小麦耐盐相关基因TaSTK的克隆   总被引：4，自引：0，他引：4  

葛荣朝  赵宝存  陈桂平  秘彩莉  沈银柱  黄占景 《作物学报》2007,33(5):857-860

利用RACE方法,从小麦耐盐突变体RH8706-49中扩增获得小麦丝氨酸/苏氨酸蛋白激酶（Triticum aestivum serine-threonine protein kinase）基因TaSTK的全长cDNA序列,含1 958 bp,其中开放阅读框1 431 bp,编码476个氨基酸。其编码区基因组DNA全长为4 095 bp,含5个外显子。该基因的全长cDNA序列及基因组序列均已提交GenBank数据库（登录号：DQ103756和DQ341377）。经过NCBI比对发现该基因的氨基酸序列与丝氨酸/苏氨酸蛋白激酶、酪氨酸蛋白激酶、蛋白激酶都具有较高的同源性。Northern杂交检测表明,TaSTK在小麦中属于盐诱导增强型基因,并且在耐盐材料RH8706-49中诱导增强程度高于敏盐材料H8706-34。TaSTK的杂交信号非常弱,表明TaSTK在小麦幼苗的叶片组织中属于低表达的基因类型。  相似文献   

Growth versus storage: responses of Mediterranean oak seedlings to changes in nutrient and water availabilities     

Virginia Sanz Pérez  Pilar Castro Díez  Fernando Valladares 《Annals of Forest Science》2007,64(2):201-210

We compare dry mass (DM) and storage of starch (St) and nitrogen (N) in seedlings of three Mediterranean oaks, two evergreens (Quercus coccifera L. and Q. ilex L. subsp. ballota (Desf.) Samp) and one deciduous (Q. faginea Lam.), across different scenarios of nutrient and water availabilities. Three fertilization (5, 50 and 200 mg of N per plant and growing period) and watering (28–39, 55–71 and 70–85 g H₂O 100 g _soil ^?1 gravimetric soil water) treatments were applied to current-year seedlings between May and October 2002 in two independent experiments. The three species showed a similar response to fertilization, storing nitrogen instead of increasing biomass, in agreement with adaptations to nutrient-poor habitats. However, they differed in their responses to water, reflecting the different water requirements in the field: Q. coccifera, from arid zones, showed no response to water regarding DM and St; Q. faginea, from humid zones, required higher water availability to simultaneously increase growth and storage; while Q. ilex, spanning over most of the water availability range, exhibited a balanced increase of both functions when water increased moderately. In the two evergreen species, N concentration increased with water supply, whereas the reverse occurred in Q. faginea. The latter species favoured growth over storage at moderate water supply (according to its more competitive strategy), although it was the species which accumulated more St and N at the end of the experiments (autumn).  相似文献   

油葵叶片离体再生体系的建立   总被引：3，自引：1，他引：2  

胡文冉  黄乐平  孟庆玉  危晓薇  葛峰  李建平  黄全生 《新疆农业科学》2006,43(1):82-85

以油葵自交种7B7的无菌苗子叶为诱导不定芽材料,MS培养基为基本培养基,研究油葵子叶组织培养有关影响因子.比较了不同培养基成分、不同碳源、不同附加物对不定芽分化的影响,确定出油葵自交种子叶离体诱导再生芽的最佳培养基:改良MS(MS大量、微量、铁盐、B5有机)+0.5; KNO3+0.05; CH+0.01; 肌醇+3; 葡萄糖+4 mg/L 6-BAP+6～9 mg/L AgNO3+2.6 g/L Phytagel,建立了油葵相对高效的再生体系.为油葵基因转化的研究奠定了基础.  相似文献   

Variation in the radial patterns of sap flux density in pubescent oak (Quercus pubescens) and its implications for tree and stand transpiration measurements     

Poyatos R  Cermák J  Llorens P 《Tree physiology》2007,27(4):537-548

Radial variation in sap flux density across the sapwood was assessed by the heat field deformation method in several trees of Quercus pubescens Wild., a ring-porous species. Sapwood depths were delimited by identifying the point of zero flow in radial patterns of sap flow, yielding tree sapwood areas that were 1.5-2 times larger than assumed based on visual examinations of wood cores. The patterns of sap flow varied both among trees and diurnally. Rates of sap flow were higher close to the cambium, although there was a significant contribution from the inner sapwood, which was greater (up to 60% of total flow) during the early morning and late in the day. Accordingly, the normalized difference between outer and inner sapwood flow was stable during the middle of the day, but showed a general decline in the afternoon. The distribution of sap flux density across the sapwood allowed us to derive correction coefficients for single-point heat dissipation sap flow measurements. We used daytime-averaged coefficients that depended on the particular shape of the radial profile and ranged between 0.45 and 1.28. Stand transpiration calculated using the new method of estimating sapwood areas and the radial correction coefficients was similar to (Year 2003), or about 25% higher than (Year 2004), previous uncorrected values, and was 20-30% of reference evapotranspiration. We demonstrated how inaccuracies in determining sapwood depths and mean sap flux density across the sapwood of ring-porous species could affect tree and stand transpiration estimates.  相似文献   

银黑狐种狐的选择     

葛东华 《黑龙江畜牧兽医》2000,(10):31

养殖珍贵毛皮动物目的是为了取皮.银黑狐皮是珍贵毛皮动物中的上等裘皮,但饲养的品种不同所产的裘皮质量和创造的经济效益也不同.因此,选择银黑狐留种或引种非常重要,种狐选好了,才能适应国内外市场的需求.  相似文献   

长白山中蜂与蜂巢包围物空间关系的研究   总被引：1，自引：0，他引：1  

葛凤晨  历延芳 《蜜蜂杂志》2000,(9):3-5

在长白山区观测调查了在7种不同蜂巢包围物中野生或家养的154群中蜂 ,发现大包围物中的蜂巢较大、群势较强 ,结成复脾蜂团 ,外勤蜂直接飞出飞入蜂团 ;而小包围物中的蜂巢较小、群势较弱。进一步证明 ,中蜂在进化过程中 ,某些方面像西蜂那样演变 ,而更多方面仍接近祖型[福建农学院 ,主编 .养蜂学[M] .福州 :福建科学技术出版社 ,1981 .30～31.]。长白山中蜂在大包围物中营造较大蜂巢、维持较大群势 ,系原始野生特性的延续 ,这种特性是中蜂改良饲养中不可忽视的生物学基础条件  相似文献   

Activities of defense-related enzymes induced by benzothiadiazole in rice to blast fungus Chinese Rice Research Newsletter     

GE Xiuchun  SONG Fengming  CHEN Yongye  ZHENG Zhong 《水稻科学》2001,9(4):10

  相似文献   

小麦T型细胞质雄性不育保持系线粒体DNA片段转化不育系的初步研究   总被引：3，自引：1，他引：2  

赵宝存  葛荣朝  沈银柱  黄占景 《华北农学报》2004,19(4):21-23

细胞质雄性不育在植物界普遍存在,其不育机理一直是人们研究的热点。采用花粉管通道法,利用T型细胞质雄性不育保持系75-3369B线粒体的基因文库,选取其中所载外源片段较大的重组子转化T型不育系小麦75-3369A,结果重组子ME252和ME317的转化后代变成可育,表明线粒体的部分片段可以使细胞质雄性不育系的育性发生转变。  相似文献   

新疆海岛棉茎尖培养初探     

李建平  葛峰  黄全生  王冬梅  孟庆玉 《新疆农业科学》2004,41(6):445-447

海岛棉的茎尖培养需要特定的适宜营养和激素条件,新海5号和K354在KT 0.5 mg/l和IAA 0.2 mg/l的MS培养基上适宜生长;新海16号在KT 1.0 mg/l和IAA 2.0 mg/l的MS培养基上生长分化良好.对比发现激素配合使用在一定浓度范围内有利于茎尖的生长分化.苗龄大小在一定程度上影响茎尖的生长分化进程和程度,3日、4日苗龄的茎尖生长分化速率明显高于2日、5日苗龄的茎尖,4日苗龄的茎尖分化程度又较3日苗龄的茎尖显著.  相似文献   

城市生活垃圾堆肥对春小麦生长和土壤的影响     

马琨  王兆骞  杜茜  戈乃玢  胡霭堂 《农业环境科学学报》2000,19(5)

利用垃圾堆肥作为肥料 ,采用大田试验对春小麦生长和土壤的影响进行了试验研究。结果表明 ,堆肥用量的春小麦试验中 ,每公顷施150t最好 ,产量增幅达25 %。施堆肥提高土壤有机质和养分供应力 ,土壤容重下降 ,偏碱性土壤pH向中性点靠拢。土壤重金属Cu、Zn全量 ,随堆肥用量而递增。作物体内Cu、Zn浓度有增加的趋势 ,表现为根系>籽粒>茎叶。  相似文献