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21.
Gomis SM Gomis AI Horadagoda NU Wijewardene TG Allan BJ Potter AA 《Tropical animal health and production》2000,32(6):341-351
Cellulitis caused by Escherichia coli in broilers results in substantial losses to the broiler industry in North America and Europe due to condemnations at slaughter. The objective of this study was to identify cellulitis in broilers in Sri Lanka and to characterize the E. coli from cellulitis and other colibacillosis lesions. Twenty-four farms from the low- and mid-country were selected and bacterial isolations were obtained from 241 birds. Two hundred and ninety-one gross lesions were observed in these 241 birds and 162 E. coli isolates were obtained. Cellulitis was observed in 21% of the birds. Twenty-one per cent of the birds had multiple lesions due to E. coli. The frequency of detection of other disease syndromes was 162 (67%) birds with pericarditis, 26 (11%) airsacculitis, 24 (10%) hepatitis, 12 (5%) perihepatitis, and 16 (7%) polyserositis (a combination of pericarditis, perihepatitis and airsacculitis). Serogroups O78, O2, O85 and O88 were distributed among the 32% of typable E. coli and 81% of isolates were assigned to three biotypes. Forty-four per cent of the E. coli isolates produced aerobactin and 88% demonstrated resistance to the bactericidal effect of normal chicken serum. The majority of the E. coli isolates were resistant to the antibiotics commonly used in poultry. All the E. coli isolates were non-haemolytic and 25% of the isolates produced K1 capsule. This study demonstrated the presence of cellulitis in Sri Lanka and this report describes some of the phenotypic characteristics of the E. coli isolates. 相似文献
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Acute phase proteins in cattle: discrimination between acute and chronic inflammation. 总被引：17，自引：0，他引：17
N U Horadagoda K M Knox H A Gibbs S W Reid A Horadagoda S E Edwards P D Eckersall 《The Veterinary record》1999,144(16):437-441
Acute phase proteins such as serum amyloid A, haptoglobin, and alpha 1-acid glycoprotein have been identified as markers of inflammation in cattle because they are produced by the liver in response to pro-inflammatory cytokines. This study was designed to assess whether they could be used to discriminate between acute and chronic inflammation. Their concentrations were measured in serum samples from 81 cattle in which inflammation was classified by thorough clinical examination, supported by postmortem findings, as being acute in severity in 31 and chronic in 50. The classical haematological markers of inflammation were also determined in blood from the animals. Serum amyloid A had a maximum (100 per cent) clinical sensitivity in discriminating between the acute and chronic cases, and haptoglobin had the highest clinical specificity of 76 per cent; counts of neutrophils and band neutrophils had sensitivities of 71 per cent and 42 per cent and specificities of 30 per cent and 72 per cent, respectively. It was concluded that serum amyloid A and haptoglobin may be used to discriminate between acute and chronic inflammatory conditions. 相似文献
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Efficiency of utilisation of different diets with contrasting forages and concentrate when fed to sheep in a discontinuous feeding pattern 总被引：1，自引：0，他引：1
The effects of feeding different levels of forages and concentrate, in a discontinuous feeding pattern, on the efficiency of feed utilisation and rumen function were studied using rumen fistulated sheep. Experiment 1 was a 4 × 4 latin square design to determine the whole tract digestibility and rumen characteristics of diets comprising 15% (C15), 25% (C25), 35% (C35) and 45% (C45) concentrate (energy-dense dairy pellets) with the rest of the diet being a combination of fresh short rotation ryegrass (Lolium mutiforum) and conserved (lucerne hay and maize silage) forages. In Experiment 2, the rumen degradation characteristics of feed ingredients were determined using the nylon bag technique. Daily dry matter intake (either expressed as g/kg LW or g/kg W 0.75) was 10% lower (p = 0.03) for the C15 diet compared with C25, C35 and C45 diets. The apparent in vivo digestibility of dry matter (DM) for C15 diet was 4% higher (p = 0.04) than the C35 and C45 diets which may be attributed to the high quality of the forage (ryegrass) used.Fibre digestibility decreased as proportion of concentrate in the diet increased. However, this was unlikely due to changes in the rumen fermentation pattern, as neither pH (6.1 ± 0.23) nor ammonia concentration (24.4 ± 6 mg/dl), were different (p > 0.05) among diets. Instead, the lower fibre digestion was most likely the result of different type and proportion of fibre among diets, as total rumen degradability and rate of fibre degradation in the rumen were higher (p = 0.001) for ryegrass than for other feedstuffs. There was no significant difference in total nitrogen balance and urinary allantoin excretion among diets, which indicated similar total microbial protein synthesis (MPS). The asynchrony observed, for N and energy availability in the rumen for different diets using Sinclair et al. [Sinclair, L.A., Garnsworthy, P.C., Newbold, J.R., Buttery, P.J., 1993. Effect of synchronizing the rate of dietary energy and nitrogen release on rumen fermentation and microbial protein synthesis in sheep. J. Agric. Sci. 120, 251–263] equation, was due to the feeding pattern used in this study leading to excess of N in relation to total organic matter digested in the rumen. In conclusion, feeding concentrates in the diets as PMR with conserved forages in a discontinuous feeding pattern may be valuable to develop feeding strategies in a pasture based system for high producing dairy cows without affecting the rumen system. 相似文献
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Effect of Manuka honey gel on the transforming growth factor β1 and β3 concentrations,bacterial counts and histomorphology of contaminated full‐thickness skin wounds in equine distal limbs 
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Horadagoda NU Hodgson JC Moon GM Wijewardana TG Eckersall PD 《Research in veterinary science》2002,72(3):194-200
Clinical changes and acute phase responses, including tumour necrosis factor-alpha (tnfalpha), in six buffalo calves were examined following intravenous inoculation of a bolus of endotoxin (1 microg kg(-1) bodyweight in 10 ml of phosphate-buffered saline [ pbs ]) extracted from Pasteurella multocida serotype B:2, the bacterium responsible for haemorrhagic septicaemia (hs) in Asia. Endotoxin injection caused a rapid onset of clinical signs characterised by dullness, sternal recumbency, elevated rectal temperatures, excessive salivation and dyspnoea that lasted for up to 12 hours post-inoculation (p.i.). Serum concentrations of tnfalpha rose within 1 hour p.i. to reach peak values ranging between 8 and 140 ng ml(-1) at 1-2 hours p.i. and then declined rapidly to baseline levels 3-5 hours p.i. Endotoxin injection induced other acute phase changes, including a rapid leucopenia and reductions in the serum concentrations of iron and zinc and a delayed but prolonged increase in haptoglobin from 12 hours p.i. that reached a plateau from about 60 hours p.i. Three control calves injected with 10 ml pbs showed no clinical or blood compositional changes. By reproducing key signs of hs the work confirms a pivotal role of endotoxin in the pathogenesis of hs and emphasises the exquisite sensitivity of the buffalo to P multocida endotoxin. 相似文献
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Toby J. Koenig BVSc Andrew J. Dart BVSc PhD Diplomate ACVS & ECVS Neil Horadagoda BVSc PhD Robin J. Bell BVSc MVSc Diplomate ECVS Nigel Perkins BVSc PhD Diplomate ACT Christina Dart Dr. Med. Vet. DVSc MVSc Mark Krockenberger BVSc PhD Leo B. Jeffcott BVetMedPhD DVSc Christopher B. Little BVSc PhD Diplomate ACVS 《Veterinary surgery : VS》2014,43(5):612-622