Study of Red Wine Neuroprotection on Astrocytes     

M. Pilar Gómez-Serranillos  Sara Martín  Teresa Ortega  Olga M. Palomino  Marín Prodanov  Visitación Vacas  Teresa Hernández  Isabel Estrella  M. Emilia Carretero 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(4):238-243

Phenolic composition of wine depends not only on the grape variety from which it is made, but on some external factors such as winemaking technology. Red wine possesses the most antioxidant effect because of its high polyphenolic content. The aim of this work is to study for the first time, the neuroprotective activity of four monovarietal Spanish red wines (Merlot (ME), Tempranillo (T), Garnacha (G) and Cabernet-Sauvignon (CS)) through its antioxidant ability, and to relate this neuroprotection to its polyphenolic composition, if possible. The wine effect on neuroprotection was studied through its effect as free radical scavenger against FeSO₄, H₂O₂ and FeSO₄ + H₂O₂. Effect on cell survival was determined by 3(4,5-dimethyltiazol-2-il)-2,5-diphenyltetrazolium reduction assay (MTT) and lactate dehydrogenase (LDH) release assay on astrocytes cultures. Results showed that most of the studied wine varieties induced neuroprotection through their antioxidant ability in astrocytes, Merlot being the most active; this variety is especially rich in phenolic compounds, mainly catechins and oligomeric proanthocyanidins. Our results show that red wine exerts a protection against oxidative stress generated by different toxic agents and that the observed neuroprotective activity is related to their polyphenolic content.  相似文献   

Applicability of lignins from different sources as antioxidants based on the protective effects on lipid peroxidation induced by oxygen radicals     

Vanessa Ugartondo  Montserrat Mitjans  María Pilar Vinardell   《Industrial Crops and Products》2009,30(2):184-187

Increasing evidence suggest that excessive concentrations of reactive oxygen species in the human body are involved in a number of pathological events and that oxidative damage to cell components may play an important pathophysiological role in many types of human diseases. Several studies have shown the possible benefits of antioxidants from plant sources in altering, reversing or forestalling the negative effects of oxidative stress.The study was designed to evaluate whether lignins prevent H₂O₂-induced lipid peroxidation. The protective effect of industrial lignins obtained from different sources against hydrogen peroxide induced oxidation was investigated in normal human red blood cells (RBCs). RBCs, preincubated with different amounts of lignins and challenged with hydrogen peroxide were analyzed for lipid peroxidation. The high molecular weight can be considered as one of the main factors decreasing the radical scavenging activity. When comparing different lignins the inhibitory effects against lipid peroxidation were notably related to their molecular weights. In this sense bagasse with the lowest Mn was the greatest antioxidant and lignosulfonate with the highest Mn was the lowest antioxidant lignin.These results open the possibility of new applications of lignins. The potential health benefits and industrial applications of lignins make them a promising research resource.  相似文献   

黑龙江省玉米生产效率研究   总被引：7，自引：2，他引：5  

赵贵玉  张越杰  葛世萍 《玉米科学》2009,17(4):139-143

运用非参数的HMB指数方法,利用1991～2005年黑龙江省玉米生产的投入产出的面板数据(Panel data),对玉米生产的全要素生产率进行实证分析。结果表明,黑龙江省玉米生产率的增长过程比较明显,呈先升后降的趋势,技术进步和混合效率的增长是促进全要素生产率增长的主要原因,规模效率的变化对玉米生产率的增长起了阻碍作用。  相似文献   

湖北省66份小麦品种(系)赤霉病抗性鉴定与分子检测     

徐晴  郑丹  许甫超  秦丹丹  董建辉  葛双桃  朱展望  董静 《麦类作物学报》2024,(4):432-441

赤霉病是由禾谷镰刀菌引起的小麦穗部病害,严重危害小麦生产,抗赤霉病品种选育是减轻其危害的重要途径之一。本研究以湖北省不同时期审定的59个小麦品种和选育的7份优异品系为材料,采用喷雾接种对其进行田间赤霉病抗性鉴定,并利用抗性基因Fhb1功能性分子标记和主效抗性基因(Fhb2、Fhb4、Fhb5和QFhs.crc-2DL)连锁分子标记对供试材料进行检测,分析其遗传分布和利用状况;同时分析湖北小麦品种(系)赤霉病抗性、株高和小穗密度等性状年代间的差异。结果表明,16份供试材料赤霉病抗性水平达到中抗,占比24.2%,以810619品系抗性最好,病情指数略低于苏麦3号;42份材料达到中感,占63.6%。分子检测结果显示,仅鄂T45048携带Fhb1;鄂麦11、鄂麦18和810619等29份材料(43.9%)可能携带Fhb5和QFhs.crc-2DL单个或2个抗性基因,说明这2个抗性基因在湖北小麦赤霉病抗性育种中得到了较多应用。赤霉病抗性与株高的相关性分析结果显示,近15年湖北小麦品种株高持续降低,但其与赤霉病抗性无显著相关。研究结果为明确湖北小麦品种赤霉病抗性水平和分子遗传基础提供了参考。  相似文献   

抗猪流行性腹泻病毒N蛋白单克隆抗体制备及部分特性鉴定   总被引：3，自引：2，他引：1  

裴敦国  葛俊伟  马广鹏  姜艳萍  李一经 《东北农业大学学报》2008,39(7)

以原核表达系统pGEX-6p-1-N/BL21经IPTG诱导表达的重组PEDV N-GST融合蛋白作为免疫抗原,免疫BALB/c小鼠,运用淋巴细胞杂交瘤技术,制备单克隆抗体,用原核表达系统T12-pPROHTa/BL21经IPTG诱导表达的PEDV N蛋白做筛选抗原,通过间接ELISA进行筛选,获得两株抗PEDV N蛋白杂交瘤细胞,命名为2E3、4C6。亚类鉴定为IgG1和IgG2a型,均为к链抗体,染色体数平均为(91±7)对。用制备的单抗与pGEX-6p-1-N/BL21、T12-pPROHTa/BL21诱导表达后的菌体裂解物做Western Blot试验,在不同载体表达的N蛋白处出现明显的特异性条带;通过间接ELISA做病原检测,这两株单抗不与TGEV、PRV和PrV发生交叉反应,而与PEDV显示良好的特异性反应。  相似文献   

Effect of cultivation patterns on field arthropod community of Alisma orientalis (Sam.) Juzep.     

GE You-mao  LU Gang  ZHANG Hong 《福建农业学报》2008,23(3)

  相似文献   

新疆红花主要栽培品种遗传多样性的RAPD分析(英文)   总被引：5，自引：1，他引：4  

岳庆妮  葛娟  王蕾  张霞  王绍明  王建明 《农业科学与技术》2008,9(4):34-38

[Objective] Study on the genetic diversity in main cultivars of safflower distributing in Xinjiang Uighur Autonomous Region by means of RAPD makers.[Method] Genomic DNAs of 29 safflower accessions from Xinjiang Uighur Autonomous Region were extracted for PCR amplification using 20 RAPD primers.[Result] Totally 156 bands were amplified,among which 144 bands were polymorphic(accounting for 92.31%),indicating that safflower is endowed with plentiful genetic diversity.Based on the DNA fingerprint,the 29 safflower accessions were grouped into four populations,the classification results may be not related with ecological regionality.[Conclusion] RAPD technique is an available tool to analyze the genetic diversity of safflower germplasm at molecular level.  相似文献   

猪皮肤组织块冷冻保存方法的研究   总被引：1，自引：0，他引：1  

程旭梅  倪黎纲  吴晓伟  孙鹏翔  葛剑辉  戴建明  李碧春 《扬州大学学报(农业与生命科学版)》2008,29(3)

以二甲基亚砜、甘油和乙二醇为冷冻保护剂,以3种浓度(10%、20%、30%)在2种降温条件下探讨适合猪耳皮肤组织冷冻保存的最适条件和方法以及复苏后对皮肤组织生长能力的影响.结果表明: 快速冷冻条件下各处理均无组织块生长.慢速冷冻条件下复苏后组织块的存活率均为100%, 各冷冻保护液3种浓度的平均生长率分别为14.29%、56.52%和8.70%, 差异显著.20%甘油保护液下采用慢速冷冻方法复苏后组织块的生长率达82.35%, 极显著高于其他组和对照组.新鲜和冻存后的细胞培养均采用组织块培养法, EDTA-牛血浆贴覆, 97.78%的新鲜样本组织块能较好生长,且扩展能力强.  相似文献   

高校代扣代缴个人所得税探讨     

戈红 《安徽农业大学学报》2008,(6):66-67,83

代扣代缴是高校个人所得税征收的主要方式。目前高校的代扣代缴工作存在着量大、程序复杂、信息化建设薄弱、税收管理手段落后等问题,通过建立纳税人与扣缴义务人双向申报制度、实行征管手段的信息化,加强全员全额管理、积极做好高校个人所得税的税收筹划等途径可以强化个人所得税代扣代缴工作。  相似文献   

不同年份地下水位大麦参数模型与产量动态模拟     

葛羽屏  崔慧芳 《农业网络信息》2008,(7)

本文研究了不同地下水位下大麦生长期叶面积指数、地上部各营养器官、储藏器官、总生物量干重的变化动态,植株各器官的干物质积累量和分配率,不同时期植株各器官的氮素含量和可溶性糖含量的变化动态;运用模型对大麦各器官干物质重及产量动态进行了模拟,并对模拟结果与实测结果进行了相关性分析,最后对大麦产量进行了模拟,结果得到各个不同年份不同地下水位处理大麦的产量与降水量之间存在着比较明显的负相关.  相似文献