Shrimp supplemented with S. costatum presented the highest values of organic mass (11.48 mg/organism) and growth rate (0.31 mg/d) in comparison to D. tertiolecta. These results indicate that microalgae are not only capable of producing antibacterial compounds against Vibrio but can also help shrimp nutrition.  相似文献   

禽流感病毒PB2亚单位抗血清的制备     

葛叶  邓国华  李雪平  陈化兰 《中国兽医学报》2010,30(6)

采用RT-PCR方法分段扩增PB2基因,将目的基因定向克隆至含HisTAG的原核表达载体pET-32a,经序列验证正确后,转化BL21大肠杆菌,诱导表达重组蛋白。靶蛋白经Ni+柱纯化后与弗氏免疫佐剂混合免疫新西兰大耳白兔,获得高效价的抗PB2的抗血清。Western-blot检测结果证实制备的抗血清可与PB2蛋白发生特异性反应。进一步将PB2全长基因准确插入真核表达载体pCAGGS,转染293T细胞表达PB2蛋白,通过IFA检测发现,抗血清与细胞表达的PB2蛋白发生反应,出现特异性荧光,经激光共聚焦检测PB2亚单位只在细胞核中出现荧光,说明PB2亚单位单独表达仅限于细胞核内。上述研究为进一步探索PB2亚单位的生物学特性及其结构与功能研究打下基础。  相似文献   

共生论及其在图书馆中的应用     

王青  王本永  梁丽珍  葛升平 《农业图书情报学刊》2012,24(11):64-66

通过对馆员与读者关系的分析,认为两者之间存在共生关系,并且是互利共生关系。这一认识有助于馆员主动改进工作。如设法满足读者需求,以馆员的服务去影响读者。改善馆员与读者关系的同时,提高图书馆服务效益。  相似文献   

基于扩散指数的中国大豆需求量增长率预警分析     

焦扬  葛慧玲  李周密  赵静朦 《东北农业大学学报》2013,(8):138-142

预警是对中国大豆市场的波动情况进行预报的有效手段。采用中国大豆的需求量增长率作为警情指标,利用1995~2010年的经济数据,选择49个警兆指标。通过时差相关分析法,去除无效指标后,将警兆分为先行、同步和滞后指标,并计算各类指标的权重。利用扩散指数编制先行、同步、滞后指标的预警波动曲线,与大豆需求量增长率曲线比较后发现,先行与同步指标有效,滞后指标稳定性稍差。通过先行指标对2011、2012中国大豆需求量增长率进行验证,证明方法有效性,及为未来的2~3年中国大豆需求量增长率预警奠定理论基础。  相似文献   

应用TaqMan-MGB探针进行松材线虫的实时荧光定量检测技术研究   总被引：7，自引：0，他引：7  

葛建军  曹爱新  刘先宝  陈长法 《植物病理学报》2005,35(Z1):52-58

 松材线虫是国际公认的最重要的检疫性有害生物之一,也是我国2类检疫危险性有害生物,我国口岸多次从货物的木质包装中截获该线虫。由于松材线虫与拟松材线虫在形态上极其相似,难以区分,幼虫更无法用于鉴定。传统的形态学鉴定、生化以及其他分子技术等方法存在费时、准确度不高、灵敏度低等缺点,不易形成标准。我们设计筛选一对引物以及一条MGB探针,对松材线虫进行实时荧光PCR检测。建立了一条从1 pg到10⁴pg标准曲线,相关系数r=0.965。该检测方法省时、准确、快速、无污染。  相似文献   

昆虫雌性信息素在害虫种群监测和大量诱捕中的应用与讨论   总被引：21，自引：4，他引：21  

苏建伟  肖能文  戈峰 《植物保护》2005,31(5):78-82

分析了早期应用昆虫性信息素效果不佳的原因、现代性信息素技术的优点;综述了应用性信息素技术监测昆虫种群和应用性信息素技术诱捕防治害虫的效果、局限性及其未来应用的前景。  相似文献