KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media     

Masafumi Katayama  Manabu Onuma  Tomokazu Fukuda 《The Journal of Poultry Science》2021,58(4):270

Cultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases in vitro while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and there are currently relatively few options for this basal medium (medium 199 and KAv-1). This means that there is still room for the development of an optimal basal medium for avian cell culture. Here we compare KAv-1 medium, Dulbecco''s modified Eagle medium (DMEM) and medium 199 during the culture of chick fibroblasts and determine that KAv-1 remains the optimal medium for these assays. Our results show that DNA damage is reduced in fibroblasts cultured in the KAv-1 medium, when compared to both DMEM and Medium 199 and that these cells also display improved growth dynamics in KAv-1 medium when compared to both DMEM and medium 199. To the best of our knowledge, this is the first study to describe a comparative analysis of culture media for avian cells, which would provide useful information for poultry scientists.  相似文献   

Quantitative structure–fungicidal activity relationships of N-(4-difluoromethoxybenzyl)­pyrimidin-4-amines against wheat and barley fungi     

Yoshinori Yamanaka  Masaru Moritomo  Katsutoshi Fujii  Toshinobu Tanaka  Yasuhisa Fukuda  Keiichiro Nishimura 《Pest management science》1999,55(9):896-902

A set of N-(4-difluoromethoxybenzyl)pyrimidinamines with various substituents at the 4- and 5-positions of the pyrimidine ring and at the benzyl position were prepared, and their fungicidal activities against wheat brown rust, Puccinia recondita, and barley powdery mildew, Erisiphe graminis, were measured. Variations in each of these activities were quantitatively analysed by the use of physicochemical substituent parameters and a regression analysis. Each of these activities was parabolically correlated with the steric bulkiness of the pyrimidine substituents and with the bulkiness or the hydrophobicity of the benzylic substituents. © 1999 Society of Chemical Industry  相似文献   

Dehydroeffusol Rescues Amyloid β25–35-Induced Spatial Working Memory Deficit     

Fukuda  Toshiyuki  Sato  Yuichi  Takiguchi  Mako  Yamamoto  Takahiro  Murasawa  Hiroyasu  Pawlak  Akiko  Kobayashi  Hiroyuki  Tamano  Haruna  Takeda  Atsushi 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(2):279-282

Plant Foods for Human Nutrition - Amyloid β (Aβ) peptides produced from the amyloid precursor protein, a transmembrane protein, are neurotoxic and blocking the neurotoxicity may lead to...  相似文献   

Interannual variation of the diet shifts and their effects on the fatness and growth of age‐0 Pacific bluefin tuna (Thunnus orientalis) off the southwestern Pacific coast of Japan     

Yuko Hiraoka  Ko Fujioka  Hiromu Fukuda  Mikio Watai  Seiji Ohshimo 《Fisheries Oceanography》2019,28(4):419-433

We investigated the effect of feeding habits on the fatness (length–weight relationship (LW)) and otolith growth of age‐0 Pacific bluefin tuna (PBF) (fork length (FL) of 15.0–29.9 cm) collected off the southwestern Pacific coast of Japan from July to August during 2010–2015. The feeding rate on Clupeiformes (FRc) was substantially influenced by the path of the Kuroshio Current during early summer. We suggest that the following patterns occurred in the feeding habits of PBF. In 2012 and 2014, the short distance from the coast to the Kuroshio provided high overlap between PBF and Clupeiformes and increased the opportunity for feeding, and this pattern likely caused the observed diet shift from Crustacea to Clupeiformes by smaller PBF in the studied years than those in other years. In 2011 and 2015, the encounter probability for Clupeiformes might have been low due to the long distance to the Kuroshio Current, which led PBF to actively consume non‐Clupeiformes Teleostei. In contrast, in August 2010 and 2015, PBF did not encounter Clupeiformes or other Teleostei and may have been forced to feed on a large number of Crustacea; this effect was likely due to high water temperatures. Despite the interannual fluctuation in the timing of the diet shift and the feeding intensity (FI), these factors did not contribute to growth in length; rather, they contributed to fatness. Considering size‐dependent mortality in the larval stage, these results suggest that only fast‐growing individuals survive, even if the feeding conditions drastically change.  相似文献   

Small-scale variation in feeding environments for the Manila clam <Emphasis Type="Italic">Ruditapes philippinarum</Emphasis> in a tidal flat in Tokyo Bay     

Satoshi Watanabe  Satoshi Katayama  Masashi Kodama  Naritoshi Cho  Kaoru Nakata  Masaaki Fukuda 《Fisheries Science》2009,75(4):937-945

The relative contribution of particulate organic matters (POMs) in water column and sediment as a food source for the Manila clam, Ruditapes philippinarum, was studied using carbon and nitrogen stable isotopic method (δ¹³C and δ¹⁵N) in a tidal flat at Seaside Park, Yokohama, Japan. Comparisons of δ¹³C and δ¹⁵N among R. philippinarum and POMs in surface water, bottom water, and sediment surface indicated that R. philippinarum larger than 5 mm shell length (SL) mainly assimilated benthic POM, and individuals smaller than 5 mm SL assimilated benthic and pelagic POM. Continuous measurements of chlorophyll concentrations in the bottom water revealed tide-driven resuspension of the benthic phytopigments. R. philippinarum showed differences in δ¹³C and δ¹⁵N along an inshore–offshore transect, indicating small-scale spatial differences in POM provision in the tidal flat. These findings suggest that POM in the bottom water, supposedly inhaled by R. philippinarum, is a mixture of a larger proportion of resuspended benthic POM and a smaller proportion of pelagic POM, and that the mixing ratio of the POMs may be affected by the hydrodynamics of flooding water associated with tidal flat topography.  相似文献   

Clonal structure in Ichthyobacterium seriolicida,the causative agent of bacterial haemolytic jaundice in yellowtail,Seriola quinqueradiata,inferred from molecular epidemiological analysis          下载免费PDF全文

T Matsuyama  Y Fukuda  T Sakai  N Tanimoto  M Nakanishi  Y Nakamura  T Takano  C Nakayasu 《Journal of fish diseases》2017,40(8):1065-1075

Bacterial haemolytic jaundice caused by Ichthyobacterium seriolicida has been responsible for mortality in farmed yellowtail, Seriola quinqueradiata, in western Japan since the 1980s. In this study, polymorphic analysis of I. seriolicida was performed using three molecular methods: amplified fragment length polymorphism (AFLP) analysis, multilocus sequence typing (MLST) and multiple‐locus variable‐number tandem repeat analysis (MLVA). Twenty‐eight isolates were analysed using AFLP, while 31 isolates were examined by MLST and MLVA. No polymorphisms were identified by AFLP analysis using EcoRI and MseI, or by MLST of internal fragments of eight housekeeping genes. However, MLVA revealed variation in repeat numbers of three elements, allowing separation of the isolates into 16 sequence types. The unweighted pair group method using arithmetic averages cluster analysis of the MLVA data identified four major clusters, and all isolates belonged to clonal complexes. It is likely that I. seriolicida populations share a common ancestor, which may be a recently introduced strain.  相似文献   

A pathologic study on the inhibitory effects of a herbal medicine against the glomerular lesion induced by Agkistrodon venom in mice     

K Sugimoto  N Sakurai  H Shirasawa  M Kaneko  Y Fujise  K Shibata  Y Komori  T Nikai  H Sugihara  Y Fukuda 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(2):255-262

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Isolation of Yersinia enterocolitica from some animals and meats.     

M Tsubokura  T Fukuda  K Otsuki  M Kubota  K Itagaki 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1975,37(2):213-215

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Age-related changes in the testes of horses     

Fukuda T  Kikuchi M  Kurotaki T  Oyamada T  Yoshikawa H  Yoshikawa T 《Equine veterinary journal》2001,33(1):20-25

Atrophy of seminiferous tubules and interstitial fibrosis are frequently observed in aged horses. Samples from 8 male Thoroughbreds, age 4-24 years, were subjected to histological, electron microscopical and immunohistochemical examination and statistical analysis. There were statistically significant increases in collagen fibres in the lamina propria of seminiferous tubules and testicular interstitium in 3 horses age 23 and 24 years compared with 5 horses age 4-20 years (P<0.001). Lamina propria surrounding atrophic tubules was thickened by an increase in collagen type IV and elastic fibres and by proliferation of bizarre myoid cells. Basal lamina was also thickened but had decreased reactivity for collagen type IV. Some myoid cells changed morphologically to a swollen and irregular shape and contained abundant cytoplasmic organelles. Laser scanning microscopy revealed that cytoplasmic actin filaments were decreased; the remaining filaments were positive for alpha-smooth muscle actin and vimentin, and matrix metalloproteinase-2 was secreted. These myoid cells transformed into myofibroblasts. The changes are interpreted as evidence of injured structure and function of the lamina propria and basal lamina and may explain the functional decline of the blood-testis barrier. Myoid cells may play an important role in the progression of testicular fibrosis.  相似文献   

Transcatheter closure of congenital ventricular septal defects in 3 dogs with a detachable coil   总被引：3，自引：0，他引：3  

Fujii Y  Fukuda T  Machida N  Yamane T  Wakao Y 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(6):911-914

The purpose of this study was to evaluate the feasibility, safety, and efficacy of transcatheter closure in dogs with a congenital perimembranous ventricular septal defect (VSD) by using a detachable coil. No dogs showed any symptoms, and results of chest X-rays and ECGs were normal. The diameters of VSD ranged from 2 to 4 mm on echocardiogram. The defect was 2-2.5 mm from the aortic valve. A detachable coil (size 5 mm with 5 loops) designed for patent ductus arteriosus was delivered via the transarterial route. The device was successfully employed in all dogs. A minimal residual shunt was observed in all dogs even though Qp/Qs decreased. Hemolysis and a rate-dependent right-bundle branch block were observed in 1 dog, but there was no clinical significance. No major complication was noted. Pathologic examination after 1 year revealed that the coils were covered with tissue without significant damage to the His-Purkinje conduction system. In conclusion, transcatheter closure of a small perimembranous VSD with a detachable coil can be achieved without major complications or significant pathologic damage at the lesion site.  相似文献